MD01G1104900.v1.1

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007939
Physical Location & Seq
Forward (+)
21801712 .. 21808713
7002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1104900.v1.1.491

Sequence Viewer

Length: 3042 bp
ATGCTATCAACCCTAGATAATAATCTGCAAGGATCCAATGGGAATTTAGAAGATGCTTTACAGAGTGAGCTTGAATTGATTCTGAGAGAGCAGCGCAATCAGCATGCCATTCATCGGGAGAGGGACATGAATATGTATAGGAGCGGCAGTGCCCCGCCAACTGTTGAGGGTTCGCTAACTGCTGTGGGTAGTCTCTTTAGGAACTCAGATTTTAGGGATGTGACTAGTAGGATTAGTAATAACAATGGGGTTTTGTCCGAAGATGAGATTCGATCTCATCCTGCTTATCTTTCGTACTACTATTCGCATGATAATATTAACCCAAGATTGCCTCCACCGTCGTTGTCGAGAGAGGATTGGCGTGTTGCACAAAGGTTTCAGGGGGGCGGGTCTTCTTTTGAAGGCATTGGGGACTGGAGGAGGAAGAAGTTGGCTGATGATGGTGATAGTTTGTCGCTGTTTTCCACACAGTTGGGACTACAAAAGGCAGAGGATGAGTTGATGGGATTGAGGAATGCTAAAGGTTGCAATCTCCCTAAACAGACATCATCTGAGTGGCTTGATAGAAGCTCAGATGGTTTGATTGGTTTGTCGACAACTGGGTTTGGTGGAAGGAGAAAGAGTTTTGCTGACATTTTACAGGAAGGACTTGATCAAACTGCGTCCTTGTCAGGTCCCCTGTCAAGGCCATCAAGTCATAACACTTTTGGTGATATCATGGATCCCACAGGCATGACTGATCCCCATCCAGTTGGGTTATGTAATGGAGTGGAGTCTGCAGAGGGTATGCATAATGGAGCAGCTTCACATGGCCTGGTGGGAGTTCGGAGCCATGGCCCAATGGCTTCTCATTCCTTTGCATCTGCTGTGGGTCCTTCTTTGCCAAGAAGCAGAACCCCTGAACAACTATTTGGCAGGTCCACCAGTTCTGGTCTCCTCCCTGTAGGGAGTAGAGTATTCCCTACCGAGAGGAAGAATGTTGCTAGTCCAGATATTAAAAATGATCGTTCTTCCAGCATGAATGATCTGGCGGATATTTCAGCCAATTTTTCTAGCTTAAGCCTATCAAAAATTAGACCTGTAGATGATGATAGATATATTCAGTCGCAGCTTCATGTGGATCTTGAAAATCAGCATGATTTTCTGTTTAAGATGCCAAATGGGCATCAGAGGCTGCAGCAGGAACTGTTAGAGAAGGCAAATGCTGAAATTTTTTCTCTTCCTACTAATCATGCTCATCTAGCAAAGAAAAATGGAATCATAACAAACCGTACTACATCCTCTGGGCAAGCTAATTTTTCAAGAAGAACTTCTTCTCCCAGTCTGTACTCAAAAGGGAGTTCTTCAGGCTTTGGAGCTTTGGAAGGATCTAGTGTTCATTACCAAGAAGCAAATCCACCAGGTATGGACTTTGATGGGCATGTTGCCGGGGCCTATCCTGTAAATCTGAAGTTGAATATGTCAAGTGATAATCATCTTGGTGCAGTAGCTATTGGTGATGAACAAAGTCTGAACAGACTGGGAAACAAAGTAGGGCCTGGTCTTCATTCTTCTGTTATGGACCCATCTTATATTCAGTTTTTGCAAAGAACTGATTATGCAGCACGTAATGTAACTAATCCAAGTGGTTATCCTCTGAGCAAAAACCACTTTGGCACTTTGCATGGAGACTTAGAGGGGCTTCAGAAGGCATATCTTGAGGCATTGCTTGCTCAAAGGAAGCAACAGTATGATTTGTCAATTTTAGGTAAATCTGGCGGTTTTAATCATGGTTATTATGGGAACCCACCATATGGGCTTGGCATGTATCCAGGAAATCCGATGGCAAATTCGCTTCATCCTTCTATTGGGTATGGTAGTCCAATGTTTGAGAGTGAGAAGATTGCCCGCTTTAATTCTATGTTGAGAAGTTCAATGGGAGGGTCGGTTAGCTCCTGGAACTCAGATATTGGAAACAACCTAGAAGGACGACATGTATCATCATTATTAGATGAATTCAAGAACAATAAGAACAAGTCTTTTGAATTTGCGGACATTGTTGATCACGTAATTGAATTCAGTACCGACCAGTATGGAAGTCGCTTTATTCAGCAGAAACTAGAAACAGCCACTGTGGAAGAAAAAATGAAGATATTTCCAGAGATTATTCCTCATGCTCGCGCCTTGATGACTGATGTATTCGGAAATTATGTCGTACAGAAATTTTTTGAGCATGGTACAGAAAGCCAAAGAAAAGAGTTAACCAGCCAACTTACTGGCCATGTTTTGCCTCTCAGTCTTCAAATGTATGGTTGTAGAGTGATCCAGAAGGCTTTGGAAGTGGTTGATGTGAATCATCAGGCACAGATGGTGTCGGAGCTTGATGGTTCAATCATGAAATGCGTCCGTGATCAGAATGGGAATCATGTCATTCAGAAGTGTATAGAATGTATCCCTCAAGATAAAATTCAATTTATAATTTCATCCTTCTATGGGCAAGTTGTCACACTATCCACTCATCCTTATGGATGCCGTGTTATACAGAGGGTTCTGGAACATTGCAATGATTCAAACACACAACAAATAATCATGGATGAAATCATGCAATCTGTATGCATTTTGGCACAAGATCAATATGGAAATTACGTCATTCAGCACGTCCTTGAACATGGTAAACCGCATGAACGGACTGCAATTATCCACAAGCTCGCAGGACAAATTGTGAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAGAAGTGCTTAACTTTTGGGAATGCTGATGAGCGCCAGTTTTTAGTGAATGAGATGCTGGGTTCAACTGATGAAAATGAGCCCTTACAGGCCATGATGAAAGATCCCTTTGGAAACTACGTTGTGCAGAAGGTTCTTGAGACTTGTGATGATCAGAGTCTTGAGTTGATTCTTTCTCGCATTAAGGTTCATTTGACTGCCCTGAAGAAGTATACCTACGGCAAACATATCGTTTCTCGTGTCGAGAAACTCATCACAACTGGAGAAAGGCGCATAGGGTTGTCAGCCTCGTGTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1014

Amino Acids

111.9

Weight (kDa)

6.31

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 306 - 676 3.3e-109 Nucleic acid binding protein NABP
PUF PF00806 682 - 716 6.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 721 - 749 6e-08 Pumilio-family RNA binding repeat
PUF PF00806 754 - 787 5.5e-09 Pumilio-family RNA binding repeat
PUF PF00806 826 - 861 7.9e-14 Pumilio-family RNA binding repeat
PUF PF00806 864 - 896 2.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 899 - 933 7.9e-08 Pumilio-family RNA binding repeat
PUF PF00806 945 - 974 6.1e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2456
Acc36I ACCTGC 1 cut(s) 906
AccB7I CCANNNNNTGG 1 cut(s) 1793
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 2 cut(s) 593, 2951
AccII CGCG 1 cut(s) 2160
AciI CCGC 8 cut(s) 144, 155, 387, 1031, 1758, 1888, 2030, 2657
AclWI GGATC 9 cut(s) 27, 40, 716, 729, 734, 1128, 1375, 2296, 2837
AcoI YGGCCR 1 cut(s) 2257
AcsI RAATTY 8 cut(s) 43, 1209, 1828, 1994, 2024, 2054, 2201, 2445
AcuI CTGAAG 4 cut(s) 1329, 1469, 1667, 2963
AfaI GTAC 6 cut(s) 296, 1273, 1328, 2062, 2196, 2218
AfiI CCNNNNNNNGG 5 cut(s) 114, 684, 1793, 1847, 2472
AflII CTTAAG 1 cut(s) 1057
AflIII ACRYGT 1 cut(s) 1972
AhlI ACTAGT 1 cut(s) 224
AjiI CACGTC 1 cut(s) 2638
AjnI CCWGG 5 cut(s) 813, 1399, 1537, 1810, 1934
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AloI GAACNNNNNNTCC 4 cut(s) 1300, 1332, 1359, 1391
Alw26I GTCTC 4 cut(s) 197, 938, 1662, 2873
AlwI GGATC 9 cut(s) 27, 40, 716, 729, 734, 1128, 1375, 2296, 2837
AlwNI CAGNNNCTG 3 cut(s) 1174, 1186, 2111
AoxI GGCC 7 cut(s) 686, 811, 835, 1431, 1535, 2257, 2829
ApeKI GCWGC 6 cut(s) 91, 800, 1108, 1174, 1177, 1601
ApoI RAATTY 8 cut(s) 43, 1209, 1828, 1994, 2024, 2054, 2201, 2445
Asp700I GAANNNNTTC 3 cut(s) 78, 1309, 1312
AspLEI GCGC 4 cut(s) 96, 2162, 2775, 3012
AspS9I GGNCC 7 cut(s) 674, 836, 872, 918, 1431, 1535, 1561
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 3 cut(s) 455, 722, 1508
AvaII GGWCC 4 cut(s) 674, 872, 918, 1561
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 2259
BamHI GGATCC 2 cut(s) 32, 721
BanII GRGCYC 1 cut(s) 2823
BarI GAAGNNNNNNTAC 4 cut(s) 42, 74, 2939, 2971
BauI CACGAG 2 cut(s) 2976, 3028
BbsI GAAGAC 3 cut(s) 384, 1535, 2270
BbvI GCAGC 6 cut(s) 103, 812, 1120, 1161, 1189, 1613
BceAI ACGGC 2 cut(s) 2496, 2974
BcgI CGANNNNNNTGC 2 cut(s) 2950, 2984
BciT130I CCWGG 5 cut(s) 815, 1401, 1539, 1812, 1936
BciVI GTATCC 2 cut(s) 1818, 2441
BclI TGATCA 4 cut(s) 652, 2041, 2389, 2890
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 4 cut(s) 197, 938, 1662, 2873
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 8 cut(s) 14, 225, 984, 1053, 1241, 1371, 1961, 2099
BfmI CTRYAG 4 cut(s) 777, 942, 1080, 1175
BfoI RGCGCY 1 cut(s) 2776
BfrI CTTAAG 1 cut(s) 1057
BfuAI ACCTGC 1 cut(s) 906
BfuI GTATCC 2 cut(s) 1818, 2441
BglI GCCNNNNNGGC 1 cut(s) 1162
BisI GCNGC 7 cut(s) 92, 145, 801, 1109, 1175, 1178, 1602
BlsI GCNGC 7 cut(s) 93, 146, 802, 1110, 1176, 1179, 1603
Bme1390I CCNGG 6 cut(s) 815, 1401, 1429, 1539, 1812, 1936
Bme18I GGWCC 4 cut(s) 674, 872, 918, 1561
BmgBI CACGTC 1 cut(s) 2638
BmgT120I GGNCC 7 cut(s) 674, 836, 872, 918, 1431, 1535, 1561
BmiI GGNNCC 8 cut(s) 34, 676, 723, 830, 873, 1432, 1563, 1784
BmrFI CCNGG 6 cut(s) 815, 1401, 1429, 1539, 1812, 1936
BmrI ACTGGG 3 cut(s) 609, 1314, 1529
BmsI GCATC 6 cut(s) 43, 869, 1143, 1174, 2498, 2784
BmuI ACTGGG 3 cut(s) 609, 1314, 1529
BpiI GAAGAC 3 cut(s) 384, 1535, 2270
BpmI CTGGAG 2 cut(s) 436, 3021
BpuEI CTTGAG 4 cut(s) 1718, 2421, 2897, 2921
BpuMI CCSGG 1 cut(s) 1429
BsaAI YACGTR 2 cut(s) 1607, 2047
BsaBI GATNNNNATC 4 cut(s) 21, 744, 1473, 2331
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 2 cut(s) 832, 1428
BsaXI ACNNNNNCTCC 8 cut(s) 133, 163, 607, 637, 1300, 1330, 1911, 1941
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 684, 1793, 1847, 2472
Bse3DI GCAATG 3 cut(s) 1703, 2536, 2548
Bse8I GATNNNNATC 4 cut(s) 21, 744, 1473, 2331
BseBI CCWGG 5 cut(s) 815, 1401, 1539, 1812, 1936
BseDI CCNNGG 2 cut(s) 832, 1428
BseJI GATNNNNATC 4 cut(s) 21, 744, 1473, 2331
BseLI CCNNNNNNNGG 5 cut(s) 114, 684, 1793, 1847, 2472
BseMI GCAATG 3 cut(s) 1703, 2536, 2548
BseMII CTCAG 7 cut(s) 74, 219, 543, 585, 1628, 1956, 2287
BseRI GAGGAG 2 cut(s) 433, 926
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 6 cut(s) 103, 812, 1120, 1161, 1189, 1613
BseYI CCCAGC 1 cut(s) 2797
BsgI GTGCAG 2 cut(s) 1503, 2885
Bsh1236I CGCG 1 cut(s) 2160
BshFI GGCC 7 cut(s) 688, 813, 837, 1433, 1537, 2259, 2831
BsiSI CCGG 1 cut(s) 1428
BslFI GGGAC 4 cut(s) 137, 425, 489, 660
BslI CCNNNNNNNGG 5 cut(s) 114, 684, 1793, 1847, 2472
BsmAI GTCTC 4 cut(s) 197, 938, 1662, 2873
BsmFI GGGAC 4 cut(s) 137, 425, 489, 660
BsmI GAATGC 2 cut(s) 520, 2767
BsnI GGCC 7 cut(s) 688, 813, 837, 1433, 1537, 2259, 2831
Bso31I GGTCTC 1 cut(s) 938
Bsp1286I GDGCHC 2 cut(s) 154, 2823
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 8 cut(s) 144, 155, 387, 1031, 1758, 1888, 2030, 2657
BspANI GGCC 7 cut(s) 688, 813, 837, 1433, 1537, 2259, 2831
BspCNI CTCAG 7 cut(s) 75, 218, 544, 584, 1629, 1955, 2286
BspFNI CGCG 1 cut(s) 2160
BspHI TCATGA 1 cut(s) 2373
BspLI GGNNCC 8 cut(s) 34, 676, 723, 830, 873, 1432, 1563, 1784
BspMAI CTGCAG 2 cut(s) 781, 1179
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 9 cut(s) 27, 40, 716, 729, 734, 1128, 1375, 2296, 2837
BspTI CTTAAG 1 cut(s) 1057
BspTNI GGTCTC 1 cut(s) 938
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 3 cut(s) 1703, 2536, 2548
BssECI CCNNGG 2 cut(s) 832, 1428
BssNAI GTATAC 1 cut(s) 2952
BssSI CACGAG 2 cut(s) 2976, 3028
BssT1I CCWWGG 1 cut(s) 832
Bst1107I GTATAC 1 cut(s) 2952
Bst2BI CACGAG 2 cut(s) 2976, 3028
Bst2UI CCWGG 5 cut(s) 815, 1401, 1539, 1812, 1936
Bst4CI ACNGT 7 cut(s) 163, 339, 471, 1188, 1271, 1728, 2113
Bst6I CTCTTC 1 cut(s) 1224
BstAFI CTTAAG 1 cut(s) 1057
BstAPI GCANNNNNTGC 1 cut(s) 1709
BstBAI YACGTR 2 cut(s) 1607, 2047
BstC8I GCNNGC 6 cut(s) 105, 1290, 1710, 1888, 2158, 2688
BstDEI CTNAG 8 cut(s) 83, 205, 552, 571, 1637, 1672, 1942, 2273
BstDSI CCRYGG 1 cut(s) 832
BstFNI CGCG 1 cut(s) 2160
BstH2I RGCGCY 1 cut(s) 2776
BstHHI GCGC 4 cut(s) 96, 2162, 2775, 3012
BstMAI GTCTC 4 cut(s) 197, 938, 1662, 2873
BstMWI GCNNNNNNNGC 5 cut(s) 100, 1162, 1171, 1241, 1709
BstNI CCWGG 5 cut(s) 815, 1401, 1539, 1812, 1936
BstNSI RCATGY 4 cut(s) 107, 1424, 1807, 1976
BstSCI CCNGG 6 cut(s) 813, 1399, 1427, 1537, 1810, 1934
BstSFI CTRYAG 4 cut(s) 777, 942, 1080, 1175
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 2160
BstV1I GCAGC 6 cut(s) 103, 812, 1120, 1161, 1189, 1613
BstV2I GAAGAC 3 cut(s) 384, 1535, 2270
BstX2I RGATCY 5 cut(s) 32, 721, 1120, 1367, 2842
BstXI CCANNNNNNTGG 3 cut(s) 472, 752, 2255
BstYI RGATCY 5 cut(s) 32, 721, 1120, 1367, 2842
BstZ17I GTATAC 1 cut(s) 2952
BsuI GTATCC 2 cut(s) 1818, 2441
BsuRI GGCC 7 cut(s) 688, 813, 837, 1433, 1537, 2259, 2831
BtgI CCRYGG 1 cut(s) 832
BtrI CACGTC 1 cut(s) 2638
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 154, 2109
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 6 cut(s) 105, 1290, 1710, 1888, 2158, 2688
CaiI CAGNNNCTG 3 cut(s) 1174, 1186, 2111
CciI TCATGA 1 cut(s) 2373
CfoI GCGC 4 cut(s) 96, 2162, 2775, 3012
Cfr13I GGNCC 7 cut(s) 674, 836, 872, 918, 1431, 1535, 1561
CseI GACGC 2 cut(s) 651, 2371
CsiI ACCWGGT 1 cut(s) 1399
Csp6I GTAC 6 cut(s) 295, 1272, 1327, 2061, 2195, 2217
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviQI GTAC 6 cut(s) 295, 1272, 1327, 2061, 2195, 2217
DdeI CTNAG 8 cut(s) 83, 205, 552, 571, 1637, 1672, 1942, 2273
EaeI YGGCCR 1 cut(s) 2257
Eam1104I CTCTTC 1 cut(s) 1224
EarI CTCTTC 1 cut(s) 1224
EciI GGCGGA 1 cut(s) 1046
Eco130I CCWWGG 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 2823
Eco31I GGTCTC 1 cut(s) 938
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 4 cut(s) 674, 872, 918, 1561
Eco57I CTGAAG 4 cut(s) 1329, 1469, 1667, 2963
EcoO109I RGGNCCY 4 cut(s) 674, 872, 1431, 1535
EcoRI GAATTC 2 cut(s) 1994, 2054
EcoRII CCWGG 5 cut(s) 813, 1399, 1537, 1810, 1934
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 832
EcoT22I ATGCAT 2 cut(s) 792, 2597
EcoT38I GRGCYC 1 cut(s) 2823
ErhI CCWWGG 1 cut(s) 832
FalI AAGNNNNNCTT 4 cut(s) 1294, 1326, 1297, 1329
FaqI GGGAC 4 cut(s) 137, 425, 489, 660
FauI CCCGC 3 cut(s) 162, 380, 1895
FauNDI CATATG 1 cut(s) 1792
FbaI TGATCA 4 cut(s) 652, 2041, 2389, 2890
FblI GTMKAC 2 cut(s) 593, 2951
Fnu4HI GCNGC 7 cut(s) 92, 145, 801, 1109, 1175, 1178, 1602
FriOI GRGCYC 1 cut(s) 2823
Fsp4HI GCNGC 7 cut(s) 92, 145, 801, 1109, 1175, 1178, 1602
FspBI CTAG 8 cut(s) 14, 225, 984, 1053, 1241, 1371, 1961, 2099
GlaI GCGC 4 cut(s) 95, 2161, 2774, 3011
GluI GCNGC 7 cut(s) 92, 145, 801, 1109, 1175, 1178, 1602
GsaI CCCAGC 1 cut(s) 2801
GsuI CTGGAG 2 cut(s) 436, 3021
HaeII RGCGCY 1 cut(s) 2776
HaeIII GGCC 7 cut(s) 688, 813, 837, 1433, 1537, 2259, 2831
HapII CCGG 1 cut(s) 1428
HgaI GACGC 2 cut(s) 651, 2371
HhaI GCGC 4 cut(s) 96, 2162, 2775, 3012
Hin6I GCGC 4 cut(s) 94, 2160, 2773, 3010
HinP1I GCGC 4 cut(s) 94, 2160, 2773, 3010
HincII GTYRAC 2 cut(s) 594, 2241
HindII GTYRAC 2 cut(s) 594, 2241
HpaI GTTAAC 1 cut(s) 2241
HpaII CCGG 1 cut(s) 1428
HphI GGTGA 3 cut(s) 455, 722, 1508
Hpy166II GTNNAC 5 cut(s) 594, 921, 2241, 2654, 2952
Hpy8I GTNNAC 5 cut(s) 594, 921, 2241, 2654, 2952
Hpy99I CGWCG 1 cut(s) 343
HpyCH4III ACNGT 7 cut(s) 163, 339, 471, 1188, 1271, 1728, 2113
HpyCH4IV ACGT 5 cut(s) 1606, 2046, 2625, 2637, 2859
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 1162, 1171, 1241, 1709
HpyF3I CTNAG 8 cut(s) 83, 205, 552, 571, 1637, 1672, 1942, 2273
HpySE526I ACGT 5 cut(s) 1606, 2046, 2625, 2637, 2859
HspAI GCGC 4 cut(s) 94, 2160, 2773, 3010
Ksp22I TGATCA 4 cut(s) 652, 2041, 2389, 2890
KspAI GTTAAC 1 cut(s) 2241
LmnI GCTCC 6 cut(s) 141, 797, 828, 1355, 1937, 2356
Lsp1109I GCAGC 6 cut(s) 103, 812, 1120, 1161, 1189, 1613
LweI GCATC 6 cut(s) 43, 869, 1143, 1174, 2498, 2784
MabI ACCWGGT 1 cut(s) 1399
MaeI CTAG 8 cut(s) 14, 225, 984, 1053, 1241, 1371, 1961, 2099
MaeII ACGT 5 cut(s) 1606, 2046, 2625, 2637, 2859
MaeIII GTNAC 3 cut(s) 220, 1612, 2482
MbiI CCGCTC 1 cut(s) 144
MflI RGATCY 5 cut(s) 32, 721, 1120, 1367, 2842
MhlI GDGCHC 2 cut(s) 154, 2823
MlsI TGGCCA 1 cut(s) 2259
MluNI TGGCCA 1 cut(s) 2259
MlyI GAGTC 3 cut(s) 782, 2719, 2905
MmeI TCCRAC 1 cut(s) 2334
Mox20I TGGCCA 1 cut(s) 2259
Mph1103I ATGCAT 2 cut(s) 792, 2597
MroXI GAANNNNTTC 3 cut(s) 78, 1309, 1312
MscI TGGCCA 1 cut(s) 2259
MseI TTAA 9 cut(s) 318, 996, 1058, 1149, 1764, 1893, 2240, 2750, 2922
MslI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1479, 2502, 2541
Msp20I TGGCCA 1 cut(s) 2259
MspCI CTTAAG 1 cut(s) 1057
MspI CCGG 1 cut(s) 1428
MspR9I CCNGG 6 cut(s) 815, 1401, 1429, 1539, 1812, 1936
Mva1269I GAATGC 2 cut(s) 520, 2767
MvaI CCWGG 5 cut(s) 815, 1401, 1539, 1812, 1936
MvnI CGCG 1 cut(s) 2160
MwoI GCNNNNNNNGC 5 cut(s) 100, 1162, 1171, 1241, 1709
NciI CCSGG 1 cut(s) 1429
NcoI CCATGG 1 cut(s) 832
NdeI CATATG 1 cut(s) 1792
NlaIV GGNNCC 8 cut(s) 34, 676, 723, 830, 873, 1432, 1563, 1784
NmuCI GTSAC 2 cut(s) 220, 2482
NsiI ATGCAT 2 cut(s) 792, 2597
NspI RCATGY 4 cut(s) 107, 1424, 1807, 1976
PaeI GCATGC 1 cut(s) 107
PagI TCATGA 1 cut(s) 2373
PciI ACATGT 1 cut(s) 1972
PctI GAATGC 2 cut(s) 520, 2767
PdmI GAANNNNTTC 3 cut(s) 78, 1309, 1312
PfeI GAWTC 7 cut(s) 79, 268, 1257, 2332, 2401, 2546, 2908
PflMI CCANNNNNTGG 1 cut(s) 1793
PfoI TCCNGGA 2 cut(s) 1810, 1934
PkrI GCNGC 7 cut(s) 93, 146, 802, 1110, 1176, 1179, 1603
PleI GAGTC 3 cut(s) 781, 2718, 2904
PpsI GAGTC 3 cut(s) 781, 2718, 2904
Ppu21I YACGTR 2 cut(s) 1607, 2047
PpuMI RGGWCCY 2 cut(s) 674, 872
PscI ACATGT 1 cut(s) 1972
PsiI TTATAA 1 cut(s) 2456
Psp5II RGGWCCY 2 cut(s) 674, 872
Psp6I CCWGG 5 cut(s) 813, 1399, 1537, 1810, 1934
PspFI CCCAGC 1 cut(s) 2797
PspGI CCWGG 5 cut(s) 813, 1399, 1537, 1810, 1934
PspN4I GGNNCC 8 cut(s) 34, 676, 723, 830, 873, 1432, 1563, 1784
PspPI GGNCC 7 cut(s) 674, 836, 872, 918, 1431, 1535, 1561
PspPPI RGGWCCY 2 cut(s) 674, 872
PstI CTGCAG 2 cut(s) 781, 1179
PstNI CAGNNNCTG 3 cut(s) 1174, 1186, 2111
PsuI RGATCY 5 cut(s) 32, 721, 1120, 1367, 2842
RsaI GTAC 6 cut(s) 296, 1273, 1328, 2062, 2196, 2218
RsaNI GTAC 6 cut(s) 295, 1272, 1327, 2061, 2195, 2217
RseI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1479, 2502, 2541
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 9 cut(s) 318, 996, 1058, 1149, 1764, 1893, 2240, 2750, 2922
SatI GCNGC 7 cut(s) 92, 145, 801, 1109, 1175, 1178, 1602
Sau96I GGNCC 7 cut(s) 674, 836, 872, 918, 1431, 1535, 1561
SchI GAGTC 3 cut(s) 782, 2719, 2905
ScrFI CCNGG 6 cut(s) 815, 1401, 1429, 1539, 1812, 1936
SduI GDGCHC 2 cut(s) 154, 2823
SexAI ACCWGGT 1 cut(s) 1399
SfaNI GCATC 6 cut(s) 43, 869, 1143, 1174, 2498, 2784
SfcI CTRYAG 4 cut(s) 777, 942, 1080, 1175
SinI GGWCC 4 cut(s) 674, 872, 918, 1561
SmiMI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1479, 2502, 2541
SmlI CTYRAG 5 cut(s) 1057, 1697, 2436, 2876, 2900
SmoI CTYRAG 5 cut(s) 1057, 1697, 2436, 2876, 2900
SpeI ACTAGT 1 cut(s) 224
SphI GCATGC 1 cut(s) 107
SsiI CCGC 8 cut(s) 144, 155, 387, 1031, 1758, 1888, 2030, 2657
SspI AATATT 1 cut(s) 316
SspMI CTAG 8 cut(s) 14, 225, 984, 1053, 1241, 1371, 1961, 2099
StyD4I CCNGG 6 cut(s) 813, 1399, 1427, 1537, 1810, 1934
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 7 cut(s) 163, 339, 471, 1188, 1271, 1728, 2113
TaiI ACGT 5 cut(s) 1609, 2049, 2628, 2640, 2862
TaqI TCGA 4 cut(s) 271, 347, 593, 2982
TatI WGTACW 1 cut(s) 1326
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 7 cut(s) 79, 268, 1257, 2332, 2401, 2546, 2908
Tru1I TTAA 9 cut(s) 318, 996, 1058, 1149, 1764, 1893, 2240, 2750, 2922
Tru9I TTAA 9 cut(s) 318, 996, 1058, 1149, 1764, 1893, 2240, 2750, 2922
TscAI CASTG 2 cut(s) 154, 2116
TseFI GTSAC 2 cut(s) 220, 2482
TseI GCWGC 6 cut(s) 91, 800, 1108, 1174, 1177, 1601
Tsp45I GTSAC 2 cut(s) 220, 2482
TspGWI ACGGA 2 cut(s) 2375, 2680
TspRI CASTG 2 cut(s) 154, 2116
Van91I CCANNNNNTGG 1 cut(s) 1793
Vha464I CTTAAG 1 cut(s) 1057
VpaK11BI GGWCC 4 cut(s) 674, 872, 918, 1561
XapI RAATTY 8 cut(s) 43, 1209, 1828, 1994, 2024, 2054, 2201, 2445
XceI RCATGY 4 cut(s) 107, 1424, 1807, 1976
XmiI GTMKAC 2 cut(s) 593, 2951
XmnI GAANNNNTTC 3 cut(s) 78, 1309, 1312
XspI CTAG 8 cut(s) 14, 225, 984, 1053, 1241, 1371, 1961, 2099
Zsp2I ATGCAT 2 cut(s) 792, 2597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.