Rh2DG698800

Cofactor assembly of complex C subunit B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009124
Physical Location & Seq
Forward (+)
91414647 .. 91418249
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG698800.1

Sequence Viewer

Length: 621 bp
ATGGCAGCTAAAGTGTTTTCATGGACTCCGAGTATTCCATCTCTCAGTCCTTCCAAATTACGCCAAGCTCAGCCGCAGCAGCCATGGCTGAGTCGACGCTCAAGCGCCAAGAAGAGATCTGCACTGGTCGTTCGTGCCTCCCTTCCCGAGTCTCTCATGGCTTCTTCTCCTCCTCCTGTTCTGCTACTGGAACAACTCCATCAGCAGACACCATCAACCTCGCTGTTTCTGCTGGCTGCAGACACCGCCGGTTACTCCTTGGCCAGTTACTACACTTCTTTGGGTCTCTTCGTCATCTCTGTTCCAGGCCTTTGGTCCCTCATCAAACGTTCTGTTAAATCCAAGATTGTGCAGAAGACATTTATTGGGGAAGAGAAGAAGGCACCAAATCAAGTCGCAGGTGAAATTCTCTCCTTCTTCACTCGCAACAATTTTGTGGTCACTGATAGAGGAGAGACCATCACGTTTGAAGGCATGATGGTTCCAAGTCGAGGCCAAGCAGCACTGCTGACCTTTTGCACCTGCATCAGCCTCGCAAGTGTAGCCCTTGTTCTTACAATAACTGTTCCAGATTTCGGCAACAATTGGTTTTGGATCACCACCTTAAGTCCATTGGCGTAA

Protein Analysis

206

Amino Acids

22.49

Weight (kDa)

9.95

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB1 PF12046 89 - 206 3.9e-37 Cofactor assembly of complex C subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 389, 530
Acc36I ACCTGC 2 cut(s) 389, 530
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 94
AciI CCGC 2 cut(s) 74, 246
AclI AACGTT 1 cut(s) 328
AclWI GGATC 1 cut(s) 602
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 405
AfiI CCNNNNNNNGG 2 cut(s) 491, 575
AflII CTTAAG 1 cut(s) 604
AgsI TTSAA 1 cut(s) 470
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 2 cut(s) 8, 68
AluI AGCT 2 cut(s) 8, 68
Alw26I GTCTC 3 cut(s) 156, 290, 449
AlwI GGATC 1 cut(s) 602
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 3 cut(s) 261, 307, 493
ApeKI GCWGC 5 cut(s) 5, 76, 79, 236, 500
ApoI RAATTY 1 cut(s) 405
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 413, 589
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 1 cut(s) 315
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 362
BbvI GCAGC 5 cut(s) 17, 88, 91, 223, 512
BccI CCATC 5 cut(s) 46, 207, 220, 467, 472
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 156, 290, 449
BfmI CTRYAG 1 cut(s) 237
BfoI RGCGCY 1 cut(s) 108
BfrI CTTAAG 1 cut(s) 604
BfuAI ACCTGC 2 cut(s) 389, 530
BglII AGATCT 1 cut(s) 116
BisI GCNGC 6 cut(s) 6, 74, 77, 80, 237, 501
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 6 cut(s) 7, 75, 78, 81, 238, 502
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 3 cut(s) 317, 384, 483
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 534
BpiI GAAGAC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 85
BsaI GGTCTC 2 cut(s) 290, 449
BsaJI CCNNGG 2 cut(s) 83, 258
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 575
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 3 cut(s) 129, 192, 264
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 83, 258
BseLI CCNNNNNNNGG 2 cut(s) 491, 575
BseMII CTCAG 3 cut(s) 58, 80, 83
BseNI ACTGG 3 cut(s) 129, 192, 264
BseRI GAGGAG 3 cut(s) 159, 162, 465
BseXI GCAGC 5 cut(s) 17, 88, 91, 223, 512
BsgI GTGCAG 2 cut(s) 105, 371
BshFI GGCC 3 cut(s) 263, 309, 495
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 491, 575
BsmAI GTCTC 3 cut(s) 156, 290, 449
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 3 cut(s) 263, 309, 495
Bso31I GGTCTC 2 cut(s) 290, 449
BsoBI CYCGRG 1 cut(s) 146
Bsp143I GATC 2 cut(s) 116, 594
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 1 cut(s) 83
BspACI CCGC 2 cut(s) 74, 246
BspANI GGCC 3 cut(s) 263, 309, 495
BspCNI CTCAG 3 cut(s) 57, 81, 82
BspLI GGNNCC 3 cut(s) 317, 384, 483
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 2 cut(s) 389, 530
BspPI GGATC 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 604
BspTNI GGTCTC 2 cut(s) 290, 449
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 3 cut(s) 129, 192, 264
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 83, 258
BssMI GATC 2 cut(s) 116, 594
BssT1I CCWWGG 2 cut(s) 83, 258
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 565
Bst6I CTCTTC 3 cut(s) 107, 293, 366
BstAFI CTTAAG 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 3 cut(s) 44, 69, 89
BstDSI CCRYGG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 119, 597
BstMAI GTCTC 3 cut(s) 156, 290, 449
BstMBI GATC 2 cut(s) 116, 594
BstMWI GCNNNNNNNGC 5 cut(s) 79, 85, 229, 245, 542
BstNI CCWGG 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 5 cut(s) 17, 88, 91, 223, 512
BstV2I GAAGAC 1 cut(s) 362
BstX2I RGATCY 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 312
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 3 cut(s) 263, 309, 495
BtgI CCRYGG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 3 cut(s) 122, 441, 503
BveI ACCTGC 2 cut(s) 389, 530
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 1 cut(s) 105
CviAII CATG 4 cut(s) 21, 84, 157, 475
DdeI CTNAG 3 cut(s) 44, 69, 89
DpnI GATC 2 cut(s) 118, 596
DpnII GATC 2 cut(s) 116, 594
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 3 cut(s) 107, 293, 366
EarI CTCTTC 3 cut(s) 107, 293, 366
Eco130I CCWWGG 2 cut(s) 83, 258
Eco147I AGGCCT 1 cut(s) 309
Eco31I GGTCTC 2 cut(s) 290, 449
Eco47I GGWCC 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 2 cut(s) 83, 258
ErhI CCWWGG 2 cut(s) 83, 258
FaeI CATG 4 cut(s) 24, 87, 160, 478
FaiI YATR 4 cut(s) 22, 85, 158, 476
FaqI GGGAC 1 cut(s) 301
FatI CATG 4 cut(s) 20, 83, 156, 474
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 6 cut(s) 6, 74, 77, 80, 237, 501
Fsp4HI GCNGC 6 cut(s) 6, 74, 77, 80, 237, 501
GlaI GCGC 1 cut(s) 106
GluI GCNGC 6 cut(s) 6, 74, 77, 80, 237, 501
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 3 cut(s) 263, 309, 495
HapII CCGG 1 cut(s) 249
HgaI GACGC 1 cut(s) 105
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 24, 87, 160, 478
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 95
HindII GTYRAC 1 cut(s) 95
HinfI GANTC 3 cut(s) 25, 91, 149
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 413, 589
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 2 cut(s) 146, 569
Hpy8I GTNNAC 1 cut(s) 95
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 5 cut(s) 60, 152, 373, 424, 464
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 2 cut(s) 328, 464
HpyCH4V TGCA 5 cut(s) 122, 239, 352, 519, 525
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 85, 229, 245, 542
HpyF3I CTNAG 3 cut(s) 44, 69, 89
HpySE526I ACGT 2 cut(s) 328, 464
Hsp92II CATG 4 cut(s) 24, 87, 160, 478
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 116, 594
Lsp1109I GCAGC 5 cut(s) 17, 88, 91, 223, 512
LweI GCATC 1 cut(s) 534
MaeII ACGT 2 cut(s) 328, 464
MaeIII GTNAC 3 cut(s) 251, 266, 439
MalI GATC 2 cut(s) 118, 596
MboI GATC 2 cut(s) 116, 594
MboII GAAGA 7 cut(s) 124, 156, 280, 367, 383, 388, 409
MfeI CAATTG 1 cut(s) 583
MflI RGATCY 1 cut(s) 116
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 4 cut(s) 56, 405, 430, 583
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 3 cut(s) 19, 100, 158
MnlI CCTC 8 cut(s) 148, 180, 183, 229, 329, 443, 485, 542
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
MseI TTAA 2 cut(s) 336, 605
Msp20I TGGCCA 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 306
MunI CAATTG 1 cut(s) 583
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 5 cut(s) 79, 85, 229, 245, 542
NcoI CCATGG 1 cut(s) 83
NdeII GATC 2 cut(s) 116, 594
NlaIII CATG 4 cut(s) 24, 87, 160, 478
NlaIV GGNNCC 3 cut(s) 317, 384, 483
NmuCI GTSAC 1 cut(s) 439
PaqCI CACCTGC 2 cut(s) 389, 530
PceI AGGCCT 1 cut(s) 309
PkrI GCNGC 6 cut(s) 7, 75, 78, 81, 238, 502
PleI GAGTC 3 cut(s) 19, 99, 157
PpsI GAGTC 3 cut(s) 19, 99, 157
Psp1406I AACGTT 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 317, 384, 483
PspPI GGNCC 1 cut(s) 315
PsrI GAACNNNNNNTAC 2 cut(s) 534, 566
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 116
SalI GTCGAC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 336, 605
SatI GCNGC 6 cut(s) 6, 74, 77, 80, 237, 501
Sau3AI GATC 2 cut(s) 116, 594
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 3 cut(s) 19, 100, 158
ScrFI CCNGG 1 cut(s) 306
SetI ASST 9 cut(s) 10, 70, 221, 331, 403, 467, 515, 524, 605
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 100, 604
SmoI CTYRAG 2 cut(s) 100, 604
Sse9I AATT 4 cut(s) 56, 405, 430, 583
SseBI AGGCCT 1 cut(s) 309
SsiI CCGC 2 cut(s) 74, 246
StuI AGGCCT 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 2 cut(s) 83, 258
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 2 cut(s) 331, 467
TaqI TCGA 2 cut(s) 94, 490
TasI AATT 4 cut(s) 56, 405, 430, 583
TauI GCSGC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 336, 605
Tru9I TTAA 2 cut(s) 336, 605
TscAI CASTG 3 cut(s) 129, 448, 510
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 5 cut(s) 5, 76, 79, 236, 500
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 1 cut(s) 9
TspRI CASTG 3 cut(s) 129, 448, 510
Vha464I CTTAAG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.