Rw1G027540

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007939
Physical Location & Seq
Forward (+)
55094403 .. 55100507
6105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027540.1

Sequence Viewer

Length: 2814 bp
ATGGCTAGTGGGGGAAAGATAGATATGCTATCAACGCTAGATAATAATGTGCGAGGTTCAAATGGGAATTTAGAAGATGCTTTGCAGAGTGAGCTTGAGCTGATTCTAAGGGAGCAGCGCAATCAGCACGCCATTCATCGGGATAGGGAAATGAATATGTATAGGAGTGGCAGTGCACCGCCAACTGTGGAAGGATCGCTGAGTGCTGTGGGTAGTCTGTTTAGGAATTCTGATTTTAGGGATAGGGACATGAGAAGTAGGGTTAGTAGTAACAATGGGGGTTTGTCTGAAGATGAGATTCGCTCACATCCTGCTTACCTTTCGTACTATTATTCACATGACAATATCAACCCAAGACTGCCTCCGCCGTTGTTGTCAAGAGAGGATTGGCGTGTTGCGCAGAGGTTTCAGAGTGGGTCTTCTTTTGAAGGTGCTGGAGATTGGAGGAAGAACAATTTAGTTGGCAATGGTGATAAGTCGTTGTTTTCAATGCAGCTGGGGCTTTCTGCACAGAAGGCAGATAATGACTTGATCAAATTTAGAAATGCCAATGGGAGTAGTATGCATAGACAGACGTCATCCGAGTGGCTTGACAGAAATTCGAATAGTTTGACTGGATTCCCTACAAATGGACTTGGTGCAAGGAGGAAGAGTTTTGCTGACATTCTACAGGAAGGAAATGATCATCCAGCTTCCTTGTCAGGCCCTCTGTCAAGGCCATCAAGCCGTAATGCTTTTGGTGATGTCATGAATTCAACTGGCATGGCTGATCCACATCCAGTAGGGTTGTGTAATGGAGTGGATTCCATAGAGGGGTTGCACACCGGAGCAGCTCCACGTGGCTTGATAGGAATGCAGAGCCATGGTACCCCAGTTTCTCATTCATTTGCATCTGCTGGATGTCCTTCTTTGTCAAGAAGCAGAACCCCTGAACCACAATTATTTGGAAGTTCTGGTCTTCCTCCTGTGGGAAGAGCGTTTCCTAATGTTGCTGGTGCAGATATCCAAAATGATAGTTTGCCTGCCATGACTGAGTTGCCCAATATGACAACAAATTTATCTGGCTTAAGCCTCTCAAAAGTTAGATCTGCAGATGAAGATAGTCGTCTTCAGTCTCAGCTTCGTATGGACCTTCATAATCAGTGTGATTTTCTGTTCAACACACCAAACAGTCAGAATCAGAGGCTGCAGCAGCAACTGATAGAGAAGTCAAATGCTGAAAACTTTTCCCTTTCTACAAATTATGCTCATTTGGCAAATAAAAATGGGATTCTAACAAACCGTAATGCATCAAATGGGCATGTTCATTTTCCTAGAAGAACTTCTTCTCCCAGTCTTTACTCAAACGGGAGTTCTTCAGCACTTGGAACTTTGGAAGGGTTCAGTGCTCATTACCAGGATGCAAATACCCCAGGAATCAACTTTGATGGTCATGCTGCCGGGGCATATCCTGTAAATCAGAAGTTGAACAATCATCTCGATGCAGGTTTTACTGGTAGTGGTGACGGACAAAGTATGAACAGATTAGGGAACAAAGTAGGGCCTGGCCTTCATTCTTCGTTTGTGGACCCATCATATATTCAGTTAATGCAAAGAACTGATTATGCAGCACACGCTCTGGCTAGTCCAAGTGGTTATTCTCTGAGTAAAAATAACTTTGGCAATTTGCATGGAGACTTAGAGGGGCTTCAGAAAGCATACCTCGAGGCAATACTTGCTCAAAGGAAGCAACAGTACGAGTTGTCCCTTTTAAGTCAATCTGGGGGGTTTGATCATGGTTATTTTGGGAATCCATCATATGGCCTTGGGATGACATATCCAGGAATCTCAGTGGCAAACTCTGTTCTTCCTTCTGTTGGATCCGGAAGTTCAATGTTTCAGAATGAGAAGATTTCCCTCTTTAATTCTATGATGAGAAGTTCAGTGGGAGGTTCAGTCAGCACCTGGCACTCAGATGCTGGAAATGACTCAGAAGGAAGACATGCGTCATCATTATTAGATGAGTTCAAGAACAACAAGAATAAGTCTTTTGAACTTGCAGATATTGTTGATCACGTAGTTGAATTCAGTACCGATCAGTATGGAAGTCGCTTTATCCAGCAGAAACTCGAAACTGCCACTGTAGAAGAAAAAATGAAAATATTCCCTGAGATTATTCCTCATGCTCGCACCTTGATGACTGATGTTTTTGGAAATTATGTCATACAGAAATTCTTTGAGCATGGTACAGAAAGCCAAAGAAACGAATTGACGAACCAACTTACTGGCCATGTTTTGCCTCTCAGTCTGCAAATGTATGGTTGTAGAGTGATTCAGAAGGCCTTGGAAGTGGTTGACAAGGATCATCAGGCTCAAATGGTGGCAGAACTCGATGGTTCAGTCATGAAATGTGTCCGGGATCAAAATGGGAATCATGTCATTCAGAAGAGTATAGAGTGTATCCCTCAAGATCAAATTCAGTTCATCATTTCATCCTTCTATGGGCAAGTTGTCACACTGTCCACCCATCCTTATGGCTGCCGTGTCATACAGGTTAGTTGTTTGCATTCTTGTTCATTTAGTAGGGAGTATAATTTTATTTCACTATTTACTAGTGTAATCTATTTGGATGCCTATAGAGGGTTCTGGAACATTGTGATGACTCAAAGACGCAAGAAATTATCATGGATGAAGTCATGCAATCTGTATGCATTCTGGCACAGGATCAGTATGGGAATTATGTTATTCAGGTATTCAAAACTATTGGATCTTGGTATGCATGTGGTTGAGCTACAAGGTTTTCTTTTTTTTAGATACATTTGGATTTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

937

Amino Acids

103.53

Weight (kDa)

6.81

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 317 - 680 1.2e-102 Nucleic acid binding protein NABP
PUF PF00806 685 - 719 1.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 723 - 754 1.6e-08 Pumilio-family RNA binding repeat
PUF PF00806 757 - 790 2.3e-09 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 578
AbsI CCTCGAGG 1 cut(s) 1703
Acc16I TGCGCA 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 1475
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 1799
AccIII TCCGGA 1 cut(s) 1862
AciI CCGC 2 cut(s) 179, 365
AclWI GGATC 8 cut(s) 202, 764, 1854, 1867, 2349, 2406, 2711, 2754
AcoI YGGCCR 1 cut(s) 2266
AcsI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
AcuI CTGAAG 4 cut(s) 309, 1094, 1341, 1673
AcvI CACGTG 1 cut(s) 839
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 5 cut(s) 326, 868, 1736, 2071, 2227
AfiI CCNNNNNNNGG 8 cut(s) 138, 629, 813, 968, 1799, 1856, 2481, 2619
AflII CTTAAG 1 cut(s) 1066
AhlI ACTAGT 1 cut(s) 2591
AjnI CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
AjuI GAANNNNNNNTTGG 2 cut(s) 1621, 1653
AloI GAACNNNNNNTCC 2 cut(s) 1312, 1344
AluBI AGCT 7 cut(s) 94, 100, 496, 692, 833, 1120, 2770
AluI AGCT 7 cut(s) 94, 100, 496, 692, 833, 1120, 2770
Alw21I GWGCWC 2 cut(s) 178, 1390
Alw26I GTCTC 2 cut(s) 1119, 1668
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 8 cut(s) 202, 764, 1854, 1867, 2349, 2406, 2711, 2754
AlwNI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
Ama87I CYCGRG 1 cut(s) 1703
Aor13HI TCCGGA 1 cut(s) 1862
AoxI GGCC 7 cut(s) 703, 716, 1541, 1546, 1801, 2266, 2319
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 9 cut(s) 115, 493, 830, 1186, 1189, 1192, 1436, 1607, 2517
ApoI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
Asp700I GAANNNNTTC 3 cut(s) 1321, 1324, 2141
Asp718I GGTACC 1 cut(s) 866
AspLEI GCGC 2 cut(s) 120, 400
AspS9I GGNCC 4 cut(s) 704, 1129, 1541, 1567
AsuC2I CCSGG 2 cut(s) 1441, 2396
AsuHPI GGTGA 3 cut(s) 482, 752, 1514
AsuII TTCGAA 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 1703
AvaII GGWCC 2 cut(s) 1129, 1567
BaeGI GKGCMC 1 cut(s) 178
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 1 cut(s) 2268
BamHI GGATCC 1 cut(s) 1859
BanI GGYRCC 1 cut(s) 866
BarI GAAGNNNNNNTAC 2 cut(s) 1718, 1750
BbrPI CACGTG 1 cut(s) 839
BbsI GAAGAC 4 cut(s) 411, 950, 1100, 1984
Bbv12I GWGCWC 2 cut(s) 178, 1390
BbvI GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BccI CCATC 6 cut(s) 727, 1421, 1579, 1801, 2366, 2514
BceAI ACGGC 3 cut(s) 352, 711, 2505
BciT130I CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BciVI GTATCC 1 cut(s) 2450
BclI TGATCA 4 cut(s) 531, 682, 1771, 2050
BcnI CCSGG 2 cut(s) 1441, 2396
BcoDI GTCTC 2 cut(s) 1119, 1668
BcuI ACTAGT 1 cut(s) 2591
BfaI CTAG 5 cut(s) 6, 38, 1314, 1623, 2592
BfmI CTRYAG 5 cut(s) 668, 1089, 1187, 2121, 2614
BfrI CTTAAG 1 cut(s) 1066
BfuAI ACCTGC 1 cut(s) 1475
BfuI GTATCC 1 cut(s) 2450
BglII AGATCT 1 cut(s) 1085
BisI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
BlsI GCNGC 9 cut(s) 117, 495, 832, 1188, 1191, 1194, 1438, 1609, 2519
Bme1390I CCNGG 7 cut(s) 1397, 1413, 1441, 1545, 1821, 1945, 2396
Bme18I GGWCC 2 cut(s) 1129, 1567
BmeT110I CYCGRG 1 cut(s) 1703
BmgT120I GGNCC 4 cut(s) 704, 1129, 1541, 1567
BmiI GGNNCC 3 cut(s) 868, 1569, 1861
BmrFI CCNGG 7 cut(s) 1397, 1413, 1441, 1545, 1821, 1945, 2396
BmrI ACTGGG 2 cut(s) 866, 1326
BmsI GCATC 7 cut(s) 67, 899, 1298, 1390, 1471, 1945, 2599
BmuI ACTGGG 2 cut(s) 866, 1326
BoxI GACNNNNGTC 2 cut(s) 574, 1984
BpiI GAAGAC 4 cut(s) 411, 950, 1100, 1984
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 2 cut(s) 116, 2430
BpuMI CCSGG 2 cut(s) 1441, 2396
BsaAI YACGTR 2 cut(s) 839, 2056
BsaBI GATNNNNATC 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 575
BsaJI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BsaWI WCCGGW 2 cut(s) 824, 1862
BsaXI ACNNNNNCTCC 8 cut(s) 157, 187, 637, 667, 1312, 1342, 1920, 1950
Bsc4I CCNNNNNNNGG 8 cut(s) 138, 629, 813, 968, 1799, 1856, 2481, 2619
Bse1I ACTGG 7 cut(s) 619, 763, 779, 872, 1332, 1498, 2269
Bse3DI GCAATG 1 cut(s) 472
Bse8I GATNNNNATC 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 1862
BseBI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BseDI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 8 cut(s) 138, 629, 813, 968, 1799, 1856, 2481, 2619
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 9 cut(s) 191, 1023, 1130, 1634, 1842, 1965, 1983, 2139, 2296
BseNI ACTGG 7 cut(s) 619, 763, 779, 872, 1332, 1498, 2269
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BseYI CCCAGC 1 cut(s) 496
BsgI GTGCAG 2 cut(s) 492, 1017
BshFI GGCC 7 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321
BshNI GGYRCC 1 cut(s) 866
BsiHKAI GWGCWC 2 cut(s) 178, 1390
BsiHKCI CYCGRG 1 cut(s) 1703
BsiSI CCGG 4 cut(s) 825, 1440, 1863, 2395
BslFI GGGAC 2 cut(s) 260, 1729
BslI CCNNNNNNNGG 8 cut(s) 138, 629, 813, 968, 1799, 1856, 2481, 2619
BsmAI GTCTC 2 cut(s) 1119, 1668
BsmFI GGGAC 2 cut(s) 260, 1729
BsmI GAATGC 3 cut(s) 858, 2545, 2690
BsnI GGCC 7 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321
BsoBI CYCGRG 1 cut(s) 1703
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 178, 1390
Bsp13I TCCGGA 1 cut(s) 1862
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 2 cut(s) 179, 365
BspANI GGCC 7 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321
BspCNI CTCAG 9 cut(s) 192, 1024, 1129, 1635, 1841, 1964, 1982, 2140, 2295
BspEI TCCGGA 1 cut(s) 1862
BspHI TCATGA 2 cut(s) 747, 2382
BspLI GGNNCC 3 cut(s) 868, 1569, 1861
BspMAI CTGCAG 2 cut(s) 1093, 1191
BspMI ACCTGC 1 cut(s) 1475
BspPI GGATC 8 cut(s) 202, 764, 1854, 1867, 2349, 2406, 2711, 2754
BspQI GCTCTTC 1 cut(s) 967
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 7 cut(s) 619, 763, 779, 872, 1332, 1498, 2269
BssECI CCNNGG 5 cut(s) 862, 1411, 1440, 1804, 2322
BssNI GRCGYC 1 cut(s) 575
BssT1I CCWWGG 3 cut(s) 862, 1804, 2322
Bst2UI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
Bst4CI ACNGT 6 cut(s) 187, 1172, 1283, 1734, 2122, 2499
Bst6I CTCTTC 3 cut(s) 644, 967, 2420
BstACI GRCGYC 1 cut(s) 575
BstAFI CTTAAG 1 cut(s) 1066
BstAPI GCANNNNNTGC 1 cut(s) 1715
BstBAI YACGTR 2 cut(s) 839, 2056
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 129, 1023, 2167
BstDSI CCRYGG 1 cut(s) 862
BstHHI GCGC 2 cut(s) 120, 400
BstMAI GTCTC 2 cut(s) 1119, 1668
BstNI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
BstNSI RCATGY 3 cut(s) 1304, 1985, 2762
BstPAI GACNNNNGTC 2 cut(s) 574, 1984
BstSCI CCNGG 7 cut(s) 1395, 1411, 1439, 1543, 1819, 1943, 2394
BstSFI CTRYAG 5 cut(s) 668, 1089, 1187, 2121, 2614
BstSLI GKGCMC 1 cut(s) 178
BstV1I GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
BstV2I GAAGAC 4 cut(s) 411, 950, 1100, 1984
BstX2I RGATCY 3 cut(s) 1085, 1859, 2746
BstXI CCANNNNNNTGG 2 cut(s) 2264, 2513
BstYI RGATCY 3 cut(s) 1085, 1859, 2746
BsuI GTATCC 1 cut(s) 2450
BsuRI GGCC 7 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321
BtgI CCRYGG 1 cut(s) 862
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 7 cut(s) 178, 1148, 1390, 1836, 1929, 2118, 2495
BveI ACCTGC 1 cut(s) 1475
Cac8I GCNNGC 3 cut(s) 129, 1023, 2167
CaiI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
CciI TCATGA 2 cut(s) 747, 2382
CfoI GCGC 2 cut(s) 120, 400
Cfr13I GGNCC 4 cut(s) 704, 1129, 1541, 1567
CseI GACGC 2 cut(s) 1974, 2658
Csp6I GTAC 5 cut(s) 325, 867, 1735, 2070, 2226
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviQI GTAC 5 cut(s) 325, 867, 1735, 2070, 2226
EaeI YGGCCR 1 cut(s) 2266
Eam1104I CTCTTC 3 cut(s) 644, 967, 2420
EarI CTCTTC 3 cut(s) 644, 967, 2420
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 3 cut(s) 862, 1804, 2322
Eco147I AGGCCT 1 cut(s) 2321
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 2 cut(s) 1129, 1567
Eco57I CTGAAG 4 cut(s) 309, 1094, 1341, 1673
Eco72I CACGTG 1 cut(s) 839
Eco88I CYCGRG 1 cut(s) 1703
EcoO109I RGGNCCY 2 cut(s) 704, 1541
EcoRI GAATTC 3 cut(s) 226, 751, 2063
EcoRII CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 3 cut(s) 862, 1804, 2322
EcoT22I ATGCAT 4 cut(s) 567, 1291, 2692, 2760
ErhI CCWWGG 3 cut(s) 862, 1804, 2322
FalI AAGNNNNNCTT 4 cut(s) 1309, 1341, 2766, 2798
FaqI GGGAC 2 cut(s) 260, 1729
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 4 cut(s) 531, 682, 1771, 2050
Fnu4HI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
Fsp4HI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
FspBI CTAG 5 cut(s) 6, 38, 1314, 1623, 2592
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 2 cut(s) 119, 399
GluI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 1 cut(s) 456
HaeIII GGCC 7 cut(s) 705, 718, 1543, 1548, 1803, 2268, 2321
HapII CCGG 4 cut(s) 825, 1440, 1863, 2395
HgaI GACGC 2 cut(s) 1974, 2658
HhaI GCGC 2 cut(s) 120, 400
Hin1I GRCGYC 1 cut(s) 575
Hin6I GCGC 2 cut(s) 118, 398
HinP1I GCGC 2 cut(s) 118, 398
HincII GTYRAC 1 cut(s) 2335
HindII GTYRAC 1 cut(s) 2335
HpaII CCGG 4 cut(s) 825, 1440, 1863, 2395
HphI GGTGA 3 cut(s) 482, 752, 1514
Hpy166II GTNNAC 4 cut(s) 176, 1567, 2335, 2502
Hpy8I GTNNAC 4 cut(s) 176, 1567, 2335, 2502
HpyCH4III ACNGT 6 cut(s) 187, 1172, 1283, 1734, 2122, 2499
HpyCH4IV ACGT 3 cut(s) 575, 838, 2055
HpySE526I ACGT 3 cut(s) 575, 838, 2055
Hsp92I GRCGYC 1 cut(s) 575
HspAI GCGC 2 cut(s) 118, 398
Kpn2I TCCGGA 1 cut(s) 1862
KpnI GGTACC 1 cut(s) 870
Ksp22I TGATCA 4 cut(s) 531, 682, 1771, 2050
LguI GCTCTTC 1 cut(s) 967
LmnI GCTCC 3 cut(s) 112, 827, 838
Lsp1109I GCAGC 9 cut(s) 127, 505, 842, 1173, 1201, 1204, 1423, 1619, 2504
LweI GCATC 7 cut(s) 67, 899, 1298, 1390, 1471, 1945, 2599
MaeI CTAG 5 cut(s) 6, 38, 1314, 1623, 2592
MaeII ACGT 3 cut(s) 575, 838, 2055
MaeIII GTNAC 3 cut(s) 269, 1502, 2491
MflI RGATCY 3 cut(s) 1085, 1859, 2746
MhlI GDGCHC 2 cut(s) 178, 1390
MlsI TGGCCA 1 cut(s) 2268
MluNI TGGCCA 1 cut(s) 2268
MlyI GAGTC 2 cut(s) 1961, 2635
MmeI TCCRAC 1 cut(s) 1837
Mox20I TGGCCA 1 cut(s) 2268
Mph1103I ATGCAT 4 cut(s) 567, 1291, 2692, 2760
MroI TCCGGA 1 cut(s) 1862
MroXI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
MscI TGGCCA 1 cut(s) 2268
MseI TTAA 4 cut(s) 1067, 1586, 1751, 1902
MslI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2636
Msp20I TGGCCA 1 cut(s) 2268
MspA1I CMGCKG 1 cut(s) 496
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 4 cut(s) 825, 1440, 1863, 2395
MspR9I CCNGG 7 cut(s) 1397, 1413, 1441, 1545, 1821, 1945, 2396
Mva1269I GAATGC 3 cut(s) 858, 2545, 2690
MvaI CCWGG 5 cut(s) 1397, 1413, 1545, 1821, 1945
NciI CCSGG 2 cut(s) 1441, 2396
NcoI CCATGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 1798
NlaIV GGNNCC 3 cut(s) 868, 1569, 1861
NmuCI GTSAC 2 cut(s) 1502, 2491
NsbI TGCGCA 1 cut(s) 399
NsiI ATGCAT 4 cut(s) 567, 1291, 2692, 2760
NspI RCATGY 3 cut(s) 1304, 1985, 2762
NspV TTCGAA 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 1703
PagI TCATGA 2 cut(s) 747, 2382
PceI AGGCCT 1 cut(s) 2321
PciSI GCTCTTC 1 cut(s) 967
PctI GAATGC 3 cut(s) 858, 2545, 2690
PdmI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
PflMI CCANNNNNTGG 1 cut(s) 1799
PfoI TCCNGGA 2 cut(s) 1819, 2394
PkrI GCNGC 9 cut(s) 117, 495, 832, 1188, 1191, 1194, 1438, 1609, 2519
PleI GAGTC 2 cut(s) 1961, 2635
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 2 cut(s) 1961, 2635
Ppu21I YACGTR 2 cut(s) 839, 2056
PshAI GACNNNNGTC 2 cut(s) 574, 1984
Psp6I CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
PspCI CACGTG 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 5 cut(s) 1395, 1411, 1543, 1819, 1943
PspN4I GGNNCC 3 cut(s) 868, 1569, 1861
PspPI GGNCC 4 cut(s) 704, 1129, 1541, 1567
PspXI VCTCGAGB 1 cut(s) 1703
PstI CTGCAG 2 cut(s) 1093, 1191
PstNI CAGNNNCTG 4 cut(s) 1186, 1198, 1944, 1958
PsuI RGATCY 3 cut(s) 1085, 1859, 2746
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 5 cut(s) 326, 868, 1736, 2071, 2227
RsaNI GTAC 5 cut(s) 325, 867, 1735, 2070, 2226
RseI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2636
SapI GCTCTTC 1 cut(s) 967
SaqAI TTAA 4 cut(s) 1067, 1586, 1751, 1902
SatI GCNGC 9 cut(s) 116, 494, 831, 1187, 1190, 1193, 1437, 1608, 2518
Sau96I GGNCC 4 cut(s) 704, 1129, 1541, 1567
SchI GAGTC 2 cut(s) 1961, 2635
ScrFI CCNGG 7 cut(s) 1397, 1413, 1441, 1545, 1821, 1945, 2396
SduI GDGCHC 2 cut(s) 178, 1390
SfaNI GCATC 7 cut(s) 67, 899, 1298, 1390, 1471, 1945, 2599
SfcI CTRYAG 5 cut(s) 668, 1089, 1187, 2121, 2614
Sfr274I CTCGAG 1 cut(s) 1703
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 2 cut(s) 1129, 1567
SlaI CTCGAG 1 cut(s) 1703
SmiMI CAYNNNNRTG 6 cut(s) 583, 1479, 1953, 2174, 2511, 2636
SmlI CTYRAG 4 cut(s) 95, 1066, 1703, 2445
SmoI CTYRAG 4 cut(s) 95, 1066, 1703, 2445
SpeI ACTAGT 1 cut(s) 2591
SseBI AGGCCT 1 cut(s) 2321
SsiI CCGC 2 cut(s) 179, 365
SspI AATATT 1 cut(s) 2142
SspMI CTAG 5 cut(s) 6, 38, 1314, 1623, 2592
StuI AGGCCT 1 cut(s) 2321
StyD4I CCNGG 7 cut(s) 1395, 1411, 1439, 1543, 1819, 1943, 2394
StyI CCWWGG 3 cut(s) 862, 1804, 2322
TaaI ACNGT 6 cut(s) 187, 1172, 1283, 1734, 2122, 2499
TaiI ACGT 3 cut(s) 578, 841, 2058
TaqI TCGA 5 cut(s) 602, 1479, 1704, 2109, 2370
Tru1I TTAA 4 cut(s) 1067, 1586, 1751, 1902
Tru9I TTAA 4 cut(s) 1067, 1586, 1751, 1902
TscAI CASTG 7 cut(s) 178, 1148, 1390, 1836, 1929, 2125, 2502
TseFI GTSAC 2 cut(s) 1502, 2491
TseI GCWGC 9 cut(s) 115, 493, 830, 1186, 1189, 1192, 1436, 1607, 2517
Tsp45I GTSAC 2 cut(s) 1502, 2491
TspGWI ACGGA 1 cut(s) 1521
TspRI CASTG 7 cut(s) 178, 1148, 1390, 1836, 1929, 2125, 2502
Van91I CCANNNNNTGG 1 cut(s) 1799
Vha464I CTTAAG 1 cut(s) 1066
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 1129, 1567
XapI RAATTY 9 cut(s) 67, 226, 536, 598, 751, 1054, 2063, 2210, 2454
XceI RCATGY 3 cut(s) 1304, 1985, 2762
XhoI CTCGAG 1 cut(s) 1703
XmnI GAANNNNTTC 3 cut(s) 1321, 1324, 2141
XspI CTAG 5 cut(s) 6, 38, 1314, 1623, 2592
ZraI GACGTC 1 cut(s) 576
Zsp2I ATGCAT 4 cut(s) 567, 1291, 2692, 2760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.