MD01G1110500.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009503
Physical Location & Seq
Reverse (-)
22315391 .. 22317690
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1110500.v1.1.491

Sequence Viewer

Length: 849 bp
ATGTACAGGTTCAGTAACACAGTGATAGGGTTCTTGAACCTCGTCACTCTCTTAGCATCAATTCCGATAATCGCCGCAGGTCTATGGATGTCAAGGAGCAGCACCACATGTGAAACTTTCCTCCAAACTCCACTTTTGGTGGTAGGTTTTGTGGTGCTCGTGGTATCCCTAGCTGGGTTCATTGGTGCCTGCTTCCATGTGGTATGGGCACTCTGGGTGTACTTGGCGGTGATGCTGCTGCTGATCGCAACCCTAATCGGTTTGACAGTGTTTGGGTTTGCGGTTACAAGCAAAGGTGCTGGAGTGGAGGTGCCTGGTAGGGTTTATAAGGAATACCACCTTGAGGATTACTCACCATGGTTGAGGAATAGGATTAAGGATCCTAATTATTGGAGTAAGATTAGGAGTTGCATCATGGGGTCCAAAACTTGTGCTAAGATTGTTGCTTGGACACCTACTGATTATCTTGAAAGGGACATGTCTCCAATACAGTCTGGTTGTTGCAAGCCTCCAACTTCATGTAATTACGACATGGCAACCACAGCGACTCAAGACCAAGATTGCTACCGTTGGAACAATGCGCCCAATTTGTTATGCTATGAGTGTGATTCATGCAAAGCTGGAGTTCTTGAAGATATCAAGAGGGACTGGCACAAGATCTCTGTCCTCACCATTGTCATGGTGGTTGTTCTCATTGGAGTATATTCAATTGGTTGCTGTGCTTTCCGCAACGCAGAACGAGCTGAAACGGATTATCCATATGGTCATAACGGGATGAGTAAAATCCGTCCCCGATGGGATTACCATTGGTGGAGAAAGTGGCATCACAGAAGAGAGCAGCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

283

Amino Acids

32.2

Weight (kDa)

8.63

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 241 7.9e-37 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 68
AccB1I GGYRCC 2 cut(s) 185, 310
AciI CCGC 4 cut(s) 75, 227, 281, 727
AclWI GGATC 2 cut(s) 374, 387
AfaI GTAC 2 cut(s) 5, 221
AfiI CCNNNNNNNGG 2 cut(s) 174, 343
AflIII ACRYGT 2 cut(s) 107, 477
AgsI TTSAA 4 cut(s) 37, 470, 632, 708
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 4 cut(s) 173, 620, 743, 841
AluI AGCT 4 cut(s) 173, 620, 743, 841
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 2 cut(s) 374, 387
ApeKI GCWGC 4 cut(s) 99, 235, 238, 838
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 420
AsuHPI GGTGA 3 cut(s) 241, 345, 661
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 211
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 2 cut(s) 185, 310
BauI CACGAG 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 111, 222, 225
BccI CCATC 1 cut(s) 789
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 68
BfuI GTATCC 1 cut(s) 175
BglII AGATCT 1 cut(s) 657
BisI GCNGC 5 cut(s) 75, 100, 236, 239, 839
BlsI GCNGC 5 cut(s) 76, 101, 237, 240, 840
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 4 cut(s) 187, 312, 381, 421
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 4 cut(s) 65, 222, 420, 832
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 2 cut(s) 321, 642
BpuEI CTTGAG 2 cut(s) 362, 534
BsaJI CCNNGG 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 343
Bse1I ACTGG 1 cut(s) 653
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 93, 780
BseLI CCNNNNNNNGG 2 cut(s) 174, 343
BseNI ACTGG 1 cut(s) 653
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 3 cut(s) 111, 222, 225
BseYI CCCAGC 1 cut(s) 173
BshNI GGYRCC 2 cut(s) 185, 310
BsiHKAI GWGCWC 1 cut(s) 159
BslFI GGGAC 3 cut(s) 488, 659, 774
BslI CCNNNNNNNGG 2 cut(s) 174, 343
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 3 cut(s) 488, 659, 774
Bsp1286I GDGCHC 2 cut(s) 159, 211
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 243, 379, 657
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 4 cut(s) 75, 227, 281, 727
BspLI GGNNCC 4 cut(s) 187, 312, 381, 421
BspMI ACCTGC 1 cut(s) 68
BspPI GGATC 2 cut(s) 374, 387
BspT107I GGYRCC 2 cut(s) 185, 310
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 653
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 3 cut(s) 243, 379, 657
BssSI CACGAG 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 22, 268, 492, 569
Bst6I CTCTTC 1 cut(s) 826
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 190, 506
BstDEI CTNAG 2 cut(s) 52, 435
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 93, 780
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 3 cut(s) 246, 382, 660
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 3 cut(s) 243, 379, 657
BstMWI GCNNNNNNNGC 2 cut(s) 542, 740
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 111, 481
BstSCI CCNGG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 3 cut(s) 111, 222, 225
BstX2I RGATCY 2 cut(s) 379, 657
BstXI CCANNNNNNTGG 1 cut(s) 679
BstYI RGATCY 2 cut(s) 379, 657
BsuI GTATCC 1 cut(s) 175
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 2 cut(s) 93, 780
BtsIMutI CAGTG 2 cut(s) 27, 273
BveI ACCTGC 1 cut(s) 68
Cac8I GCNNGC 2 cut(s) 190, 506
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 420
Csp6I GTAC 2 cut(s) 4, 220
CviAII CATG 9 cut(s) 108, 197, 357, 415, 478, 519, 532, 612, 679
CviJI RGCY 5 cut(s) 173, 508, 620, 743, 841
CviKI_1 RGCY 5 cut(s) 173, 508, 620, 743, 841
CviQI GTAC 2 cut(s) 4, 220
DdeI CTNAG 2 cut(s) 52, 435
DpnI GATC 3 cut(s) 245, 381, 659
DpnII GATC 3 cut(s) 243, 379, 657
Eam1104I CTCTTC 1 cut(s) 826
EarI CTCTTC 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 356
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 9 cut(s) 111, 200, 360, 418, 481, 522, 535, 615, 682
FaqI GGGAC 3 cut(s) 488, 659, 774
FatI CATG 9 cut(s) 107, 196, 356, 414, 477, 518, 531, 611, 678
FauNDI CATATG 1 cut(s) 760
Fnu4HI GCNGC 5 cut(s) 75, 100, 236, 239, 839
FokI GGATG 2 cut(s) 100, 787
Fsp4HI GCNGC 5 cut(s) 75, 100, 236, 239, 839
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 582
GluI GCNGC 5 cut(s) 75, 100, 236, 239, 839
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 321, 642
HhaI GCGC 1 cut(s) 583
Hin1II CATG 9 cut(s) 111, 200, 360, 418, 481, 522, 535, 615, 682
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HinfI GANTC 2 cut(s) 547, 608
HphI GGTGA 3 cut(s) 241, 345, 661
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 5 cut(s) 34, 467, 551, 629, 640
Hpy8I GTNNAC 1 cut(s) 220
HpyCH4III ACNGT 4 cut(s) 22, 268, 492, 569
HpyCH4V TGCA 3 cut(s) 411, 504, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 740
HpyF3I CTNAG 2 cut(s) 52, 435
Hsp92II CATG 9 cut(s) 111, 200, 360, 418, 481, 522, 535, 615, 682
HspAI GCGC 1 cut(s) 581
Kzo9I GATC 3 cut(s) 243, 379, 657
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 3 cut(s) 111, 222, 225
LweI GCATC 4 cut(s) 65, 222, 420, 832
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 14, 43, 283
MalI GATC 3 cut(s) 245, 381, 659
MboI GATC 3 cut(s) 243, 379, 657
MboII GAAGA 2 cut(s) 644, 843
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 2 cut(s) 379, 657
MhlI GDGCHC 2 cut(s) 159, 211
MluCI AATT 5 cut(s) 60, 385, 523, 586, 708
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 2 cut(s) 536, 551
MnlI CCTC 8 cut(s) 50, 131, 301, 337, 357, 519, 636, 677
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 677
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 2 cut(s) 542, 740
NcoI CCATGG 1 cut(s) 356
NdeI CATATG 1 cut(s) 760
NdeII GATC 3 cut(s) 243, 379, 657
NlaIII CATG 9 cut(s) 111, 200, 360, 418, 481, 522, 535, 615, 682
NlaIV GGNNCC 4 cut(s) 187, 312, 381, 421
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 2 cut(s) 111, 481
PciI ACATGT 2 cut(s) 107, 477
PfeI GAWTC 1 cut(s) 608
PkrI GCNGC 5 cut(s) 76, 101, 237, 240, 840
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PscI ACATGT 2 cut(s) 107, 477
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 187, 312, 381, 421
PspPI GGNCC 1 cut(s) 420
PsuI RGATCY 2 cut(s) 379, 657
RsaI GTAC 2 cut(s) 5, 221
RsaNI GTAC 2 cut(s) 4, 220
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 5 cut(s) 75, 100, 236, 239, 839
Sau3AI GATC 3 cut(s) 243, 379, 657
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 159, 211
SfaNI GCATC 4 cut(s) 65, 222, 420, 832
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 2 cut(s) 341, 549
SmoI CTYRAG 2 cut(s) 341, 549
Sse9I AATT 5 cut(s) 60, 385, 523, 586, 708
SsiI CCGC 4 cut(s) 75, 227, 281, 727
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 22, 268, 492, 569
TasI AATT 5 cut(s) 60, 385, 523, 586, 708
TatI WGTACW 2 cut(s) 3, 219
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 27, 273
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 4 cut(s) 99, 235, 238, 838
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 3 cut(s) 169, 507, 600
TspGWI ACGGA 2 cut(s) 764, 776
TspRI CASTG 2 cut(s) 27, 273
VpaK11BI GGWCC 1 cut(s) 420
XceI RCATGY 2 cut(s) 111, 481
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.