MD07G1171400.v1.1

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007939
Physical Location & Seq
Forward (+)
24767446 .. 24774315
6870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1171400.v1.1.491

Sequence Viewer

Length: 3048 bp
ATGTTATCAACCCGAGACAATAATCTGCAAGGACCCAATGGGAATTTAGAAGATGCTTTACAGAGTGAGCTTGAATTGATTCTGAGAGAGCAGCGCAATCAGCATGCCATTCATCGGGAGAGGGACATGAATATGTATAGGAGCGGTAGTGCCCCGCCAACTGTTGAGGGATCGCTAACTGCTGTCGGTAGTCTCTTTAGGAACTCAGACTTTAGGGATGTGACTAGTAGGATTAGTAATAACAATGGGATTTTGTCCGAAGATGAGATTCGATCTCATCCTGCTTATCTTTCATACTACTATTCGCATGACAACATTAACCCAAGATTGCCTCCACCGTTGTTGTCAAGAGAGGATTGGCGTGTTGCACAAAGGTTTCAGGGCGGCGGGTCTTCTTTTGAGGGCATTGGAGACTGGAGGAGGAAGAAGTTCGCTGATGATCGTGATAGTTTGTCGCTGTTTGCCACGCAGTTGGGGCTACAAAAGGCAGAGAATGATATGATGGAACTGAGGAATGCTAATGGGAGCAATATCCCTGAACAAACTTCATCTGAGTGGCTTGATAGAAGCTCGGATGGTTTGATTGGTTTGCCGACAACTGGGCTTGGTGGAAGGAGAAAGAGTTTTGCTGACATTTTACAGGAAGGACTTGATCAAACTGCCTCCTTGTCAGGTCCCCTGTCAAGGCCATCAAGTCGTAACGCATTTGGTGATATCATGGATTCCACAGGCATGGCTGATCCCCATCCAGTTGGGTTATCTAATGGAGTGGAGTCTGCAGAGGGTATGCATAATGGAAGAGCTTCATGTGGCCTGGTGGGAGTTCAGAGCCATGGCTCAACAGCTTCTAATTCCTTTGCATCTGCTGTCGGTCCTTCTTTGCCAAGAAGCAGAACCCCTGAACAACAACTACTTGGGAGGTCTAACAGTTTTGGCCTCCCCCCTGTAGGGAGTAGAGTTTTCCCAACCAAGAGGAAGAATGTTGCTAGTCCAGATATTCAAAATGATCGTTCTTCCAGCATGAATGATCTGGCAGATATTTCAGTCAATTTTTCTGGCTTAAGCCTGTCAAAAATTAGACCTGTAGATGATGATAGACGTATTCAGTCGCAGCTTCATGTGGATCTTGATAATCAGCATGATTTTCTGTTTAAGATGCCAAATGGGCATAACCAGAGGCTGCAGCAGGAACTGTTAGAGAAGGCAAATGCTGAAATTTTTTCTCTTCCTACTAATTATGCTCATCTAGCAAAGAAAAATGGAATCATAACAAACTGTACTACATCCACTGGGCAAGCTAATTTTTCAAGAAGAACTTCTTCTCCCAGTCTTTACTCAAACGGGAGTTCTTCAGGCTTTGGAGCTTTGGAGGGATCTAATGTTCCTTACCAAGAAGCAAATACGCCAGGTTTGGACTTTGATGGCCACGTTGCTGGGGCCTATCCTGTAAATCCGAAGTTGAATATGACAATTAATAATCATCTTGATGCAGTAGCTAGAGGTGATGAACAAGGTCTGAACAGACTGGGAAACAAAGTAGGGCCTGGTCTCCATTCTCCTGTTATGGACCCATCTTATATTCAGTTTTTGCGAAGATCTGATCATGCAACATGTAATGTAACTAGTCCAAGTGGTTATCCTCTGAGTAAAAACCACTTTGGTACTTTGCATGGAGACTTAGAGGGGATTCAGAAGGCATATCTTGAGGCATTGCTTGTTCAAAGGAAGCAACAGTATGAGTTGTCACTTTTAGGTAGATCTGGTGGTTTTAATCATGGTCATTATGGGAACCCACCATATGGGCTTGGTATGTATCCTGGAAATCCAATGGAAAATTATCTGCATCCTTCTATTGGGTCTGGTAGTCCAATGTTTGAGAGTGAGAAGATTGCACGCTTTAATTCTATGTTGAGAAGTTCAATGGGAGGGTCGGTTAGCTCCTGGAACTCAGACATTGGAAAGGACCTAGAACGACGACATGCATCATCGTTATTAGACGAATTCAAGAACAACAAGAACAAGTCTTTTGAACTTGCAGACATTGTTGATCATGTAGTGGAATTCAGTACCGACCAGTATGGAAGTCGCTTTATTCAGCAGAAACTAGAAACTGCCACTGTGGAAGAAAAATTGAAGATATTTCCAGAGATTATTCCTCATGCTCGCACCTTGATGACTGATGTATTCGGAAATTATGTCATACAGAAATTTTTTGAGCATGGTACAGAAAGCCAAAAAAAAGAATTAACCAGCCAACTTACTGGCCATATTTTGCCTCTCAGTCTTCAAATGTATGGTTGTAGAGTGATCCAGAAGGCTTTGGAAGCAGTTGATGTGAATCAGCAGGCACAGATGGTGTCGGAGCTTAATGGTTCAATCATGAAATGTGTTCGTGATCAGAATGGGAATCATGTCATTCAGAAGTGTATAGAATGCATCCCTCAAGATAAAATCCAATTTATACTTTCATCCTTCTATGGGCAAGTTGTCGCACTATCCACTCATCCTTATGGTTGCCGGGTTATACAGAGGGTTCTGGAACGTTGCAATGATTCAAACATGCAACAAATAATCATGGATGAAATCATGCAATCTGTATGCGTTTTGGCACAAGATCAATATGGAAATTACGTCATTCAGCACATCCTTGAACATGGTAAACCGCACGAACGGACTGCAATCATCCACAAGCTTGCCGGACAAATTGTGAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAGAAGTGCTTAACTTTTGGGAATGCTGAGGAGCGTCAGTTTTTAGTGAATGAGATGCTGGGTTCAACTGATGCAAATGAGCCCTTACAGGCCATGATGAAGGATCCGTTTGGAAACTACGTTGTGCAAAAGGTTCTTGAGACGTGTGATGATCAGAGTCTTGAGTTGATTCTTTCTCGCATTAAGGTTCATTTGACTGCCCTGAAGAAGTATACGTATGGCAAACATATCGTTTCTCGTGTCGAGAAACTCATCACAACTGGAGAAAGGCGCATAGGGTTGTCAGCCTCGTTTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1016

Amino Acids

112.39

Weight (kDa)

6.44

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 307 - 497 6.1e-44 Nucleic acid binding protein NABP
NABP PF07990 499 - 679 1.1e-67 Nucleic acid binding protein NABP
PUF PF00806 684 - 718 3.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 722 - 751 4.1e-08 Pumilio-family RNA binding repeat
PUF PF00806 756 - 789 5.6e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 794 - 865 3.5e-06 NOP9-like PUF repeat domain
PUF PF00806 828 - 863 2.5e-12 Pumilio-family RNA binding repeat
PUF PF00806 866 - 898 8.3e-10 Pumilio-family RNA binding repeat
PUF PF00806 901 - 935 2.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 947 - 976 6.2e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1799
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 2957
AciI CCGC 5 cut(s) 144, 155, 384, 387, 2663
AclI AACGTT 1 cut(s) 2542
AclWI GGATC 7 cut(s) 178, 734, 1131, 1381, 2302, 2843, 2856
AcoI YGGCCR 2 cut(s) 1423, 2263
AcsI RAATTY 5 cut(s) 43, 1215, 2000, 2060, 2207
AcuI CTGAAG 2 cut(s) 1335, 2969
AfaI GTAC 4 cut(s) 1279, 1663, 2068, 2224
AfiI CCNNNNNNNGG 8 cut(s) 114, 599, 684, 947, 948, 1799, 1853, 2478
AflII CTTAAG 1 cut(s) 1060
AflIII ACRYGT 2 cut(s) 1610, 2888
AhlI ACTAGT 2 cut(s) 224, 1622
AjiI CACGTC 1 cut(s) 2889
AjnI CCWGG 5 cut(s) 813, 1405, 1543, 1816, 1940
AloI GAACNNNNNNTCC 4 cut(s) 1306, 1338, 1365, 1397
Alw26I GTCTC 6 cut(s) 9, 197, 405, 1553, 1668, 2879
AlwI GGATC 7 cut(s) 178, 734, 1131, 1381, 2302, 2843, 2856
AlwNI CAGNNNCTG 2 cut(s) 1180, 1192
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 8 cut(s) 686, 811, 934, 1423, 1437, 1541, 2263, 2835
ApeKI GCWGC 4 cut(s) 91, 1111, 1180, 1183
ApoI RAATTY 5 cut(s) 43, 1215, 2000, 2060, 2207
AseI ATTAAT 1 cut(s) 1473
Asp700I GAANNNNTTC 5 cut(s) 78, 428, 802, 1315, 1318
AspLEI GCGC 2 cut(s) 96, 3018
AspS9I GGNCC 7 cut(s) 32, 674, 872, 1437, 1541, 1567, 1963
AsuC2I CCSGG 1 cut(s) 2519
AsuHPI GGTGA 2 cut(s) 722, 1514
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 5 cut(s) 32, 674, 872, 1567, 1963
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 2 cut(s) 1425, 2265
BamHI GGATCC 1 cut(s) 2848
BanII GRGCYC 1 cut(s) 2829
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
BauI CACGAG 1 cut(s) 2982
BbsI GAAGAC 2 cut(s) 384, 2276
BbvCI CCTCAGC 1 cut(s) 2772
BbvI GCAGC 4 cut(s) 103, 1123, 1167, 1195
BccI CCATC 7 cut(s) 496, 569, 697, 753, 1415, 1579, 2347
BcgI CGANNNNNNTGC 4 cut(s) 2657, 2691, 2956, 2990
BciT130I CCWGG 5 cut(s) 815, 1407, 1545, 1818, 1942
BciVI GTATCC 1 cut(s) 1824
BclI TGATCA 5 cut(s) 652, 1600, 2047, 2395, 2896
BcnI CCSGG 1 cut(s) 2519
BcoDI GTCTC 6 cut(s) 9, 197, 405, 1553, 1668, 2879
BcuI ACTAGT 2 cut(s) 224, 1622
BfaI CTAG 7 cut(s) 225, 987, 1247, 1497, 1623, 1967, 2105
BfmI CTRYAG 4 cut(s) 777, 945, 1083, 1181
BfrI CTTAAG 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 1824
BglI GCCNNNNNGGC 1 cut(s) 1165
BglII AGATCT 2 cut(s) 1595, 1757
BisI GCNGC 5 cut(s) 92, 385, 1112, 1181, 1184
BlsI GCNGC 5 cut(s) 93, 386, 1113, 1182, 1185
Bme1390I CCNGG 6 cut(s) 815, 1407, 1545, 1818, 1942, 2519
Bme18I GGWCC 5 cut(s) 32, 674, 872, 1567, 1963
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 2889
BmgT120I GGNCC 7 cut(s) 32, 674, 872, 1437, 1541, 1567, 1963
BmiI GGNNCC 6 cut(s) 34, 676, 1438, 1569, 1790, 2850
BmrFI CCNGG 6 cut(s) 815, 1407, 1545, 1818, 1942, 2519
BmrI ACTGGG 4 cut(s) 609, 1299, 1320, 1535
BmsI GCATC 9 cut(s) 43, 869, 1146, 1477, 1852, 1991, 2445, 2790, 2806
BmuI ACTGGG 4 cut(s) 609, 1299, 1320, 1535
BpiI GAAGAC 2 cut(s) 384, 2276
BpmI CTGGAG 2 cut(s) 436, 3027
Bpu10I CCTNAGC 1 cut(s) 2772
BpuEI CTTGAG 4 cut(s) 1724, 2427, 2903, 2927
BpuMI CCSGG 1 cut(s) 2519
BsaAI YACGTR 1 cut(s) 2961
BsaBI GATNNNNATC 2 cut(s) 744, 2337
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 1 cut(s) 832
Bsc4I CCNNNNNNNGG 8 cut(s) 114, 599, 684, 947, 948, 1799, 1853, 2478
Bse1I ACTGG 9 cut(s) 419, 604, 749, 1294, 1326, 1530, 2074, 2266, 3010
Bse3DI GCAATG 2 cut(s) 1709, 2554
Bse8I GATNNNNATC 2 cut(s) 744, 2337
BseBI CCWGG 5 cut(s) 815, 1407, 1545, 1818, 1942
BseDI CCNNGG 1 cut(s) 832
BseJI GATNNNNATC 2 cut(s) 744, 2337
BseLI CCNNNNNNNGG 8 cut(s) 114, 599, 684, 947, 948, 1799, 1853, 2478
BseMI GCAATG 2 cut(s) 1709, 2554
BseMII CTCAG 8 cut(s) 74, 219, 500, 543, 1634, 1962, 2293, 2763
BseNI ACTGG 9 cut(s) 419, 604, 749, 1294, 1326, 1530, 2074, 2266, 3010
BseRI GAGGAG 2 cut(s) 433, 2789
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 4 cut(s) 103, 1123, 1167, 1195
BseYI CCCAGC 2 cut(s) 1433, 2803
BshFI GGCC 8 cut(s) 688, 813, 936, 1425, 1439, 1543, 2265, 2837
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 2 cut(s) 2518, 2697
BslFI GGGAC 2 cut(s) 137, 660
BslI CCNNNNNNNGG 8 cut(s) 114, 599, 684, 947, 948, 1799, 1853, 2478
BsmAI GTCTC 6 cut(s) 9, 197, 405, 1553, 1668, 2879
BsmBI CGTCTC 1 cut(s) 2879
BsmFI GGGAC 2 cut(s) 137, 660
BsmI GAATGC 3 cut(s) 520, 2438, 2773
BsnI GGCC 8 cut(s) 688, 813, 936, 1425, 1439, 1543, 2265, 2837
Bso31I GGTCTC 1 cut(s) 1553
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 154, 2829
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 5 cut(s) 144, 155, 384, 387, 2663
BspANI GGCC 8 cut(s) 688, 813, 936, 1425, 1439, 1543, 2265, 2837
BspCNI CTCAG 8 cut(s) 75, 218, 501, 544, 1635, 1961, 2292, 2764
BspHI TCATGA 1 cut(s) 2379
BspLI GGNNCC 6 cut(s) 34, 676, 1438, 1569, 1790, 2850
BspMAI CTGCAG 2 cut(s) 781, 1185
BspPI GGATC 7 cut(s) 178, 734, 1131, 1381, 2302, 2843, 2856
BspQI GCTCTTC 1 cut(s) 793
BspTI CTTAAG 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 1553
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 1709, 2554
BsrI ACTGG 9 cut(s) 419, 604, 749, 1294, 1326, 1530, 2074, 2266, 3010
BssECI CCNNGG 1 cut(s) 832
BssNAI GTATAC 1 cut(s) 2958
BssSI CACGAG 1 cut(s) 2982
BssT1I CCWWGG 1 cut(s) 832
Bst1107I GTATAC 1 cut(s) 2958
Bst2BI CACGAG 1 cut(s) 2982
Bst2UI CCWGG 5 cut(s) 815, 1407, 1545, 1818, 1942
Bst4CI ACNGT 7 cut(s) 163, 339, 929, 1194, 1277, 1734, 2119
Bst6I CTCTTC 2 cut(s) 793, 1230
BstAFI CTTAAG 1 cut(s) 1060
BstBAI YACGTR 1 cut(s) 2961
BstC8I GCNNGC 6 cut(s) 105, 1296, 1894, 2164, 2346, 2694
BstDEI CTNAG 9 cut(s) 83, 205, 509, 552, 1643, 1678, 1948, 2279, 2772
BstDSI CCRYGG 1 cut(s) 832
BstHHI GCGC 2 cut(s) 96, 3018
BstMAI GTCTC 6 cut(s) 9, 197, 405, 1553, 1668, 2879
BstMWI GCNNNNNNNGC 5 cut(s) 100, 475, 1165, 1247, 2324
BstNI CCWGG 5 cut(s) 815, 1407, 1545, 1818, 1942
BstNSI RCATGY 4 cut(s) 107, 1614, 1982, 2563
BstSCI CCNGG 6 cut(s) 813, 1405, 1543, 1816, 1940, 2517
BstSFI CTRYAG 4 cut(s) 777, 945, 1083, 1181
BstSLI GKGCMC 1 cut(s) 154
BstSNI TACGTA 1 cut(s) 2961
BstV1I GCAGC 4 cut(s) 103, 1123, 1167, 1195
BstV2I GAAGAC 2 cut(s) 384, 2276
BstX2I RGATCY 5 cut(s) 1123, 1373, 1595, 1757, 2848
BstXI CCANNNNNNTGG 5 cut(s) 472, 733, 752, 1433, 2261
BstYI RGATCY 5 cut(s) 1123, 1373, 1595, 1757, 2848
BstZ17I GTATAC 1 cut(s) 2958
BsuI GTATCC 1 cut(s) 1824
BsuRI GGCC 8 cut(s) 688, 813, 936, 1425, 1439, 1543, 2265, 2837
BtgI CCRYGG 1 cut(s) 832
BtrI CACGTC 1 cut(s) 2889
BtsIMutI CAGTG 2 cut(s) 1287, 2115
Cac8I GCNNGC 6 cut(s) 105, 1296, 1894, 2164, 2346, 2694
CaiI CAGNNNCTG 2 cut(s) 1180, 1192
CciI TCATGA 1 cut(s) 2379
CfoI GCGC 2 cut(s) 96, 3018
Cfr13I GGNCC 7 cut(s) 32, 674, 872, 1437, 1541, 1567, 1963
CseI GACGC 1 cut(s) 2768
Csp6I GTAC 4 cut(s) 1278, 1662, 2067, 2223
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviQI GTAC 4 cut(s) 1278, 1662, 2067, 2223
DdeI CTNAG 9 cut(s) 83, 205, 509, 552, 1643, 1678, 1948, 2279, 2772
EaeI YGGCCR 2 cut(s) 1423, 2263
Eam1104I CTCTTC 2 cut(s) 793, 1230
EarI CTCTTC 2 cut(s) 793, 1230
Eco105I TACGTA 1 cut(s) 2961
Eco130I CCWWGG 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 2829
Eco31I GGTCTC 1 cut(s) 1553
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 5 cut(s) 32, 674, 872, 1567, 1963
Eco57I CTGAAG 2 cut(s) 1335, 2969
Eco88I CYCGRG 1 cut(s) 12
EcoO109I RGGNCCY 5 cut(s) 32, 674, 1437, 1541, 1963
EcoRI GAATTC 2 cut(s) 2000, 2060
EcoRII CCWGG 5 cut(s) 813, 1405, 1543, 1816, 1940
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 832
EcoT22I ATGCAT 3 cut(s) 792, 1984, 2438
EcoT38I GRGCYC 1 cut(s) 2829
ErhI CCWWGG 1 cut(s) 832
Esp3I CGTCTC 1 cut(s) 2879
FalI AAGNNNNNCTT 4 cut(s) 1300, 1332, 1303, 1335
FaqI GGGAC 2 cut(s) 137, 660
FauI CCCGC 2 cut(s) 162, 380
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 5 cut(s) 652, 1600, 2047, 2395, 2896
FblI GTMKAC 1 cut(s) 2957
Fnu4HI GCNGC 5 cut(s) 92, 385, 1112, 1181, 1184
FriOI GRGCYC 1 cut(s) 2829
Fsp4HI GCNGC 5 cut(s) 92, 385, 1112, 1181, 1184
FspBI CTAG 7 cut(s) 225, 987, 1247, 1497, 1623, 1967, 2105
GlaI GCGC 2 cut(s) 95, 3017
GluI GCNGC 5 cut(s) 92, 385, 1112, 1181, 1184
GsaI CCCAGC 2 cut(s) 1437, 2807
GsuI CTGGAG 2 cut(s) 436, 3027
HaeIII GGCC 8 cut(s) 688, 813, 936, 1425, 1439, 1543, 2265, 2837
HapII CCGG 2 cut(s) 2518, 2697
HgaI GACGC 1 cut(s) 2768
HhaI GCGC 2 cut(s) 96, 3018
Hin6I GCGC 2 cut(s) 94, 3016
HinP1I GCGC 2 cut(s) 94, 3016
HindIII AAGCTT 1 cut(s) 2690
HpaII CCGG 2 cut(s) 2518, 2697
HphI GGTGA 2 cut(s) 722, 1514
Hpy166II GTNNAC 2 cut(s) 2660, 2958
Hpy8I GTNNAC 2 cut(s) 2660, 2958
Hpy99I CGWCG 1 cut(s) 1977
HpyAV CCTTC 9 cut(s) 606, 638, 885, 1195, 1687, 1857, 2308, 2482, 2839
HpyCH4III ACNGT 7 cut(s) 163, 339, 929, 1194, 1277, 1734, 2119
HpyCH4IV ACGT 7 cut(s) 1099, 1428, 2542, 2631, 2865, 2888, 2960
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 475, 1165, 1247, 2324
HpyF3I CTNAG 9 cut(s) 83, 205, 509, 552, 1643, 1678, 1948, 2279, 2772
HpySE526I ACGT 7 cut(s) 1099, 1428, 2542, 2631, 2865, 2888, 2960
HspAI GCGC 2 cut(s) 94, 3016
Ksp22I TGATCA 5 cut(s) 652, 1600, 2047, 2395, 2896
LguI GCTCTTC 1 cut(s) 793
LmnI GCTCC 6 cut(s) 141, 525, 1361, 1943, 2362, 2776
Lsp1109I GCAGC 4 cut(s) 103, 1123, 1167, 1195
LweI GCATC 9 cut(s) 43, 869, 1146, 1477, 1852, 1991, 2445, 2790, 2806
MaeI CTAG 7 cut(s) 225, 987, 1247, 1497, 1623, 1967, 2105
MaeII ACGT 7 cut(s) 1099, 1428, 2542, 2631, 2865, 2888, 2960
MaeIII GTNAC 4 cut(s) 220, 698, 1618, 1743
MbiI CCGCTC 1 cut(s) 144
MflI RGATCY 5 cut(s) 1123, 1373, 1595, 1757, 2848
MhlI GDGCHC 2 cut(s) 154, 2829
MlsI TGGCCA 2 cut(s) 1425, 2265
MluNI TGGCCA 2 cut(s) 1425, 2265
MlyI GAGTC 3 cut(s) 782, 2725, 2911
MmeI TCCRAC 1 cut(s) 2340
Mox20I TGGCCA 2 cut(s) 1425, 2265
Mph1103I ATGCAT 3 cut(s) 792, 1984, 2438
MroXI GAANNNNTTC 5 cut(s) 78, 428, 802, 1315, 1318
MscI TGGCCA 2 cut(s) 1425, 2265
MslI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1485, 2171, 2508
Msp20I TGGCCA 2 cut(s) 1425, 2265
MspCI CTTAAG 1 cut(s) 1060
MspI CCGG 2 cut(s) 2518, 2697
MspR9I CCNGG 6 cut(s) 815, 1407, 1545, 1818, 1942, 2519
Mva1269I GAATGC 3 cut(s) 520, 2438, 2773
MvaI CCWGG 5 cut(s) 815, 1407, 1545, 1818, 1942
MwoI GCNNNNNNNGC 5 cut(s) 100, 475, 1165, 1247, 2324
NciI CCSGG 1 cut(s) 2519
NcoI CCATGG 1 cut(s) 832
NdeI CATATG 1 cut(s) 1798
NlaIV GGNNCC 6 cut(s) 34, 676, 1438, 1569, 1790, 2850
NmuCI GTSAC 2 cut(s) 220, 1743
NsiI ATGCAT 3 cut(s) 792, 1984, 2438
NspI RCATGY 4 cut(s) 107, 1614, 1982, 2563
PaeI GCATGC 1 cut(s) 107
PagI TCATGA 1 cut(s) 2379
PciI ACATGT 1 cut(s) 1610
PciSI GCTCTTC 1 cut(s) 793
PctI GAATGC 3 cut(s) 520, 2438, 2773
PdmI GAANNNNTTC 5 cut(s) 78, 428, 802, 1315, 1318
PfeI GAWTC 9 cut(s) 79, 268, 722, 1263, 1687, 2338, 2407, 2552, 2914
PflMI CCANNNNNTGG 1 cut(s) 1799
PfoI TCCNGGA 2 cut(s) 1816, 1940
PkrI GCNGC 5 cut(s) 93, 386, 1113, 1182, 1185
PleI GAGTC 3 cut(s) 781, 2724, 2910
PpsI GAGTC 3 cut(s) 781, 2724, 2910
Ppu21I YACGTR 1 cut(s) 2961
PpuMI RGGWCCY 3 cut(s) 32, 674, 1963
PscI ACATGT 1 cut(s) 1610
PshBI ATTAAT 1 cut(s) 1473
Psp1406I AACGTT 1 cut(s) 2542
Psp5II RGGWCCY 3 cut(s) 32, 674, 1963
Psp6I CCWGG 5 cut(s) 813, 1405, 1543, 1816, 1940
PspFI CCCAGC 2 cut(s) 1433, 2803
PspGI CCWGG 5 cut(s) 813, 1405, 1543, 1816, 1940
PspN4I GGNNCC 6 cut(s) 34, 676, 1438, 1569, 1790, 2850
PspPI GGNCC 7 cut(s) 32, 674, 872, 1437, 1541, 1567, 1963
PspPPI RGGWCCY 3 cut(s) 32, 674, 1963
PsrI GAACNNNNNNTAC 2 cut(s) 894, 926
PstI CTGCAG 2 cut(s) 781, 1185
PstNI CAGNNNCTG 2 cut(s) 1180, 1192
PsuI RGATCY 5 cut(s) 1123, 1373, 1595, 1757, 2848
RsaI GTAC 4 cut(s) 1279, 1663, 2068, 2224
RsaNI GTAC 4 cut(s) 1278, 1662, 2067, 2223
RseI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1485, 2171, 2508
SapI GCTCTTC 1 cut(s) 793
SatI GCNGC 5 cut(s) 92, 385, 1112, 1181, 1184
Sau96I GGNCC 7 cut(s) 32, 674, 872, 1437, 1541, 1567, 1963
SchI GAGTC 3 cut(s) 782, 2725, 2911
ScrFI CCNGG 6 cut(s) 815, 1407, 1545, 1818, 1942, 2519
SduI GDGCHC 2 cut(s) 154, 2829
SfaNI GCATC 9 cut(s) 43, 869, 1146, 1477, 1852, 1991, 2445, 2790, 2806
SfcI CTRYAG 4 cut(s) 777, 945, 1083, 1181
SinI GGWCC 5 cut(s) 32, 674, 872, 1567, 1963
SmiMI CAYNNNNRTG 6 cut(s) 131, 553, 731, 1485, 2171, 2508
SmlI CTYRAG 5 cut(s) 1060, 1703, 2442, 2882, 2906
SmoI CTYRAG 5 cut(s) 1060, 1703, 2442, 2882, 2906
SnaBI TACGTA 1 cut(s) 2961
SpeI ACTAGT 2 cut(s) 224, 1622
SphI GCATGC 1 cut(s) 107
SsiI CCGC 5 cut(s) 144, 155, 384, 387, 2663
SspMI CTAG 7 cut(s) 225, 987, 1247, 1497, 1623, 1967, 2105
StyD4I CCNGG 6 cut(s) 813, 1405, 1543, 1816, 1940, 2517
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 7 cut(s) 163, 339, 929, 1194, 1277, 1734, 2119
TaiI ACGT 7 cut(s) 1102, 1431, 2545, 2634, 2868, 2891, 2963
TaqI TCGA 2 cut(s) 271, 2988
TaqII GACCGA 1 cut(s) 860
TatI WGTACW 1 cut(s) 1277
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 9 cut(s) 79, 268, 722, 1263, 1687, 2338, 2407, 2552, 2914
TscAI CASTG 2 cut(s) 1294, 2122
TseFI GTSAC 2 cut(s) 220, 1743
TseI GCWGC 4 cut(s) 91, 1111, 1180, 1183
Tsp45I GTSAC 2 cut(s) 220, 1743
TspGWI ACGGA 2 cut(s) 2686, 2841
TspRI CASTG 2 cut(s) 1294, 2122
Van91I CCANNNNNTGG 1 cut(s) 1799
Vha464I CTTAAG 1 cut(s) 1060
VpaK11BI GGWCC 5 cut(s) 32, 674, 872, 1567, 1963
VspI ATTAAT 1 cut(s) 1473
XapI RAATTY 5 cut(s) 43, 1215, 2000, 2060, 2207
XceI RCATGY 4 cut(s) 107, 1614, 1982, 2563
XmiI GTMKAC 1 cut(s) 2957
XmnI GAANNNNTTC 5 cut(s) 78, 428, 802, 1315, 1318
XspI CTAG 7 cut(s) 225, 987, 1247, 1497, 1623, 1967, 2105
Zsp2I ATGCAT 3 cut(s) 792, 1984, 2438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.