FvH4_7g18910

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007939
Physical Location & Seq
Forward (+)
15734718 .. 15740888
6171 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18910.t1

Sequence Viewer

Length: 3057 bp
ATGGCTACTGGGGGAAAGATAGATACGCTATCCACCCTAGATAATAATGTGCAAGGTTCCAATGGGAATTTATCAGATGTGTTGCAAAGTGAGCTTGAGCTGATTCTAAGGCAGCATCGCAATCAGCAAGCCATTCATATGGGTAGGGATTTGAATATGTATAGGAGTGGCAGTGCACCGCCAACTGTTGAGGGATCGCTGAGTGCTGTGGGTAGCCTGTTTAGGAATTCTGATTTTAGGGATAGGGATATGAGTAGTAGGGTCAGTAGTAACAATGGGGGGTTTTCTGAAGATGAGGTTCGCTCACATATTGCTTATCTCTCGTACTATTATTCACATGATAATATCAACCCAAGAAATCCTCCCCCTTTGTTGTCAAGAGAGGATTGGCGTGTGGCACAGAGGTTTCAGAGTGGATCTTCTTTTGAAGGCAATGGGGATTGGAGGAGGAACAATATAGTTGGCGATGGCGATAAATCGTTGTTTTCAACGCAGCCGGGGCTTTCTGCACAGAAAACAGAAAATGACTTGAACAAATTGAGAAATGCCAATGGGAATAGTATGCCTAGACATACGTCATCTGAGTGGCTTGATAACAGTTCGACTAGTTTGACTGGATTTTCGACAAATGGACTTGGTGCAAGGAGGAAGAGTTTTGCTGACATTCTACAGGAAGGAATTGATCAGCCAACTTCATGCCCCTTGTTAAGACCATCAAGCTGTAATGCTTTTGGTGATGTTATGAATTCAAAAGGCATGGCTGATCCACAACCAGTAGGATTGTGTAATGGAGTAGATTCTATAGAGGGGTTGCACACCGGAGCAGATTTACGTGGCCTGATAGGAATGCAGAACCATGGTACCCCTGTTTCTCAGTCATTTGTATCTGCTGGAGGTCATTCTTTATCAAGAAGTAGAACTCCAGACCCACAATTATTTGGAAGTTCTGGTCTTCCTCCTATGGGAAGAGTGTATCCTAATGTTGGTGGTGCAGATATCCAAAGTGATAATTCGCCTGCCATGACTGAATTGCCCAATATGACAAAAAATTTCTCTGGCTTAAGCCTCTCAAAAGCTACATCTGCAGATGAAGATAATCGTCTTCAGTCTCAGCTTCGCAGGGACCTTGATAATCAGCGTGATTTTCTGTTCAACACACCAAACAGTCAGAACCAGAGGCTGCAACAGCAATTGATAGAGAAGTCAAATGCTGAAAACTTTTCCCTTTCTACAAATTATGCTCATTTAGCAAATAAAAATGGGATTCTGACAAACCGTAATGCACCAAATGGGCATGTTAATTTTCCTAGAAGAACTTCTTCTCCTAGTCTTTATTCAAACGGGAGTTCTTCAGGACTTGGAACTTTTGAAGGGTTGAATGCTCAATACCAGGATGTAAATACCCCAGCAGGAATCGACCTTAATGGTCATGCTTCGGGGGCCTATCCTGCAAATCAGAAGTTGAACAATCATCTTGATGCAGGGTTTACTGGTAATGGTGATGGACAAAGTATGAACAGATTAGGGAACAAATTTGGTCCTGGCCTTCATTCTTCATTTTTAGACCCGTCGCAAATTCAATTATTGCAAACAGCTGATAATTGTGCAAGACGTGCTCTAGCTAGTCCAAGTGGTTATTCTTTGAGTAAAAATAATTTTGGCACTTTGCATGGAGAACTAGAGGGGCAGCGAGCATACCTCGAAGCAATACTTGCTCAAAGGAAGTATGAGATGTCCCTTTTACGTAAATCTGCAGGGTTTAATCATTGTTATATTGGGAATCCATCATATGGTCTTGGAATGACATATCCAGGAATCTCAGTGGCAAACTCATTTCTTCCTTCTGTTGGATCTGGAAGTTCAATGTTTCAGAATGAGAAAATTTCCCGGTTTAATTCTATGATGAAAAGTCCAATGGGAGGATCAGTCGGCGCCTGGAACTCTGATGTTGGAAACGACATAGAAGGAAGACATGCATCATCATCTTCATTACTAGATGAGTTCAAGAACAACAAGAACAAGTCTTTTGAACTTGCAGATATTGTTGATCATGTAGTTGAGTTCAGTACCGATCAGTATGGAAGTCGCTTTATTCAGCAGAAACTAGAAACTGCCACTGTAGAAGAAAAAACAAGAATATTCCCTGAGATTATCCCTCATGCTCGCACCTTGATGACTGATGTGTTTGGAAATTATGTCATACAGAAATTCTTTGAGCATGGTACAGAAAGCCAAAGAAAAGAATTGACGAGCCAACTTACTGGGCATGTTTTGCCTCTCAGTCTGCAAATGTATGGCTGTAGAGTGATCCAGAAGGCGTTGGAAGTGGTTGACATGGATCAGCAGGCTCAAATGGTGTCAGAACTCGATGGTTCAGTCATGAAATGTATCCGTGATCAGAATGGGAATCATGTCATTCAGAAGTGTATAGAATGTGTCCCTCAAGATCAAATTCAGTTTATCATTTCATCCTTCTATGGGCAAGTTGTCACACTGTCCACCCATCCTTATGGTTGCCGTGTCGTGCAGAGGGTTCTGGAACATTGTGATGACTCAAAGACGCAAGAAACTATCATGGATGAAGTTATGCAATCTGTATGCTTTTTGGCCCAGGATCAGTATGGAAATTATGTTATTCAGCATGTCCTTGAACATGGTAAACCGCATGAACGGTCTGCAATTATTCACCAGCTTGCTGGACAAATTGTAAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTCGTGGAGAAATGCTTGATTTTTGGGAGTCCTGAGGAGCGCCAGTTCTTGGTAAAAGAGATGCTTGGTTCCACTGATGAAAATGAGCCGTTGCAGGCCATGATGAAAGATCCATTTGGGAACTATGTGGTGCAGAAGGTTCTTGAGACCTGTGATGATCAGAGTCTTGAGTTGATTGTTTCTCGCATTAAGGTTCATTTGACTGCCTTGAAGAAGTACACTTATGGTAAACATATTGTTACCCGTGTCGAGAAACTCATTACAACGGGAGAAAGGCGCATAGGGTTGTCGTCCTCAGTTTCATCTTCCACAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1019

Amino Acids

111.93

Weight (kDa)

6.25

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 315 - 679 4.3e-88 Nucleic acid binding protein NABP
PUF PF00806 684 - 718 8.6e-12 Pumilio-family RNA binding repeat
PUF PF00806 722 - 751 2.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 756 - 789 1e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 790 - 864 2.7e-06 NOP9-like PUF repeat domain
PUF PF00806 799 - 823 2.6e-06 Pumilio-family RNA binding repeat
PUF PF00806 828 - 863 1.7e-12 Pumilio-family RNA binding repeat
PUF PF00806 866 - 898 3.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 901 - 935 4.2e-08 Pumilio-family RNA binding repeat
PUF PF00806 947 - 976 4.5e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 860
AccB1I GGYRCC 2 cut(s) 860, 1931
AccB7I CCANNNNNTGG 2 cut(s) 1790, 2788
AciI CCGC 2 cut(s) 179, 2663
AclWI GGATC 9 cut(s) 202, 424, 758, 1858, 1930, 2302, 2346, 2622, 2843
AcsI RAATTY 9 cut(s) 67, 226, 745, 1048, 1532, 1575, 1881, 2207, 2451
AcuI CTGAAG 3 cut(s) 309, 1088, 1335
AcyI GRCGYC 1 cut(s) 1932
AfaI GTAC 5 cut(s) 326, 862, 2068, 2224, 2957
AfiI CCNNNNNNNGG 7 cut(s) 962, 983, 1790, 1847, 1919, 2478, 2788
AflII CTTAAG 1 cut(s) 1060
AhlI ACTAGT 1 cut(s) 605
AjiI CACGTC 1 cut(s) 1613
AjnI CCWGG 5 cut(s) 1389, 1540, 1810, 1934, 2610
AjuI GAANNNNNNNTTGG 2 cut(s) 1621, 1653
AloI GAACNNNNNNTCC 4 cut(s) 1306, 1338, 2768, 2800
AluBI AGCT 8 cut(s) 94, 100, 720, 1076, 1114, 1595, 1622, 2692
AluI AGCT 8 cut(s) 94, 100, 720, 1076, 1114, 1595, 1622, 2692
Alw21I GWGCWC 2 cut(s) 178, 1618
Alw26I GTCTC 2 cut(s) 1113, 2879
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 9 cut(s) 202, 424, 758, 1858, 1930, 2302, 2346, 2622, 2843
AlwNI CAGNNNCTG 1 cut(s) 1180
AoxI GGCC 5 cut(s) 835, 1440, 1543, 2607, 2835
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 4 cut(s) 112, 493, 1180, 1687
ApoI RAATTY 9 cut(s) 67, 226, 745, 1048, 1532, 1575, 1881, 2207, 2451
ArsI GACNNNNNNTTYG 2 cut(s) 604, 636
Asp700I GAANNNNTTC 2 cut(s) 1315, 1318
Asp718I GGTACC 1 cut(s) 860
AspLEI GCGC 3 cut(s) 1934, 2781, 3018
AspS9I GGNCC 4 cut(s) 1123, 1440, 1538, 2608
AsuC2I CCSGG 2 cut(s) 498, 1888
AsuHPI GGTGA 3 cut(s) 746, 1511, 2678
AvaII GGWCC 2 cut(s) 1123, 1538
AxyI CCTNAGG 1 cut(s) 2772
BaeGI GKGCMC 1 cut(s) 178
BaeI ACNNNNGTAYC 4 cut(s) 844, 852, 877, 885
BanI GGYRCC 2 cut(s) 860, 1931
BbsI GAAGAC 3 cut(s) 944, 1094, 1975
Bbv12I GWGCWC 2 cut(s) 178, 1618
BbvI GCAGC 4 cut(s) 124, 505, 1167, 1699
BccI CCATC 6 cut(s) 461, 721, 1496, 1792, 2363, 2511
BceAI ACGGC 2 cut(s) 2502, 2812
BciT130I CCWGG 5 cut(s) 1391, 1542, 1812, 1936, 2612
BciVI GTATCC 2 cut(s) 984, 2399
BclI TGATCA 4 cut(s) 682, 2047, 2395, 2896
BcnI CCSGG 2 cut(s) 498, 1888
BcoDI GTCTC 2 cut(s) 1113, 2879
BcuI ACTAGT 1 cut(s) 605
BfmI CTRYAG 6 cut(s) 668, 801, 1083, 1752, 2118, 2299
BfoI RGCGCY 2 cut(s) 1935, 2782
BfrI CTTAAG 1 cut(s) 1060
BfuI GTATCC 2 cut(s) 984, 2399
BisI GCNGC 4 cut(s) 113, 494, 1181, 1688
BlsI GCNGC 4 cut(s) 114, 495, 1182, 1689
Bme1390I CCNGG 7 cut(s) 498, 1391, 1542, 1812, 1888, 1936, 2612
Bme18I GGWCC 2 cut(s) 1123, 1538
BmgBI CACGTC 1 cut(s) 1613
BmgT120I GGNCC 4 cut(s) 1123, 1440, 1538, 2608
BmiI GGNNCC 6 cut(s) 58, 862, 1124, 1441, 1933, 2809
BmrFI CCNGG 7 cut(s) 498, 1391, 1542, 1812, 1888, 1936, 2612
BmrI ACTGGG 2 cut(s) 18, 2271
BmsI GCATC 4 cut(s) 124, 1468, 1985, 2790
BmuI ACTGGG 2 cut(s) 18, 2271
BoxI GACNNNNGTC 1 cut(s) 574
BpiI GAAGAC 3 cut(s) 944, 1094, 1975
BplI GAGNNNNNCTC 4 cut(s) 287, 319, 1683, 1715
BpmI CTGGAG 2 cut(s) 906, 912
BpuEI CTTGAG 4 cut(s) 116, 2427, 2903, 2927
BpuMI CCSGG 2 cut(s) 498, 1888
BsaAI YACGTR 2 cut(s) 833, 1745
BsaHI GRCGYC 1 cut(s) 1932
BsaI GGTCTC 1 cut(s) 2879
BsaJI CCNNGG 3 cut(s) 497, 856, 2610
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 7 cut(s) 962, 983, 1790, 1847, 1919, 2478, 2788
Bse1I ACTGG 6 cut(s) 13, 619, 773, 1495, 2266, 2782
Bse21I CCTNAGG 1 cut(s) 2772
Bse3DI GCAATG 1 cut(s) 439
BseBI CCWGG 5 cut(s) 1391, 1542, 1812, 1936, 2612
BseDI CCNNGG 3 cut(s) 497, 856, 2610
BseGI GGATG 4 cut(s) 1399, 2468, 2503, 2584
BseLI CCNNNNNNNGG 7 cut(s) 962, 983, 1790, 1847, 1919, 2478, 2788
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 9 cut(s) 191, 573, 887, 1124, 1833, 2136, 2293, 2763, 3048
BseNI ACTGG 6 cut(s) 13, 619, 773, 1495, 2266, 2782
BseRI GAGGAG 2 cut(s) 460, 2789
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 4 cut(s) 124, 505, 1167, 1699
BseYI CCCAGC 1 cut(s) 1405
BsgI GTGCAG 4 cut(s) 492, 1011, 2546, 2891
BshFI GGCC 5 cut(s) 837, 1442, 1545, 2609, 2837
BshNI GGYRCC 2 cut(s) 860, 1931
BsiHKAI GWGCWC 2 cut(s) 178, 1618
BsiSI CCGG 3 cut(s) 497, 819, 1888
BslFI GGGAC 3 cut(s) 1136, 1720, 2423
BslI CCNNNNNNNGG 7 cut(s) 962, 983, 1790, 1847, 1919, 2478, 2788
BsmAI GTCTC 2 cut(s) 1113, 2879
BsmFI GGGAC 3 cut(s) 1136, 1720, 2423
BsmI GAATGC 2 cut(s) 852, 1384
BsnI GGCC 5 cut(s) 837, 1442, 1545, 2609, 2837
Bso31I GGTCTC 1 cut(s) 2879
Bsp1286I GDGCHC 2 cut(s) 178, 1618
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 2 cut(s) 179, 2663
BspANI GGCC 5 cut(s) 837, 1442, 1545, 2609, 2837
BspCNI CTCAG 9 cut(s) 192, 574, 886, 1123, 1832, 2137, 2292, 2764, 3047
BspHI TCATGA 1 cut(s) 2379
BspLI GGNNCC 6 cut(s) 58, 862, 1124, 1441, 1933, 2809
BspMAI CTGCAG 2 cut(s) 1087, 1756
BspPI GGATC 9 cut(s) 202, 424, 758, 1858, 1930, 2302, 2346, 2622, 2843
BspT107I GGYRCC 2 cut(s) 860, 1931
BspTI CTTAAG 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 2879
BsrDI GCAATG 1 cut(s) 439
BsrI ACTGG 6 cut(s) 13, 619, 773, 1495, 2266, 2782
BssECI CCNNGG 3 cut(s) 497, 856, 2610
BssNI GRCGYC 1 cut(s) 1932
BssT1I CCWWGG 1 cut(s) 856
Bst2UI CCWGG 5 cut(s) 1391, 1542, 1812, 1936, 2612
Bst4CI ACNGT 7 cut(s) 187, 599, 1166, 1277, 2119, 2496, 2673
Bst6I CTCTTC 2 cut(s) 644, 961
BstACI GRCGYC 1 cut(s) 1932
BstAFI CTTAAG 1 cut(s) 1060
BstAPI GCANNNNNTGC 3 cut(s) 1613, 1712, 2272
BstBAI YACGTR 2 cut(s) 833, 1745
BstC8I GCNNGC 7 cut(s) 129, 1017, 1692, 2164, 2346, 2694, 2835
BstDSI CCRYGG 1 cut(s) 856
BstF5I GGATG 4 cut(s) 1399, 2468, 2503, 2584
BstH2I RGCGCY 2 cut(s) 1935, 2782
BstHHI GCGC 3 cut(s) 1934, 2781, 3018
BstMAI GTCTC 2 cut(s) 1113, 2879
BstNI CCWGG 5 cut(s) 1391, 1542, 1812, 1936, 2612
BstNSI RCATGY 4 cut(s) 1298, 1976, 2270, 2645
BstPAI GACNNNNGTC 1 cut(s) 574
BstSCI CCNGG 7 cut(s) 496, 1389, 1540, 1810, 1886, 1934, 2610
BstSFI CTRYAG 6 cut(s) 668, 801, 1083, 1752, 2118, 2299
BstSLI GKGCMC 1 cut(s) 178
BstSNI TACGTA 1 cut(s) 1745
BstV1I GCAGC 4 cut(s) 124, 505, 1167, 1699
BstV2I GAAGAC 3 cut(s) 944, 1094, 1975
BstX2I RGATCY 3 cut(s) 416, 1850, 2848
BstXI CCANNNNNNTGG 4 cut(s) 139, 2261, 2510, 2696
BstYI RGATCY 3 cut(s) 416, 1850, 2848
Bsu36I CCTNAGG 1 cut(s) 2772
BsuI GTATCC 2 cut(s) 984, 2399
BsuRI GGCC 5 cut(s) 837, 1442, 1545, 2609, 2837
BtgI CCRYGG 1 cut(s) 856
BtgZI GCGATG 2 cut(s) 101, 480
BtrI CACGTC 1 cut(s) 1613
BtsCI GGATG 4 cut(s) 1399, 2468, 2503, 2584
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 5 cut(s) 178, 1827, 2115, 2492, 2811
Cac8I GCNNGC 7 cut(s) 129, 1017, 1692, 2164, 2346, 2694, 2835
CaiI CAGNNNCTG 1 cut(s) 1180
CciI TCATGA 1 cut(s) 2379
CfoI GCGC 3 cut(s) 1934, 2781, 3018
Cfr13I GGNCC 4 cut(s) 1123, 1440, 1538, 2608
CseI GACGC 1 cut(s) 2569
Csp6I GTAC 5 cut(s) 325, 861, 2067, 2223, 2956
CviQI GTAC 5 cut(s) 325, 861, 2067, 2223, 2956
DinI GGCGCC 1 cut(s) 1933
Eam1104I CTCTTC 2 cut(s) 644, 961
EarI CTCTTC 2 cut(s) 644, 961
Eco105I TACGTA 1 cut(s) 1745
Eco130I CCWWGG 1 cut(s) 856
Eco31I GGTCTC 1 cut(s) 2879
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 2 cut(s) 1123, 1538
Eco57I CTGAAG 3 cut(s) 309, 1088, 1335
Eco81I CCTNAGG 1 cut(s) 2772
EcoO109I RGGNCCY 2 cut(s) 1123, 1440
EcoRI GAATTC 2 cut(s) 226, 745
EcoRII CCWGG 5 cut(s) 1389, 1540, 1810, 1934, 2610
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 1 cut(s) 1978
EgeI GGCGCC 1 cut(s) 1933
EheI GGCGCC 1 cut(s) 1933
ErhI CCWWGG 1 cut(s) 856
FalI AAGNNNNNCTT 6 cut(s) 1303, 1335, 1695, 1727, 2787, 2819
FaqI GGGAC 3 cut(s) 1136, 1720, 2423
FauNDI CATATG 2 cut(s) 138, 1789
FbaI TGATCA 4 cut(s) 682, 2047, 2395, 2896
Fnu4HI GCNGC 4 cut(s) 113, 494, 1181, 1688
FokI GGATG 4 cut(s) 1406, 2455, 2490, 2591
Fsp4HI GCNGC 4 cut(s) 113, 494, 1181, 1688
GlaI GCGC 3 cut(s) 1933, 2780, 3017
GluI GCNGC 4 cut(s) 113, 494, 1181, 1688
GsaI CCCAGC 1 cut(s) 1409
GsuI CTGGAG 2 cut(s) 906, 912
HaeII RGCGCY 2 cut(s) 1935, 2782
HaeIII GGCC 5 cut(s) 837, 1442, 1545, 2609, 2837
HapII CCGG 3 cut(s) 497, 819, 1888
HgaI GACGC 1 cut(s) 2569
HhaI GCGC 3 cut(s) 1934, 2781, 3018
Hin1I GRCGYC 1 cut(s) 1932
Hin6I GCGC 3 cut(s) 1932, 2779, 3016
HinP1I GCGC 3 cut(s) 1932, 2779, 3016
HincII GTYRAC 1 cut(s) 2332
HindII GTYRAC 1 cut(s) 2332
HpaII CCGG 3 cut(s) 497, 819, 1888
HphI GGTGA 3 cut(s) 746, 1511, 2678
Hpy166II GTNNAC 7 cut(s) 176, 1488, 2332, 2499, 2660, 2958, 2969
Hpy8I GTNNAC 7 cut(s) 176, 1488, 2332, 2499, 2660, 2958, 2969
Hpy99I CGWCG 1 cut(s) 1573
HpyAV CCTTC 9 cut(s) 422, 668, 1364, 1556, 1851, 1958, 2308, 2482, 2869
HpyCH4III ACNGT 7 cut(s) 187, 599, 1166, 1277, 2119, 2496, 2673
HpyCH4IV ACGT 4 cut(s) 575, 832, 1612, 1744
HpySE526I ACGT 4 cut(s) 575, 832, 1612, 1744
Hsp92I GRCGYC 1 cut(s) 1932
HspAI GCGC 3 cut(s) 1932, 2779, 3016
KasI GGCGCC 1 cut(s) 1931
KpnI GGTACC 1 cut(s) 864
Ksp22I TGATCA 4 cut(s) 682, 2047, 2395, 2896
LmnI GCTCC 2 cut(s) 821, 2776
Lsp1109I GCAGC 4 cut(s) 124, 505, 1167, 1699
LweI GCATC 4 cut(s) 124, 1468, 1985, 2790
MaeII ACGT 4 cut(s) 575, 832, 1612, 1744
MaeIII GTNAC 3 cut(s) 269, 2488, 2977
MfeI CAATTG 1 cut(s) 1190
MflI RGATCY 3 cut(s) 416, 1850, 2848
MhlI GDGCHC 2 cut(s) 178, 1618
Mly113I GGCGCC 1 cut(s) 1932
MlyI GAGTC 4 cut(s) 2546, 2725, 2776, 2911
MmeI TCCRAC 3 cut(s) 1828, 1930, 2301
Mph1103I ATGCAT 1 cut(s) 1978
MroXI GAANNNNTTC 2 cut(s) 1315, 1318
MseI TTAA 7 cut(s) 707, 1061, 1299, 1422, 1761, 1893, 2928
MslI CAYNNNNRTG 6 cut(s) 137, 583, 1476, 2171, 2508, 2547
MspA1I CMGCKG 1 cut(s) 1595
MspCI CTTAAG 1 cut(s) 1060
MspI CCGG 3 cut(s) 497, 819, 1888
MspR9I CCNGG 7 cut(s) 498, 1391, 1542, 1812, 1888, 1936, 2612
MunI CAATTG 1 cut(s) 1190
Mva1269I GAATGC 2 cut(s) 852, 1384
MvaI CCWGG 5 cut(s) 1391, 1542, 1812, 1936, 2612
NarI GGCGCC 1 cut(s) 1932
NciI CCSGG 2 cut(s) 498, 1888
NcoI CCATGG 1 cut(s) 856
NdeI CATATG 2 cut(s) 138, 1789
NlaIV GGNNCC 6 cut(s) 58, 862, 1124, 1441, 1933, 2809
NmuCI GTSAC 1 cut(s) 2488
NsiI ATGCAT 1 cut(s) 1978
NspI RCATGY 4 cut(s) 1298, 1976, 2270, 2645
PagI TCATGA 1 cut(s) 2379
PctI GAATGC 2 cut(s) 852, 1384
PdmI GAANNNNTTC 2 cut(s) 1315, 1318
PfeI GAWTC 7 cut(s) 103, 797, 1264, 1413, 1780, 1815, 2407
PflMI CCANNNNNTGG 2 cut(s) 1790, 2788
PfoI TCCNGGA 1 cut(s) 1810
PkrI GCNGC 4 cut(s) 114, 495, 1182, 1689
PleI GAGTC 4 cut(s) 2546, 2724, 2775, 2910
PluTI GGCGCC 1 cut(s) 1935
PpsI GAGTC 4 cut(s) 2546, 2724, 2775, 2910
Ppu21I YACGTR 2 cut(s) 833, 1745
PpuMI RGGWCCY 1 cut(s) 1123
PshAI GACNNNNGTC 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 1123
Psp6I CCWGG 5 cut(s) 1389, 1540, 1810, 1934, 2610
PspFI CCCAGC 1 cut(s) 1405
PspGI CCWGG 5 cut(s) 1389, 1540, 1810, 1934, 2610
PspN4I GGNNCC 6 cut(s) 58, 862, 1124, 1441, 1933, 2809
PspPI GGNCC 4 cut(s) 1123, 1440, 1538, 2608
PspPPI RGGWCCY 1 cut(s) 1123
PstI CTGCAG 2 cut(s) 1087, 1756
PstNI CAGNNNCTG 1 cut(s) 1180
PsuI RGATCY 3 cut(s) 416, 1850, 2848
PvuII CAGCTG 1 cut(s) 1595
RsaI GTAC 5 cut(s) 326, 862, 2068, 2224, 2957
RsaNI GTAC 5 cut(s) 325, 861, 2067, 2223, 2956
RseI CAYNNNNRTG 6 cut(s) 137, 583, 1476, 2171, 2508, 2547
SaqAI TTAA 7 cut(s) 707, 1061, 1299, 1422, 1761, 1893, 2928
SatI GCNGC 4 cut(s) 113, 494, 1181, 1688
Sau96I GGNCC 4 cut(s) 1123, 1440, 1538, 2608
SchI GAGTC 4 cut(s) 2546, 2725, 2776, 2911
ScrFI CCNGG 7 cut(s) 498, 1391, 1542, 1812, 1888, 1936, 2612
SduI GDGCHC 2 cut(s) 178, 1618
SfaNI GCATC 4 cut(s) 124, 1468, 1985, 2790
SfcI CTRYAG 6 cut(s) 668, 801, 1083, 1752, 2118, 2299
SfoI GGCGCC 1 cut(s) 1933
SinI GGWCC 2 cut(s) 1123, 1538
SmiMI CAYNNNNRTG 6 cut(s) 137, 583, 1476, 2171, 2508, 2547
SmlI CTYRAG 5 cut(s) 95, 1060, 2442, 2882, 2906
SmoI CTYRAG 5 cut(s) 95, 1060, 2442, 2882, 2906
SnaBI TACGTA 1 cut(s) 1745
SpeI ACTAGT 1 cut(s) 605
SsiI CCGC 2 cut(s) 179, 2663
SspDI GGCGCC 1 cut(s) 1931
SspI AATATT 1 cut(s) 2139
StyD4I CCNGG 7 cut(s) 496, 1389, 1540, 1810, 1886, 1934, 2610
StyI CCWWGG 1 cut(s) 856
TaaI ACNGT 7 cut(s) 187, 599, 1166, 1277, 2119, 2496, 2673
TaiI ACGT 4 cut(s) 578, 835, 1615, 1747
TaqI TCGA 6 cut(s) 602, 623, 1416, 1701, 2367, 2988
TatI WGTACW 1 cut(s) 2955
TfiI GAWTC 7 cut(s) 103, 797, 1264, 1413, 1780, 1815, 2407
Tru1I TTAA 7 cut(s) 707, 1061, 1299, 1422, 1761, 1893, 2928
Tru9I TTAA 7 cut(s) 707, 1061, 1299, 1422, 1761, 1893, 2928
TscAI CASTG 5 cut(s) 178, 1827, 2122, 2499, 2818
TseFI GTSAC 1 cut(s) 2488
TseI GCWGC 4 cut(s) 112, 493, 1180, 1687
Tsp45I GTSAC 1 cut(s) 2488
TspGWI ACGGA 1 cut(s) 2381
TspRI CASTG 5 cut(s) 178, 1827, 2122, 2499, 2818
Van91I CCANNNNNTGG 2 cut(s) 1790, 2788
Vha464I CTTAAG 1 cut(s) 1060
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 1123, 1538
XapI RAATTY 9 cut(s) 67, 226, 745, 1048, 1532, 1575, 1881, 2207, 2451
XceI RCATGY 4 cut(s) 1298, 1976, 2270, 2645
XcmI CCANNNNNNNNNTGG 1 cut(s) 2618
XmnI GAANNNNTTC 2 cut(s) 1315, 1318
Zsp2I ATGCAT 1 cut(s) 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.