pycom07g16530

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007939
Physical Location & Seq
Forward (+)
18969299 .. 18971037
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16530.2

Sequence Viewer

Length: 1029 bp
ATGCTATCAACCCGAGACAGTAATCTGCAAGGACCCAATGGGAATTTAGAAGATGCTTTACAGAGTGAGCTTGAATTGATTCTGAGAGAGCAGCGCAATCAGCATGCCATTCATCGGGAGAGGGACATGAATATGTATAGGAGCGGCAGTGCCCCGCCAACAGTTGAGGGATCGCTAACTGCTGTCGGTAGTCTCTTTAGGAACTCAGACTTTAGGGATGTGACTAGTAGGATTAGTAATAACAATGGGGTTTTGTCCGAAGATGAGATTCGATCTCATCCTGCATATCTTTCATACTACTATTCACATGATAACATTAACCCAAGATTGCCTCCACCGTTGTTGTCAAGAGAGGATTGGCGTGTTGCACAAAGGTTTCAGGGTGGCGGGTCTTCTTTTGAGGGCATTGGAGACTGGAGGAGGAAGAAGTTGGCTGATGATCGTGATAGTTTGTCGCTGTTTGCCACGCAGTTGGGGCTACAAAAGGCAGAGAACGATATGATGGAACTGAGGAATGCTAATGGGAGCAATCTCCCTAAACAGACTTCATCTGAGTGGCTTGATAGAAGCTCAGATGGTTTGATTGGTTTGTCGACAACTGGGCTTGGTGGAAGGAGAAAGAGTTTTGCTGACATTTTTCAGGAAGGACTTGATCAAACTGCCTCCTTGTCAGGTCCCCTGTCAAGGCCATCAAGTCGTAACGCATTTGGTGATATCATGGATTCCACAGGCATGGCTGATCCCCATCCTGTTGCGTTATCTAATGGAGTGGAGTCTGCAGAAGGTATGCATAATGGAAGAGCTTCACGTGGCCTGGTGGGAGTTCAGAGCCATGGCTCAACAACTTCTAATTCCTTTGCATCTGCTGTCGGGCCTTCTTTGCCAAGAAGCAGAACCCCTGAACAACAACTACTTGGGAGGTCTAACAGTTTTGGCCTCCCCCCTGTAGGGAGTAGAGTTTTCCCAACCGAGAGGAAGAATGTTGCTAGTCTAGATATTCAAAATGATCGAATCTTCCAGCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

343

Amino Acids

37.47

Weight (kDa)

6.1

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 593
AciI CCGC 3 cut(s) 144, 155, 387
AclWI GGATC 2 cut(s) 178, 734
AcsI RAATTY 1 cut(s) 43
AcvI CACGTG 1 cut(s) 809
AfiI CCNNNNNNNGG 4 cut(s) 114, 684, 947, 948
AgsI TTSAA 2 cut(s) 74, 1001
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 813
AluBI AGCT 3 cut(s) 70, 570, 803
AluI AGCT 3 cut(s) 70, 570, 803
Alw26I GTCTC 3 cut(s) 9, 197, 405
AlwI GGATC 2 cut(s) 178, 734
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 4 cut(s) 686, 811, 872, 934
ApeKI GCWGC 1 cut(s) 91
ApoI RAATTY 1 cut(s) 43
Asp700I GAANNNNTTC 2 cut(s) 78, 802
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 3 cut(s) 32, 674, 872
AsuHPI GGTGA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 2 cut(s) 32, 674
BaeGI GKGCMC 1 cut(s) 154
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
BbrPI CACGTG 1 cut(s) 809
BbsI GAAGAC 1 cut(s) 384
BbvI GCAGC 1 cut(s) 103
BccI CCATC 4 cut(s) 496, 569, 697, 753
BciT130I CCWGG 1 cut(s) 815
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 9, 197, 405
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 3 cut(s) 225, 987, 992
BfmI CTRYAG 2 cut(s) 777, 945
BisI GCNGC 2 cut(s) 92, 145
BlsI GCNGC 2 cut(s) 93, 146
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 2 cut(s) 32, 674
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 3 cut(s) 32, 674, 872
BmiI GGNNCC 2 cut(s) 34, 676
BmrFI CCNGG 1 cut(s) 815
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 43, 869
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 744
BsaJI CCNNGG 1 cut(s) 832
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 607, 637
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 684, 947, 948
Bse1I ACTGG 2 cut(s) 419, 604
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 1 cut(s) 815
BseDI CCNNGG 1 cut(s) 832
BseGI GGATG 3 cut(s) 223, 277, 745
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 4 cut(s) 114, 684, 947, 948
BseMII CTCAG 5 cut(s) 74, 219, 500, 543, 585
BseNI ACTGG 2 cut(s) 419, 604
BseRI GAGGAG 1 cut(s) 433
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 1 cut(s) 103
BshFI GGCC 4 cut(s) 688, 813, 874, 936
BsiHKCI CYCGRG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 137, 660
BslI CCNNNNNNNGG 4 cut(s) 114, 684, 947, 948
BsmAI GTCTC 3 cut(s) 9, 197, 405
BsmFI GGGAC 2 cut(s) 137, 660
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 4 cut(s) 688, 813, 874, 936
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 154
Bsp143I GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 3 cut(s) 144, 155, 387
BspANI GGCC 4 cut(s) 688, 813, 874, 936
BspCNI CTCAG 5 cut(s) 75, 218, 501, 544, 584
BspLI GGNNCC 2 cut(s) 34, 676
BspMAI CTGCAG 1 cut(s) 781
BspPI GGATC 2 cut(s) 178, 734
BspQI GCTCTTC 1 cut(s) 793
BsrBI CCGCTC 1 cut(s) 144
BsrI ACTGG 2 cut(s) 419, 604
BssECI CCNNGG 1 cut(s) 832
BssMI GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
BssT1I CCWWGG 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 4 cut(s) 20, 163, 339, 929
Bst6I CTCTTC 1 cut(s) 793
BstBAI YACGTR 1 cut(s) 809
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 5 cut(s) 83, 205, 509, 552, 571
BstDSI CCRYGG 1 cut(s) 832
BstF5I GGATG 3 cut(s) 223, 277, 745
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 6 cut(s) 173, 275, 442, 655, 742, 1009
BstMAI GTCTC 3 cut(s) 9, 197, 405
BstMBI GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
BstMWI GCNNNNNNNGC 3 cut(s) 100, 475, 880
BstNI CCWGG 1 cut(s) 815
BstNSI RCATGY 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 813
BstSFI CTRYAG 2 cut(s) 777, 945
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 103
BstV2I GAAGAC 1 cut(s) 384
BstXI CCANNNNNNTGG 2 cut(s) 472, 733
BsuRI GGCC 4 cut(s) 688, 813, 874, 936
BtgI CCRYGG 1 cut(s) 832
BtsCI GGATG 3 cut(s) 223, 277, 745
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 105
CfoI GCGC 1 cut(s) 96
Cfr13I GGNCC 3 cut(s) 32, 674, 872
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviAII CATG 7 cut(s) 104, 127, 308, 718, 733, 833, 1022
DdeI CTNAG 5 cut(s) 83, 205, 509, 552, 571
DpnI GATC 6 cut(s) 172, 274, 441, 654, 741, 1008
DpnII GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 832
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 2 cut(s) 32, 674
Eco72I CACGTG 1 cut(s) 809
Eco88I CYCGRG 1 cut(s) 12
EcoO109I RGGNCCY 2 cut(s) 32, 674
EcoRII CCWGG 1 cut(s) 813
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 832
EcoT22I ATGCAT 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 832
FaeI CATG 7 cut(s) 107, 130, 311, 721, 736, 836, 1025
FaqI GGGAC 2 cut(s) 137, 660
FatI CATG 7 cut(s) 103, 126, 307, 717, 732, 832, 1021
FauI CCCGC 2 cut(s) 162, 380
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 2 cut(s) 92, 145
FokI GGATG 3 cut(s) 230, 264, 732
Fsp4HI GCNGC 2 cut(s) 92, 145
FspBI CTAG 3 cut(s) 225, 987, 992
GlaI GCGC 1 cut(s) 95
GluI GCNGC 2 cut(s) 92, 145
GsuI CTGGAG 1 cut(s) 436
HaeIII GGCC 4 cut(s) 688, 813, 874, 936
HhaI GCGC 1 cut(s) 96
Hin1II CATG 7 cut(s) 107, 130, 311, 721, 736, 836, 1025
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HinfI GANTC 5 cut(s) 79, 268, 722, 773, 1011
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 1 cut(s) 594
Hpy188I TCNGA 6 cut(s) 84, 208, 259, 553, 574, 828
Hpy188III TCNNGA 5 cut(s) 116, 348, 443, 641, 992
Hpy8I GTNNAC 1 cut(s) 594
HpyAV CCTTC 4 cut(s) 606, 638, 776, 885
HpyCH4III ACNGT 4 cut(s) 20, 163, 339, 929
HpyCH4IV ACGT 1 cut(s) 808
HpyCH4V TGCA 6 cut(s) 28, 284, 368, 779, 790, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 475, 880
HpyF3I CTNAG 5 cut(s) 83, 205, 509, 552, 571
HpySE526I ACGT 1 cut(s) 808
Hsp92II CATG 7 cut(s) 107, 130, 311, 721, 736, 836, 1025
HspAI GCGC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
LguI GCTCTTC 1 cut(s) 793
LmnI GCTCC 2 cut(s) 141, 525
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 2 cut(s) 43, 869
MaeI CTAG 3 cut(s) 225, 987, 992
MaeII ACGT 1 cut(s) 808
MaeIII GTNAC 2 cut(s) 220, 698
MalI GATC 6 cut(s) 172, 274, 441, 654, 741, 1008
MbiI CCGCTC 1 cut(s) 144
MboI GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
MboII GAAGA 7 cut(s) 62, 272, 384, 436, 810, 988, 1006
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 3 cut(s) 43, 74, 850
MlyI GAGTC 1 cut(s) 782
Mph1103I ATGCAT 1 cut(s) 792
MroXI GAANNNNTTC 2 cut(s) 78, 802
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 3 cut(s) 131, 553, 731
MspR9I CCNGG 1 cut(s) 815
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 815
MwoI GCNNNNNNNGC 3 cut(s) 100, 475, 880
NcoI CCATGG 1 cut(s) 832
NdeII GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
NlaIII CATG 7 cut(s) 107, 130, 311, 721, 736, 836, 1025
NlaIV GGNNCC 2 cut(s) 34, 676
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 792
NspI RCATGY 1 cut(s) 107
PaeI GCATGC 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 793
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 2 cut(s) 78, 802
PfeI GAWTC 4 cut(s) 79, 268, 722, 1011
PkrI GCNGC 2 cut(s) 93, 146
PleI GAGTC 1 cut(s) 781
PmaCI CACGTG 1 cut(s) 809
PmlI CACGTG 1 cut(s) 809
PpsI GAGTC 1 cut(s) 781
Ppu21I YACGTR 1 cut(s) 809
PpuMI RGGWCCY 2 cut(s) 32, 674
Psp5II RGGWCCY 2 cut(s) 32, 674
Psp6I CCWGG 1 cut(s) 813
PspCI CACGTG 1 cut(s) 809
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 2 cut(s) 34, 676
PspPI GGNCC 3 cut(s) 32, 674, 872
PspPPI RGGWCCY 2 cut(s) 32, 674
PsrI GAACNNNNNNTAC 2 cut(s) 894, 926
PstI CTGCAG 1 cut(s) 781
RseI CAYNNNNRTG 3 cut(s) 131, 553, 731
SalI GTCGAC 1 cut(s) 592
SapI GCTCTTC 1 cut(s) 793
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 92, 145
Sau3AI GATC 6 cut(s) 170, 272, 439, 652, 739, 1006
Sau96I GGNCC 3 cut(s) 32, 674, 872
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 1 cut(s) 815
SduI GDGCHC 1 cut(s) 154
SetI ASST 8 cut(s) 72, 377, 572, 676, 787, 805, 811, 923
SfaNI GCATC 2 cut(s) 43, 869
SfcI CTRYAG 2 cut(s) 777, 945
SinI GGWCC 2 cut(s) 32, 674
SmiMI CAYNNNNRTG 3 cut(s) 131, 553, 731
SpeI ACTAGT 1 cut(s) 224
SphI GCATGC 1 cut(s) 107
Sse9I AATT 3 cut(s) 43, 74, 850
SsiI CCGC 3 cut(s) 144, 155, 387
SspMI CTAG 3 cut(s) 225, 987, 992
StyD4I CCNGG 1 cut(s) 813
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 4 cut(s) 20, 163, 339, 929
TaiI ACGT 1 cut(s) 811
TaqI TCGA 3 cut(s) 271, 593, 1009
TasI AATT 3 cut(s) 43, 74, 850
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 4 cut(s) 79, 268, 722, 1011
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 4 cut(s) 101, 143, 282, 537
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 32, 674
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 991
XceI RCATGY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 78, 802
XspI CTAG 3 cut(s) 225, 987, 992
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.