Rorug01G0226200

Protein-ribulosamine 3-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005372
Physical Location & Seq
Reverse (-)
32553771 .. 32557147
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0226200.1

Sequence Viewer

Length: 2976 bp
ATGGGGTTATCCCTTTTCTTATTGGTATCCATGTGTATGCTTTCAAGTGTTCTGTTATTTCATGTTGTGGTTGCCAACAATTTCTCTTCTTTGCTGCAATCTTGCCATGAAGAGGAGAGGTTTGCCTTGCTACAGTTCAAGGAAAGCTTTATCATCAATTCATCTGTTCCGATCATTGGTGCTAATCCAAAGATTTTATCATGGAAACTGGGTGGCAAAAATAACAACTGCTGTTCATGGGATGGTGTCGAATGTGATGAGAAGACAGGTCATGTGATTGCTCTTGATCTTAGTAGCAGTTGTCTCTATGGCTCTATCAACTCCACTAGTAGCCTCTTCCGTCTTGTTCATCTTCAGAGGCTGAACCTTGCAAACAATTACTTCAATTACTCTCAAATTCCTTCTGCCATTAGGAATTTTCCAAGGCTCAGGTATCTTAACCTCTCTAACTCTATCTTTTCTGGTGAAGTCCCATCTGAAGTTTCACTGTTATCAAGGGTTGGCTCTGTCTTTCCTGGTGAAACATTGTTGAAACTGAATGCATCCACTATGAGAAGACTAGTTCAAAACTTAACCAGTCTTCAAAAGCTTCATCTCAGTTTTATTAACATATCCTCGCCAGTACCCGATTCCATTGCAAATTTATCGTTTTTGACATCTCTCCTCCTTGAAGATTGTGCGCTCTTTGGTGAATTTCCCCTAAGAATTTTTAAATTGCAAAGCTTAAAAACTCTTAGTGTGAGGTTCAACCAAGATCTCGTTGGTCATGTGCCTGAATTTAATAGAAGTAGTCCTCTCGTGTCATTGGGACTTGCCAGCACTAGCTTTTCCGGAAGCCTACCTTCTTCGATTGAAAAGCTTGATTCATTGAATTTCTTGGATATCAGTGGATGCAGTTTCTCAGGGGTGATTCCATTTTCAGTTGGTAATCTTAAGCAGCTCAATTATTTGCGCCTTTCAGAAAATAGATTTACTGGTCCAGTCCCTGCGTCCTTAGCAAACCTCACCCAACTTACATATCTGTCACTTTCTTATAATAATCTTAGTGCTGGTCCCTTGTCTTGGGTTGGTAAGCAAACCAAACTTGCTAGTCTAGGACTTCGAGGCATCAATTTAAGTGGTTACATCCCGTCTTCTCTTAGCAACCTCACAGAGCTCAATTATCTTAACCTTGGTGTTAATGAGCTAACCGGCCCAATCCCATCTTCGCTAGGTAACCTTAGCAGACTAGTTGGCATAAAACTTTTTCAAAATAAATTTTATGGACCAATTCCCGAGTTATTCTACAATTTCATAAACCTCCAAACCCTTGAGCTAGATCGCAATAGTCTGAGCGGTGTTGTGGAGTTTGACGGGTTTCTCAAGTTACAAAATGTCACCGAAATCATGTTATCTGATAACAAATTGGAGTTGCTCACTGAGTCCAGATCTCCAAATGCAAGTGTTCCACAACTCAGTAGTCTAGGGTTGAGATCATGCAGCATAAGAGAGTTTCCAGATTTCCTAAGATTCCAACAAAAATTGCAGTGGCTGGACCTTGCTGCAAACAATTTGCAGGGGCCAGTACCGAAATGGATGTGGAACACAAGCACCGAAACTTTGCAGATGCTGCTCATTCATGACAACTTCCTTTCAGGCTTTGGCCAACCTCAAGCTGACCAACATCTAGTTGTTCTTCCCTGGCTTAACTTACGTCTGTTGAGTTTTGTGAACAACTCGCTACAGGGACCCTTACCAATACCTCCACCATGCATCGAATATTACTGCGCTAGGGATAACAAACTATCTGGAGAAATTTCACCATTGATCTGTCATCTGACTTCTCTTCAGTTGCTTGACTTGTCAAATAACAAGTTGAGTGGCATGCTTCCACAGTGTCTTGGATACTTTAGTGACTATCTGCAGGTCTTAAGACTTGGAAACAACTCTTTTCAAGGCATTCTTCCCCAAGTGTACAGCCAGAGAAGCATACTCAAGATGATTGATGTTAGTCACAATCAATTACAGGGGAAATTACCAAGGTCATTGGCAAACTGTGTAATGCTCGAGTTTCTTGTTCTCTCAAACAATGATTTCAGTGATGTATTCCCTTTTTGGTTGGGGACTCTTCCGGAGTTAAAAGTTTTGGCATTGCGCAATAATGGCTTCCATGGTGTGTTAGGAAAACCTGAATACAGTCGTGGTTTCTCCCAGTTGCGCATTTTTGATCTGTCTTACAATAATTTCAAAGGTGAGTTTCTGATTGACTACATCTTTTCTGAGAAGGCTATGACGAGAGTGGACATCAGTGTAAACCAGTCGACATATATGAAGGCAGACATTAATTTGGTGGTTTGTGGCTTTTCAAGCATTGCCTGGTTTCCCATCTCAATGGACATTGCAGTTAAAGGTTTGGAGTTGTACTATCCAAAGATTCAAGAAGCCTTTGCAGTCATTGATATCTCAAGCAATAGATTTGAAGGGAGGATTCATGAATTCATTGGGAATCTTAAGGGGCTTCGCTCTCTCAACGTCTCAAATAACATTTTCACTGGTTCCATTCCATCGTTCTTGGGGAACTTAACACTGCTTGAATCATTGGATGTTTCCCAAAACAAGCTCTCAGGGGAGATTCCTCAGCAACTGGTGCAGCTCACTTTCCTTGCCAAGTTCAATGTTTCCCACAACAAGCTCACCGGTCCTATACCACATGGAACCCAGCTCACTTCTTTCGATATCACTTCCTACGAGTGCAACTCAGGATTGTGTGGAGATCCATTGCCGGAGAAATGTGGAAATCCTTCACTTCCACCTTCAAGTGTAGAAGAAAATGATCCAATTGAATTTGATTGGAAGTTTGTTGTTGCGGGGTTTGGAAGTGGGTTGGTTGTAGGAGTAGTTCTTGCAGACCTTTTCATCACAAGGTTTCGTGAATGGTTTATCGAGATTATTGGAATGATCACACCAATGAAAAGGAGAAGACAGAAGAGAAACTAA

Protein Analysis

991

Amino Acids

110.31

Weight (kDa)

7.17

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 86 1.5e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 268 - 360 4.9e-10 Leucine-rich repeat region
LRR_8 PF13855 307 - 348 3.8e-06 Leucine rich repeat
LRR_8 PF13855 634 - 692 1.1e-08 Leucine rich repeat
LRR_14 PF23598 811 - 891 7.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
Acc16I TGCGCA 2 cut(s) 2135, 2198
Acc36I ACCTGC 1 cut(s) 1894
AccBSI CCGCTC 1 cut(s) 1335
AccI GTMKAC 1 cut(s) 2300
AccIII TCCGGA 2 cut(s) 830, 2110
AciI CCGC 2 cut(s) 1335, 2846
AclWI GGATC 2 cut(s) 2747, 2807
AcoI YGGCCR 1 cut(s) 1642
AcuI CTGAAG 3 cut(s) 338, 498, 1811
AfaI GTAC 4 cut(s) 624, 1566, 1955, 2402
AfiI CCNNNNNNNGG 3 cut(s) 112, 176, 1062
AflII CTTAAG 3 cut(s) 932, 1909, 2489
AgeI ACCGGT 1 cut(s) 2675
AhlI ACTAGT 3 cut(s) 326, 559, 1228
AjnI CCWGG 3 cut(s) 514, 1679, 2354
AjuI GAANNNNNNNTTGG 2 cut(s) 1189, 1221
Alw21I GWGCWC 1 cut(s) 1158
Alw26I GTCTC 2 cut(s) 308, 2518
AlwI GGATC 2 cut(s) 2747, 2807
AlwNI CAGNNNCTG 5 cut(s) 361, 986, 1531, 1609, 2623
Ama87I CYCGRG 2 cut(s) 1274, 2045
Aor13HI TCCGGA 2 cut(s) 830, 2110
AoxI GGCC 3 cut(s) 1192, 1559, 1642
ApeKI GCWGC 6 cut(s) 94, 937, 1479, 1541, 1609, 2629
AseI ATTAAT 1 cut(s) 2322
AsiGI ACCGGT 1 cut(s) 2675
Asp700I GAANNNNTTC 1 cut(s) 2779
AspLEI GCGC 5 cut(s) 682, 954, 1769, 2136, 2199
AspS9I GGNCC 8 cut(s) 977, 1052, 1193, 1265, 1534, 1559, 1727, 2678
AsuHPI GGTGA 9 cut(s) 476, 530, 701, 919, 997, 1369, 1791, 2243, 2665
AvaI CYCGRG 2 cut(s) 1274, 2045
AvaII GGWCC 6 cut(s) 977, 1052, 1265, 1534, 1727, 2678
BaeI ACNNNNGTAYC 2 cut(s) 1876, 1909
BalI TGGCCA 1 cut(s) 1644
BanII GRGCYC 1 cut(s) 1158
BauI CACGAG 1 cut(s) 797
BbsI GAAGAC 5 cut(s) 269, 562, 572, 1125, 2965
Bbv12I GWGCWC 1 cut(s) 1158
BbvCI CCTCAGC 1 cut(s) 2616
BbvI GCAGC 6 cut(s) 81, 949, 1491, 1528, 1596, 2641
BccI CCATC 5 cut(s) 236, 481, 1210, 2372, 2551
BcgI CGANNNNNNTGC 4 cut(s) 617, 627, 651, 661
BciT130I CCWGG 3 cut(s) 516, 1681, 2356
BciVI GTATCC 2 cut(s) 37, 1877
BclI TGATCA 1 cut(s) 2937
BcoDI GTCTC 2 cut(s) 308, 2518
BcuI ACTAGT 3 cut(s) 326, 559, 1228
BfmI CTRYAG 3 cut(s) 131, 1721, 1901
BfrI CTTAAG 3 cut(s) 932, 1909, 2489
BfuAI ACCTGC 1 cut(s) 1894
BfuI GTATCC 2 cut(s) 37, 1877
BglII AGATCT 2 cut(s) 754, 1427
BisI GCNGC 6 cut(s) 95, 938, 1480, 1542, 1610, 2630
BlsI GCNGC 6 cut(s) 96, 939, 1481, 1543, 1611, 2631
Bme1390I CCNGG 3 cut(s) 516, 1681, 2356
Bme18I GGWCC 6 cut(s) 977, 1052, 1265, 1534, 1727, 2678
BmeT110I CYCGRG 2 cut(s) 1274, 2045
BmgT120I GGNCC 8 cut(s) 977, 1052, 1193, 1265, 1534, 1559, 1727, 2678
BmiI GGNNCC 6 cut(s) 1054, 1560, 1728, 1729, 2536, 2695
BmrFI CCNGG 3 cut(s) 516, 1681, 2356
BmrI ACTGGG 2 cut(s) 218, 2185
BmsI GCATC 5 cut(s) 551, 881, 1116, 1596, 1761
BmuI ACTGGG 2 cut(s) 218, 2185
BpiI GAAGAC 5 cut(s) 269, 562, 572, 1125, 2965
BplI GAGNNNNNCTC 2 cut(s) 2720, 2752
BpmI CTGGAG 1 cut(s) 1809
Bpu10I CCTNAGC 4 cut(s) 428, 994, 1220, 2616
BpuEI CTTGAG 5 cut(s) 1331, 1346, 1635, 1958, 2428
BsaJI CCNNGG 5 cut(s) 422, 1171, 1679, 2018, 2149
BsaWI WCCGGW 3 cut(s) 830, 2110, 2675
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 176, 1062
Bse118I RCCGGY 2 cut(s) 1190, 2675
Bse3DI GCAATG 5 cut(s) 633, 2129, 2349, 2376, 2756
BseAI TCCGGA 2 cut(s) 830, 2110
BseBI CCWGG 3 cut(s) 516, 1681, 2356
BseDI CCNNGG 5 cut(s) 422, 1171, 1679, 2018, 2149
BseGI GGATG 6 cut(s) 247, 542, 896, 1125, 1581, 2587
BseLI CCNNNNNNNGG 3 cut(s) 112, 176, 1062
BseMI GCAATG 5 cut(s) 633, 2129, 2349, 2376, 2756
BseRI GAGGAG 2 cut(s) 128, 653
BseXI GCAGC 6 cut(s) 81, 949, 1491, 1528, 1596, 2641
BseYI CCCAGC 1 cut(s) 2697
BsgI GTGCAG 1 cut(s) 2648
BshFI GGCC 3 cut(s) 1194, 1561, 1644
BshTI ACCGGT 1 cut(s) 2675
BsiHKAI GWGCWC 1 cut(s) 1158
BsiHKCI CYCGRG 2 cut(s) 1274, 2045
BsiSI CCGG 5 cut(s) 831, 1191, 2111, 2676, 2762
BslFI GGGAC 6 cut(s) 455, 822, 968, 1038, 1740, 2116
BslI CCNNNNNNNGG 3 cut(s) 112, 176, 1062
BsmAI GTCTC 2 cut(s) 308, 2518
BsmBI CGTCTC 1 cut(s) 2518
BsmFI GGGAC 6 cut(s) 455, 822, 968, 1038, 1740, 2116
BsmI GAATGC 2 cut(s) 544, 1938
BsnI GGCC 3 cut(s) 1194, 1561, 1644
BsoBI CYCGRG 2 cut(s) 1274, 2045
Bsp1286I GDGCHC 1 cut(s) 1158
Bsp13I TCCGGA 2 cut(s) 830, 2110
Bsp1407I TGTACA 1 cut(s) 1953
Bsp19I CCATGG 1 cut(s) 2149
BspACI CCGC 2 cut(s) 1335, 2846
BspANI GGCC 3 cut(s) 1194, 1561, 1644
BspEI TCCGGA 2 cut(s) 830, 2110
BspHI TCATGA 2 cut(s) 1618, 2470
BspLI GGNNCC 6 cut(s) 1054, 1560, 1728, 1729, 2536, 2695
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1894
BspPI GGATC 2 cut(s) 2747, 2807
BspTI CTTAAG 3 cut(s) 932, 1909, 2489
BsrBI CCGCTC 1 cut(s) 1335
BsrDI GCAATG 5 cut(s) 633, 2129, 2349, 2376, 2756
BsrFI RCCGGY 2 cut(s) 1190, 2675
BsrGI TGTACA 1 cut(s) 1953
BssAI RCCGGY 2 cut(s) 1190, 2675
BssECI CCNNGG 5 cut(s) 422, 1171, 1679, 2018, 2149
BssSI CACGAG 1 cut(s) 797
BssT1I CCWWGG 4 cut(s) 422, 1171, 2018, 2149
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 3 cut(s) 516, 1681, 2356
Bst4CI ACNGT 5 cut(s) 135, 489, 1875, 2036, 2177
Bst6I CTCTTC 6 cut(s) 91, 105, 341, 1830, 2112, 2960
BstAFI CTTAAG 3 cut(s) 932, 1909, 2489
BstAPI GCANNNNNTGC 2 cut(s) 1609, 2626
BstAUI TGTACA 1 cut(s) 1953
BstC8I GCNNGC 2 cut(s) 817, 1865
BstDSI CCRYGG 1 cut(s) 2149
BstEII GGTNACC 1 cut(s) 1214
BstF5I GGATG 6 cut(s) 247, 542, 896, 1125, 1581, 2587
BstHHI GCGC 5 cut(s) 682, 954, 1769, 2136, 2199
BstMAI GTCTC 2 cut(s) 308, 2518
BstMWI GCNNNNNNNGC 6 cut(s) 995, 1609, 1965, 2142, 2346, 2626
BstNI CCWGG 3 cut(s) 516, 1681, 2356
BstNSI RCATGY 1 cut(s) 1867
BstPI GGTNACC 1 cut(s) 1214
BstSCI CCNGG 3 cut(s) 514, 1679, 2354
BstSFI CTRYAG 3 cut(s) 131, 1721, 1901
BstV1I GCAGC 6 cut(s) 81, 949, 1491, 1528, 1596, 2641
BstV2I GAAGAC 5 cut(s) 269, 562, 572, 1125, 2965
BstX2I RGATCY 3 cut(s) 754, 1427, 2752
BstXI CCANNNNNNTGG 1 cut(s) 2371
BstYI RGATCY 3 cut(s) 754, 1427, 2752
BsuI GTATCC 2 cut(s) 37, 1877
BsuRI GGCC 3 cut(s) 1194, 1561, 1644
BtgI CCRYGG 1 cut(s) 2149
BtsCI GGATG 6 cut(s) 247, 542, 896, 1125, 1581, 2587
BtsI GCAGTG 2 cut(s) 1532, 2564
BtsIMutI CAGTG 9 cut(s) 485, 892, 1416, 1532, 1880, 2083, 2293, 2529, 2564
BveI ACCTGC 1 cut(s) 1894
Cac8I GCNNGC 2 cut(s) 817, 1865
CaiI CAGNNNCTG 5 cut(s) 361, 986, 1531, 1609, 2623
CciI TCATGA 2 cut(s) 1618, 2470
CfoI GCGC 5 cut(s) 682, 954, 1769, 2136, 2199
Cfr10I RCCGGY 2 cut(s) 1190, 2675
Cfr13I GGNCC 8 cut(s) 977, 1052, 1193, 1265, 1534, 1559, 1727, 2678
CseI GACGC 1 cut(s) 978
Csp6I GTAC 4 cut(s) 623, 1565, 1954, 2401
CspAI ACCGGT 1 cut(s) 2675
CspCI CAANNNNNGTGG 4 cut(s) 1099, 1134, 1840, 1875
CviQI GTAC 4 cut(s) 623, 1565, 1954, 2401
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 1642
Eam1104I CTCTTC 6 cut(s) 91, 105, 341, 1830, 2112, 2960
EarI CTCTTC 6 cut(s) 91, 105, 341, 1830, 2112, 2960
Ecl136II GAGCTC 1 cut(s) 1156
Eco130I CCWWGG 4 cut(s) 422, 1171, 2018, 2149
Eco24I GRGCYC 1 cut(s) 1158
Eco32I GATATC 3 cut(s) 883, 2440, 2716
Eco47I GGWCC 6 cut(s) 977, 1052, 1265, 1534, 1727, 2678
Eco53kI GAGCTC 1 cut(s) 1156
Eco57I CTGAAG 3 cut(s) 338, 498, 1811
Eco88I CYCGRG 2 cut(s) 1274, 2045
Eco91I GGTNACC 1 cut(s) 1214
EcoICRI GAGCTC 1 cut(s) 1156
EcoO109I RGGNCCY 1 cut(s) 1727
EcoO65I GGTNACC 1 cut(s) 1214
EcoRI GAATTC 1 cut(s) 2474
EcoRII CCWGG 3 cut(s) 514, 1679, 2354
EcoRV GATATC 3 cut(s) 883, 2440, 2716
EcoT14I CCWWGG 4 cut(s) 422, 1171, 2018, 2149
EcoT22I ATGCAT 2 cut(s) 544, 1754
EcoT38I GRGCYC 1 cut(s) 1158
ErhI CCWWGG 4 cut(s) 422, 1171, 2018, 2149
Esp3I CGTCTC 1 cut(s) 2518
FalI AAGNNNNNCTT 6 cut(s) 131, 163, 826, 858, 1926, 1958
FaqI GGGAC 6 cut(s) 455, 822, 968, 1038, 1740, 2116
FauI CCCGC 1 cut(s) 2839
FbaI TGATCA 1 cut(s) 2937
FblI GTMKAC 1 cut(s) 2300
Fnu4HI GCNGC 6 cut(s) 95, 938, 1480, 1542, 1610, 2630
FokI GGATG 6 cut(s) 254, 529, 903, 1112, 1588, 2594
FriOI GRGCYC 1 cut(s) 1158
Fsp4HI GCNGC 6 cut(s) 95, 938, 1480, 1542, 1610, 2630
FspI TGCGCA 2 cut(s) 2135, 2198
GlaI GCGC 5 cut(s) 681, 953, 1768, 2135, 2198
GluI GCNGC 6 cut(s) 95, 938, 1480, 1542, 1610, 2630
GsaI CCCAGC 1 cut(s) 2701
GsuI CTGGAG 1 cut(s) 1809
HaeIII GGCC 3 cut(s) 1194, 1561, 1644
HapII CCGG 5 cut(s) 831, 1191, 2111, 2676, 2762
HgaI GACGC 1 cut(s) 978
HhaI GCGC 5 cut(s) 682, 954, 1769, 2136, 2199
Hin6I GCGC 5 cut(s) 680, 952, 1767, 2134, 2197
HinP1I GCGC 5 cut(s) 680, 952, 1767, 2134, 2197
HincII GTYRAC 1 cut(s) 2301
HindII GTYRAC 1 cut(s) 2301
HindIII AAGCTT 4 cut(s) 145, 587, 721, 857
HpaII CCGG 5 cut(s) 831, 1191, 2111, 2676, 2762
HphI GGTGA 9 cut(s) 476, 530, 701, 919, 997, 1369, 1791, 2243, 2665
Hpy166II GTNNAC 5 cut(s) 1711, 1954, 2281, 2293, 2301
Hpy188I TCNGA 9 cut(s) 171, 357, 478, 961, 1332, 1396, 1818, 2241, 2260
Hpy8I GTNNAC 5 cut(s) 1711, 1954, 2281, 2293, 2301
HpyAV CCTTC 7 cut(s) 411, 852, 2257, 2305, 2453, 2790, 2802
HpyCH4III ACNGT 5 cut(s) 135, 489, 1875, 2036, 2177
HpyCH4IV ACGT 2 cut(s) 1693, 2511
HpyF10VI GCNNNNNNNGC 6 cut(s) 995, 1609, 1965, 2142, 2346, 2626
HpySE526I ACGT 2 cut(s) 1693, 2511
HspAI GCGC 5 cut(s) 680, 952, 1767, 2134, 2197
KflI GGGWCCC 1 cut(s) 1727
Kpn2I TCCGGA 2 cut(s) 830, 2110
Ksp22I TGATCA 1 cut(s) 2937
Lsp1109I GCAGC 6 cut(s) 81, 949, 1491, 1528, 1596, 2641
LweI GCATC 5 cut(s) 551, 881, 1116, 1596, 1761
MaeII ACGT 2 cut(s) 1693, 2511
MaeIII GTNAC 7 cut(s) 1023, 1121, 1214, 1365, 1375, 1892, 1991
MbiI CCGCTC 1 cut(s) 1335
MfeI CAATTG 1 cut(s) 2817
MflI RGATCY 3 cut(s) 754, 1427, 2752
MhlI GDGCHC 1 cut(s) 1158
MlsI TGGCCA 1 cut(s) 1644
MluNI TGGCCA 1 cut(s) 1644
MlyI GAGTC 2 cut(s) 1430, 2098
MmeI TCCRAC 1 cut(s) 1537
Mox20I TGGCCA 1 cut(s) 1644
Mph1103I ATGCAT 2 cut(s) 544, 1754
MroI TCCGGA 2 cut(s) 830, 2110
MroXI GAANNNNTTC 1 cut(s) 2779
MscI TGGCCA 1 cut(s) 1644
MslI CAYNNNNRTG 3 cut(s) 35, 2369, 2945
Msp20I TGGCCA 1 cut(s) 1644
MspCI CTTAAG 3 cut(s) 932, 1909, 2489
MspI CCGG 5 cut(s) 831, 1191, 2111, 2676, 2762
MspR9I CCNGG 3 cut(s) 516, 1681, 2356
MunI CAATTG 1 cut(s) 2817
Mva1269I GAATGC 2 cut(s) 544, 1938
MvaI CCWGG 3 cut(s) 516, 1681, 2356
MwoI GCNNNNNNNGC 6 cut(s) 995, 1609, 1965, 2142, 2346, 2626
NcoI CCATGG 1 cut(s) 2149
NlaIV GGNNCC 6 cut(s) 1054, 1560, 1728, 1729, 2536, 2695
NmuCI GTSAC 4 cut(s) 1023, 1375, 1892, 1991
NsbI TGCGCA 2 cut(s) 2135, 2198
NsiI ATGCAT 2 cut(s) 544, 1754
NspI RCATGY 1 cut(s) 1867
PaeI GCATGC 1 cut(s) 1867
PaeR7I CTCGAG 1 cut(s) 2045
PagI TCATGA 2 cut(s) 1618, 2470
PctI GAATGC 2 cut(s) 544, 1938
PdmI GAANNNNTTC 1 cut(s) 2779
PfeI GAWTC 9 cut(s) 629, 863, 910, 1509, 2413, 2467, 2485, 2573, 2611
PinAI ACCGGT 1 cut(s) 2675
PkrI GCNGC 6 cut(s) 96, 939, 1481, 1543, 1611, 2631
PleI GAGTC 2 cut(s) 1429, 2098
PpsI GAGTC 2 cut(s) 1429, 2098
PpuMI RGGWCCY 1 cut(s) 1727
PshBI ATTAAT 1 cut(s) 2322
PsiI TTATAA 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 1727
Psp6I CCWGG 3 cut(s) 514, 1679, 2354
PspEI GGTNACC 1 cut(s) 1214
PspFI CCCAGC 1 cut(s) 2697
PspGI CCWGG 3 cut(s) 514, 1679, 2354
PspN4I GGNNCC 6 cut(s) 1054, 1560, 1728, 1729, 2536, 2695
PspPI GGNCC 8 cut(s) 977, 1052, 1193, 1265, 1534, 1559, 1727, 2678
PspPPI RGGWCCY 1 cut(s) 1727
PspXI VCTCGAGB 1 cut(s) 2045
PsrI GAACNNNNNNTAC 2 cut(s) 2862, 2894
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 5 cut(s) 361, 986, 1531, 1609, 2623
PsuI RGATCY 3 cut(s) 754, 1427, 2752
RsaI GTAC 4 cut(s) 624, 1566, 1955, 2402
RsaNI GTAC 4 cut(s) 623, 1565, 1954, 2401
RseI CAYNNNNRTG 3 cut(s) 35, 2369, 2945
SacI GAGCTC 1 cut(s) 1158
SalI GTCGAC 1 cut(s) 2299
SatI GCNGC 6 cut(s) 95, 938, 1480, 1542, 1610, 2630
Sau96I GGNCC 8 cut(s) 977, 1052, 1193, 1265, 1534, 1559, 1727, 2678
SchI GAGTC 2 cut(s) 1430, 2098
ScrFI CCNGG 3 cut(s) 516, 1681, 2356
SduI GDGCHC 1 cut(s) 1158
SfaNI GCATC 5 cut(s) 551, 881, 1116, 1596, 1761
SfcI CTRYAG 3 cut(s) 131, 1721, 1901
Sfr274I CTCGAG 1 cut(s) 2045
SinI GGWCC 6 cut(s) 977, 1052, 1265, 1534, 1727, 2678
SlaI CTCGAG 1 cut(s) 2045
SmiMI CAYNNNNRTG 3 cut(s) 35, 2369, 2945
SmlI CTYRAG 9 cut(s) 932, 1310, 1361, 1650, 1909, 1973, 2045, 2443, 2489
SmoI CTYRAG 9 cut(s) 932, 1310, 1361, 1650, 1909, 1973, 2045, 2443, 2489
SpeI ACTAGT 3 cut(s) 326, 559, 1228
SphI GCATGC 1 cut(s) 1867
SsiI CCGC 2 cut(s) 1335, 2846
SspI AATATT 1 cut(s) 1760
SstI GAGCTC 1 cut(s) 1158
StyD4I CCNGG 3 cut(s) 514, 1679, 2354
StyI CCWWGG 4 cut(s) 422, 1171, 2018, 2149
TaaI ACNGT 5 cut(s) 135, 489, 1875, 2036, 2177
TaiI ACGT 2 cut(s) 1696, 2514
TaqI TCGA 8 cut(s) 249, 848, 1102, 1755, 2046, 2300, 2712, 2922
TatI WGTACW 2 cut(s) 1953, 2400
TfiI GAWTC 9 cut(s) 629, 863, 910, 1509, 2413, 2467, 2485, 2573, 2611
TscAI CASTG 9 cut(s) 492, 892, 1423, 1532, 1880, 2083, 2293, 2536, 2571
TseFI GTSAC 4 cut(s) 1023, 1375, 1892, 1991
TseI GCWGC 6 cut(s) 94, 937, 1479, 1541, 1609, 2629
Tsp45I GTSAC 4 cut(s) 1023, 1375, 1892, 1991
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 9 cut(s) 492, 892, 1423, 1532, 1880, 2083, 2293, 2536, 2571
Vha464I CTTAAG 3 cut(s) 932, 1909, 2489
VpaK11BI GGWCC 6 cut(s) 977, 1052, 1265, 1534, 1727, 2678
VspI ATTAAT 1 cut(s) 2322
XceI RCATGY 1 cut(s) 1867
XcmI CCANNNNNNNNNTGG 2 cut(s) 758, 1569
XhoI CTCGAG 1 cut(s) 2045
XmiI GTMKAC 1 cut(s) 2300
XmnI GAANNNNTTC 1 cut(s) 2779
Zsp2I ATGCAT 2 cut(s) 544, 1754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.