pycom07g16540

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007939
Physical Location & Seq
Forward (+)
18971167 .. 18972430
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16540.3

Sequence Viewer

Length: 1029 bp
ATGCCAAATGGGCATAACCAGAGGCTGCAGCAGGAACTGTTAGAGAAGGCAAATGCTGAAATTTTTTCTCTTCCTACTAATTATGCTCATCTAGCAAAGAAAAATGGAATCATAACAAACCGTACTACATCCGCTGGGCAAGCTAATTTTTCAAGAAGAACTTCTTCTCCCAGTCTTTACTCAAACGGGAGTGCTTCAGGCTTTGGAGCTTTGGAGGGATCTAATGTTCCTTACCAAGAAGCAAATACGCCAGGTTTGGACTTTGATGGCCATGTTGCTGGGGCCTATCCTGTAAATCCGAAGTTGAATATGAAAATTAATGATCATCTTGATGCAGTAGCTAGAGGTGATGAACAAGGTCTGAACAGACTGGGAAACAAAGTAGGGCCTGGTCTCCATTCTTCTGTTATGGACCCATCTTATATTCAGTTTTTGCAAAGAACTGATTATGCAACACGTAATGTAACTAGTCCAAGTGGTTATCCTCTGAGTAAAAACCACTTTGGTACTTTGCATGGAGACTTAGAGGGGCTTCAGAAGGCATATCTTGAGGCATTGCTTGTTCAAAGGAAGCAACAGTATGAGTTGTCACTTTTAGGTAAATCTGGTGGTTTTAATCATGGTTATTATGGGAACCCACCCTATGGGCTTGGTATGTATCCTGGAAATCCAATGGAAAATTATCTGCATCCTTCTATTGGGCCTGGTAGTCCAATGTTTGAGAGTGAGAAGATTGCACGCTTTAATTCTATGTTGAGAAGTTCAATGGGAGGGTCAGTTAGCTCCTGGAACTCAGACATTGGAAAGGACCTAGAACGACGACATGCATCATCATTATTAGATGAATTCAAGAACAACAAGAACAAGTCTTTTGAACTTGCAGACATTGTTGATCGATCATGTAGTGGAATTCAGTACCGACCAGTATGGAAGTCGCTTTATTCAGCAGAAACTAGAAACTGCCACTGTGGAAGAAAAAATGAAGATATTTCCAGAGATTATTCCTCATGTTCGCACCTTGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

343

Amino Acids

37.68

Weight (kDa)

7.73

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 644
AciI CCGC 1 cut(s) 132
AclWI GGATC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 3 cut(s) 60, 845, 909
AcuI CTGAAG 2 cut(s) 180, 518
AfaI GTAC 3 cut(s) 124, 508, 917
AfiI CCNNNNNNNGG 2 cut(s) 644, 698
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 6 cut(s) 153, 307, 566, 765, 850, 875
AhlI ACTAGT 1 cut(s) 467
AjnI CCWGG 5 cut(s) 250, 388, 661, 703, 785
AloI GAACNNNNNNTCC 4 cut(s) 151, 183, 210, 242
AluBI AGCT 4 cut(s) 143, 209, 341, 783
AluI AGCT 4 cut(s) 143, 209, 341, 783
Alw26I GTCTC 2 cut(s) 398, 513
AlwI GGATC 1 cut(s) 226
AlwNI CAGNNNCTG 2 cut(s) 25, 37
AoxI GGCC 4 cut(s) 268, 282, 386, 701
ApeKI GCWGC 2 cut(s) 25, 28
ApoI RAATTY 3 cut(s) 60, 845, 909
AseI ATTAAT 1 cut(s) 318
Asp700I GAANNNNTTC 2 cut(s) 160, 163
AspS9I GGNCC 5 cut(s) 282, 386, 412, 701, 808
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 2 cut(s) 412, 808
BalI TGGCCA 1 cut(s) 270
BbvI GCAGC 2 cut(s) 12, 40
BccI CCATC 2 cut(s) 260, 424
BciT130I CCWGG 5 cut(s) 252, 390, 663, 705, 787
BciVI GTATCC 1 cut(s) 669
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 398, 513
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 5 cut(s) 92, 342, 468, 812, 954
BfmI CTRYAG 1 cut(s) 26
BfuI GTATCC 1 cut(s) 669
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 2 cut(s) 26, 29
BlsI GCNGC 2 cut(s) 27, 30
Bme1390I CCNGG 5 cut(s) 252, 390, 663, 705, 787
Bme18I GGWCC 2 cut(s) 412, 808
BmgT120I GGNCC 5 cut(s) 282, 386, 412, 701, 808
BmiI GGNNCC 3 cut(s) 283, 414, 635
BmrFI CCNGG 5 cut(s) 252, 390, 663, 705, 787
BmrI ACTGGG 2 cut(s) 165, 380
BmsI GCATC 3 cut(s) 322, 697, 836
BmuI ACTGGG 2 cut(s) 165, 380
BpuEI CTTGAG 1 cut(s) 569
Bsa29I ATCGAT 1 cut(s) 895
BsaAI YACGTR 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 398
BsaXI ACNNNNNCTCC 4 cut(s) 151, 181, 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 644, 698
Bse1I ACTGG 3 cut(s) 171, 375, 923
Bse3DI GCAATG 1 cut(s) 554
BseBI CCWGG 5 cut(s) 252, 390, 663, 705, 787
BseCI ATCGAT 1 cut(s) 895
BseGI GGATG 2 cut(s) 128, 688
BseLI CCNNNNNNNGG 2 cut(s) 644, 698
BseMI GCAATG 1 cut(s) 554
BseMII CTCAG 2 cut(s) 479, 807
BseNI ACTGG 3 cut(s) 171, 375, 923
BseXI GCAGC 2 cut(s) 12, 40
BseYI CCCAGC 2 cut(s) 134, 278
BshFI GGCC 4 cut(s) 270, 284, 388, 703
BshVI ATCGAT 1 cut(s) 895
BslI CCNNNNNNNGG 2 cut(s) 644, 698
BsmAI GTCTC 2 cut(s) 398, 513
BsnI GGCC 4 cut(s) 270, 284, 388, 703
Bso31I GGTCTC 1 cut(s) 398
Bsp143I GATC 4 cut(s) 218, 322, 892, 896
BspACI CCGC 1 cut(s) 132
BspANI GGCC 4 cut(s) 270, 284, 388, 703
BspCNI CTCAG 2 cut(s) 480, 806
BspDI ATCGAT 1 cut(s) 895
BspLI GGNNCC 3 cut(s) 283, 414, 635
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 554
BsrI ACTGG 3 cut(s) 171, 375, 923
BssMI GATC 4 cut(s) 218, 322, 892, 896
Bst2UI CCWGG 5 cut(s) 252, 390, 663, 705, 787
Bst4CI ACNGT 4 cut(s) 39, 122, 579, 968
Bst6I CTCTTC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 141, 739
BstDEI CTNAG 3 cut(s) 488, 523, 793
BstF5I GGATG 2 cut(s) 128, 688
BstKTI GATC 4 cut(s) 221, 325, 895, 899
BstMAI GTCTC 2 cut(s) 398, 513
BstMBI GATC 4 cut(s) 218, 322, 892, 896
BstMWI GCNNNNNNNGC 3 cut(s) 10, 92, 140
BstNI CCWGG 5 cut(s) 252, 390, 663, 705, 787
BstNSI RCATGY 1 cut(s) 827
BstSCI CCNGG 5 cut(s) 250, 388, 661, 703, 785
BstSFI CTRYAG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 12, 40
BstX2I RGATCY 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 278
BstYI RGATCY 1 cut(s) 218
Bsu15I ATCGAT 1 cut(s) 895
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 4 cut(s) 270, 284, 388, 703
BsuTUI ATCGAT 1 cut(s) 895
BtsCI GGATG 2 cut(s) 128, 688
BtsIMutI CAGTG 1 cut(s) 964
Cac8I GCNNGC 2 cut(s) 141, 739
CaiI CAGNNNCTG 2 cut(s) 25, 37
Cfr13I GGNCC 5 cut(s) 282, 386, 412, 701, 808
ClaI ATCGAT 1 cut(s) 895
Csp6I GTAC 3 cut(s) 123, 507, 916
CviAII CATG 6 cut(s) 272, 515, 620, 824, 900, 1008
CviQI GTAC 3 cut(s) 123, 507, 916
DdeI CTNAG 3 cut(s) 488, 523, 793
DpnI GATC 4 cut(s) 220, 324, 894, 898
DpnII GATC 4 cut(s) 218, 322, 892, 896
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 412, 808
Eco57I CTGAAG 2 cut(s) 180, 518
EcoO109I RGGNCCY 3 cut(s) 282, 386, 808
EcoRI GAATTC 2 cut(s) 845, 909
EcoRII CCWGG 5 cut(s) 250, 388, 661, 703, 785
EcoT22I ATGCAT 1 cut(s) 829
FaeI CATG 6 cut(s) 275, 518, 623, 827, 903, 1011
FalI AAGNNNNNCTT 4 cut(s) 145, 177, 148, 180
FatI CATG 6 cut(s) 271, 514, 619, 823, 899, 1007
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 26, 29
FokI GGATG 2 cut(s) 115, 675
Fsp4HI GCNGC 2 cut(s) 26, 29
FspBI CTAG 5 cut(s) 92, 342, 468, 812, 954
GluI GCNGC 2 cut(s) 26, 29
GsaI CCCAGC 2 cut(s) 138, 282
HaeIII GGCC 4 cut(s) 270, 284, 388, 703
Hin1II CATG 6 cut(s) 275, 518, 623, 827, 903, 1011
HinfI GANTC 1 cut(s) 108
HphI GGTGA 1 cut(s) 359
Hpy188I TCNGA 5 cut(s) 300, 363, 489, 537, 796
Hpy188III TCNNGA 5 cut(s) 153, 329, 548, 850, 993
Hpy99I CGWCG 1 cut(s) 822
HpyAV CCTTC 3 cut(s) 40, 532, 702
HpyCH4III ACNGT 4 cut(s) 39, 122, 579, 968
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 9 cut(s) 28, 335, 436, 452, 514, 688, 737, 827, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 92, 140
HpyF3I CTNAG 3 cut(s) 488, 523, 793
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 6 cut(s) 275, 518, 623, 827, 903, 1011
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 4 cut(s) 218, 322, 892, 896
LmnI GCTCC 2 cut(s) 206, 788
Lsp1109I GCAGC 2 cut(s) 12, 40
LweI GCATC 3 cut(s) 322, 697, 836
MaeI CTAG 5 cut(s) 92, 342, 468, 812, 954
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 2 cut(s) 463, 588
MalI GATC 4 cut(s) 220, 324, 894, 898
MboI GATC 4 cut(s) 218, 322, 892, 896
MboII GAAGA 7 cut(s) 62, 156, 168, 393, 742, 984, 995
MflI RGATCY 1 cut(s) 218
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 8 cut(s) 60, 79, 145, 315, 679, 745, 845, 909
MluNI TGGCCA 1 cut(s) 270
MnlI CCTC 8 cut(s) 15, 208, 338, 495, 520, 544, 764, 1015
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 829
MroXI GAANNNNTTC 2 cut(s) 160, 163
MscI TGGCCA 1 cut(s) 270
MseI TTAA 3 cut(s) 318, 615, 744
MslI CAYNNNNRTG 2 cut(s) 330, 1020
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 134
MspR9I CCNGG 5 cut(s) 252, 390, 663, 705, 787
MvaI CCWGG 5 cut(s) 252, 390, 663, 705, 787
MwoI GCNNNNNNNGC 3 cut(s) 10, 92, 140
NdeII GATC 4 cut(s) 218, 322, 892, 896
NlaIII CATG 6 cut(s) 275, 518, 623, 827, 903, 1011
NlaIV GGNNCC 3 cut(s) 283, 414, 635
NmuCI GTSAC 1 cut(s) 588
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 1 cut(s) 827
PdmI GAANNNNTTC 2 cut(s) 160, 163
PfeI GAWTC 1 cut(s) 108
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 2 cut(s) 661, 785
PkrI GCNGC 2 cut(s) 27, 30
Ppu21I YACGTR 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 808
PshBI ATTAAT 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 5 cut(s) 250, 388, 661, 703, 785
PspFI CCCAGC 2 cut(s) 134, 278
PspGI CCWGG 5 cut(s) 250, 388, 661, 703, 785
PspN4I GGNNCC 3 cut(s) 283, 414, 635
PspPI GGNCC 5 cut(s) 282, 386, 412, 701, 808
PspPPI RGGWCCY 1 cut(s) 808
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 2 cut(s) 25, 37
PsuI RGATCY 1 cut(s) 218
RsaI GTAC 3 cut(s) 124, 508, 917
RsaNI GTAC 3 cut(s) 123, 507, 916
RseI CAYNNNNRTG 2 cut(s) 330, 1020
SaqAI TTAA 3 cut(s) 318, 615, 744
SatI GCNGC 2 cut(s) 26, 29
Sau3AI GATC 4 cut(s) 218, 322, 892, 896
Sau96I GGNCC 5 cut(s) 282, 386, 412, 701, 808
ScrFI CCNGG 5 cut(s) 252, 390, 663, 705, 787
SfaNI GCATC 3 cut(s) 322, 697, 836
SfcI CTRYAG 1 cut(s) 26
SinI GGWCC 2 cut(s) 412, 808
SmiMI CAYNNNNRTG 2 cut(s) 330, 1020
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 8 cut(s) 60, 79, 145, 315, 679, 745, 845, 909
SsiI CCGC 1 cut(s) 132
SspMI CTAG 5 cut(s) 92, 342, 468, 812, 954
StyD4I CCNGG 5 cut(s) 250, 388, 661, 703, 785
TaaI ACNGT 4 cut(s) 39, 122, 579, 968
TaiI ACGT 1 cut(s) 460
TaqI TCGA 1 cut(s) 895
TasI AATT 8 cut(s) 60, 79, 145, 315, 679, 745, 845, 909
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 3 cut(s) 318, 615, 744
Tru9I TTAA 3 cut(s) 318, 615, 744
TscAI CASTG 1 cut(s) 971
TseFI GTSAC 1 cut(s) 588
TseI GCWGC 2 cut(s) 25, 28
Tsp45I GTSAC 1 cut(s) 588
TspDTI ATGAA 4 cut(s) 326, 366, 858, 996
TspRI CASTG 1 cut(s) 971
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 2 cut(s) 412, 808
VspI ATTAAT 1 cut(s) 318
XapI RAATTY 3 cut(s) 60, 845, 909
XceI RCATGY 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 160, 163
XspI CTAG 5 cut(s) 92, 342, 468, 812, 954
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.