Rroxscaffold_4G00293720

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007939
Physical Location & Seq
Reverse (-)
13917633 .. 13923844
6212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293720.1

Sequence Viewer

Length: 2307 bp
ATGAATTCAACCGGCATGGCTGATCCACATCCAGTAGGGTTGTGTAATGGAGTGGATTCCATAGAGGGGTTGCACACCGGAGCAGCTCCACGTGGCTTGATAGGAATGCAGAGCCATGGTACCCCAGTTTCTCATTCATTTGCATCTGCTGGAGGTCTTTCTTTGTCAAGAAGCAGAACCCCTGAACCACAATTATTTGGAAGTTCTGGTCTTCCTCCTGTGGGAAGAGCGTTTCCTAATGTTGCTGGTGCGGATATCCAAAATGATAGTTCGCCTGCCATGACTGAGTTGCCCAATATGACAACAAATTTATCTGGCTTAAGCCTCTCAAAAGTTAGATCTGCAGATGAAGATAGTCGTCTTCAGTCTCAGCTTCGTATGGACCTTCATAATCAGTGTGATTTTCTGTTCAACACACCAAACAGTCAGAATCAGAGGCTGCAGCAGCAACTGATAGAGAAGTCAAATGCTGAAAACTTTTCGCTTTCTACAAATTATGCTCATTTAGCAAACAAAAATGGGATTCTAACAAACCGTAATGCATCAAATGGGCATGTTCATTTTCCTAGAAGAATTTCTTCTCCCAGTCTTTACTCAAACGGGAGTTCTTCAGGACTTGGAACTTTGGAAGGGTTGAATGCTCATTACCAGGATGCAAATACCCCAGGAATCGACTTTGATGGTCATGCTGCCGGGGCATATCCTGTAAATCAGAAGTTGAACAATCATCTTGATGCAGGTTTTACTGGTAGTGGTGACGGACAAAGTATGAACAGATTAGGGAACAAAGTAGGACCTGGCCTTCATTCTTCGTTTGTGGACCCATCATATATTCAGTTAATGCAAAGAACTGATTATGCAGCACATGCTTTGGCTAGTCCAAGTGGTTATTCTCTGAGTAAAAATAACTTTGGCAATTTGCATGGAGACTTAGAGGGGCTTCAGAAAGCATACCTCGAGGCAATACTTGCTCAAAGGAAGCAACAGTACGAGTTGTCCCTTTTAAGTCAATCTGGGGGGTTTGATCATGGTTATTTTGGGAATCCATCATATGGCCTGGGGATGACATATCCAGGAATCTCAGTGGCAAACTCTGTTCTTCCTTCTGTTGGATCCGGAAGTTCAATGTTTCAGAATGAGAAGATTTCCCGCTTTAATTCTATGATGAGAAGTTCAGCGGGAGGTTCAGTCAGCACTTGGCACTCAGATGCTGGAAATGACTTAGAAGGAAGACACGCATCATCATTATTAGATGAGTTCAAGAACAACAAGAATAAGTCTTTTGAACTTGCAGATATTGTTGATCACGTAGTTGAATTCAGTACCGATCAGTATGGAAGTCGCTTTATCCAGCAGAAGCTCGAAACTGCCACTGCAGAAGAAAAAATGAAAATATTCCCTGAGATTATTCCTCATGCTCGCACCTTGATGACTGATGTTTTTGGAAATTATGTCATACAGAAATTCTTTGAGCATGGTACAGAAAGCCAAAGAAATGAATTGACGAACCAACTTACTGGCCATGTTTTGCCTCTCAGTCTGCAAATGTATGGTTGTAGAGTGATTCAGAAGGCCTTGGAAATGGTTGACAAGGATCATCAGGCTCAAATGGTGTCAGAACTCGATGGTTCAGTCATGAAATGTGTCCGTGATCAAAATGGGAATCATGTCATTCAGAAGAGTATAGAATGTGTCCCTCAAGATCAAATTCAGTTCATCATTTCATCCTTCTATGGGCAAGTTGTCACACTGTCCACCCATCCTTATGGCTGCCGTGTCATACAGAGGGTTCTGGAACATTGTGATGACTCAAAGACGCAAGAAATTATCATGGATGAAGTCATGCAATCTGTATGCATTCTGGCACAGGATCAGTATGGTAATTATGTTATTCAGCACGTCCTTGAACATGGTAAACCACATGAACGATCTGCAATTATTCACAAGCTTGCTGGACAAATTGTAAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAAAAGTGCTTGACTTTTGGGAGTCCTGAGGAGCGCCAGTTGTTGGTGAAAGAGATGCTTGGTTCCACTGATGAAAATGAGCCGCTGCAGGCCATGATGAAAGATCCATTTGGAAACTACGTTGTTCAGAAGGTTCTGGAGACCTGTGATGATCAGAGTCTTGAGTTGATTGTTTCTCGCATTAAGGTTCATTTGACTGCCTTGAAGAAGTACACCTATGGTAAACATATTGTTTCCCGTGTTGAGAAACTCATCACAACTGGAGAAAGGCGCATAGGATTGTCATCCTCAGTTTCGTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

768

Amino Acids

84.12

Weight (kDa)

6.17

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 68 - 431 1.6e-105 Nucleic acid binding protein NABP
PUF PF00806 436 - 470 7.4e-12 Pumilio-family RNA binding repeat
PUF PF00806 474 - 505 1.3e-08 Pumilio-family RNA binding repeat
PUF PF00806 508 - 541 9.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 580 - 614 5.6e-13 Pumilio-family RNA binding repeat
PUF PF00806 618 - 650 2e-09 Pumilio-family RNA binding repeat
PUF PF00806 653 - 686 1.3e-08 Pumilio-family RNA binding repeat
PUF PF00806 699 - 728 3.3e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 956
Acc36I ACCTGC 1 cut(s) 728
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 2 cut(s) 1052, 2044
AccIII TCCGGA 1 cut(s) 1115
AciI CCGC 4 cut(s) 251, 1150, 1178, 2084
AclWI GGATC 6 cut(s) 17, 1107, 1120, 1602, 1878, 2099
AcoI YGGCCR 1 cut(s) 1519
AcsI RAATTY 6 cut(s) 4, 307, 573, 1316, 1463, 1707
AcuI CTGAAG 3 cut(s) 347, 594, 926
AcvI CACGTG 1 cut(s) 92
AfaI GTAC 5 cut(s) 121, 989, 1324, 1480, 2213
AfiI CCNNNNNNNGG 6 cut(s) 66, 221, 1052, 1109, 1734, 2044
AflII CTTAAG 1 cut(s) 319
AjiI CACGTC 1 cut(s) 1900
AjnI CCWGG 5 cut(s) 648, 664, 796, 1056, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 874, 906
AluBI AGCT 4 cut(s) 86, 373, 1360, 1948
AluI AGCT 4 cut(s) 86, 373, 1360, 1948
Alw26I GTCTC 3 cut(s) 372, 921, 2135
AlwI GGATC 6 cut(s) 17, 1107, 1120, 1602, 1878, 2099
AlwNI CAGNNNCTG 3 cut(s) 439, 451, 1211
Ama87I CYCGRG 1 cut(s) 956
Aor13HI TCCGGA 1 cut(s) 1115
AoxI GGCC 5 cut(s) 799, 1054, 1519, 1572, 2091
ApeKI GCWGC 8 cut(s) 83, 439, 442, 445, 689, 860, 1770, 2086
ApoI RAATTY 6 cut(s) 4, 307, 573, 1316, 1463, 1707
Asp700I GAANNNNTTC 3 cut(s) 574, 577, 1394
Asp718I GGTACC 1 cut(s) 119
AspLEI GCGC 2 cut(s) 2037, 2274
AspS9I GGNCC 3 cut(s) 382, 794, 820
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 2 cut(s) 767, 2059
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 3 cut(s) 382, 794, 820
AxyI CCTNAGG 1 cut(s) 2028
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 1 cut(s) 1521
BamHI GGATCC 1 cut(s) 1112
BanI GGYRCC 1 cut(s) 119
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbrPI CACGTG 1 cut(s) 92
BbsI GAAGAC 4 cut(s) 203, 353, 1237, 2292
BbvI GCAGC 8 cut(s) 95, 426, 454, 457, 676, 872, 1757, 2073
BccI CCATC 5 cut(s) 674, 832, 1054, 1619, 1767
BceAI ACGGC 1 cut(s) 1758
BciT130I CCWGG 5 cut(s) 650, 666, 798, 1058, 1074
BclI TGATCA 4 cut(s) 1024, 1303, 1651, 2152
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 3 cut(s) 372, 921, 2135
BfaI CTAG 2 cut(s) 567, 876
BfmI CTRYAG 4 cut(s) 342, 440, 1374, 2087
BfoI RGCGCY 1 cut(s) 2038
BfrI CTTAAG 1 cut(s) 319
BfuAI ACCTGC 1 cut(s) 728
BglII AGATCT 1 cut(s) 338
BisI GCNGC 9 cut(s) 84, 440, 443, 446, 690, 861, 1771, 2084, 2087
BlsI GCNGC 9 cut(s) 85, 441, 444, 447, 691, 862, 1772, 2085, 2088
Bme1390I CCNGG 6 cut(s) 650, 666, 694, 798, 1058, 1074
Bme18I GGWCC 3 cut(s) 382, 794, 820
BmeT110I CYCGRG 1 cut(s) 956
BmgBI CACGTC 1 cut(s) 1900
BmgT120I GGNCC 3 cut(s) 382, 794, 820
BmiI GGNNCC 4 cut(s) 121, 822, 1114, 2065
BmrFI CCNGG 6 cut(s) 650, 666, 694, 798, 1058, 1074
BmrI ACTGGG 2 cut(s) 119, 579
BmsI GCATC 7 cut(s) 152, 551, 643, 724, 1198, 1247, 2046
BmuI ACTGGG 2 cut(s) 119, 579
BpiI GAAGAC 4 cut(s) 203, 353, 1237, 2292
BpmI CTGGAG 3 cut(s) 171, 2159, 2283
BpuEI CTTGAG 2 cut(s) 1683, 2183
BpuMI CCSGG 1 cut(s) 694
BsaAI YACGTR 2 cut(s) 92, 1309
BsaBI GATNNNNATC 2 cut(s) 27, 2284
BsaI GGTCTC 1 cut(s) 2135
BsaJI CCNNGG 5 cut(s) 115, 664, 693, 1057, 1575
BsaWI WCCGGW 2 cut(s) 77, 1115
BsaXI ACNNNNNCTCC 4 cut(s) 1173, 1203, 2024, 2054
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 221, 1052, 1109, 1734, 2044
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 7 cut(s) 32, 125, 585, 751, 1522, 2038, 2266
Bse21I CCTNAGG 1 cut(s) 2028
Bse8I GATNNNNATC 2 cut(s) 27, 2284
BseAI TCCGGA 1 cut(s) 1115
BseBI CCWGG 5 cut(s) 650, 666, 798, 1058, 1074
BseDI CCNNGG 5 cut(s) 115, 664, 693, 1057, 1575
BseGI GGATG 7 cut(s) 28, 658, 1068, 1724, 1759, 1840, 2285
BseJI GATNNNNATC 2 cut(s) 27, 2284
BseLI CCNNNNNNNGG 6 cut(s) 66, 221, 1052, 1109, 1734, 2044
BseMII CTCAG 9 cut(s) 276, 383, 887, 1095, 1218, 1392, 1549, 2019, 2304
BseNI ACTGG 7 cut(s) 32, 125, 585, 751, 1522, 2038, 2266
BseRI GAGGAG 1 cut(s) 2045
BseXI GCAGC 8 cut(s) 95, 426, 454, 457, 676, 872, 1757, 2073
BshFI GGCC 5 cut(s) 801, 1056, 1521, 1574, 2093
BshNI GGYRCC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 956
BsiSI CCGG 4 cut(s) 12, 78, 693, 1116
BslFI GGGAC 2 cut(s) 982, 1679
BslI CCNNNNNNNGG 6 cut(s) 66, 221, 1052, 1109, 1734, 2044
BsmAI GTCTC 3 cut(s) 372, 921, 2135
BsmFI GGGAC 2 cut(s) 982, 1679
BsmI GAATGC 3 cut(s) 111, 643, 1857
BsnI GGCC 5 cut(s) 801, 1056, 1521, 1574, 2093
Bso31I GGTCTC 1 cut(s) 2135
BsoBI CYCGRG 1 cut(s) 956
Bsp13I TCCGGA 1 cut(s) 1115
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 4 cut(s) 251, 1150, 1178, 2084
BspANI GGCC 5 cut(s) 801, 1056, 1521, 1574, 2093
BspCNI CTCAG 9 cut(s) 277, 382, 888, 1094, 1217, 1393, 1548, 2020, 2303
BspEI TCCGGA 1 cut(s) 1115
BspHI TCATGA 1 cut(s) 1635
BspLI GGNNCC 4 cut(s) 121, 822, 1114, 2065
BspMAI CTGCAG 4 cut(s) 346, 444, 1378, 2091
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 6 cut(s) 17, 1107, 1120, 1602, 1878, 2099
BspQI GCTCTTC 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 2135
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 7 cut(s) 32, 125, 585, 751, 1522, 2038, 2266
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 5 cut(s) 115, 664, 693, 1057, 1575
BssT1I CCWWGG 2 cut(s) 115, 1575
Bst2UI CCWGG 5 cut(s) 650, 666, 798, 1058, 1074
Bst4CI ACNGT 4 cut(s) 425, 536, 987, 1752
Bst6I CTCTTC 2 cut(s) 220, 1673
BstAFI CTTAAG 1 cut(s) 319
BstAPI GCANNNNNTGC 2 cut(s) 866, 968
BstBAI YACGTR 2 cut(s) 92, 1309
BstC8I GCNNGC 4 cut(s) 276, 1420, 1950, 2091
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 7 cut(s) 28, 658, 1068, 1724, 1759, 1840, 2285
BstH2I RGCGCY 1 cut(s) 2038
BstHHI GCGC 2 cut(s) 2037, 2274
BstMAI GTCTC 3 cut(s) 372, 921, 2135
BstMWI GCNNNNNNNGC 5 cut(s) 445, 506, 695, 866, 968
BstNI CCWGG 5 cut(s) 650, 666, 798, 1058, 1074
BstNSI RCATGY 2 cut(s) 557, 869
BstSCI CCNGG 6 cut(s) 648, 664, 692, 796, 1056, 1072
BstSFI CTRYAG 4 cut(s) 342, 440, 1374, 2087
BstV1I GCAGC 8 cut(s) 95, 426, 454, 457, 676, 872, 1757, 2073
BstV2I GAAGAC 4 cut(s) 203, 353, 1237, 2292
BstX2I RGATCY 3 cut(s) 338, 1112, 2104
BstXI CCANNNNNNTGG 2 cut(s) 1517, 1766
BstYI RGATCY 3 cut(s) 338, 1112, 2104
Bsu36I CCTNAGG 1 cut(s) 2028
BsuRI GGCC 5 cut(s) 801, 1056, 1521, 1574, 2093
BtgI CCRYGG 1 cut(s) 115
BtrI CACGTC 1 cut(s) 1900
BtsCI GGATG 7 cut(s) 28, 658, 1068, 1724, 1759, 1840, 2285
BtsI GCAGTG 1 cut(s) 1371
BtsIMutI CAGTG 5 cut(s) 401, 1089, 1371, 1748, 2067
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 4 cut(s) 276, 1420, 1950, 2091
CaiI CAGNNNCTG 3 cut(s) 439, 451, 1211
CciI TCATGA 1 cut(s) 1635
CfoI GCGC 2 cut(s) 2037, 2274
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 382, 794, 820
CseI GACGC 1 cut(s) 1825
Csp6I GTAC 5 cut(s) 120, 988, 1323, 1479, 2212
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviQI GTAC 5 cut(s) 120, 988, 1323, 1479, 2212
EaeI YGGCCR 1 cut(s) 1519
Eam1104I CTCTTC 2 cut(s) 220, 1673
EarI CTCTTC 2 cut(s) 220, 1673
Eco130I CCWWGG 2 cut(s) 115, 1575
Eco147I AGGCCT 1 cut(s) 1574
Eco31I GGTCTC 1 cut(s) 2135
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 382, 794, 820
Eco57I CTGAAG 3 cut(s) 347, 594, 926
Eco72I CACGTG 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 2028
Eco88I CYCGRG 1 cut(s) 956
EcoO109I RGGNCCY 1 cut(s) 794
EcoRI GAATTC 2 cut(s) 4, 1316
EcoRII CCWGG 5 cut(s) 648, 664, 796, 1056, 1072
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 115, 1575
EcoT22I ATGCAT 2 cut(s) 544, 1859
ErhI CCWWGG 2 cut(s) 115, 1575
FalI AAGNNNNNCTT 4 cut(s) 562, 594, 2043, 2075
FaqI GGGAC 2 cut(s) 982, 1679
FauI CCCGC 2 cut(s) 1157, 1171
FauNDI CATATG 1 cut(s) 1051
FbaI TGATCA 4 cut(s) 1024, 1303, 1651, 2152
Fnu4HI GCNGC 9 cut(s) 84, 440, 443, 446, 690, 861, 1771, 2084, 2087
FokI GGATG 7 cut(s) 15, 665, 1075, 1711, 1746, 1847, 2272
Fsp4HI GCNGC 9 cut(s) 84, 440, 443, 446, 690, 861, 1771, 2084, 2087
FspBI CTAG 2 cut(s) 567, 876
GlaI GCGC 2 cut(s) 2036, 2273
GluI GCNGC 9 cut(s) 84, 440, 443, 446, 690, 861, 1771, 2084, 2087
GsuI CTGGAG 3 cut(s) 171, 2159, 2283
HaeII RGCGCY 1 cut(s) 2038
HaeIII GGCC 5 cut(s) 801, 1056, 1521, 1574, 2093
HapII CCGG 4 cut(s) 12, 78, 693, 1116
HgaI GACGC 1 cut(s) 1825
HhaI GCGC 2 cut(s) 2037, 2274
Hin6I GCGC 2 cut(s) 2035, 2272
HinP1I GCGC 2 cut(s) 2035, 2272
HincII GTYRAC 1 cut(s) 1588
HindII GTYRAC 1 cut(s) 1588
HindIII AAGCTT 1 cut(s) 1946
HpaII CCGG 4 cut(s) 12, 78, 693, 1116
HphI GGTGA 2 cut(s) 767, 2059
Hpy166II GTNNAC 6 cut(s) 820, 1588, 1755, 1916, 2214, 2225
Hpy8I GTNNAC 6 cut(s) 820, 1588, 1755, 1916, 2214, 2225
HpyAV CCTTC 8 cut(s) 395, 623, 812, 1113, 1220, 1564, 1738, 2125
HpyCH4III ACNGT 4 cut(s) 425, 536, 987, 1752
HpyCH4IV ACGT 4 cut(s) 91, 1308, 1899, 2121
HpyF10VI GCNNNNNNNGC 5 cut(s) 445, 506, 695, 866, 968
HpySE526I ACGT 4 cut(s) 91, 1308, 1899, 2121
HspAI GCGC 2 cut(s) 2035, 2272
Kpn2I TCCGGA 1 cut(s) 1115
KpnI GGTACC 1 cut(s) 123
Ksp22I TGATCA 4 cut(s) 1024, 1303, 1651, 2152
LguI GCTCTTC 1 cut(s) 220
LmnI GCTCC 3 cut(s) 80, 91, 2032
Lsp1109I GCAGC 8 cut(s) 95, 426, 454, 457, 676, 872, 1757, 2073
LweI GCATC 7 cut(s) 152, 551, 643, 724, 1198, 1247, 2046
MaeI CTAG 2 cut(s) 567, 876
MaeII ACGT 4 cut(s) 91, 1308, 1899, 2121
MaeIII GTNAC 2 cut(s) 755, 1744
MflI RGATCY 3 cut(s) 338, 1112, 2104
MlsI TGGCCA 1 cut(s) 1521
MluNI TGGCCA 1 cut(s) 1521
MlyI GAGTC 4 cut(s) 1802, 1981, 2032, 2167
MmeI TCCRAC 1 cut(s) 1090
Mox20I TGGCCA 1 cut(s) 1521
Mph1103I ATGCAT 2 cut(s) 544, 1859
MroI TCCGGA 1 cut(s) 1115
MroXI GAANNNNTTC 3 cut(s) 574, 577, 1394
MscI TGGCCA 1 cut(s) 1521
MseI TTAA 5 cut(s) 320, 839, 1004, 1155, 2184
MslI CAYNNNNRTG 5 cut(s) 732, 1206, 1427, 1764, 1803
Msp20I TGGCCA 1 cut(s) 1521
MspA1I CMGCKG 2 cut(s) 1178, 2086
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 4 cut(s) 12, 78, 693, 1116
MspR9I CCNGG 6 cut(s) 650, 666, 694, 798, 1058, 1074
Mva1269I GAATGC 3 cut(s) 111, 643, 1857
MvaI CCWGG 5 cut(s) 650, 666, 798, 1058, 1074
MwoI GCNNNNNNNGC 5 cut(s) 445, 506, 695, 866, 968
NciI CCSGG 1 cut(s) 694
NcoI CCATGG 1 cut(s) 115
NdeI CATATG 1 cut(s) 1051
NlaIV GGNNCC 4 cut(s) 121, 822, 1114, 2065
NmuCI GTSAC 2 cut(s) 755, 1744
NsiI ATGCAT 2 cut(s) 544, 1859
NspI RCATGY 2 cut(s) 557, 869
PaeR7I CTCGAG 1 cut(s) 956
PagI TCATGA 1 cut(s) 1635
PceI AGGCCT 1 cut(s) 1574
PciSI GCTCTTC 1 cut(s) 220
PctI GAATGC 3 cut(s) 111, 643, 1857
PdmI GAANNNNTTC 3 cut(s) 574, 577, 1394
PfeI GAWTC 8 cut(s) 56, 430, 523, 669, 1042, 1077, 1564, 1663
PflMI CCANNNNNTGG 2 cut(s) 1052, 2044
PfoI TCCNGGA 1 cut(s) 1072
PkrI GCNGC 9 cut(s) 85, 441, 444, 447, 691, 862, 1772, 2085, 2088
PleI GAGTC 4 cut(s) 1802, 1980, 2031, 2166
PmaCI CACGTG 1 cut(s) 92
PmlI CACGTG 1 cut(s) 92
PpsI GAGTC 4 cut(s) 1802, 1980, 2031, 2166
Ppu21I YACGTR 2 cut(s) 92, 1309
PpuMI RGGWCCY 1 cut(s) 794
Psp5II RGGWCCY 1 cut(s) 794
Psp6I CCWGG 5 cut(s) 648, 664, 796, 1056, 1072
PspCI CACGTG 1 cut(s) 92
PspGI CCWGG 5 cut(s) 648, 664, 796, 1056, 1072
PspN4I GGNNCC 4 cut(s) 121, 822, 1114, 2065
PspPI GGNCC 3 cut(s) 382, 794, 820
PspPPI RGGWCCY 1 cut(s) 794
PspXI VCTCGAGB 1 cut(s) 956
PstI CTGCAG 4 cut(s) 346, 444, 1378, 2091
PstNI CAGNNNCTG 3 cut(s) 439, 451, 1211
PsuI RGATCY 3 cut(s) 338, 1112, 2104
RsaI GTAC 5 cut(s) 121, 989, 1324, 1480, 2213
RsaNI GTAC 5 cut(s) 120, 988, 1323, 1479, 2212
RseI CAYNNNNRTG 5 cut(s) 732, 1206, 1427, 1764, 1803
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 5 cut(s) 320, 839, 1004, 1155, 2184
SatI GCNGC 9 cut(s) 84, 440, 443, 446, 690, 861, 1771, 2084, 2087
Sau96I GGNCC 3 cut(s) 382, 794, 820
SchI GAGTC 4 cut(s) 1802, 1981, 2032, 2167
ScrFI CCNGG 6 cut(s) 650, 666, 694, 798, 1058, 1074
SfaNI GCATC 7 cut(s) 152, 551, 643, 724, 1198, 1247, 2046
SfcI CTRYAG 4 cut(s) 342, 440, 1374, 2087
Sfr274I CTCGAG 1 cut(s) 956
SinI GGWCC 3 cut(s) 382, 794, 820
SlaI CTCGAG 1 cut(s) 956
SmiMI CAYNNNNRTG 5 cut(s) 732, 1206, 1427, 1764, 1803
SmlI CTYRAG 4 cut(s) 319, 956, 1698, 2162
SmoI CTYRAG 4 cut(s) 319, 956, 1698, 2162
SseBI AGGCCT 1 cut(s) 1574
SsiI CCGC 4 cut(s) 251, 1150, 1178, 2084
SspI AATATT 1 cut(s) 1395
SspMI CTAG 2 cut(s) 567, 876
StuI AGGCCT 1 cut(s) 1574
StyD4I CCNGG 6 cut(s) 648, 664, 692, 796, 1056, 1072
StyI CCWWGG 2 cut(s) 115, 1575
TaaI ACNGT 4 cut(s) 425, 536, 987, 1752
TaiI ACGT 4 cut(s) 94, 1311, 1902, 2124
TaqI TCGA 4 cut(s) 672, 957, 1362, 1623
TatI WGTACW 1 cut(s) 2211
TauI GCSGC 1 cut(s) 2086
TfiI GAWTC 8 cut(s) 56, 430, 523, 669, 1042, 1077, 1564, 1663
Tru1I TTAA 5 cut(s) 320, 839, 1004, 1155, 2184
Tru9I TTAA 5 cut(s) 320, 839, 1004, 1155, 2184
TscAI CASTG 5 cut(s) 401, 1089, 1378, 1755, 2074
TseFI GTSAC 2 cut(s) 755, 1744
TseI GCWGC 8 cut(s) 83, 439, 442, 445, 689, 860, 1770, 2086
Tsp45I GTSAC 2 cut(s) 755, 1744
TspGWI ACGGA 2 cut(s) 774, 1637
TspRI CASTG 5 cut(s) 401, 1089, 1378, 1755, 2074
Van91I CCANNNNNTGG 2 cut(s) 1052, 2044
Vha464I CTTAAG 1 cut(s) 319
VpaK11BI GGWCC 3 cut(s) 382, 794, 820
XapI RAATTY 6 cut(s) 4, 307, 573, 1316, 1463, 1707
XceI RCATGY 2 cut(s) 557, 869
XhoI CTCGAG 1 cut(s) 956
XmnI GAANNNNTTC 3 cut(s) 574, 577, 1394
XspI CTAG 2 cut(s) 567, 876
Zsp2I ATGCAT 2 cut(s) 544, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.