Rh1DG304600

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007939
Physical Location & Seq
Forward (+)
53204717 .. 53209855
5139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG304600.1

Sequence Viewer

Length: 3054 bp
ATGGCTAGTGGGGGAAAGATAGATATGCTATCAACGCTAGATAATAATGTGCGAGGTTCAAATGGGAATTTAGAAGATGCTTTGCAGAGTGAGCTTGAGCTGATTCTAAGGGAGCAGCGCAATCAGCACGCCATTCATCGTGATAGGGAAATGAATATGTATAGGAGTGGCAGTGCACCGCCAACTGTGGAGGGATCGCTGAGTGCTGTGGGTAGTCTGTTTAGGAATTCTGATTTTAGGGATAGGGACATGAGAAGTAGGGTTAGTAGTAACAATGGGGGTTTGTCTGAAGATGAGATTCGCTCACATCCTGCTTACCTTTCGTACTATTATTCACATGACAATATCAACCCAAGATTGCCTCCGCCATTGTTGTCAAGAGAGGATTGGCGTGTTGCGCAGAGGTTTCAGAGTGGGTCTTCTTTTGAAGGTGCTGGAGATTGGAGGAAGAACAATTTAGTTGGTGATGGTGATAAGTCGTTGTTTTCAATGCAGCTGGGGCTTTCTGCACAGAATGCAGATAATGACTTGATCAAATTTAGAAATGCCAATGGGAGTAGTATGCATAGACAGACGTCATCTGAGTGGCTTGACAGAAATTCGAATAGTTTGACTGGATTCCCTACAAATGGACTTGGTGCAAGGAGGAAGAGTTTTGCTGACATTCTACAGGAAGGAAATGATCATCCAGCTTCCTTGTCAGGCCCCCTGTCAAGGCCATCAAGCCGTAATGCTTTTGGTGATGTCATGAATTCAACCGGCATGGCTGATCCACATCCAGTAGGGTTGTGTAATGGAGTGGATTCCATAGAGGGGTTGCACACCGGAGCAGCTCCACGTGGCTTGATAGGAATGCAGAGCCATGGTACCCCAGTTTCTCATTCATTTGCATCTGCTGGAGGTCCTTCTTTGTCAAGAAGCAGAACCCCTGAACCACAATTATTTGGAAGTTCTGGTCTTCCTCCTGTGGGAAGAGCGCTTCCTAATGTTGCTGGTGCAGATATCCAAAGTGATAGTTCGCCTGCCATGACTGAGTTGCCCAATATGACAACAAATTTATCTGGCTTAAGCCTCTCAAAAGTTAGATCTGCAGATGAAGATAGTCGTCTTCAGTCTCAGCTTCGTATGGACCTTCATAATCAGTGTGATTTTCTGTTCAACACACCAAACAGTCAGATTCAGAGGCTGCAGCAGCAACTGATAGAGAAGTCAAATGCTGAAAACTTTTCCCTTTCTACAAATTATGCTCATTTGGCAAATAAAAATGGGATTCTAACAAACCGTAATGCATCAAATGGGCATGTTCATTTTCCTAGAAGAACTTCTTCTCCCAGTCTTTACTCAAACGGGAGTTCTTCAGGACTTGGAACTTTGGAAGGGTTGAGTGCTCATTACCAGGATGCAAATACCCCAGGAATCGACTTTGATGGTCATGCTGCCGGGGCATATCCTATAAATCAGAAGTTGAACAATCATCTCGATGCAGGTTTTACTGGTAGTGGTGACGGACAAAGTATGAACAGATTAGGGAACAAAGTAGGGCCTGGCCTTCATTCTTCGTCTGTGGACCCATCATATATTCAGTTAATGCAAAGAACTGATTATGCAGCACATGCTCTGGCTAGTCCAAGTGGTTATTCTCTGAGTAAAAATAACTTTGGCAATTTGCATGGAGACTTAGAGGGGCTTCAGAAAGCATACCTCGAGGCAATACTTGCTCAAAGGAAGCAACAGTACGAGTTGTCCCTTTTAAGTCAATCTGGGGGGTTTGATCATGGTTATTTTGGGAATCCATCATATGGCCTTGGGATGACATATCCAGGAATCTCAGTGGCAAACTCTGTTCTTCCTTCTGTTGGATCCGGAAGTTCAATGTTTCAGAATGAGAAGATTTCCCGCTTTAATTCTGTGATGAGAAGTTCAGTGGGAGGTTCAGTCAGCACCTGGCACTCAGATGCTGGAAATGACTTTGAAGGAAGACATGCATCATCATTATTAGATGAGTTCAAGAACAACAAGAATAAGTCTTTTGAACTTGCAGATATTGTTGATCACGTAGTTGAATTCAGTACCGATCAGTATGGAAGTCGCTTTATCCAGCAGAAACTCGAAACTGCCACTGTAGAAGAAAAAATGAAAATATTCCCTGAGATTATTCCTCATGCTCGCACCTTGATGACTGATGTTTTTGGAAATTATGTCATACAGAAATTCTTTGAGCATGGTACAGAAAGCCAAAGAAATGAATTGACGAACCAACTTACTGGCCATGTTTTGCCTCTCAGTCTGCAAATGTATGGTTGTAGAGTGATTCAGAAGGCCTTGGAAGTGGTTGACAAGGATCATCAGGCTCAAATGGTGTCAGAACTCGATGGTTCAGTCATGAAATGTGTCCGTGATCAAAATGGGAATCATGTTATTCAGAAGAGTATAGAATGTGTCCCTCAAGATCAAATTCAGTTCATCATTTCATCCTTCTATGGGCAAGTTGTCACACTGTCCACCCATCCTTATGGCTGCCGTGTCATACAGAGGGTTCTGGAACATTGTGATGACTCAAAGACGCAAGAAATTATCATGGATGAAGTCATGCAATCTGTATGCATTCTGGCACAGGATCAGTATGGGAATTATGTTATTCAGCACGTCCTTGAGCATGGTAAACCACATGAACGGTCTGCAATTATTCACAAGCTTGCTGGACAAATTGTAAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAAAAGTGCTTGACTTTTGGGAGTCCTGAGGAGCGCCAGTTGTTGGTGAAAGAGATGCTTGGTTCCACTGATGAAAATGAGCCATTGCAGGCCATGATGAAAGATCCATTTGGAAACTACGTCGTGCAGAAGGTTCTGGAGACCTGTGATGATCAGAGTCTTGAGTTGATTGTTTCTCGCATTAAGGTTCATTTGACTGCCTTGAAGAAGTACACCTATGGTAAACATATTGTTTCCCGTGTTGAGAAACTCATCACAACTGGAGAAAGGCGCATAGGATTGTCATCCTCAGTTTCTTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1017

Amino Acids

111.56

Weight (kDa)

6.2

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 317 - 680 1.4e-103 Nucleic acid binding protein NABP
PUF PF00806 685 - 719 1.7e-11 Pumilio-family RNA binding repeat
PUF PF00806 723 - 754 1.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 757 - 790 2.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 829 - 863 7.8e-13 Pumilio-family RNA binding repeat
PUF PF00806 867 - 899 2.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 902 - 935 1.8e-08 Pumilio-family RNA binding repeat
PUF PF00806 948 - 977 4.5e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 578
AbsI CCTCGAGG 1 cut(s) 1703
Acc16I TGCGCA 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 1475
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 2 cut(s) 1799, 2791
AccIII TCCGGA 1 cut(s) 1862
AciI CCGC 3 cut(s) 179, 365, 1897
AclWI GGATC 7 cut(s) 202, 764, 1854, 1867, 2349, 2625, 2846
AcoI YGGCCR 1 cut(s) 2266
AcsI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
AcuI CTGAAG 4 cut(s) 309, 1094, 1341, 1673
AcvI CACGTG 1 cut(s) 839
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 6 cut(s) 326, 868, 1736, 2071, 2227, 2960
AfeI AGCGCT 1 cut(s) 978
AfiI CCNNNNNNNGG 8 cut(s) 629, 714, 813, 968, 1799, 1856, 2481, 2791
AflII CTTAAG 1 cut(s) 1066
AjiI CACGTC 1 cut(s) 2647
AjnI CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
AjuI GAANNNNNNNTTGG 2 cut(s) 1621, 1653
AloI GAACNNNNNNTCC 2 cut(s) 1312, 1344
AluBI AGCT 7 cut(s) 94, 100, 496, 692, 833, 1120, 2695
AluI AGCT 7 cut(s) 94, 100, 496, 692, 833, 1120, 2695
Alw21I GWGCWC 2 cut(s) 178, 1390
Alw26I GTCTC 3 cut(s) 1119, 1668, 2882
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 7 cut(s) 202, 764, 1854, 1867, 2349, 2625, 2846
AlwNI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
Ama87I CYCGRG 1 cut(s) 1703
Aor13HI TCCGGA 1 cut(s) 1862
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 8 cut(s) 703, 716, 1541, 1546, 1801, 2266, 2319, 2838
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 9 cut(s) 115, 493, 830, 1186, 1189, 1192, 1436, 1607, 2517
ApoI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
Asp700I GAANNNNTTC 3 cut(s) 1321, 1324, 2141
Asp718I GGTACC 1 cut(s) 866
AspLEI GCGC 5 cut(s) 120, 400, 979, 2784, 3021
AspS9I GGNCC 5 cut(s) 704, 902, 1129, 1541, 1567
AsuC2I CCSGG 1 cut(s) 1441
AsuHPI GGTGA 5 cut(s) 476, 482, 752, 1514, 2806
AsuII TTCGAA 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 1703
AvaII GGWCC 3 cut(s) 902, 1129, 1567
AxyI CCTNAGG 1 cut(s) 2775
BaeGI GKGCMC 1 cut(s) 178
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 1 cut(s) 2268
BamHI GGATCC 1 cut(s) 1859
BanI GGYRCC 1 cut(s) 866
BarI GAAGNNNNNNTAC 2 cut(s) 1718, 1750
BbrPI CACGTG 1 cut(s) 839
BbsI GAAGAC 4 cut(s) 411, 950, 1100, 1984
Bbv12I GWGCWC 2 cut(s) 178, 1390
BbvI GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BccI CCATC 7 cut(s) 461, 727, 1421, 1579, 1801, 2366, 2514
BceAI ACGGC 2 cut(s) 711, 2505
BciT130I CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BclI TGATCA 6 cut(s) 531, 682, 1771, 2050, 2398, 2899
BcnI CCSGG 1 cut(s) 1441
BcoDI GTCTC 3 cut(s) 1119, 1668, 2882
BfaI CTAG 4 cut(s) 6, 38, 1314, 1623
BfmI CTRYAG 4 cut(s) 668, 1089, 1187, 2121
BfoI RGCGCY 2 cut(s) 980, 2785
BfrI CTTAAG 1 cut(s) 1066
BfuAI ACCTGC 1 cut(s) 1475
BglII AGATCT 1 cut(s) 1085
BisI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
BlsI GCNGC 9 cut(s) 117, 495, 832, 1188, 1191, 1194, 1438, 1609, 2519
Bme1390I CCNGG 6 cut(s) 1397, 1413, 1441, 1545, 1821, 1945
Bme18I GGWCC 3 cut(s) 902, 1129, 1567
BmeT110I CYCGRG 1 cut(s) 1703
BmgBI CACGTC 1 cut(s) 2647
BmgT120I GGNCC 5 cut(s) 704, 902, 1129, 1541, 1567
BmiI GGNNCC 5 cut(s) 706, 868, 1569, 1861, 2812
BmrFI CCNGG 6 cut(s) 1397, 1413, 1441, 1545, 1821, 1945
BmrI ACTGGG 2 cut(s) 866, 1326
BmsI GCATC 8 cut(s) 67, 899, 1298, 1390, 1471, 1945, 1994, 2793
BmuI ACTGGG 2 cut(s) 866, 1326
BoxI GACNNNNGTC 1 cut(s) 574
BpiI GAAGAC 4 cut(s) 411, 950, 1100, 1984
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 4 cut(s) 456, 918, 2906, 3030
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 4 cut(s) 116, 2430, 2672, 2930
BpuMI CCSGG 1 cut(s) 1441
BsaAI YACGTR 2 cut(s) 839, 2056
BsaBI GATNNNNATC 2 cut(s) 774, 3031
BsaHI GRCGYC 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 2882
BsaJI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BsaWI WCCGGW 2 cut(s) 824, 1862
Bsc4I CCNNNNNNNGG 8 cut(s) 629, 714, 813, 968, 1799, 1856, 2481, 2791
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 8 cut(s) 619, 779, 872, 1332, 1498, 2269, 2785, 3013
Bse21I CCTNAGG 1 cut(s) 2775
Bse3DI GCAATG 1 cut(s) 2831
Bse8I GATNNNNATC 2 cut(s) 774, 3031
BseAI TCCGGA 1 cut(s) 1862
BseBI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BseDI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BseGI GGATG 9 cut(s) 307, 685, 775, 1405, 1815, 2471, 2506, 2587, 3032
BseJI GATNNNNATC 2 cut(s) 774, 3031
BseLI CCNNNNNNNGG 8 cut(s) 629, 714, 813, 968, 1799, 1856, 2481, 2791
BseMI GCAATG 1 cut(s) 2831
BseNI ACTGG 8 cut(s) 619, 779, 872, 1332, 1498, 2269, 2785, 3013
BseRI GAGGAG 1 cut(s) 2792
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BseYI CCCAGC 1 cut(s) 496
BsgI GTGCAG 3 cut(s) 492, 1017, 2894
BshFI GGCC 8 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321, 2840
BshNI GGYRCC 1 cut(s) 866
BsiHKAI GWGCWC 2 cut(s) 178, 1390
BsiHKCI CYCGRG 1 cut(s) 1703
BsiSI CCGG 4 cut(s) 759, 825, 1440, 1863
BslFI GGGAC 3 cut(s) 260, 1729, 2426
BslI CCNNNNNNNGG 8 cut(s) 629, 714, 813, 968, 1799, 1856, 2481, 2791
BsmAI GTCTC 3 cut(s) 1119, 1668, 2882
BsmFI GGGAC 3 cut(s) 260, 1729, 2426
BsmI GAATGC 3 cut(s) 520, 858, 2604
BsnI GGCC 8 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321, 2840
Bso31I GGTCTC 1 cut(s) 2882
BsoBI CYCGRG 1 cut(s) 1703
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 178, 1390
Bsp13I TCCGGA 1 cut(s) 1862
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 3 cut(s) 179, 365, 1897
BspANI GGCC 8 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321, 2840
BspEI TCCGGA 1 cut(s) 1862
BspHI TCATGA 2 cut(s) 747, 2382
BspLI GGNNCC 5 cut(s) 706, 868, 1569, 1861, 2812
BspMAI CTGCAG 2 cut(s) 1093, 1191
BspMI ACCTGC 1 cut(s) 1475
BspPI GGATC 7 cut(s) 202, 764, 1854, 1867, 2349, 2625, 2846
BspQI GCTCTTC 1 cut(s) 967
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1066
BspTNI GGTCTC 1 cut(s) 2882
BsrDI GCAATG 1 cut(s) 2831
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 8 cut(s) 619, 779, 872, 1332, 1498, 2269, 2785, 3013
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BssNI GRCGYC 1 cut(s) 575
BssT1I CCWWGG 3 cut(s) 862, 1804, 2322
Bst2UI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
Bst4CI ACNGT 7 cut(s) 187, 1172, 1283, 1734, 2122, 2499, 2676
Bst6I CTCTTC 3 cut(s) 644, 967, 2420
BstACI GRCGYC 1 cut(s) 575
BstAFI CTTAAG 1 cut(s) 1066
BstAPI GCANNNNNTGC 3 cut(s) 515, 1613, 1715
BstBAI YACGTR 2 cut(s) 839, 2056
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 5 cut(s) 129, 1023, 2167, 2697, 2838
BstDSI CCRYGG 1 cut(s) 862
BstF5I GGATG 9 cut(s) 307, 685, 775, 1405, 1815, 2471, 2506, 2587, 3032
BstH2I RGCGCY 2 cut(s) 980, 2785
BstHHI GCGC 5 cut(s) 120, 400, 979, 2784, 3021
BstMAI GTCTC 3 cut(s) 1119, 1668, 2882
BstNI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BstNSI RCATGY 3 cut(s) 1304, 1616, 1985
BstPAI GACNNNNGTC 1 cut(s) 574
BstSCI CCNGG 6 cut(s) 1395, 1411, 1439, 1543, 1819, 1943
BstSFI CTRYAG 4 cut(s) 668, 1089, 1187, 2121
BstSLI GKGCMC 1 cut(s) 178
BstV1I GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BstV2I GAAGAC 4 cut(s) 411, 950, 1100, 1984
BstX2I RGATCY 3 cut(s) 1085, 1859, 2851
BstXI CCANNNNNNTGG 2 cut(s) 2264, 2513
BstYI RGATCY 3 cut(s) 1085, 1859, 2851
Bsu36I CCTNAGG 1 cut(s) 2775
BsuRI GGCC 8 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321, 2840
BtgI CCRYGG 1 cut(s) 862
BtrI CACGTC 1 cut(s) 2647
BtsCI GGATG 9 cut(s) 307, 685, 775, 1405, 1815, 2471, 2506, 2587, 3032
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 7 cut(s) 178, 1148, 1836, 1929, 2118, 2495, 2814
BveI ACCTGC 1 cut(s) 1475
Cac8I GCNNGC 5 cut(s) 129, 1023, 2167, 2697, 2838
CaiI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
CciI TCATGA 2 cut(s) 747, 2382
CfoI GCGC 5 cut(s) 120, 400, 979, 2784, 3021
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 5 cut(s) 704, 902, 1129, 1541, 1567
CseI GACGC 1 cut(s) 2572
Csp6I GTAC 6 cut(s) 325, 867, 1735, 2070, 2226, 2959
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviQI GTAC 6 cut(s) 325, 867, 1735, 2070, 2226, 2959
EaeI YGGCCR 1 cut(s) 2266
Eam1104I CTCTTC 3 cut(s) 644, 967, 2420
EarI CTCTTC 3 cut(s) 644, 967, 2420
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 3 cut(s) 862, 1804, 2322
Eco147I AGGCCT 1 cut(s) 2321
Eco31I GGTCTC 1 cut(s) 2882
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 3 cut(s) 902, 1129, 1567
Eco47III AGCGCT 1 cut(s) 978
Eco57I CTGAAG 4 cut(s) 309, 1094, 1341, 1673
Eco72I CACGTG 1 cut(s) 839
Eco81I CCTNAGG 1 cut(s) 2775
Eco88I CYCGRG 1 cut(s) 1703
EcoO109I RGGNCCY 3 cut(s) 704, 902, 1541
EcoRI GAATTC 3 cut(s) 226, 751, 2063
EcoRII CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 3 cut(s) 862, 1804, 2322
EcoT22I ATGCAT 4 cut(s) 567, 1291, 1987, 2606
ErhI CCWWGG 3 cut(s) 862, 1804, 2322
FalI AAGNNNNNCTT 4 cut(s) 1309, 1341, 2790, 2822
FaqI GGGAC 3 cut(s) 260, 1729, 2426
FauI CCCGC 1 cut(s) 1904
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 6 cut(s) 531, 682, 1771, 2050, 2398, 2899
Fnu4HI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
FokI GGATG 9 cut(s) 294, 672, 762, 1412, 1822, 2458, 2493, 2594, 3019
Fsp4HI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
FspBI CTAG 4 cut(s) 6, 38, 1314, 1623
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 5 cut(s) 119, 399, 978, 2783, 3020
GluI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 4 cut(s) 456, 918, 2906, 3030
HaeII RGCGCY 2 cut(s) 980, 2785
HaeIII GGCC 8 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321, 2840
HapII CCGG 4 cut(s) 759, 825, 1440, 1863
HgaI GACGC 1 cut(s) 2572
HhaI GCGC 5 cut(s) 120, 400, 979, 2784, 3021
Hin1I GRCGYC 1 cut(s) 575
Hin6I GCGC 5 cut(s) 118, 398, 977, 2782, 3019
HinP1I GCGC 5 cut(s) 118, 398, 977, 2782, 3019
HincII GTYRAC 1 cut(s) 2335
HindII GTYRAC 1 cut(s) 2335
HindIII AAGCTT 1 cut(s) 2693
HpaII CCGG 4 cut(s) 759, 825, 1440, 1863
HphI GGTGA 5 cut(s) 476, 482, 752, 1514, 2806
Hpy166II GTNNAC 7 cut(s) 176, 1567, 2335, 2502, 2663, 2961, 2972
Hpy8I GTNNAC 7 cut(s) 176, 1567, 2335, 2502, 2663, 2961, 2972
Hpy99I CGWCG 1 cut(s) 2873
HpyCH4III ACNGT 7 cut(s) 187, 1172, 1283, 1734, 2122, 2499, 2676
HpyCH4IV ACGT 5 cut(s) 575, 838, 2055, 2646, 2868
HpySE526I ACGT 5 cut(s) 575, 838, 2055, 2646, 2868
Hsp92I GRCGYC 1 cut(s) 575
HspAI GCGC 5 cut(s) 118, 398, 977, 2782, 3019
Kpn2I TCCGGA 1 cut(s) 1862
KpnI GGTACC 1 cut(s) 870
Ksp22I TGATCA 6 cut(s) 531, 682, 1771, 2050, 2398, 2899
LguI GCTCTTC 1 cut(s) 967
LmnI GCTCC 4 cut(s) 112, 827, 838, 2779
Lsp1109I GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
LweI GCATC 8 cut(s) 67, 899, 1298, 1390, 1471, 1945, 1994, 2793
MaeI CTAG 4 cut(s) 6, 38, 1314, 1623
MaeII ACGT 5 cut(s) 575, 838, 2055, 2646, 2868
MaeIII GTNAC 3 cut(s) 269, 1502, 2491
MflI RGATCY 3 cut(s) 1085, 1859, 2851
MhlI GDGCHC 2 cut(s) 178, 1390
MlsI TGGCCA 1 cut(s) 2268
MluNI TGGCCA 1 cut(s) 2268
MlyI GAGTC 4 cut(s) 2549, 2728, 2779, 2914
MmeI TCCRAC 1 cut(s) 1837
Mox20I TGGCCA 1 cut(s) 2268
Mph1103I ATGCAT 4 cut(s) 567, 1291, 1987, 2606
MroI TCCGGA 1 cut(s) 1862
MroXI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
MscI TGGCCA 1 cut(s) 2268
MseI TTAA 5 cut(s) 1067, 1586, 1751, 1902, 2931
MslI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2550
Msp20I TGGCCA 1 cut(s) 2268
MspA1I CMGCKG 1 cut(s) 496
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 4 cut(s) 759, 825, 1440, 1863
MspR9I CCNGG 6 cut(s) 1397, 1413, 1441, 1545, 1821, 1945
Mva1269I GAATGC 3 cut(s) 520, 858, 2604
MvaI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
NciI CCSGG 1 cut(s) 1441
NcoI CCATGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 1798
NlaIV GGNNCC 5 cut(s) 706, 868, 1569, 1861, 2812
NmuCI GTSAC 2 cut(s) 1502, 2491
NsbI TGCGCA 1 cut(s) 399
NsiI ATGCAT 4 cut(s) 567, 1291, 1987, 2606
NspI RCATGY 3 cut(s) 1304, 1616, 1985
NspV TTCGAA 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 1703
PagI TCATGA 2 cut(s) 747, 2382
PceI AGGCCT 1 cut(s) 2321
PciSI GCTCTTC 1 cut(s) 967
PctI GAATGC 3 cut(s) 520, 858, 2604
PdmI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
PflMI CCANNNNNTGG 2 cut(s) 1799, 2791
PfoI TCCNGGA 1 cut(s) 1819
PkrI GCNGC 9 cut(s) 117, 495, 832, 1188, 1191, 1194, 1438, 1609, 2519
PleI GAGTC 4 cut(s) 2549, 2727, 2778, 2913
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 4 cut(s) 2549, 2727, 2778, 2913
Ppu21I YACGTR 2 cut(s) 839, 2056
PpuMI RGGWCCY 1 cut(s) 902
PshAI GACNNNNGTC 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
PspCI CACGTG 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
PspN4I GGNNCC 5 cut(s) 706, 868, 1569, 1861, 2812
PspPI GGNCC 5 cut(s) 704, 902, 1129, 1541, 1567
PspPPI RGGWCCY 1 cut(s) 902
PspXI VCTCGAGB 1 cut(s) 1703
PstI CTGCAG 2 cut(s) 1093, 1191
PstNI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
PsuI RGATCY 3 cut(s) 1085, 1859, 2851
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 6 cut(s) 326, 868, 1736, 2071, 2227, 2960
RsaNI GTAC 6 cut(s) 325, 867, 1735, 2070, 2226, 2959
RseI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2550
SapI GCTCTTC 1 cut(s) 967
SaqAI TTAA 5 cut(s) 1067, 1586, 1751, 1902, 2931
SatI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
Sau96I GGNCC 5 cut(s) 704, 902, 1129, 1541, 1567
SchI GAGTC 4 cut(s) 2549, 2728, 2779, 2914
ScrFI CCNGG 6 cut(s) 1397, 1413, 1441, 1545, 1821, 1945
SduI GDGCHC 2 cut(s) 178, 1390
SfaNI GCATC 8 cut(s) 67, 899, 1298, 1390, 1471, 1945, 1994, 2793
SfcI CTRYAG 4 cut(s) 668, 1089, 1187, 2121
Sfr274I CTCGAG 1 cut(s) 1703
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 3 cut(s) 902, 1129, 1567
SlaI CTCGAG 1 cut(s) 1703
SmiMI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2550
SmlI CTYRAG 6 cut(s) 95, 1066, 1703, 2445, 2651, 2909
SmoI CTYRAG 6 cut(s) 95, 1066, 1703, 2445, 2651, 2909
SseBI AGGCCT 1 cut(s) 2321
SsiI CCGC 3 cut(s) 179, 365, 1897
SspI AATATT 1 cut(s) 2142
SspMI CTAG 4 cut(s) 6, 38, 1314, 1623
StuI AGGCCT 1 cut(s) 2321
StyD4I CCNGG 6 cut(s) 1395, 1411, 1439, 1543, 1819, 1943
StyI CCWWGG 3 cut(s) 862, 1804, 2322
TaaI ACNGT 7 cut(s) 187, 1172, 1283, 1734, 2122, 2499, 2676
TaiI ACGT 5 cut(s) 578, 841, 2058, 2649, 2871
TaqI TCGA 6 cut(s) 602, 1419, 1479, 1704, 2109, 2370
TatI WGTACW 1 cut(s) 2958
Tru1I TTAA 5 cut(s) 1067, 1586, 1751, 1902, 2931
Tru9I TTAA 5 cut(s) 1067, 1586, 1751, 1902, 2931
TscAI CASTG 7 cut(s) 178, 1148, 1836, 1929, 2125, 2502, 2821
TseFI GTSAC 2 cut(s) 1502, 2491
TseI GCWGC 9 cut(s) 115, 493, 830, 1186, 1189, 1192, 1436, 1607, 2517
Tsp45I GTSAC 2 cut(s) 1502, 2491
TspGWI ACGGA 2 cut(s) 1521, 2384
TspRI CASTG 7 cut(s) 178, 1148, 1836, 1929, 2125, 2502, 2821
Van91I CCANNNNNTGG 2 cut(s) 1799, 2791
Vha464I CTTAAG 1 cut(s) 1066
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 902, 1129, 1567
XapI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
XceI RCATGY 3 cut(s) 1304, 1616, 1985
XhoI CTCGAG 1 cut(s) 1703
XmnI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
XspI CTAG 4 cut(s) 6, 38, 1314, 1623
ZraI GACGTC 1 cut(s) 576
Zsp2I ATGCAT 4 cut(s) 567, 1291, 1987, 2606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.