FvH4_7g19800

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009503
Physical Location & Seq
Reverse (-)
16260371 .. 16262954
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19800.t1

Sequence Viewer

Length: 849 bp
ATGTATAGGTTGAGCAACACAGTGATTGGGTTCTTGAACCTCTTCACTCTTTTAGCATCAATACCTATCATTGGTGGTGGTCTATGGATGGCAAGGAGCAGCACAACATGTGAAAGCTTCCTCCAAACCCCACTCTTAGTGATTGGTTTTGTTGTGCTGATCATATCTCTAGCTGGGTTCATTGGTGCTTGTTTCCATGTTGCTTGGGCGCTTTGGGTGTACTTAGTAGTCATGCTGTTCCTTATTGCCACCCTGATGGGGTTTACTGCGTTTGGGTTTGTGGTTACAAGTCAAGGTGCTGGAGTTGATGTGCCTGGTAGGGCTTATAAGGAATATCATCTTGAAGATTACTCACCATGGTTGAGGAAGAGGATTAAGGACCCCAATTACTGGAGCAACATTAGGAGTTGTATTTTGGGGTCTAAGACTTGTGCAAAGCTTCTTGTTTGGACTCCTCTTGATTATCTTGAGAGGGACATGTCTCCAATACAGTCTGGTTGTTGCAAGCCACCAACAGCATGCAACTACAACATGGCAACAACAGTGAGTCAAGATCCGGATTGCTACCGGTGGAACAATGCCCCGAATTTGTTATGCTACGAATGTGATTCATGCAAGGCAGGAGTGCTCGAAGATATCAAAAGGGACTGGCACAAGCTCTCTGTCCTCAACATTGTGATGATTGTTGTCCTCATTGGAATATATTCCATTGGATGCTGTGCTTTCCAAAACACAAAAAGAGCTGAAACGGATTACCCCTATGGCCAGAACCGGATGTCCAAAGTCCGACCAAGATGGGATTACCACTGGTGGAGATGGTGGCATCACAGAAGAGAACAGCTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

283

Amino Acids

32.42

Weight (kDa)

8.75

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 241 4.5e-36 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 556
AclWI GGATC 1 cut(s) 548
AcoI YGGCCR 1 cut(s) 763
AcsI RAATTY 1 cut(s) 586
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 71, 258, 390
AflIII ACRYGT 2 cut(s) 107, 477
AgeI ACCGGT 1 cut(s) 567
AgsI TTSAA 2 cut(s) 37, 344
AjnI CCWGG 1 cut(s) 313
AloI GAACNNNNNNTCC 2 cut(s) 761, 793
AluBI AGCT 6 cut(s) 117, 173, 439, 658, 743, 841
AluI AGCT 6 cut(s) 117, 173, 439, 658, 743, 841
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 586
AsiGI ACCGGT 1 cut(s) 567
Asp700I GAANNNNTTC 2 cut(s) 41, 703
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 345
AvaII GGWCC 1 cut(s) 379
BalI TGGCCA 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 111
BccI CCATC 4 cut(s) 82, 250, 789, 810
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 2 cut(s) 170, 847
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 65, 704, 832
BpmI CTGGAG 2 cut(s) 321, 412
BpuEI CTTGAG 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 356
BsaWI WCCGGW 3 cut(s) 556, 567, 771
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 258, 390
Bse118I RCCGGY 1 cut(s) 567
Bse1I ACTGG 3 cut(s) 395, 653, 812
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 3 cut(s) 93, 719, 780
BseLI CCNNNNNNNGG 3 cut(s) 71, 258, 390
BseNI ACTGG 3 cut(s) 395, 653, 812
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 765
BshTI ACCGGT 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 630
BsiSI CCGG 3 cut(s) 557, 568, 772
BslFI GGGAC 2 cut(s) 488, 659
BslI CCNNNNNNNGG 3 cut(s) 71, 258, 390
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 2 cut(s) 488, 659
BsnI GGCC 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 630
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 2 cut(s) 159, 553
Bsp19I CCATGG 1 cut(s) 356
BspANI GGCC 1 cut(s) 765
BspEI TCCGGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 381
BspPI GGATC 1 cut(s) 548
BsrFI RCCGGY 1 cut(s) 567
BsrI ACTGG 3 cut(s) 395, 653, 812
BssAI RCCGGY 1 cut(s) 567
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 2 cut(s) 159, 553
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 22, 492, 544
Bst6I CTCTTC 3 cut(s) 47, 362, 826
BstC8I GCNNGC 2 cut(s) 506, 520
BstDEI CTNAG 3 cut(s) 136, 223, 423
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 3 cut(s) 93, 719, 780
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 2 cut(s) 162, 556
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 159, 553
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 3 cut(s) 111, 481, 522
BstSCI CCNGG 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 1 cut(s) 765
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 3 cut(s) 93, 719, 780
BtsIMutI CAGTG 3 cut(s) 27, 549, 805
Cac8I GCNNGC 2 cut(s) 506, 520
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 220
CspAI ACCGGT 1 cut(s) 567
CviAII CATG 8 cut(s) 108, 197, 232, 357, 478, 519, 532, 612
CviJI RGCY 9 cut(s) 117, 173, 323, 439, 508, 658, 743, 765, 841
CviKI_1 RGCY 9 cut(s) 117, 173, 323, 439, 508, 658, 743, 765, 841
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 136, 223, 423
DpnI GATC 2 cut(s) 161, 555
DpnII GATC 2 cut(s) 159, 553
EaeI YGGCCR 1 cut(s) 763
Eam1104I CTCTTC 3 cut(s) 47, 362, 826
EarI CTCTTC 3 cut(s) 47, 362, 826
Eco130I CCWWGG 1 cut(s) 356
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 8 cut(s) 111, 200, 235, 360, 481, 522, 535, 615
FaqI GGGAC 2 cut(s) 488, 659
FatI CATG 8 cut(s) 107, 196, 231, 356, 477, 518, 531, 611
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 3 cut(s) 100, 726, 787
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 2 cut(s) 170, 847
GlaI GCGC 1 cut(s) 210
GluI GCNGC 1 cut(s) 100
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 321, 412
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 1 cut(s) 765
HapII CCGG 3 cut(s) 557, 568, 772
HhaI GCGC 1 cut(s) 211
Hin1II CATG 8 cut(s) 111, 200, 235, 360, 481, 522, 535, 615
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 2 cut(s) 115, 437
HinfI GANTC 3 cut(s) 451, 547, 608
HpaII CCGG 3 cut(s) 557, 568, 772
HphI GGTGA 1 cut(s) 345
Hpy166II GTNNAC 2 cut(s) 220, 264
Hpy188I TCNGA 1 cut(s) 788
Hpy188III TCNNGA 6 cut(s) 34, 341, 458, 467, 551, 557
Hpy8I GTNNAC 2 cut(s) 220, 264
HpyCH4III ACNGT 3 cut(s) 22, 492, 544
HpyCH4V TGCA 4 cut(s) 434, 504, 522, 615
HpyF3I CTNAG 3 cut(s) 136, 223, 423
Hsp92II CATG 8 cut(s) 111, 200, 235, 360, 481, 522, 535, 615
HspAI GCGC 1 cut(s) 209
Kpn2I TCCGGA 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 2 cut(s) 159, 553
LmnI GCTCC 2 cut(s) 96, 393
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 3 cut(s) 65, 704, 832
MaeI CTAG 2 cut(s) 170, 847
MaeIII GTNAC 1 cut(s) 283
MalI GATC 2 cut(s) 161, 555
MboI GATC 2 cut(s) 159, 553
MboII GAAGA 5 cut(s) 34, 356, 379, 644, 843
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 630
MlsI TGGCCA 1 cut(s) 765
MluCI AATT 2 cut(s) 385, 586
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 2 cut(s) 445, 556
MmeI TCCRAC 1 cut(s) 811
MnlI CCTC 8 cut(s) 50, 131, 357, 363, 465, 465, 677, 701
Mox20I TGGCCA 1 cut(s) 765
MroI TCCGGA 1 cut(s) 556
MroXI GAANNNNTTC 2 cut(s) 41, 703
MscI TGGCCA 1 cut(s) 765
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 254, 677
Msp20I TGGCCA 1 cut(s) 765
MspI CCGG 3 cut(s) 557, 568, 772
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 356
NdeII GATC 2 cut(s) 159, 553
NlaIII CATG 8 cut(s) 111, 200, 235, 360, 481, 522, 535, 615
NlaIV GGNNCC 1 cut(s) 381
NspI RCATGY 3 cut(s) 111, 481, 522
PaeI GCATGC 1 cut(s) 522
PciI ACATGT 2 cut(s) 107, 477
PdmI GAANNNNTTC 2 cut(s) 41, 703
PfeI GAWTC 1 cut(s) 608
PflMI CCANNNNNTGG 1 cut(s) 390
PinAI ACCGGT 1 cut(s) 567
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 2 cut(s) 445, 555
PpsI GAGTC 2 cut(s) 445, 555
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 2 cut(s) 107, 477
PsiI TTATAA 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 2 cut(s) 254, 677
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 2 cut(s) 159, 553
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 2 cut(s) 445, 556
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 3 cut(s) 65, 704, 832
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 2 cut(s) 254, 677
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SphI GCATGC 1 cut(s) 522
Sse9I AATT 2 cut(s) 385, 586
SspMI CTAG 2 cut(s) 170, 847
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 3 cut(s) 22, 492, 544
TaqI TCGA 1 cut(s) 630
TasI AATT 2 cut(s) 385, 586
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 3 cut(s) 27, 549, 812
TseI GCWGC 1 cut(s) 99
TspDTI ATGAA 2 cut(s) 169, 600
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 3 cut(s) 27, 549, 812
Van91I CCANNNNNTGG 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 1 cut(s) 586
XceI RCATGY 3 cut(s) 111, 481, 522
XmnI GAANNNNTTC 2 cut(s) 41, 703
XspI CTAG 2 cut(s) 170, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.