Rh2BG646100

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011712
Physical Location & Seq
Reverse (-)
86555403 .. 86557064
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG646100.1

Sequence Viewer

Length: 813 bp
ATGCAAAACGAAGATTACTCCGACCAGGGTTTGGTCTGTCCCAGCTTCAACAGCTACGCCTCGGCCACCACAGCCGACACCGCCGCTAAAGTTTGCCGGGAATTCAGCAGCCTTGGATTAGTGGATGAGAACAAAGAGTTCGAGTATGAACCACAACAACAACAAGAAGATGACTTCGAGTTCGTGTCGTTTCAGAAATCCGCAGACGAGGTTTTCTTCGGCGACAACACTCGGATCGGTCCGGTTTTCCCCGTCTTCAACCGCGACCTTATAAGTCAACGGGATCTAAACGACGACGTCGACGCCGTGCCGTCTTCGTCCTCAGCGTCATCAGACGTGGACGAGCTGGAGAACGTTCCGGCCGGTAGCTACTGCGTTTGGATGCCGAAAACGACGCCGGCCTCGTCTCCTTCCCATTGCAAGAAGAGCAAGTCGACCGGAACGTCGTCGTCTCGGCGGTGGAGGCTGAGGGACTTGCTGCGGCGGAGCAACAGCGAAGGCGGGAAGGACGCCTTCGTGCTGCTGACGCCTTCCCTGACATCATCGTCGAGCTCCAAGAAGCAGGTGGCTGCAGCTAACGATCATCATCATCATCCTAATGAGAAAATGGTGGTGACGAAGGGGAAGAAGAGCTTTAATGGGTCGAGGCCGAAAGGCCCGAACGCTGTGTCGATGGCGCACGAGGTGTTCTACGCGAAGAGTAAAGAGAAAGATGGGTATAATAAGAGACAGTCGTATCTCCCTTACAGGAAAGACCTGGTGGGGTTCTTTGCTAGTGTGAACGCCATGGGAAGGACTTTCCCTACTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

270

Amino Acids

29.98

Weight (kDa)

6.6

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 68 - 248 6.1e-23 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27880
fragaria_vesca FvH4_6g50800
malus_domestica MD09G1032200.v1.1
prunus_persica Prupe.3G285800_v2.0.a1
pyrus_communis pycom111g02550 pycom17g03100
rosa_chinensis RchiOBHm_Chr2g0172191
rosa_laevigata RLG00000022091 RLG00000035990
rosa_multiflora Rmu_ssc0000252.1_g000049
rosa_roxburghii Rroxscaffold_2G00121460
rosa_rugosa Rorug02G0556900
rosa_samantha Rh2AG632500 Rh2BG646100 Rh2CG612700 Rh2DG653500 Rh2DG660700 Rh5DG040300
rosa_wichuraiana Rw2G052360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AarI CACCTGC 1 cut(s) 553
AasI GACNNNNNNGTC 2 cut(s) 442, 544
AatII GACGTC 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 31
AccI GTMKAC 2 cut(s) 300, 434
AccII CGCG 2 cut(s) 264, 695
AciI CCGC 8 cut(s) 81, 84, 201, 262, 457, 481, 484, 501
AclI AACGTT 1 cut(s) 354
AclWI GGATC 2 cut(s) 242, 291
AcoI YGGCCR 2 cut(s) 63, 360
AcsI RAATTY 1 cut(s) 101
AcyI GRCGYC 5 cut(s) 297, 303, 395, 510, 527
AdeI CACNNNGTG 1 cut(s) 685
AfiI CCNNNNNNNGG 2 cut(s) 31, 792
AgsI TTSAA 2 cut(s) 49, 259
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 24, 756
AluBI AGCT 7 cut(s) 45, 54, 346, 369, 552, 575, 633
AluI AGCT 7 cut(s) 45, 54, 346, 369, 552, 575, 633
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 3 cut(s) 411, 456, 721
AlwI GGATC 2 cut(s) 242, 291
AoxI GGCC 5 cut(s) 63, 360, 399, 647, 655
ApeKI GCWGC 5 cut(s) 108, 478, 520, 569, 572
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 653, 685
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 2 cut(s) 239, 656
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 625
AvaII GGWCC 1 cut(s) 239
BaeI ACNNNNGTAYC 2 cut(s) 719, 752
BanII GRGCYC 1 cut(s) 554
BauI CACGAG 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 247, 306
Bbv12I GWGCWC 1 cut(s) 554
BbvCI CCTCAGC 2 cut(s) 322, 467
BbvI GCAGC 5 cut(s) 120, 465, 507, 556, 584
BccI CCATC 2 cut(s) 667, 707
BceAI ACGGC 2 cut(s) 290, 295
BciT130I CCWGG 2 cut(s) 26, 758
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 411, 456, 721
BfaI CTAG 1 cut(s) 774
BfmI CTRYAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 7 cut(s) 84, 109, 479, 482, 521, 570, 573
BlsI GCNGC 7 cut(s) 85, 110, 480, 483, 522, 571, 574
Bme1390I CCNGG 3 cut(s) 26, 98, 758
Bme18I GGWCC 1 cut(s) 239
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 239, 656
BmrFI CCNGG 3 cut(s) 26, 98, 758
BmsI GCATC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 247, 306
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 2 cut(s) 322, 467
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 5 cut(s) 297, 303, 395, 510, 527
BsaJI CCNNGG 4 cut(s) 25, 60, 112, 786
BsaWI WCCGGW 2 cut(s) 241, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 792
Bse118I RCCGGY 2 cut(s) 362, 397
Bse3DI GCAATG 1 cut(s) 415
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 2 cut(s) 26, 758
BseDI CCNNGG 4 cut(s) 25, 60, 112, 786
BseGI GGATG 3 cut(s) 130, 387, 592
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 31, 792
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 2 cut(s) 336, 458
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 5 cut(s) 120, 465, 507, 556, 584
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 2 cut(s) 264, 695
Bsh1285I CGRYCG 2 cut(s) 363, 438
BshFI GGCC 5 cut(s) 65, 362, 401, 649, 657
BsiEI CGRYCG 2 cut(s) 363, 438
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 6 cut(s) 97, 242, 359, 363, 398, 438
BslFI GGGAC 2 cut(s) 24, 485
BslI CCNNNNNNNGG 2 cut(s) 31, 792
BsmAI GTCTC 3 cut(s) 411, 456, 721
BsmBI CGTCTC 2 cut(s) 411, 456
BsmFI GGGAC 2 cut(s) 24, 485
BsnI GGCC 5 cut(s) 65, 362, 401, 649, 657
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 3 cut(s) 234, 283, 580
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 8 cut(s) 81, 84, 201, 262, 457, 481, 484, 501
BspANI GGCC 5 cut(s) 65, 362, 401, 649, 657
BspCNI CTCAG 2 cut(s) 335, 459
BspFNI CGCG 2 cut(s) 264, 695
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 2 cut(s) 242, 291
BspQI GCTCTTC 2 cut(s) 419, 623
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 2 cut(s) 362, 397
BssAI RCCGGY 2 cut(s) 362, 397
BssECI CCNNGG 4 cut(s) 25, 60, 112, 786
BssMI GATC 3 cut(s) 234, 283, 580
BssNI GRCGYC 5 cut(s) 297, 303, 395, 510, 527
BssSI CACGAG 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 112, 786
Bst2BI CACGAG 1 cut(s) 680
Bst2UI CCWGG 2 cut(s) 26, 758
Bst4CI ACNGT 1 cut(s) 732
Bst6I CTCTTC 3 cut(s) 419, 623, 692
BstACI GRCGYC 5 cut(s) 297, 303, 395, 510, 527
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 322, 467
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 3 cut(s) 130, 387, 592
BstFNI CGCG 2 cut(s) 264, 695
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 3 cut(s) 237, 286, 583
BstMAI GTCTC 3 cut(s) 411, 456, 721
BstMBI GATC 3 cut(s) 234, 283, 580
BstMCI CGRYCG 2 cut(s) 363, 438
BstMWI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 426, 463, 526
BstNI CCWGG 2 cut(s) 26, 758
BstSCI CCNGG 3 cut(s) 24, 96, 756
BstSFI CTRYAG 1 cut(s) 570
BstUI CGCG 2 cut(s) 264, 695
BstV1I GCAGC 5 cut(s) 120, 465, 507, 556, 584
BstV2I GAAGAC 2 cut(s) 247, 306
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 5 cut(s) 65, 362, 401, 649, 657
BtgI CCRYGG 1 cut(s) 786
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 3 cut(s) 130, 387, 592
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 2 cut(s) 362, 397
Cfr13I GGNCC 2 cut(s) 239, 656
CpoI CGGWCCG 1 cut(s) 239
CseI GACGC 5 cut(s) 311, 315, 403, 518, 535
CsiI ACCWGGT 1 cut(s) 756
CspI CGGWCCG 1 cut(s) 239
CviAII CATG 1 cut(s) 787
DdeI CTNAG 2 cut(s) 322, 467
DpnI GATC 3 cut(s) 236, 285, 582
DpnII GATC 3 cut(s) 234, 283, 580
DraIII CACNNNGTG 1 cut(s) 685
DrdI GACNNNNNNGTC 2 cut(s) 442, 544
DseDI GACNNNNNNGTC 2 cut(s) 442, 544
EaeI YGGCCR 2 cut(s) 63, 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 3 cut(s) 419, 623, 692
EarI CTCTTC 3 cut(s) 419, 623, 692
EciI GGCGGA 1 cut(s) 499
Ecl136II GAGCTC 1 cut(s) 552
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 112, 786
Eco24I GRGCYC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 360
Eco53kI GAGCTC 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 24, 756
EcoT14I CCWWGG 2 cut(s) 112, 786
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 2 cut(s) 112, 786
Esp3I CGTCTC 2 cut(s) 411, 456
FaeI CATG 1 cut(s) 790
FaiI YATR 4 cut(s) 147, 272, 720, 788
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 617, 649
FaqI GGGAC 2 cut(s) 24, 485
FatI CATG 1 cut(s) 786
FauI CCCGC 1 cut(s) 494
FblI GTMKAC 2 cut(s) 300, 434
Fnu4HI GCNGC 7 cut(s) 84, 109, 479, 482, 521, 570, 573
FokI GGATG 3 cut(s) 137, 394, 579
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 7 cut(s) 84, 109, 479, 482, 521, 570, 573
FspBI CTAG 1 cut(s) 774
GlaI GCGC 1 cut(s) 678
GluI GCNGC 7 cut(s) 84, 109, 479, 482, 521, 570, 573
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 5 cut(s) 65, 362, 401, 649, 657
HapII CCGG 6 cut(s) 97, 242, 359, 363, 398, 438
HgaI GACGC 5 cut(s) 311, 315, 403, 518, 535
HhaI GCGC 1 cut(s) 679
Hin1I GRCGYC 5 cut(s) 297, 303, 395, 510, 527
Hin1II CATG 1 cut(s) 790
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 3 cut(s) 278, 301, 435
HindII GTYRAC 3 cut(s) 278, 301, 435
HpaII CCGG 6 cut(s) 97, 242, 359, 363, 398, 438
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 5 cut(s) 278, 301, 340, 435, 781
Hpy188I TCNGA 4 cut(s) 22, 195, 234, 334
Hpy8I GTNNAC 5 cut(s) 278, 301, 340, 435, 781
Hpy99I CGWCG 8 cut(s) 296, 299, 302, 305, 397, 448, 451, 550
HpyAV CCTTC 7 cut(s) 420, 491, 499, 523, 540, 613, 786
HpyCH4III ACNGT 1 cut(s) 732
HpyCH4IV ACGT 4 cut(s) 297, 336, 354, 443
HpyCH4V TGCA 3 cut(s) 4, 420, 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 426, 463, 526
HpyF3I CTNAG 2 cut(s) 322, 467
HpySE526I ACGT 4 cut(s) 297, 336, 354, 443
Hsp92I GRCGYC 5 cut(s) 297, 303, 395, 510, 527
Hsp92II CATG 1 cut(s) 790
HspAI GCGC 1 cut(s) 677
KroI GCCGGC 1 cut(s) 397
KroNI GCCGGC 1 cut(s) 399
Kzo9I GATC 3 cut(s) 234, 283, 580
LguI GCTCTTC 2 cut(s) 419, 623
LmnI GCTCC 2 cut(s) 486, 557
Lsp1109I GCAGC 5 cut(s) 120, 465, 507, 556, 584
LweI GCATC 1 cut(s) 372
MabI ACCWGGT 1 cut(s) 756
MaeI CTAG 1 cut(s) 774
MaeII ACGT 4 cut(s) 297, 336, 354, 443
MaeIII GTNAC 1 cut(s) 613
MalI GATC 3 cut(s) 236, 285, 582
MboI GATC 3 cut(s) 234, 283, 580
MboII GAAGA 9 cut(s) 23, 179, 208, 247, 306, 436, 637, 640, 709
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 8 cut(s) 70, 202, 331, 412, 456, 462, 639, 676
MroNI GCCGGC 1 cut(s) 397
MseI TTAA 2 cut(s) 636, 811
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 6 cut(s) 97, 242, 359, 363, 398, 438
MspR9I CCNGG 3 cut(s) 26, 98, 758
MvaI CCWGG 2 cut(s) 26, 758
MvnI CGCG 2 cut(s) 264, 695
MwoI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 426, 463, 526
NaeI GCCGGC 1 cut(s) 399
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 786
NdeII GATC 3 cut(s) 234, 283, 580
NgoMIV GCCGGC 1 cut(s) 397
NlaIII CATG 1 cut(s) 790
NmeAIII GCCGAG 2 cut(s) 41, 433
NmuCI GTSAC 1 cut(s) 613
PaqCI CACCTGC 1 cut(s) 553
PciSI GCTCTTC 2 cut(s) 419, 623
PcsI WCGNNNNNNNCGW 5 cut(s) 297, 303, 323, 401, 440
PdiI GCCGGC 1 cut(s) 399
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 7 cut(s) 85, 110, 480, 483, 522, 571, 574
PsiI TTATAA 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 24, 756
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 24, 756
PspPI GGNCC 2 cut(s) 239, 656
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 597
Rsr2I CGGWCCG 1 cut(s) 239
RsrII CGGWCCG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 554
SalI GTCGAC 2 cut(s) 299, 433
SapI GCTCTTC 2 cut(s) 419, 623
SaqAI TTAA 2 cut(s) 636, 811
SatI GCNGC 7 cut(s) 84, 109, 479, 482, 521, 570, 573
Sau3AI GATC 3 cut(s) 234, 283, 580
Sau96I GGNCC 2 cut(s) 239, 656
ScrFI CCNGG 3 cut(s) 26, 98, 758
SduI GDGCHC 1 cut(s) 554
SexAI ACCWGGT 1 cut(s) 756
SfaNI GCATC 1 cut(s) 372
SfcI CTRYAG 1 cut(s) 570
SgrDI CGTCGACG 1 cut(s) 299
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 597
Sse9I AATT 1 cut(s) 101
SsiI CCGC 8 cut(s) 81, 84, 201, 262, 457, 481, 484, 501
SspMI CTAG 1 cut(s) 774
SstI GAGCTC 1 cut(s) 554
StyD4I CCNGG 3 cut(s) 24, 96, 756
StyI CCWWGG 2 cut(s) 112, 786
TaaI ACNGT 1 cut(s) 732
TaiI ACGT 4 cut(s) 300, 339, 357, 446
TaqI TCGA 7 cut(s) 141, 177, 300, 434, 548, 644, 671
TaqII GACCGA 1 cut(s) 227
TasI AATT 1 cut(s) 101
TauI GCSGC 2 cut(s) 86, 484
Tru1I TTAA 2 cut(s) 636, 811
Tru9I TTAA 2 cut(s) 636, 811
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 5 cut(s) 108, 478, 520, 569, 572
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 1 cut(s) 162
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 101
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 2 cut(s) 300, 434
XspI CTAG 1 cut(s) 774
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.