MD17G1006700.v1.1

Cofactor assembly of complex C subunit B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009124
Physical Location & Seq
Reverse (-)
450047 .. 451754
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1006700.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCAGCGAAAGTGCTATCATCCCCTCCGCACCCGCATCCAAATCCTCTTAGTTCCCGTCCTCTGAAAATACTCCACGTAACCCACCACCAGCAGAGGCAGCAGCAGCCATGGCGGCTGAGCCAGCGTTCATCAGGAAGTTCAGGAACCAACAAGAAGAGAGAGGTGGTGATGAGCTCCCTCCACGAGTCTCTTGTTAGTACGTCGTCGTCTCTGGTACCTCTCTTACTGGAACCACCGCAGCAACTGAATGCATCCAGCAGCAGCATCAGCAGCTCCCTCATGTTTGTGGTTGCAGCTGCAGACGGCGCCGTAGGTTATTCGTTGGCCAGTTACTACACATCCCTGGGTCTCTTCGTCATCTCTGTTCCTGGCCTTTGGTCCCTCATCAAACGTTCTGTTAAATCCAAGGTCGTGCAGAAGACGTTTGTAGGTGAAGAGAAGAAGGCACCAAGCCAAGTCGCCGGTGAAATCCTCTCTTTTTTCACTCGCAACAATTTCGTGGTCACTGATCGAGGGGAGACCATTACGTTTGAAGGCATGATGGTTCCAAGTCGAGGCCAGGCGGCATTGCTCACTTTTTGCACCTGCATCAGCCTCGCAAGCGTAGCTCTTGTTCTTACCATAACTGTTCCCGATTTCGGCAATAACTGGTTTTGGCTCACCACCTTAAGTCCGTTGGCGGGCGCATATTACTGGAAGCGAGCGTCGCGAAAGGAGCAGATCAAGGTCAAAATGATGGTCATTGACGATGGTGAACAGTCAGAGATCATCGTTCAAGGGGATGACCAGCAAGTAGAGCAAATGAGAAAGGAGCTTCAACTGAGTGAGAAAGGAATGGTGTATGTTAAGGGCATATTCGAGAGATAA

Protein Analysis

290

Amino Acids

31.89

Weight (kDa)

9.49

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB1 PF12046 111 - 275 3.4e-57 Cofactor assembly of complex C subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 3 cut(s) 217, 308, 448
AccII CGCG 1 cut(s) 712
AciI CCGC 6 cut(s) 29, 35, 115, 239, 566, 683
AclI AACGTT 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 2 cut(s) 202, 219
AfiI CCNNNNNNNGG 3 cut(s) 557, 641, 683
AflII CTTAAG 1 cut(s) 670
AgsI TTSAA 3 cut(s) 536, 779, 821
AjnI CCWGG 3 cut(s) 345, 370, 561
AluBI AGCT 5 cut(s) 177, 276, 299, 611, 817
AluI AGCT 5 cut(s) 177, 276, 299, 611, 817
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 4 cut(s) 195, 216, 356, 515
AlwNI CAGNNNCTG 1 cut(s) 247
AoxI GGCC 3 cut(s) 327, 373, 559
ArsI GACNNNNNNTTYG 2 cut(s) 726, 758
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 2 cut(s) 311, 689
AspS9I GGNCC 1 cut(s) 381
AsuHPI GGTGA 5 cut(s) 181, 446, 479, 655, 767
AvaII GGWCC 1 cut(s) 381
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 3 cut(s) 217, 308, 448
BanII GRGCYC 1 cut(s) 179
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 179
BccI CCATC 3 cut(s) 538, 733, 746
BceAI ACGGC 2 cut(s) 296, 322
BcgI CGANNNNNNTGC 4 cut(s) 481, 515, 580, 614
BciT130I CCWGG 3 cut(s) 347, 372, 563
BcoDI GTCTC 4 cut(s) 195, 216, 356, 515
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 312
BfrI CTTAAG 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 596
BglI GCCNNNNNGGC 1 cut(s) 115
BlpI GCTNAGC 1 cut(s) 119
Bme1390I CCNGG 3 cut(s) 347, 372, 563
Bme18I GGWCC 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 7 cut(s) 148, 219, 234, 310, 383, 450, 549
BmrFI CCNGG 3 cut(s) 347, 372, 563
BmsI GCATC 4 cut(s) 46, 263, 276, 600
BpiI GAAGAC 1 cut(s) 428
Bpu1102I GCTNAGC 1 cut(s) 119
BsaAI YACGTR 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 2 cut(s) 356, 515
BsaJI CCNNGG 4 cut(s) 110, 345, 346, 408
Bsc4I CCNNNNNNNGG 3 cut(s) 557, 641, 683
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 4 cut(s) 234, 330, 656, 701
Bse3DI GCAATG 1 cut(s) 569
BseBI CCWGG 3 cut(s) 347, 372, 563
BseDI CCNNGG 4 cut(s) 110, 345, 346, 408
BseGI GGATG 5 cut(s) 20, 37, 254, 341, 790
BseLI CCNNNNNNNGG 3 cut(s) 557, 641, 683
BseMI GCAATG 1 cut(s) 569
BseMII CTCAG 2 cut(s) 110, 815
BseNI ACTGG 4 cut(s) 234, 330, 656, 701
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 1 cut(s) 712
BshFI GGCC 3 cut(s) 329, 375, 561
BshNI GGYRCC 3 cut(s) 217, 308, 448
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 557, 641, 683
BsmAI GTCTC 4 cut(s) 195, 216, 356, 515
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 3 cut(s) 329, 375, 561
Bso31I GGTCTC 2 cut(s) 356, 515
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 3 cut(s) 511, 723, 768
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 110
Bsp68I TCGCGA 1 cut(s) 712
BspACI CCGC 6 cut(s) 29, 35, 115, 239, 566, 683
BspANI GGCC 3 cut(s) 329, 375, 561
BspCNI CTCAG 2 cut(s) 111, 816
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 7 cut(s) 148, 219, 234, 310, 383, 450, 549
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 596
BspT107I GGYRCC 3 cut(s) 217, 308, 448
BspTI CTTAAG 1 cut(s) 670
BspTNI GGTCTC 2 cut(s) 356, 515
BsrDI GCAATG 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 4 cut(s) 234, 330, 656, 701
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 4 cut(s) 110, 345, 346, 408
BssMI GATC 3 cut(s) 511, 723, 768
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 110, 408
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 3 cut(s) 347, 372, 563
Bst4CI ACNGT 2 cut(s) 631, 762
Bst6I CTCTTC 3 cut(s) 152, 359, 432
BstACI GRCGYC 1 cut(s) 309
BstAFI CTTAAG 1 cut(s) 670
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 4 cut(s) 125, 604, 685, 705
BstDEI CTNAG 3 cut(s) 50, 119, 824
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 5 cut(s) 20, 37, 254, 341, 790
BstFNI CGCG 1 cut(s) 712
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 311, 689
BstKTI GATC 3 cut(s) 514, 726, 771
BstMAI GTCTC 4 cut(s) 195, 216, 356, 515
BstMBI GATC 3 cut(s) 511, 723, 768
BstNI CCWGG 3 cut(s) 347, 372, 563
BstSCI CCNGG 3 cut(s) 345, 370, 561
BstSFI CTRYAG 1 cut(s) 300
BstUI CGCG 1 cut(s) 712
BstV2I GAAGAC 1 cut(s) 428
BsuRI GGCC 3 cut(s) 329, 375, 561
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 5 cut(s) 20, 37, 254, 341, 790
BtsIMutI CAGTG 1 cut(s) 507
BtuMI TCGCGA 1 cut(s) 712
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 125, 604, 685, 705
CaiI CAGNNNCTG 1 cut(s) 247
CfoI GCGC 2 cut(s) 311, 689
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 381
CseI GACGC 1 cut(s) 696
Csp6I GTAC 2 cut(s) 201, 218
CviAII CATG 3 cut(s) 111, 283, 541
CviQI GTAC 2 cut(s) 201, 218
DdeI CTNAG 3 cut(s) 50, 119, 824
DinI GGCGCC 1 cut(s) 310
DpnI GATC 3 cut(s) 513, 725, 770
DpnII GATC 3 cut(s) 511, 723, 768
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 3 cut(s) 152, 359, 432
EarI CTCTTC 3 cut(s) 152, 359, 432
Ecl136II GAGCTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 110, 408
Eco24I GRGCYC 1 cut(s) 179
Eco31I GGTCTC 2 cut(s) 356, 515
Eco47I GGWCC 1 cut(s) 381
Eco53kI GAGCTC 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 177
EcoRII CCWGG 3 cut(s) 345, 370, 561
EcoT14I CCWWGG 2 cut(s) 110, 408
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 179
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
ErhI CCWWGG 2 cut(s) 110, 408
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 3 cut(s) 114, 286, 544
FaiI YATR 7 cut(s) 112, 284, 542, 626, 691, 846, 857
FaqI GGGAC 1 cut(s) 367
FatI CATG 3 cut(s) 110, 282, 540
FauI CCCGC 2 cut(s) 42, 676
FokI GGATG 5 cut(s) 7, 24, 241, 328, 797
FriOI GRGCYC 1 cut(s) 179
GlaI GCGC 2 cut(s) 310, 688
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 3 cut(s) 329, 375, 561
HapII CCGG 1 cut(s) 465
HgaI GACGC 1 cut(s) 696
HhaI GCGC 2 cut(s) 311, 689
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 3 cut(s) 114, 286, 544
Hin6I GCGC 2 cut(s) 309, 687
HinP1I GCGC 2 cut(s) 309, 687
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 465
HphI GGTGA 5 cut(s) 181, 446, 479, 655, 767
Hpy166II GTNNAC 1 cut(s) 758
Hpy188I TCNGA 2 cut(s) 66, 766
Hpy188III TCNNGA 5 cut(s) 135, 144, 635, 711, 862
Hpy8I GTNNAC 1 cut(s) 758
Hpy99I CGWCG 3 cut(s) 208, 211, 712
HpyAV CCTTC 2 cut(s) 439, 530
HpyCH4III ACNGT 2 cut(s) 631, 762
HpyCH4IV ACGT 5 cut(s) 78, 203, 394, 425, 530
HpyCH4V TGCA 6 cut(s) 254, 296, 302, 418, 585, 591
HpyF3I CTNAG 3 cut(s) 50, 119, 824
HpySE526I ACGT 5 cut(s) 78, 203, 394, 425, 530
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 3 cut(s) 114, 286, 544
HspAI GCGC 2 cut(s) 309, 687
KasI GGCGCC 1 cut(s) 308
KpnI GGTACC 1 cut(s) 221
Kzo9I GATC 3 cut(s) 511, 723, 768
LmnI GCTCC 4 cut(s) 182, 281, 718, 814
LweI GCATC 4 cut(s) 46, 263, 276, 600
MaeII ACGT 5 cut(s) 78, 203, 394, 425, 530
MaeIII GTNAC 3 cut(s) 79, 332, 505
MalI GATC 3 cut(s) 513, 725, 770
MboI GATC 3 cut(s) 511, 723, 768
MboII GAAGA 5 cut(s) 169, 346, 433, 449, 454
MhlI GDGCHC 1 cut(s) 179
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 496
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 1 cut(s) 197
Mox20I TGGCCA 1 cut(s) 329
Mph1103I ATGCAT 1 cut(s) 256
MscI TGGCCA 1 cut(s) 329
MseI TTAA 3 cut(s) 402, 671, 849
MslI CAYNNNNRTG 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 299
MspCI CTTAAG 1 cut(s) 670
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 3 cut(s) 347, 372, 563
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 347, 372, 563
MvnI CGCG 1 cut(s) 712
NarI GGCGCC 1 cut(s) 309
NcoI CCATGG 1 cut(s) 110
NdeII GATC 3 cut(s) 511, 723, 768
NlaIII CATG 3 cut(s) 114, 286, 544
NlaIV GGNNCC 7 cut(s) 148, 219, 234, 310, 383, 450, 549
NmuCI GTSAC 1 cut(s) 505
NruI TCGCGA 1 cut(s) 712
NsiI ATGCAT 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 596
PasI CCCWGGG 1 cut(s) 346
PctI GAATGC 1 cut(s) 256
PleI GAGTC 1 cut(s) 196
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 79
Psp124BI GAGCTC 1 cut(s) 179
Psp1406I AACGTT 1 cut(s) 394
Psp6I CCWGG 3 cut(s) 345, 370, 561
PspGI CCWGG 3 cut(s) 345, 370, 561
PspN4I GGNNCC 7 cut(s) 148, 219, 234, 310, 383, 450, 549
PspPI GGNCC 1 cut(s) 381
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 299
RruI TCGCGA 1 cut(s) 712
RsaI GTAC 2 cut(s) 202, 219
RsaNI GTAC 2 cut(s) 201, 218
RseI CAYNNNNRTG 1 cut(s) 287
SacI GAGCTC 1 cut(s) 179
SaqAI TTAA 3 cut(s) 402, 671, 849
Sau3AI GATC 3 cut(s) 511, 723, 768
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 3 cut(s) 347, 372, 563
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 4 cut(s) 46, 263, 276, 600
SfcI CTRYAG 1 cut(s) 300
SfoI GGCGCC 1 cut(s) 310
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
Sse9I AATT 1 cut(s) 496
SsiI CCGC 6 cut(s) 29, 35, 115, 239, 566, 683
SspDI GGCGCC 1 cut(s) 308
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 3 cut(s) 345, 370, 561
StyI CCWWGG 2 cut(s) 110, 408
TaaI ACNGT 2 cut(s) 631, 762
TaiI ACGT 5 cut(s) 81, 206, 397, 428, 533
TaqI TCGA 3 cut(s) 514, 556, 861
TasI AATT 1 cut(s) 496
TauI GCSGC 2 cut(s) 118, 569
Tru1I TTAA 3 cut(s) 402, 671, 849
Tru9I TTAA 3 cut(s) 402, 671, 849
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 514
Vha464I CTTAAG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 381
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.