FvH4_7g19830

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009503
Physical Location & Seq
Reverse (-)
16277694 .. 16278360
667 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19830.t1

Sequence Viewer

Length: 498 bp
ATGTATAGGTTGAGCAACACAGTGATTGGGTTCTTGAACCTCTTCACTCTTTTAGCATCAATACCTATCATTGGTGGTGGTCTATGGATGGCAAGGAGCAGCACAACATGTGAAAGCTTCCTCCAAACCCCACTCTTAGTGATTGGTTTTGTTGTGCTGATCATATCTCTAGCCGGGTTCGTTGGTGCTTGTTTCCATGTTGCTTGGGCGCTTTGGGTGTACTTAGTAGTCATGCTGTTCCTTATTGCCACCCTGATGGGGTTTACTGTGTTTGGGTTTGTGGTTACAAGTCAAGGTGCTGGAGTTGATGTGCCTGGTAGGGCTTATAAGGAATATCATCTTGAAGATTACTCACCATGGTTGAGGAAGAGGATTAAGGACCCCAATTACTGGAGCACATTAGGAGTTGTATTTTGGGGTCTAAGACTTGTGCAAAGCTTCTTGTTTGGACTCCTCTTGATTATCTTGAGAGGGACATGTCTCCAATACAGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

166

Amino Acids

18.52

Weight (kDa)

9.37

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 127 1.8e-23 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 390
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 71, 258, 390
AflIII ACRYGT 2 cut(s) 107, 476
AgsI TTSAA 2 cut(s) 37, 344
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 2 cut(s) 117, 438
AluI AGCT 2 cut(s) 117, 438
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 485
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 1 cut(s) 99
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 345
AvaII GGWCC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 111
BccI CCATC 2 cut(s) 82, 250
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 1 cut(s) 170
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 2 cut(s) 175, 315
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 2 cut(s) 175, 315
BmsI GCATC 1 cut(s) 65
BpmI CTGGAG 2 cut(s) 321, 412
BpuEI CTTGAG 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 258, 390
Bse1I ACTGG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 71, 258, 390
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 111
BshFI GGCC 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 398
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 3 cut(s) 71, 258, 390
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 1 cut(s) 159
Bsp19I CCATGG 1 cut(s) 356
BspANI GGCC 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 381
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 22, 268
Bst6I CTCTTC 2 cut(s) 47, 362
BstDEI CTNAG 3 cut(s) 136, 223, 422
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 1 cut(s) 162
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 111, 480
BstSCI CCNGG 2 cut(s) 173, 313
BstV1I GCAGC 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 27
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 220
CviAII CATG 6 cut(s) 108, 197, 232, 357, 477, 495
CviJI RGCY 5 cut(s) 117, 173, 323, 438, 493
CviKI_1 RGCY 5 cut(s) 117, 173, 323, 438, 493
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 136, 223, 422
DpnI GATC 1 cut(s) 161
DpnII GATC 1 cut(s) 159
Eam1104I CTCTTC 2 cut(s) 47, 362
EarI CTCTTC 2 cut(s) 47, 362
Eco130I CCWWGG 1 cut(s) 356
Eco47I GGWCC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 6 cut(s) 111, 200, 235, 360, 480, 498
FaqI GGGAC 1 cut(s) 487
FatI CATG 6 cut(s) 107, 196, 231, 356, 476, 494
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 210
GluI GCNGC 1 cut(s) 100
GsuI CTGGAG 2 cut(s) 321, 412
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 1 cut(s) 493
HapII CCGG 1 cut(s) 174
HhaI GCGC 1 cut(s) 211
Hin1II CATG 6 cut(s) 111, 200, 235, 360, 480, 498
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 2 cut(s) 115, 436
HinfI GANTC 1 cut(s) 450
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 345
Hpy166II GTNNAC 2 cut(s) 220, 264
Hpy188III TCNNGA 4 cut(s) 34, 341, 457, 466
Hpy8I GTNNAC 2 cut(s) 220, 264
HpyCH4III ACNGT 2 cut(s) 22, 268
HpyCH4V TGCA 1 cut(s) 433
HpyF3I CTNAG 3 cut(s) 136, 223, 422
Hsp92II CATG 6 cut(s) 111, 200, 235, 360, 480, 498
HspAI GCGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 96, 393
LpnPI CCDG 7 cut(s) 187, 266, 285, 300, 327, 376, 475
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 1 cut(s) 65
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 161
MboI GATC 1 cut(s) 159
MboII GAAGA 3 cut(s) 34, 356, 379
MhlI GDGCHC 1 cut(s) 398
MluCI AATT 1 cut(s) 385
MlyI GAGTC 1 cut(s) 444
MnlI CCTC 6 cut(s) 50, 131, 357, 363, 464, 464
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 175, 315
MvaI CCWGG 1 cut(s) 315
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 356
NdeII GATC 1 cut(s) 159
NlaIII CATG 6 cut(s) 111, 200, 235, 360, 480, 498
NlaIV GGNNCC 1 cut(s) 381
NspI RCATGY 2 cut(s) 111, 480
PciI ACATGT 2 cut(s) 107, 476
PdmI GAANNNNTTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 390
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 2 cut(s) 107, 476
PsiI TTATAA 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 1 cut(s) 159
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 2 cut(s) 175, 315
SduI GDGCHC 1 cut(s) 398
SetI ASST 6 cut(s) 11, 42, 67, 119, 298, 440
SfaNI GCATC 1 cut(s) 65
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 1 cut(s) 385
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 173, 313
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 22, 268
TasI AATT 1 cut(s) 385
TatI WGTACW 1 cut(s) 219
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 27
TseI GCWGC 1 cut(s) 99
TspRI CASTG 1 cut(s) 27
Van91I CCANNNNNTGG 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 379
XceI RCATGY 2 cut(s) 111, 480
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.