RLG00000027618

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007939
Physical Location & Seq
Reverse (-)
13194925 .. 13199757
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027618

Sequence Viewer

Length: 3069 bp
ATGACTAGTGGGGGAAAGATAGATATGCTATCAACGCTAGATAATAATGTTCGAGGTTCAAATGGGAATTTAGAAGATGCTTTGCAGAGTGAGCTTGAGCTGATTCTAAGGGAGCAGCGCAATCAGCACGCCATTCATCGGGATAGGGAAATGAATATGTATAGGAGTGGCAGTGCACCGCCAACTGTGGAGGGATCGCTGAGTGCTGTGGGTAGTCTGTTTAGGAATTCTGATTTTAGGGATAGGGACATGAGAAGTAGGGTTAGTAGTAACAATGGGGGTTTGTCTGAAGATGAGATTCGCTCACATCCTGCTTACCTTTCGTACTATTATTCACATGATAATATCAACCCAAGATTGCCTCCGCCATTGTTGTCAAGAGAGGATTGGCGTGTTGCACAGAGGTTTCAGAGTGGGTCTTCTTTTGAAGGTGCTGGAGATTGGAGGAAGAACAATTTAGTTGGCGATGGTGATAAGTCGTTGTTTTCAATGCAGCTGGGGCTTTCTGCACAGAAGGCAGATAATGACTTGATCAAATTTAGAAATGCCAATGGGAGTAGTATGCATGGACAGACGTCATCCGAGTGGCTGGACAGAAATTCAAATAGTTTGACTGGATTCCCTACAAATGGACTTGGTGCAAGGAGGAAGAGTTTTGCTGACATTCTACAGGAAGGAAATGATCAACCAGCTTCCTTGTCAGGCCCCCTGTCAAGGCCATCAAGCCGTAATGCTTTTGGTGATGTCATGAATTCAACCGGCATGGCTGATCCACATGCAGTAGGGTTGTGTAATGGAGTGGATTCCATAGAGGGGTTGCACACCGGAGCAGCTCCACGTGGCTTGATAGGAATGCAGAGCCATGGTACCCCAGTTTCTCATTCCTTTGCTGGAGGTCCTTCTCTGTCAAGAAGCAGAACCCCTGAACCACAATTATTTGGAAGTTCTGGTCTTCCTTCTGTGGGAAGAGCGTTTCCTAATGTTGCTGGTGCAGATATCCAAAATGATAGTTCGCCTGCCATGACTGAGTTGCCCAATATGACAACAAATTTGTCTGGCTTAAGCCTCTCAAAAGTTAGATCTGCAGATGAAGATAGTCGTCTTCAGTCTCAGCTTCGTATGGACCTTCATAATCAGTGTGATTTTCTGTTCAACACACCAAACAGTCAGAATCAGAGGCTGCAGCAGCATCTGATAGAGAAGTCAAATGCTGAAAACTTTTCCCTTTCTACAAATTATGCTCATCTAGCAAATAAAAATGGGATTCTAACAAACCGTAATGCATCAAATGGGCATTTTCATTTTCCTAGAAGAACTTCTTCTCCCAGTCTTTACTCAAACGGGAGTCCTTCAGGACTTGGAACTTTGGAAGGGTTGAATGCTCATTACCAGGATGCAAATACCCCAGGAATCGAGTTTGATGGTCATGCTGCTGGGGCATATCCTTTGAATAGTTGGTTACCCCATCAATCTCTTTTTAATTTATTTTTGGGTGCAGCAGGTTTTACTGGTAGTGGTGACGGACAAAGTATGAATAGATTAGGGAACAAAGTAGGGCCTGGCCTTCATTCTTCATTTGTGGACCCATCATATATTCAGTTAATGCAAAGAACTGATTATGCAGCACATGCTCTGGCTAGTCCAAGTGGTTATTCTCTGAGTAAAAATAACTTTGGCAATTTGCATGGAGACTTAGAGGGGCTTCAGAAAGCATACCTCGAGGCAATACTTGCTCAAAGGAAGCAACAGTACGAGTTGTCCCTTTTAAGTCAATCTGGGGGGTTTGATCATGGTTATTTTGGGAATCCATCATATGGCCTGGGGATGACATATCCAGGAATCTCAGTGGCAAACTCTGTTCTTCCTTCTGTTGGATCTGGAAGTTCAATGTTTCAGAATGAGAAGATTTCCCGCTTTAATTCTATTATGAGAAGTTCAGCGGGAGGTTCAGTCAGCACCTGGCAGTCAGATGCTGGAAATGACTTAGAAGGAAGACATGCATCATCATTATTAGATGAGTTCAAGAACAACAAGAATAAGTCTTTTGAACTTGCAGATATTGTTGATCATGTAGTTGAATTCAGTACCGATCAGTATGGAAGTCGCTTTATCCAGCAGAAACTCGAAACTGCCACTGTAGAAGAAAAAATGAAAATATTCCCTGAGATTATTCCTCAAGCTCGCACCTTGATGACTGATGTTTTTGGAAATTATGTCATACAGAAATTCTTTGAGCATGGTACAGAAAGCCAAAGAAACGAATTGACGAACCAACTTGCTGGCCATGTTTTGCCTCTCAGTCTGCAAATGTATGGTTGTAGAGTGATTCAGAAGGCCTTGGAAGTGGTTGACAAGGATCATCAGGCTCAAATGGTGTCAGAACTCGATGGTTCAGTCATGAAATGTGTCCGGGATCAAAATGGGAATCATGTCATTCAGAAGAGTATAGAATGTGTCCCTCAAGATCAAATTCAGTTCATCATTTCATCCTTCTATGGGCAAGTTGTCACACTGTCCACCCATCCTTATGGCTGCCGTGTCATACAGAGGGTTCTGGAACATTGTGATGACTCAAAGACGCAAGAAATTATCATGGATGAAGTCATGCAATCTGTATGCATTCTGGCACAGGATCAGTATGGGAATTATGTTATTCAGCACGTCCTTGAACATGGTAAACCACATGAACGGACTGCAATTATTCACAAGCTTGCTGGACAAATTGTAAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAAAAGTGCTTGTCTTTTGGGAGTGCTGAGGAGCGCCAGTTGTTGGTGAAAGAGATGCTTGGTTCCACTGATGAAAATGAGCCGTTGCAGGCCATGATGAAAGATCCATTTGGAAACTACGTCGTGCAGAAGGTTTTGGAGACCTGTGATGATCAGAGTCTTGAGTTGATTGTTTCTCGCATTAAGGTTCATTTGACTGCCTTGAAGAAGTACACCTATGGTAAACATATCGTTTCCCGTGTTGAAAAACTCATCACAACTGGAGAAAGGCGCATAGGATTGTCAACCTCAGTTTCTTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1023

Amino Acids

112.17

Weight (kDa)

6.17

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 327 - 685 3.6e-102 Nucleic acid binding protein NABP
PUF PF00806 690 - 724 1.7e-11 Pumilio-family RNA binding repeat
PUF PF00806 729 - 758 3.1e-08 Pumilio-family RNA binding repeat
PUF PF00806 762 - 795 2.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 834 - 868 7.8e-13 Pumilio-family RNA binding repeat
PUF PF00806 872 - 904 2.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 907 - 940 8.6e-08 Pumilio-family RNA binding repeat
PUF PF00806 953 - 982 4.5e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 578
AbsI CCTCGAGG 1 cut(s) 1718
Acc36I ACCTGC 1 cut(s) 1490
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 2 cut(s) 1814, 2806
AciI CCGC 4 cut(s) 179, 365, 1912, 1940
AclWI GGATC 7 cut(s) 202, 764, 1882, 2364, 2421, 2640, 2861
AcoI YGGCCR 1 cut(s) 2281
AcsI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1048, 2078, 2225, 2469
AcuI CTGAAG 4 cut(s) 309, 1088, 1335, 1688
AcvI CACGTG 1 cut(s) 839
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 6 cut(s) 326, 868, 1751, 2086, 2242, 2975
AfiI CCNNNNNNNGG 9 cut(s) 138, 629, 714, 813, 962, 1814, 1871, 2496, 2806
AflII CTTAAG 1 cut(s) 1060
AhlI ACTAGT 1 cut(s) 5
AjiI CACGTC 1 cut(s) 2662
AjnI CCWGG 6 cut(s) 1389, 1405, 1558, 1818, 1834, 1958
AjuI GAANNNNNNNTTGG 2 cut(s) 1636, 1668
AloI GAACNNNNNNTCC 2 cut(s) 1306, 1338
AluBI AGCT 8 cut(s) 94, 100, 496, 692, 833, 1114, 2180, 2710
AluI AGCT 8 cut(s) 94, 100, 496, 692, 833, 1114, 2180, 2710
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 3 cut(s) 1113, 1683, 2897
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 7 cut(s) 202, 764, 1882, 2364, 2421, 2640, 2861
AlwNI CAGNNNCTG 4 cut(s) 1180, 1192, 1959, 1973
Ama87I CYCGRG 1 cut(s) 1718
AoxI GGCC 8 cut(s) 703, 716, 1556, 1561, 1816, 2281, 2334, 2853
ApaLI GTGCAC 1 cut(s) 174
ApoI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1048, 2078, 2225, 2469
ArsI GACNNNNNNTTYG 2 cut(s) 1033, 1065
Asp700I GAANNNNTTC 3 cut(s) 1315, 1318, 2156
Asp718I GGTACC 1 cut(s) 866
AspLEI GCGC 3 cut(s) 120, 2799, 3036
AspS9I GGNCC 5 cut(s) 704, 896, 1123, 1556, 1582
AsuC2I CCSGG 1 cut(s) 2411
AsuHPI GGTGA 4 cut(s) 482, 752, 1529, 2821
AvaI CYCGRG 1 cut(s) 1718
AvaII GGWCC 3 cut(s) 896, 1123, 1582
BaeGI GKGCMC 1 cut(s) 178
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 1 cut(s) 2283
BanI GGYRCC 1 cut(s) 866
BarI GAAGNNNNNNTAC 2 cut(s) 1733, 1765
BbrPI CACGTG 1 cut(s) 839
BbsI GAAGAC 4 cut(s) 411, 944, 1094, 1999
Bbv12I GWGCWC 1 cut(s) 178
BbvCI CCTCAGC 1 cut(s) 2790
BccI CCATC 8 cut(s) 461, 727, 1415, 1473, 1594, 1816, 2381, 2529
BceAI ACGGC 3 cut(s) 711, 2520, 2830
BciT130I CCWGG 6 cut(s) 1391, 1407, 1560, 1820, 1836, 1960
BclI TGATCA 5 cut(s) 531, 682, 1786, 2065, 2914
BcnI CCSGG 1 cut(s) 2411
BcoDI GTCTC 3 cut(s) 1113, 1683, 2897
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 5 cut(s) 6, 38, 1247, 1308, 1638
BfmI CTRYAG 4 cut(s) 668, 1083, 1181, 2136
BfoI RGCGCY 1 cut(s) 2800
BfrI CTTAAG 1 cut(s) 1060
BfuAI ACCTGC 1 cut(s) 1490
BglII AGATCT 1 cut(s) 1079
Bme1390I CCNGG 7 cut(s) 1391, 1407, 1560, 1820, 1836, 1960, 2411
Bme18I GGWCC 3 cut(s) 896, 1123, 1582
BmeT110I CYCGRG 1 cut(s) 1718
BmgBI CACGTC 1 cut(s) 2662
BmgT120I GGNCC 5 cut(s) 704, 896, 1123, 1556, 1582
BmiI GGNNCC 4 cut(s) 706, 868, 1584, 2827
BmrFI CCNGG 7 cut(s) 1391, 1407, 1560, 1820, 1836, 1960, 2411
BmrI ACTGGG 2 cut(s) 866, 1320
BmsI GCATC 7 cut(s) 67, 1198, 1292, 1384, 1960, 2009, 2808
BmuI ACTGGG 2 cut(s) 866, 1320
BoxI GACNNNNGTC 1 cut(s) 574
BpiI GAAGAC 4 cut(s) 411, 944, 1094, 1999
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 3 cut(s) 456, 912, 3045
Bpu10I CCTNAGC 1 cut(s) 2790
BpuEI CTTGAG 4 cut(s) 116, 2160, 2445, 2945
BpuMI CCSGG 1 cut(s) 2411
BsaAI YACGTR 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 2897
BsaJI CCNNGG 4 cut(s) 862, 1405, 1819, 2337
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 9 cut(s) 138, 629, 714, 813, 962, 1814, 1871, 2496, 2806
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 6 cut(s) 619, 872, 1326, 1513, 2800, 3028
BseBI CCWGG 6 cut(s) 1391, 1407, 1560, 1820, 1836, 1960
BseDI CCNNGG 4 cut(s) 862, 1405, 1819, 2337
BseGI GGATG 7 cut(s) 307, 578, 1399, 1830, 2486, 2521, 2602
BseLI CCNNNNNNNGG 9 cut(s) 138, 629, 714, 813, 962, 1814, 1871, 2496, 2806
BseMII CTCAG 9 cut(s) 191, 1017, 1124, 1649, 1857, 2154, 2311, 2781, 3066
BseNI ACTGG 6 cut(s) 619, 872, 1326, 1513, 2800, 3028
BseRI GAGGAG 1 cut(s) 2807
BseSI GKGCMC 1 cut(s) 178
BseYI CCCAGC 2 cut(s) 496, 1433
BsgI GTGCAG 4 cut(s) 492, 1011, 1515, 2909
BshFI GGCC 8 cut(s) 705, 718, 1558, 1563, 1818, 2283, 2336, 2855
BshNI GGYRCC 1 cut(s) 866
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 1718
BsiSI CCGG 3 cut(s) 759, 825, 2410
BslFI GGGAC 3 cut(s) 260, 1744, 2441
BslI CCNNNNNNNGG 9 cut(s) 138, 629, 714, 813, 962, 1814, 1871, 2496, 2806
BsmAI GTCTC 3 cut(s) 1113, 1683, 2897
BsmFI GGGAC 3 cut(s) 260, 1744, 2441
BsmI GAATGC 3 cut(s) 858, 1384, 2619
BsnI GGCC 8 cut(s) 705, 718, 1558, 1563, 1818, 2283, 2336, 2855
Bso31I GGTCTC 1 cut(s) 2897
BsoBI CYCGRG 1 cut(s) 1718
Bsp1286I GDGCHC 1 cut(s) 178
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 4 cut(s) 179, 365, 1912, 1940
BspANI GGCC 8 cut(s) 705, 718, 1558, 1563, 1818, 2283, 2336, 2855
BspCNI CTCAG 9 cut(s) 192, 1018, 1123, 1650, 1856, 2155, 2310, 2782, 3065
BspHI TCATGA 2 cut(s) 747, 2397
BspLI GGNNCC 4 cut(s) 706, 868, 1584, 2827
BspMAI CTGCAG 2 cut(s) 1087, 1185
BspMI ACCTGC 1 cut(s) 1490
BspPI GGATC 7 cut(s) 202, 764, 1882, 2364, 2421, 2640, 2861
BspQI GCTCTTC 1 cut(s) 961
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 2897
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 6 cut(s) 619, 872, 1326, 1513, 2800, 3028
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 4 cut(s) 862, 1405, 1819, 2337
BssNI GRCGYC 1 cut(s) 575
BssT1I CCWWGG 2 cut(s) 862, 2337
Bst2UI CCWGG 6 cut(s) 1391, 1407, 1560, 1820, 1836, 1960
Bst4CI ACNGT 6 cut(s) 187, 1166, 1277, 1749, 2137, 2514
Bst6I CTCTTC 3 cut(s) 644, 961, 2435
BstACI GRCGYC 1 cut(s) 575
BstAFI CTTAAG 1 cut(s) 1060
BstAPI GCANNNNNTGC 2 cut(s) 1628, 1730
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 6 cut(s) 129, 1017, 2182, 2281, 2712, 2853
BstDSI CCRYGG 1 cut(s) 862
BstEII GGTNACC 1 cut(s) 1458
BstF5I GGATG 7 cut(s) 307, 578, 1399, 1830, 2486, 2521, 2602
BstH2I RGCGCY 1 cut(s) 2800
BstHHI GCGC 3 cut(s) 120, 2799, 3036
BstMAI GTCTC 3 cut(s) 1113, 1683, 2897
BstNI CCWGG 6 cut(s) 1391, 1407, 1560, 1820, 1836, 1960
BstNSI RCATGY 3 cut(s) 779, 1631, 2000
BstPAI GACNNNNGTC 1 cut(s) 574
BstPI GGTNACC 1 cut(s) 1458
BstSCI CCNGG 7 cut(s) 1389, 1405, 1558, 1818, 1834, 1958, 2409
BstSFI CTRYAG 4 cut(s) 668, 1083, 1181, 2136
BstSLI GKGCMC 1 cut(s) 178
BstV2I GAAGAC 4 cut(s) 411, 944, 1094, 1999
BstX2I RGATCY 3 cut(s) 1079, 1874, 2866
BstXI CCANNNNNNTGG 2 cut(s) 2279, 2528
BstYI RGATCY 3 cut(s) 1079, 1874, 2866
BsuRI GGCC 8 cut(s) 705, 718, 1558, 1563, 1818, 2283, 2336, 2855
BtgI CCRYGG 1 cut(s) 862
BtgZI GCGATG 1 cut(s) 480
BtrI CACGTC 1 cut(s) 2662
BtsCI GGATG 7 cut(s) 307, 578, 1399, 1830, 2486, 2521, 2602
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 6 cut(s) 178, 1142, 1851, 2133, 2510, 2829
BveI ACCTGC 1 cut(s) 1490
Cac8I GCNNGC 6 cut(s) 129, 1017, 2182, 2281, 2712, 2853
CaiI CAGNNNCTG 4 cut(s) 1180, 1192, 1959, 1973
CciI TCATGA 2 cut(s) 747, 2397
CfoI GCGC 3 cut(s) 120, 2799, 3036
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 5 cut(s) 704, 896, 1123, 1556, 1582
CseI GACGC 1 cut(s) 2587
Csp6I GTAC 6 cut(s) 325, 867, 1750, 2085, 2241, 2974
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviQI GTAC 6 cut(s) 325, 867, 1750, 2085, 2241, 2974
EaeI YGGCCR 1 cut(s) 2281
Eam1104I CTCTTC 3 cut(s) 644, 961, 2435
EarI CTCTTC 3 cut(s) 644, 961, 2435
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 862, 2337
Eco147I AGGCCT 1 cut(s) 2336
Eco31I GGTCTC 1 cut(s) 2897
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 3 cut(s) 896, 1123, 1582
Eco57I CTGAAG 4 cut(s) 309, 1088, 1335, 1688
Eco72I CACGTG 1 cut(s) 839
Eco88I CYCGRG 1 cut(s) 1718
Eco91I GGTNACC 1 cut(s) 1458
EcoO109I RGGNCCY 3 cut(s) 704, 896, 1556
EcoO65I GGTNACC 1 cut(s) 1458
EcoRI GAATTC 3 cut(s) 226, 751, 2078
EcoRII CCWGG 6 cut(s) 1389, 1405, 1558, 1818, 1834, 1958
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 2 cut(s) 862, 2337
EcoT22I ATGCAT 4 cut(s) 567, 1285, 2002, 2621
ErhI CCWWGG 2 cut(s) 862, 2337
FalI AAGNNNNNCTT 4 cut(s) 1303, 1335, 2805, 2837
FaqI GGGAC 3 cut(s) 260, 1744, 2441
FauI CCCGC 2 cut(s) 1919, 1933
FauNDI CATATG 1 cut(s) 1813
FbaI TGATCA 5 cut(s) 531, 682, 1786, 2065, 2914
FokI GGATG 7 cut(s) 294, 565, 1406, 1837, 2473, 2508, 2609
FspBI CTAG 5 cut(s) 6, 38, 1247, 1308, 1638
GlaI GCGC 3 cut(s) 119, 2798, 3035
GsaI CCCAGC 2 cut(s) 500, 1437
GsuI CTGGAG 3 cut(s) 456, 912, 3045
HaeII RGCGCY 1 cut(s) 2800
HaeIII GGCC 8 cut(s) 705, 718, 1558, 1563, 1818, 2283, 2336, 2855
HapII CCGG 3 cut(s) 759, 825, 2410
HgaI GACGC 1 cut(s) 2587
HhaI GCGC 3 cut(s) 120, 2799, 3036
Hin1I GRCGYC 1 cut(s) 575
Hin6I GCGC 3 cut(s) 118, 2797, 3034
HinP1I GCGC 3 cut(s) 118, 2797, 3034
HincII GTYRAC 2 cut(s) 2350, 3048
HindII GTYRAC 2 cut(s) 2350, 3048
HindIII AAGCTT 1 cut(s) 2708
HpaII CCGG 3 cut(s) 759, 825, 2410
HphI GGTGA 4 cut(s) 482, 752, 1529, 2821
Hpy166II GTNNAC 8 cut(s) 176, 1582, 2350, 2517, 2678, 2976, 2987, 3048
Hpy8I GTNNAC 8 cut(s) 176, 1582, 2350, 2517, 2678, 2976, 2987, 3048
Hpy99I CGWCG 1 cut(s) 2888
HpyCH4III ACNGT 6 cut(s) 187, 1166, 1277, 1749, 2137, 2514
HpyCH4IV ACGT 4 cut(s) 575, 838, 2661, 2883
HpySE526I ACGT 4 cut(s) 575, 838, 2661, 2883
Hsp92I GRCGYC 1 cut(s) 575
HspAI GCGC 3 cut(s) 118, 2797, 3034
KpnI GGTACC 1 cut(s) 870
Ksp22I TGATCA 5 cut(s) 531, 682, 1786, 2065, 2914
LguI GCTCTTC 1 cut(s) 961
LmnI GCTCC 4 cut(s) 112, 827, 838, 2794
LweI GCATC 7 cut(s) 67, 1198, 1292, 1384, 1960, 2009, 2808
MaeI CTAG 5 cut(s) 6, 38, 1247, 1308, 1638
MaeII ACGT 4 cut(s) 575, 838, 2661, 2883
MaeIII GTNAC 4 cut(s) 269, 1458, 1517, 2506
MflI RGATCY 3 cut(s) 1079, 1874, 2866
MhlI GDGCHC 1 cut(s) 178
MlsI TGGCCA 1 cut(s) 2283
MluNI TGGCCA 1 cut(s) 2283
MlyI GAGTC 4 cut(s) 1354, 2564, 2743, 2929
MmeI TCCRAC 1 cut(s) 1852
Mox20I TGGCCA 1 cut(s) 2283
Mph1103I ATGCAT 4 cut(s) 567, 1285, 2002, 2621
MroXI GAANNNNTTC 3 cut(s) 1315, 1318, 2156
MscI TGGCCA 1 cut(s) 2283
MseI TTAA 6 cut(s) 1061, 1479, 1601, 1766, 1917, 2946
MslI CAYNNNNRTG 4 cut(s) 583, 2189, 2526, 2565
Msp20I TGGCCA 1 cut(s) 2283
MspA1I CMGCKG 2 cut(s) 496, 1940
MspCI CTTAAG 1 cut(s) 1060
MspI CCGG 3 cut(s) 759, 825, 2410
MspR9I CCNGG 7 cut(s) 1391, 1407, 1560, 1820, 1836, 1960, 2411
Mva1269I GAATGC 3 cut(s) 858, 1384, 2619
MvaI CCWGG 6 cut(s) 1391, 1407, 1560, 1820, 1836, 1960
NciI CCSGG 1 cut(s) 2411
NcoI CCATGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 1813
NlaIV GGNNCC 4 cut(s) 706, 868, 1584, 2827
NmuCI GTSAC 2 cut(s) 1517, 2506
NsiI ATGCAT 4 cut(s) 567, 1285, 2002, 2621
NspI RCATGY 3 cut(s) 779, 1631, 2000
PaeR7I CTCGAG 1 cut(s) 1718
PagI TCATGA 2 cut(s) 747, 2397
PceI AGGCCT 1 cut(s) 2336
PciSI GCTCTTC 1 cut(s) 961
PctI GAATGC 3 cut(s) 858, 1384, 2619
PdmI GAANNNNTTC 3 cut(s) 1315, 1318, 2156
PflMI CCANNNNNTGG 2 cut(s) 1814, 2806
PfoI TCCNGGA 2 cut(s) 1834, 2409
PleI GAGTC 4 cut(s) 1353, 2564, 2742, 2928
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 4 cut(s) 1353, 2564, 2742, 2928
Ppu21I YACGTR 1 cut(s) 839
PpuMI RGGWCCY 1 cut(s) 896
PshAI GACNNNNGTC 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 896
Psp6I CCWGG 6 cut(s) 1389, 1405, 1558, 1818, 1834, 1958
PspCI CACGTG 1 cut(s) 839
PspEI GGTNACC 1 cut(s) 1458
PspFI CCCAGC 2 cut(s) 496, 1433
PspGI CCWGG 6 cut(s) 1389, 1405, 1558, 1818, 1834, 1958
PspN4I GGNNCC 4 cut(s) 706, 868, 1584, 2827
PspPI GGNCC 5 cut(s) 704, 896, 1123, 1556, 1582
PspPPI RGGWCCY 1 cut(s) 896
PspXI VCTCGAGB 1 cut(s) 1718
PstI CTGCAG 2 cut(s) 1087, 1185
PstNI CAGNNNCTG 4 cut(s) 1180, 1192, 1959, 1973
PsuI RGATCY 3 cut(s) 1079, 1874, 2866
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 6 cut(s) 326, 868, 1751, 2086, 2242, 2975
RsaNI GTAC 6 cut(s) 325, 867, 1750, 2085, 2241, 2974
RseI CAYNNNNRTG 4 cut(s) 583, 2189, 2526, 2565
SapI GCTCTTC 1 cut(s) 961
SaqAI TTAA 6 cut(s) 1061, 1479, 1601, 1766, 1917, 2946
Sau96I GGNCC 5 cut(s) 704, 896, 1123, 1556, 1582
SchI GAGTC 4 cut(s) 1354, 2564, 2743, 2929
ScrFI CCNGG 7 cut(s) 1391, 1407, 1560, 1820, 1836, 1960, 2411
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 7 cut(s) 67, 1198, 1292, 1384, 1960, 2009, 2808
SfcI CTRYAG 4 cut(s) 668, 1083, 1181, 2136
Sfr274I CTCGAG 1 cut(s) 1718
SinI GGWCC 3 cut(s) 896, 1123, 1582
SlaI CTCGAG 1 cut(s) 1718
SmiMI CAYNNNNRTG 4 cut(s) 583, 2189, 2526, 2565
SmlI CTYRAG 6 cut(s) 95, 1060, 1718, 2175, 2460, 2924
SmoI CTYRAG 6 cut(s) 95, 1060, 1718, 2175, 2460, 2924
SpeI ACTAGT 1 cut(s) 5
SseBI AGGCCT 1 cut(s) 2336
SsiI CCGC 4 cut(s) 179, 365, 1912, 1940
SspI AATATT 1 cut(s) 2157
SspMI CTAG 5 cut(s) 6, 38, 1247, 1308, 1638
StuI AGGCCT 1 cut(s) 2336
StyD4I CCNGG 7 cut(s) 1389, 1405, 1558, 1818, 1834, 1958, 2409
StyI CCWWGG 2 cut(s) 862, 2337
TaaI ACNGT 6 cut(s) 187, 1166, 1277, 1749, 2137, 2514
TaiI ACGT 4 cut(s) 578, 841, 2664, 2886
TaqI TCGA 5 cut(s) 52, 1413, 1719, 2124, 2385
TatI WGTACW 1 cut(s) 2973
Tru1I TTAA 6 cut(s) 1061, 1479, 1601, 1766, 1917, 2946
Tru9I TTAA 6 cut(s) 1061, 1479, 1601, 1766, 1917, 2946
TscAI CASTG 6 cut(s) 178, 1142, 1851, 2140, 2517, 2836
TseFI GTSAC 2 cut(s) 1517, 2506
Tsp45I GTSAC 2 cut(s) 1517, 2506
TspGWI ACGGA 2 cut(s) 1536, 2704
TspRI CASTG 6 cut(s) 178, 1142, 1851, 2140, 2517, 2836
Van91I CCANNNNNTGG 2 cut(s) 1814, 2806
Vha464I CTTAAG 1 cut(s) 1060
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 896, 1123, 1582
XapI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1048, 2078, 2225, 2469
XceI RCATGY 3 cut(s) 779, 1631, 2000
XhoI CTCGAG 1 cut(s) 1718
XmnI GAANNNNTTC 3 cut(s) 1315, 1318, 2156
XspI CTAG 5 cut(s) 6, 38, 1247, 1308, 1638
ZraI GACGTC 1 cut(s) 576
Zsp2I ATGCAT 4 cut(s) 567, 1285, 2002, 2621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.