FvH4_7g13130

Protein-ribulosamine 3-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005372
Physical Location & Seq
Reverse (-)
11837236 .. 11841880
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13130.t2

Sequence Viewer

Length: 996 bp
ATGACAATGGCGGGTCACGTGGCACTCATATCGTTCTCTTCTTCCTTACCTTCTCTGCCTGGTCTTCCTCGTCTGTCCTCCACAAAGCACCGACCTTTAATCATGGCGGCAATGAGTGATGATCCAGTTCGTAAATGGATTCTCACGGACGGAAATGCAACAGAGATCATAAGAATTAGTCCTATAGGCGGAGGTTGCATCAATCGTGCTAGTCGTTATGACACTGATGCTGGTTCTTTCTTTGTTAAAACAAACAGGAGCATTGGACCATCTATGTTTGAGGGAGAGGCTCTTGGCTTAGGTGCCATGTTTAATACCGGATCAATTCGTGTCCCTAAGCCATTCAAGGTTGGCCCTCTACCTACCGGTGGTTCTTACATCATTATGGAGTTTATTGACTTTGGTTTATCCAGAGGTAATCAGTCTGATCTAGGCAGGAAGCTTGCTGAGATGCATAAGACTGGAAAATCTGAAAAAGGTTTTGGTTTTGAAGTTGACAATACCATAGGCAGCACTCCGCAGATAAACACTTGGTCATCAGATTGGATTCAGTTTTATGGGGAGCATAGACTGGGTTATCAGTTGCAGCTGGCTAGGGACCGGTATGGTGACAGTACCATATATGAAAAAGGACAAAGATTAGTGAAGAGTATGGGACCTCTCTTTGATAATGTAGTGATTGAGCCATGCCTGTTACATGGGGACTTATGGAGTGGAAATATCAGCTCTGACAAGAAAGGCGAGCCAGTTATACTTGACCCGGCATGTTACTATGGACATAATGAGGCGGAATTTGGAATGTCATGGTGTGCTGGCTTTGGCGGATCTTTCTACAGTTCCTATTTTGAGGTGATGCCCAAGCAACCAGGGTTTGAGCAAAGGAAAGATCTATACATGTTGTATCATTACTTGAATCATTATAACCTCTTTGGTTCTGGTTACCGTTCCTCTGCCATGTCCATAATAGACGACTATCTTCGGATGTTAAAAGCTTAG

Protein Analysis

332

Amino Acids

36.74

Weight (kDa)

7.08

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fructosamin_kin PF03881 1 - 236 6.5e-88 Fructosamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
AccB1I GGYRCC 1 cut(s) 302
AciI CCGC 6 cut(s) 11, 107, 189, 518, 788, 822
AclWI GGATC 3 cut(s) 116, 328, 832
AcsI RAATTY 1 cut(s) 791
AcvI CACGTG 1 cut(s) 19
AfaI GTAC 1 cut(s) 616
AfiI CCNNNNNNNGG 2 cut(s) 188, 368
AflIII ACRYGT 1 cut(s) 894
AgeI ACCGGT 2 cut(s) 365, 600
AgsI TTSAA 3 cut(s) 346, 491, 913
AjnI CCWGG 2 cut(s) 58, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 4 cut(s) 442, 589, 726, 992
AluI AGCT 4 cut(s) 442, 589, 726, 992
AlwI GGATC 3 cut(s) 116, 328, 832
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 2 cut(s) 510, 586
ApoI RAATTY 1 cut(s) 791
AsiGI ACCGGT 2 cut(s) 365, 600
AspS9I GGNCC 4 cut(s) 266, 353, 598, 656
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 2 cut(s) 620, 862
AvaII GGWCC 3 cut(s) 266, 598, 656
BanI GGYRCC 1 cut(s) 302
BbrPI CACGTG 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 522, 598
BccI CCATC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 2 cut(s) 60, 867
BcnI CCSGG 1 cut(s) 761
BfaI CTAG 3 cut(s) 210, 431, 594
BfmI CTRYAG 2 cut(s) 183, 832
BglII AGATCT 1 cut(s) 886
BisI GCNGC 3 cut(s) 108, 511, 587
BlsI GCNGC 3 cut(s) 109, 512, 588
Bme1390I CCNGG 3 cut(s) 60, 761, 867
Bme18I GGWCC 3 cut(s) 266, 598, 656
BmgT120I GGNCC 4 cut(s) 266, 353, 598, 656
BmiI GGNNCC 3 cut(s) 304, 599, 657
BmrFI CCNGG 3 cut(s) 60, 761, 867
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 4 cut(s) 207, 217, 441, 843
BmuI ACTGGG 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 56
Bpu10I CCTNAGC 2 cut(s) 298, 336
BpuMI CCSGG 1 cut(s) 761
BsaAI YACGTR 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 866
BsaWI WCCGGW 3 cut(s) 317, 365, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 368
Bse118I RCCGGY 2 cut(s) 365, 600
Bse1I ACTGG 4 cut(s) 125, 466, 576, 746
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 2 cut(s) 60, 867
BseDI CCNNGG 1 cut(s) 866
BseGI GGATG 1 cut(s) 987
BseLI CCNNNNNNNGG 2 cut(s) 188, 368
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 4 cut(s) 125, 466, 576, 746
BseXI GCAGC 2 cut(s) 522, 598
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 1 cut(s) 302
BshTI ACCGGT 2 cut(s) 365, 600
BsiSI CCGG 4 cut(s) 318, 366, 601, 761
BslFI GGGAC 4 cut(s) 317, 611, 669, 716
BslI CCNNNNNNNGG 2 cut(s) 188, 368
BsmFI GGGAC 4 cut(s) 317, 611, 669, 716
BsnI GGCC 1 cut(s) 354
Bsp143I GATC 6 cut(s) 121, 165, 320, 427, 824, 886
BspACI CCGC 6 cut(s) 11, 107, 189, 518, 788, 822
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 304, 599, 657
BspPI GGATC 3 cut(s) 116, 328, 832
BspT107I GGYRCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 117
BsrFI RCCGGY 2 cut(s) 365, 600
BsrI ACTGG 4 cut(s) 125, 466, 576, 746
BssAI RCCGGY 2 cut(s) 365, 600
BssECI CCNNGG 1 cut(s) 866
BssMI GATC 6 cut(s) 121, 165, 320, 427, 824, 886
Bst2UI CCWGG 2 cut(s) 60, 867
Bst4CI ACNGT 3 cut(s) 614, 836, 944
Bst6I CTCTTC 2 cut(s) 43, 641
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 4 cut(s) 444, 591, 743, 814
BstDEI CTNAG 4 cut(s) 298, 336, 447, 993
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 1 cut(s) 987
BstKTI GATC 6 cut(s) 124, 168, 323, 430, 827, 889
BstMBI GATC 6 cut(s) 121, 165, 320, 427, 824, 886
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 2 cut(s) 60, 867
BstNSI RCATGY 2 cut(s) 768, 898
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 3 cut(s) 58, 759, 865
BstSFI CTRYAG 2 cut(s) 183, 832
BstV1I GCAGC 2 cut(s) 522, 598
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 824, 886
BstYI RGATCY 2 cut(s) 824, 886
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 1 cut(s) 987
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 4 cut(s) 444, 591, 743, 814
Cfr10I RCCGGY 2 cut(s) 365, 600
Cfr13I GGNCC 4 cut(s) 266, 353, 598, 656
Csp6I GTAC 1 cut(s) 615
CspAI ACCGGT 2 cut(s) 365, 600
CviAII CATG 8 cut(s) 103, 307, 687, 698, 765, 804, 895, 955
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 4 cut(s) 298, 336, 447, 993
DpnI GATC 6 cut(s) 123, 167, 322, 429, 826, 888
DpnII GATC 6 cut(s) 121, 165, 320, 427, 824, 886
Eam1104I CTCTTC 2 cut(s) 43, 641
EarI CTCTTC 2 cut(s) 43, 641
EciI GGCGGA 3 cut(s) 204, 803, 837
Eco47I GGWCC 3 cut(s) 266, 598, 656
Eco72I CACGTG 1 cut(s) 19
Eco91I GGTNACC 1 cut(s) 938
EcoO109I RGGNCCY 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 938
EcoRII CCWGG 2 cut(s) 58, 865
EcoT22I ATGCAT 1 cut(s) 456
FaeI CATG 8 cut(s) 106, 310, 690, 701, 768, 807, 898, 958
FaqI GGGAC 4 cut(s) 317, 611, 669, 716
FatI CATG 8 cut(s) 102, 306, 686, 697, 764, 803, 894, 954
FauI CCCGC 1 cut(s) 4
Fnu4HI GCNGC 3 cut(s) 108, 511, 587
Fsp4HI GCNGC 3 cut(s) 108, 511, 587
FspBI CTAG 3 cut(s) 210, 431, 594
GluI GCNGC 3 cut(s) 108, 511, 587
HaeIII GGCC 1 cut(s) 354
HapII CCGG 4 cut(s) 318, 366, 601, 761
Hin1II CATG 8 cut(s) 106, 310, 690, 701, 768, 807, 898, 958
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 2 cut(s) 440, 990
HinfI GANTC 3 cut(s) 139, 547, 913
HpaII CCGG 4 cut(s) 318, 366, 601, 761
HphI GGTGA 2 cut(s) 620, 862
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 5 cut(s) 427, 472, 541, 730, 981
Hpy188III TCNNGA 1 cut(s) 411
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 3 cut(s) 614, 836, 944
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 158, 198, 454, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 4 cut(s) 298, 336, 447, 993
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 8 cut(s) 106, 310, 690, 701, 768, 807, 898, 958
Kzo9I GATC 6 cut(s) 121, 165, 320, 427, 824, 886
LmnI GCTCC 2 cut(s) 258, 562
Lsp1109I GCAGC 2 cut(s) 522, 598
LweI GCATC 4 cut(s) 207, 217, 441, 843
MaeI CTAG 3 cut(s) 210, 431, 594
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 5 cut(s) 14, 608, 693, 767, 938
MalI GATC 6 cut(s) 123, 167, 322, 429, 826, 888
MboI GATC 6 cut(s) 121, 165, 320, 427, 824, 886
MboII GAAGA 5 cut(s) 30, 33, 56, 658, 968
MflI RGATCY 2 cut(s) 824, 886
MluCI AATT 3 cut(s) 174, 324, 791
Mph1103I ATGCAT 1 cut(s) 456
MseI TTAA 4 cut(s) 98, 246, 312, 986
MslI CAYNNNNRTG 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 4 cut(s) 318, 366, 601, 761
MspR9I CCNGG 3 cut(s) 60, 761, 867
MvaI CCWGG 2 cut(s) 60, 867
MwoI GCNNNNNNNGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 761
NdeII GATC 6 cut(s) 121, 165, 320, 427, 824, 886
NlaIII CATG 8 cut(s) 106, 310, 690, 701, 768, 807, 898, 958
NlaIV GGNNCC 3 cut(s) 304, 599, 657
NmuCI GTSAC 2 cut(s) 14, 608
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 2 cut(s) 768, 898
PciI ACATGT 1 cut(s) 894
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PfeI GAWTC 3 cut(s) 139, 547, 913
PinAI ACCGGT 2 cut(s) 365, 600
PkrI GCNGC 3 cut(s) 109, 512, 588
PmaCI CACGTG 1 cut(s) 19
PmlI CACGTG 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 656
PscI ACATGT 1 cut(s) 894
PsiI TTATAA 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 58, 865
PspCI CACGTG 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 938
PspGI CCWGG 2 cut(s) 58, 865
PspN4I GGNNCC 3 cut(s) 304, 599, 657
PspPI GGNCC 4 cut(s) 266, 353, 598, 656
PspPPI RGGWCCY 1 cut(s) 656
PsuI RGATCY 2 cut(s) 824, 886
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 4 cut(s) 98, 246, 312, 986
SatI GCNGC 3 cut(s) 108, 511, 587
Sau3AI GATC 6 cut(s) 121, 165, 320, 427, 824, 886
Sau96I GGNCC 4 cut(s) 266, 353, 598, 656
ScrFI CCNGG 3 cut(s) 60, 761, 867
SfaNI GCATC 4 cut(s) 207, 217, 441, 843
SfcI CTRYAG 2 cut(s) 183, 832
SinI GGWCC 3 cut(s) 266, 598, 656
SmiMI CAYNNNNRTG 1 cut(s) 383
Sse9I AATT 3 cut(s) 174, 324, 791
SsiI CCGC 6 cut(s) 11, 107, 189, 518, 788, 822
SspMI CTAG 3 cut(s) 210, 431, 594
StyD4I CCNGG 3 cut(s) 58, 759, 865
TaaI ACNGT 3 cut(s) 614, 836, 944
TaiI ACGT 1 cut(s) 21
TasI AATT 3 cut(s) 174, 324, 791
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 3 cut(s) 139, 547, 913
Tru1I TTAA 4 cut(s) 98, 246, 312, 986
Tru9I TTAA 4 cut(s) 98, 246, 312, 986
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 2 cut(s) 14, 608
TseI GCWGC 2 cut(s) 510, 586
Tsp45I GTSAC 2 cut(s) 14, 608
TspDTI ATGAA 1 cut(s) 639
TspGWI ACGGA 2 cut(s) 161, 165
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 3 cut(s) 266, 598, 656
XapI RAATTY 1 cut(s) 791
XceI RCATGY 2 cut(s) 768, 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XspI CTAG 3 cut(s) 210, 431, 594
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.