MD09G1032200.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011712
Physical Location & Seq
Forward (+)
1953682 .. 1954404
723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1032200.v1.1.491

Sequence Viewer

Length: 723 bp
ATGCAAAACGATGACGATTTCGAACAGGGATTCGTCTCTCCCAGCTTCAACAGCTACTCCACCGACAAACTAGCGGATGTCGCCGCCAAGGTCTGTCGGGAATTCGACCACCTCAATTTGCTCGAAGATTCGGAGTCCGATCAGTTGGGGGACCATAAAAACGACAATGAATCCGCCGCCGACGAGGAGGAGGAGGAGTTCGAATTCGTGTCGTTTCAGAAATCCGCCGACCAGGTTTTCTTCGACGAACACCAGATCGGCCCCGTTTTCCCCGTATTTAACCGCGACCTCCTGTCGGATAAAAGTCAACGCGATCTCCTCGCCGGCGGTCGGGATGGTAAAAAGGTGGAGGAGGACGATGATGGTCTGCCGTCGTCGTCGTCATCCGACGTGGACGAGCTGGACGAGGTCCCACCGGGGAATTACTGCGTGTGGATGTCGAAAGCCGCGTCGGGAGAAGCGCGTGGGAAGTGCAAGAAGAGCAAATCCACGGGAACTTCGTCGTCAAGGAGGTGGAGCCTCCGGGATTTGCTGCGGCGGAGCAACAGCGAAGGCGGCAAGGAGTTGGTTTTCTTGACGCCTTTGTCGTCCAGCTCCAAAAAAGTAGAAGACAACAAGGAACCCAAAAAGAGCTCCCGGTCGGCGTCTGGGTCTGATGTGCCCAGGAAAAAGCCCAGTAAAGGTCCAACGCGGTGTCGATGGCTCACGAGGCGTTCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

241

Amino Acids

26.78

Weight (kDa)

5.0

Isoelectric Point (pI)

62.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 81 - 223 4.6e-18 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27880
fragaria_vesca FvH4_6g50800
malus_domestica MD09G1032200.v1.1
prunus_persica Prupe.3G285800_v2.0.a1
pyrus_communis pycom111g02550 pycom17g03100
rosa_chinensis RchiOBHm_Chr2g0172191
rosa_laevigata RLG00000022091 RLG00000035990
rosa_multiflora Rmu_ssc0000252.1_g000049
rosa_roxburghii Rroxscaffold_2G00121460
rosa_rugosa Rorug02G0556900
rosa_samantha Rh2AG632500 Rh2BG646100 Rh2CG612700 Rh2DG653500 Rh2DG660700 Rh5DG040300
rosa_wichuraiana Rw2G052360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 583
AccII CGCG 5 cut(s) 285, 312, 449, 463, 691
AcsI RAATTY 2 cut(s) 101, 203
AcyI GRCGYC 2 cut(s) 578, 644
AfiI CCNNNNNNNGG 3 cut(s) 295, 330, 680
AgsI TTSAA 1 cut(s) 49
AhdI GACNNNNNGTC 1 cut(s) 292
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 231, 662
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 5 cut(s) 45, 54, 400, 594, 633
AluI AGCT 5 cut(s) 45, 54, 400, 594, 633
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 1 cut(s) 40
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 1 cut(s) 532
ApoI RAATTY 2 cut(s) 101, 203
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 4 cut(s) 151, 260, 409, 683
AsuC2I CCSGG 3 cut(s) 417, 524, 637
AsuII TTCGAA 2 cut(s) 21, 201
AvaII GGWCC 3 cut(s) 151, 409, 683
BaeGI GKGCMC 1 cut(s) 663
BanII GRGCYC 1 cut(s) 635
BauI CACGAG 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 1 cut(s) 519
BccI CCATC 3 cut(s) 329, 356, 693
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 2 cut(s) 233, 664
BcnI CCSGG 3 cut(s) 417, 524, 637
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 71
BisI GCNGC 6 cut(s) 84, 177, 447, 533, 536, 556
BlsI GCNGC 6 cut(s) 85, 178, 448, 534, 537, 557
Bme1390I CCNGG 5 cut(s) 233, 417, 524, 637, 664
Bme18I GGWCC 3 cut(s) 151, 409, 683
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 4 cut(s) 151, 260, 409, 683
BmiI GGNNCC 5 cut(s) 152, 262, 411, 518, 621
BmrFI CCNGG 5 cut(s) 233, 417, 524, 637, 664
BmrI ACTGGG 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 615
Bpu14I TTCGAA 2 cut(s) 21, 201
BpuMI CCSGG 3 cut(s) 417, 524, 637
BsaHI GRCGYC 2 cut(s) 578, 644
BsaJI CCNNGG 4 cut(s) 87, 416, 489, 662
BsaXI ACNNNNNCTCC 6 cut(s) 41, 71, 182, 212, 300, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 330, 680
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 675
BseBI CCWGG 2 cut(s) 233, 664
BseDI CCNNGG 4 cut(s) 87, 416, 489, 662
BseGI GGATG 4 cut(s) 82, 340, 383, 441
BseLI CCNNNNNNNGG 3 cut(s) 295, 330, 680
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 6 cut(s) 200, 203, 206, 209, 308, 365
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 1 cut(s) 519
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 5 cut(s) 285, 312, 449, 463, 691
Bsh1285I CGRYCG 2 cut(s) 331, 641
BshFI GGCC 1 cut(s) 261
BsiEI CGRYCG 2 cut(s) 331, 641
BsiHKAI GWGCWC 1 cut(s) 635
BsiSI CCGG 4 cut(s) 324, 416, 523, 637
BslFI GGGAC 2 cut(s) 164, 395
BslI CCNNNNNNNGG 3 cut(s) 295, 330, 680
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 2 cut(s) 164, 395
BsnI GGCC 1 cut(s) 261
Bsp119I TTCGAA 2 cut(s) 21, 201
Bsp1286I GDGCHC 2 cut(s) 635, 663
Bsp143I GATC 3 cut(s) 139, 255, 313
BspANI GGCC 1 cut(s) 261
BspFNI CGCG 5 cut(s) 285, 312, 449, 463, 691
BspLI GGNNCC 5 cut(s) 152, 262, 411, 518, 621
BspQI GCTCTTC 1 cut(s) 473
BspT104I TTCGAA 2 cut(s) 21, 201
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 1 cut(s) 675
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 87, 416, 489, 662
BssMI GATC 3 cut(s) 139, 255, 313
BssNI GRCGYC 2 cut(s) 578, 644
BssSI CACGAG 1 cut(s) 706
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 706
Bst2UI CCWGG 2 cut(s) 233, 664
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 2 cut(s) 578, 644
BstBI TTCGAA 2 cut(s) 21, 201
BstC8I GCNNGC 1 cut(s) 325
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 4 cut(s) 82, 340, 383, 441
BstFNI CGCG 5 cut(s) 285, 312, 449, 463, 691
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 3 cut(s) 142, 258, 316
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 139, 255, 313
BstMCI CGRYCG 2 cut(s) 331, 641
BstMWI GCNNNNNNNGC 5 cut(s) 51, 80, 480, 555, 709
BstNI CCWGG 2 cut(s) 233, 664
BstSCI CCNGG 5 cut(s) 231, 415, 522, 635, 662
BstSLI GKGCMC 1 cut(s) 663
BstUI CGCG 5 cut(s) 285, 312, 449, 463, 691
BstV1I GCAGC 1 cut(s) 519
BstV2I GAAGAC 1 cut(s) 615
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 489
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 4 cut(s) 82, 340, 383, 441
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 4 cut(s) 151, 260, 409, 683
CseI GACGC 3 cut(s) 438, 586, 633
CsiI ACCWGGT 1 cut(s) 231
DpnI GATC 3 cut(s) 141, 257, 315
DpnII GATC 3 cut(s) 139, 255, 313
DrdI GACNNNNNNGTC 1 cut(s) 583
DriI GACNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 473
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 473
EciI GGCGGA 3 cut(s) 163, 214, 553
Ecl136II GAGCTC 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 3 cut(s) 151, 409, 683
Eco53kI GAGCTC 1 cut(s) 633
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 409
EcoRI GAATTC 2 cut(s) 101, 203
EcoRII CCWGG 2 cut(s) 231, 662
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 635
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 40
FaiI YATR 2 cut(s) 156, 719
FaqI GGGAC 2 cut(s) 164, 395
Fnu4HI GCNGC 6 cut(s) 84, 177, 447, 533, 536, 556
FokI GGATG 4 cut(s) 89, 347, 370, 448
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 6 cut(s) 84, 177, 447, 533, 536, 556
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 462
GluI GCNGC 6 cut(s) 84, 177, 447, 533, 536, 556
GsaI CCCAGC 1 cut(s) 45
HaeIII GGCC 1 cut(s) 261
HapII CCGG 4 cut(s) 324, 416, 523, 637
HgaI GACGC 3 cut(s) 438, 586, 633
HhaI GCGC 1 cut(s) 463
Hin1I GRCGYC 2 cut(s) 578, 644
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 1 cut(s) 308
HindII GTYRAC 1 cut(s) 308
HinfI GANTC 4 cut(s) 30, 128, 134, 170
HpaII CCGG 4 cut(s) 324, 416, 523, 637
Hpy166II GTNNAC 2 cut(s) 308, 394
Hpy188I TCNGA 6 cut(s) 133, 139, 219, 298, 388, 655
Hpy188III TCNNGA 5 cut(s) 98, 332, 453, 574, 706
Hpy8I GTNNAC 2 cut(s) 308, 394
Hpy99I CGWCG 8 cut(s) 185, 248, 376, 379, 382, 392, 454, 505
HpyAV CCTTC 1 cut(s) 545
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 2 cut(s) 4, 474
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 80, 480, 555, 709
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 2 cut(s) 578, 644
HspAI GCGC 1 cut(s) 461
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 139, 255, 313
LguI GCTCTTC 1 cut(s) 473
LmnI GCTCC 4 cut(s) 516, 540, 599, 638
Lsp1109I GCAGC 1 cut(s) 519
MabI ACCWGGT 1 cut(s) 231
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 390
MalI GATC 3 cut(s) 141, 257, 315
MboI GATC 3 cut(s) 139, 255, 313
MboII GAAGA 4 cut(s) 137, 232, 490, 620
MhlI GDGCHC 2 cut(s) 635, 663
MluCI AATT 4 cut(s) 101, 115, 203, 421
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 3 cut(s) 276, 411, 710
MreI CGCCGGCG 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 323
MseI TTAA 1 cut(s) 279
MspI CCGG 4 cut(s) 324, 416, 523, 637
MspR9I CCNGG 5 cut(s) 233, 417, 524, 637, 664
MvaI CCWGG 2 cut(s) 233, 664
MvnI CGCG 5 cut(s) 285, 312, 449, 463, 691
MwoI GCNNNNNNNGC 5 cut(s) 51, 80, 480, 555, 709
NaeI GCCGGC 1 cut(s) 325
NciI CCSGG 3 cut(s) 417, 524, 637
NdeII GATC 3 cut(s) 139, 255, 313
NgoMIV GCCGGC 1 cut(s) 323
NlaIV GGNNCC 5 cut(s) 152, 262, 411, 518, 621
NspV TTCGAA 2 cut(s) 21, 201
PciSI GCTCTTC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 4 cut(s) 402, 446, 497, 584
PdiI GCCGGC 1 cut(s) 325
PfeI GAWTC 3 cut(s) 30, 128, 170
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 6 cut(s) 85, 178, 448, 534, 537, 557
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 231, 662
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 231, 662
PspN4I GGNNCC 5 cut(s) 152, 262, 411, 518, 621
PspPI GGNCC 4 cut(s) 151, 260, 409, 683
PspPPI RGGWCCY 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 407
SacI GAGCTC 1 cut(s) 635
SapI GCTCTTC 1 cut(s) 473
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 6 cut(s) 84, 177, 447, 533, 536, 556
Sau3AI GATC 3 cut(s) 139, 255, 313
Sau96I GGNCC 4 cut(s) 151, 260, 409, 683
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 5 cut(s) 233, 417, 524, 637, 664
SduI GDGCHC 2 cut(s) 635, 663
SexAI ACCWGGT 1 cut(s) 231
SfuI TTCGAA 2 cut(s) 21, 201
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 3 cut(s) 151, 409, 683
Sse9I AATT 4 cut(s) 101, 115, 203, 421
SspMI CTAG 1 cut(s) 71
SstI GAGCTC 1 cut(s) 635
StyD4I CCNGG 5 cut(s) 231, 415, 522, 635, 662
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 393
TaqI TCGA 7 cut(s) 21, 105, 123, 201, 243, 440, 697
TasI AATT 4 cut(s) 101, 115, 203, 421
TauI GCSGC 5 cut(s) 86, 179, 449, 538, 558
TfiI GAWTC 3 cut(s) 30, 128, 170
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TseI GCWGC 1 cut(s) 532
TspDTI ATGAA 1 cut(s) 183
Tth111I GACNNNGTC 1 cut(s) 407
VpaK11BI GGWCC 3 cut(s) 151, 409, 683
XapI RAATTY 2 cut(s) 101, 203
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.