FvH4_6g54310

Cofactor assembly of complex C subunit B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009124
Physical Location & Seq
Forward (+)
39688452 .. 39690128
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54310.t2

Sequence Viewer

Length: 813 bp
ATGGCAGCCGAAGTGTTATCATGGACTCCGATTGTTCCATCTCTCAGTCGTTCCAAAGCAGTTAAAGATATATCTCAGCAGCAGGCGCAACCATGGCTGAGTCGACGCTCAAGCGCCAAGAGGAGATGTGCAGTTCATGCATCCCTTGCCGAGTCTCTCATTGCTTCTTCTTCTTCTCCTCCTCCTCTTGTGCTTCTGGATCAATTCCATCAGCAGACACCATCGACCTCGCTGTTTCTGCTGGCTGCAGACACCGCCGGTTACTCCTTGGCCAGTTACTACACTTCTTTGGGTCTCTTCGTCATCTCTGTTCCAGGCCTTTGGTCCCTCATCAAACGTTCTGTTAAATCCAAGATTGTGCAGAAGACCTTTATCGGTGAAGAGAAGAAGGCACCGAATCAACTCGCGGGTGAAATTCTCTCCTTCTTCACCCGCAACAATTTTGTGGTCACTGATAGAGGAGAGACCATCACGTTTGAAGGTATGATGGTTCCAAGTCGAGGCCAAGCGGCATTGCTTACCTTTTGCACCTGCATCAGCCTCGCAAGTGTAGCCCTTGTTCTTACAATAACTGTTCCAGATTTCGGCAACAATTGGTTTTGGATCACCACTTTAAGTCCATTGGCGGGAGCATATTACTGGAAACGAGCCTCAAGAAAGGAGCAGATCAAGGTTAAAATGATAGTCATAGATGACGGTGCACAGTCAGAGGTTGTTGTTCAGGGGGATGACCAGCAAGTAGAGCAAATGAGGAAGGAGCTTAAACTGAGCGAGAAAGGGATGGTGTATGTCAAGGGTCTATTCGAGAGATAA

Protein Analysis

271

Amino Acids

29.86

Weight (kDa)

9.42

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB1 PF12046 92 - 209 3.7e-37 Cofactor assembly of complex C subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 539
Acc36I ACCTGC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 391
AccI GTMKAC 1 cut(s) 103
AccII CGCG 1 cut(s) 407
AciI CCGC 5 cut(s) 255, 407, 433, 509, 626
AclI AACGTT 1 cut(s) 337
AclWI GGATC 2 cut(s) 207, 611
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 414
AfiI CCNNNNNNNGG 3 cut(s) 500, 584, 626
AgsI TTSAA 1 cut(s) 479
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 1 cut(s) 760
AluI AGCT 1 cut(s) 760
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 3 cut(s) 159, 299, 458
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 2 cut(s) 207, 611
AoxI GGCC 3 cut(s) 270, 316, 502
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 3 cut(s) 5, 79, 245
ApoI RAATTY 1 cut(s) 414
AspLEI GCGC 2 cut(s) 88, 116
AspS9I GGNCC 1 cut(s) 324
AsuHPI GGTGA 4 cut(s) 389, 421, 422, 598
AvaII GGWCC 1 cut(s) 324
BaeGI GKGCMC 1 cut(s) 703
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 703
BbvI GCAGC 3 cut(s) 17, 91, 232
BccI CCATC 6 cut(s) 46, 216, 229, 476, 481, 775
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 3 cut(s) 159, 299, 458
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 539
BisI GCNGC 4 cut(s) 6, 80, 246, 510
BlsI GCNGC 4 cut(s) 7, 81, 247, 511
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 3 cut(s) 326, 393, 492
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 149, 543
BpiI GAAGAC 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 94, 637
BsaI GGTCTC 2 cut(s) 299, 458
BsaJI CCNNGG 2 cut(s) 92, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 500, 584, 626
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 273, 644
Bse3DI GCAATG 2 cut(s) 159, 512
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 92, 267
BseGI GGATG 3 cut(s) 140, 733, 786
BseLI CCNNNNNNNGG 3 cut(s) 500, 584, 626
BseMI GCAATG 2 cut(s) 159, 512
BseMII CTCAG 4 cut(s) 58, 89, 89, 758
BseNI ACTGG 2 cut(s) 273, 644
BseRI GAGGAG 5 cut(s) 136, 168, 171, 174, 474
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 3 cut(s) 17, 91, 232
BsgI GTGCAG 2 cut(s) 150, 380
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 3 cut(s) 272, 318, 504
BshNI GGYRCC 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 703
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 500, 584, 626
BsmAI GTCTC 3 cut(s) 159, 299, 458
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 3 cut(s) 272, 318, 504
Bso31I GGTCTC 2 cut(s) 299, 458
Bsp1286I GDGCHC 1 cut(s) 703
Bsp143I GATC 3 cut(s) 199, 603, 666
Bsp19I CCATGG 1 cut(s) 92
BspACI CCGC 5 cut(s) 255, 407, 433, 509, 626
BspANI GGCC 3 cut(s) 272, 318, 504
BspCNI CTCAG 4 cut(s) 57, 88, 90, 759
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 3 cut(s) 326, 393, 492
BspMAI CTGCAG 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 2 cut(s) 207, 611
BspT107I GGYRCC 1 cut(s) 391
BspTNI GGTCTC 2 cut(s) 299, 458
BsrDI GCAATG 2 cut(s) 159, 512
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 2 cut(s) 273, 644
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 92, 267
BssMI GATC 3 cut(s) 199, 603, 666
BssT1I CCWWGG 2 cut(s) 92, 267
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 574, 698, 705
Bst6I CTCTTC 2 cut(s) 302, 375
BstAPI GCANNNNNTGC 2 cut(s) 137, 146
BstC8I GCNNGC 2 cut(s) 84, 243
BstDEI CTNAG 4 cut(s) 44, 75, 98, 767
BstDSI CCRYGG 1 cut(s) 92
BstF5I GGATG 3 cut(s) 140, 733, 786
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 2 cut(s) 88, 116
BstKTI GATC 3 cut(s) 202, 606, 669
BstMAI GTCTC 3 cut(s) 159, 299, 458
BstMBI GATC 3 cut(s) 199, 603, 666
BstMWI GCNNNNNNNGC 8 cut(s) 85, 94, 137, 146, 238, 254, 551, 742
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 703
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 3 cut(s) 17, 91, 232
BstV2I GAAGAC 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 321
BsuRI GGCC 3 cut(s) 272, 318, 504
BtgI CCRYGG 1 cut(s) 92
BtsCI GGATG 3 cut(s) 140, 733, 786
BtsIMutI CAGTG 1 cut(s) 450
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 2 cut(s) 84, 243
CfoI GCGC 2 cut(s) 88, 116
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 324
CseI GACGC 1 cut(s) 114
CviAII CATG 3 cut(s) 21, 93, 137
DdeI CTNAG 4 cut(s) 44, 75, 98, 767
DpnI GATC 3 cut(s) 201, 605, 668
DpnII GATC 3 cut(s) 199, 603, 666
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 302, 375
EarI CTCTTC 2 cut(s) 302, 375
Eco130I CCWWGG 2 cut(s) 92, 267
Eco147I AGGCCT 1 cut(s) 318
Eco31I GGTCTC 2 cut(s) 299, 458
Eco47I GGWCC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 92, 267
EcoT22I ATGCAT 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 92, 267
FaeI CATG 3 cut(s) 24, 96, 140
FaiI YATR 8 cut(s) 22, 71, 94, 138, 485, 634, 689, 789
FaqI GGGAC 1 cut(s) 310
FatI CATG 3 cut(s) 20, 92, 136
FauI CCCGC 3 cut(s) 400, 440, 619
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 6, 80, 246, 510
FokI GGATG 3 cut(s) 127, 740, 793
Fsp4HI GCNGC 4 cut(s) 6, 80, 246, 510
GlaI GCGC 2 cut(s) 87, 115
GluI GCNGC 4 cut(s) 6, 80, 246, 510
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 3 cut(s) 272, 318, 504
HapII CCGG 1 cut(s) 258
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 88, 116
Hin1II CATG 3 cut(s) 24, 96, 140
Hin6I GCGC 2 cut(s) 86, 114
HinP1I GCGC 2 cut(s) 86, 114
HincII GTYRAC 1 cut(s) 104
HindII GTYRAC 1 cut(s) 104
HinfI GANTC 4 cut(s) 25, 100, 152, 397
HpaII CCGG 1 cut(s) 258
HphI GGTGA 4 cut(s) 389, 421, 422, 598
Hpy166II GTNNAC 2 cut(s) 104, 701
Hpy188I TCNGA 2 cut(s) 30, 709
Hpy188III TCNNGA 4 cut(s) 197, 578, 654, 805
Hpy8I GTNNAC 2 cut(s) 104, 701
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 4 cut(s) 382, 433, 473, 748
HpyCH4III ACNGT 3 cut(s) 574, 698, 705
HpyCH4IV ACGT 2 cut(s) 337, 473
HpyCH4V TGCA 7 cut(s) 131, 140, 248, 361, 528, 534, 701
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 94, 137, 146, 238, 254, 551, 742
HpyF3I CTNAG 4 cut(s) 44, 75, 98, 767
HpySE526I ACGT 2 cut(s) 337, 473
Hsp92II CATG 3 cut(s) 24, 96, 140
HspAI GCGC 2 cut(s) 86, 114
Kzo9I GATC 3 cut(s) 199, 603, 666
LmnI GCTCC 3 cut(s) 629, 661, 757
Lsp1109I GCAGC 3 cut(s) 17, 91, 232
LweI GCATC 2 cut(s) 149, 543
MaeII ACGT 2 cut(s) 337, 473
MaeIII GTNAC 3 cut(s) 260, 275, 448
MalI GATC 3 cut(s) 201, 605, 668
MboI GATC 3 cut(s) 199, 603, 666
MboII GAAGA 8 cut(s) 159, 162, 165, 289, 376, 392, 397, 418
MfeI CAATTG 1 cut(s) 592
MhlI GDGCHC 1 cut(s) 703
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 4 cut(s) 203, 414, 439, 592
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 3 cut(s) 19, 109, 161
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 142
MscI TGGCCA 1 cut(s) 272
MseI TTAA 5 cut(s) 63, 345, 614, 675, 762
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 592
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 8 cut(s) 85, 94, 137, 146, 238, 254, 551, 742
NcoI CCATGG 1 cut(s) 92
NdeII GATC 3 cut(s) 199, 603, 666
NlaIII CATG 3 cut(s) 24, 96, 140
NlaIV GGNNCC 3 cut(s) 326, 393, 492
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 142
PaqCI CACCTGC 1 cut(s) 539
PceI AGGCCT 1 cut(s) 318
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 4 cut(s) 7, 81, 247, 511
PleI GAGTC 3 cut(s) 19, 108, 160
PpsI GAGTC 3 cut(s) 19, 108, 160
Psp1406I AACGTT 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 326, 393, 492
PspPI GGNCC 1 cut(s) 324
PsrI GAACNNNNNNTAC 2 cut(s) 543, 575
PstI CTGCAG 1 cut(s) 250
SalI GTCGAC 1 cut(s) 102
SaqAI TTAA 5 cut(s) 63, 345, 614, 675, 762
SatI GCNGC 4 cut(s) 6, 80, 246, 510
Sau3AI GATC 3 cut(s) 199, 603, 666
Sau96I GGNCC 1 cut(s) 324
SchI GAGTC 3 cut(s) 19, 109, 161
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 703
SfaNI GCATC 2 cut(s) 149, 543
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 109, 652
SmoI CTYRAG 2 cut(s) 109, 652
Sse9I AATT 4 cut(s) 203, 414, 439, 592
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 5 cut(s) 255, 407, 433, 509, 626
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 92, 267
TaaI ACNGT 3 cut(s) 574, 698, 705
TaiI ACGT 2 cut(s) 340, 476
TaqI TCGA 4 cut(s) 103, 224, 499, 804
TasI AATT 4 cut(s) 203, 414, 439, 592
TauI GCSGC 1 cut(s) 512
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 5 cut(s) 63, 345, 614, 675, 762
Tru9I TTAA 5 cut(s) 63, 345, 614, 675, 762
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 3 cut(s) 5, 79, 245
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 1 cut(s) 125
TspRI CASTG 1 cut(s) 457
VneI GTGCAC 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 103
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.