MD07G1176400.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009503
Physical Location & Seq
Reverse (-)
25571147 .. 25574282
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1176400.v1.1.491

Sequence Viewer

Length: 849 bp
ATGTACAGGTTCAGCAACACAGTGATTGGGTTCTTGAACCTCTTCAGTCTCTTAGCATCAATACCAATAATCGCCGGAGGTCTATGGATGGCAAGGAGCAGCACCACATGTGAAACTTTCCTCCAAACCCCACTTTTGGTGGTGGGTTTTGTGGTGCTCATAGTATCCCTAGCTGGGTTTATTGGTGCCTGCTTCCATGTGGCCTGGGCACTCTGGGTGTACTTGGTGGTGATGCTGCTGATTATTGCAACCCTAATGGGTTTGACAGTGTTTGGGTTTGTGGTTACAAGCCAAGGTGCTGGAGTGGAGGTGCCTGGTAGGGTTTATAAGGAGTACCACCTTGAGTATTACTCACCATGGTTAAGGAATAGGGTTAACGATCCTAATTATTGGAGTAAGATTAGGAGTTGCATCTTGGGGTCTAAAACTTGTGCTGAGCTTATTGCTTGGACACCTCTTGATTATCTTGAAAGAGACATGTCTCCAATACAGTCTGGTTGTTGCAAGCCCCCAACTTTGTGCAATTACAACATGGTAACTACAGTGAGTCAAGACCAAGATTGCTACCGGTGGAATAATGCGCCTAATATGTTATGTTACGAGTGTGATTCGTGCAAAGCTGGAGTTCTTGAAGATATCAAGAGGGACTGGCACAAGCTCTCTGTGTTGAACATTGTCGTGCTTGTGGTCCTAATTGGAGTATATTCAATCGGTTGCTGTGCTTTCCGAAACACACAACGAGCTGAAACAGATCACCCGTATGGTCAAAACCGGATGAGCAAAGTTCGTCCCCGATGGGATTACCACTGGTGGAGATGGTGGCACCACAGAAGAGAACAGCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

283

Amino Acids

32.51

Weight (kDa)

8.53

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 241 6.5e-35 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AccB1I GGYRCC 3 cut(s) 185, 310, 822
AclWI GGATC 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 28
AfaI GTAC 3 cut(s) 5, 221, 335
AfiI CCNNNNNNNGG 2 cut(s) 136, 174
AflIII ACRYGT 2 cut(s) 107, 477
AgeI ACCGGT 1 cut(s) 567
AgsI TTSAA 5 cut(s) 37, 470, 632, 670, 708
AjnI CCWGG 2 cut(s) 203, 313
AluBI AGCT 6 cut(s) 173, 439, 620, 658, 743, 841
AluI AGCT 6 cut(s) 173, 439, 620, 658, 743, 841
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 3 cut(s) 53, 468, 486
AlwI GGATC 1 cut(s) 374
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 99, 235
AsiGI ACCGGT 1 cut(s) 567
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 688
AsuHPI GGTGA 3 cut(s) 241, 345, 746
AvaII GGWCC 1 cut(s) 688
BaeGI GKGCMC 1 cut(s) 211
BanI GGYRCC 3 cut(s) 185, 310, 822
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 111, 222
BccI CCATC 3 cut(s) 82, 789, 810
BciT130I CCWGG 2 cut(s) 205, 315
BciVI GTATCC 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 53, 468, 486
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 175
BisI GCNGC 2 cut(s) 100, 236
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 2 cut(s) 101, 237
Bme1390I CCNGG 2 cut(s) 205, 315
Bme18I GGWCC 1 cut(s) 688
BmgT120I GGNCC 1 cut(s) 688
BmiI GGNNCC 3 cut(s) 187, 312, 824
BmrFI CCNGG 2 cut(s) 205, 315
BmsI GCATC 3 cut(s) 65, 222, 420
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 2 cut(s) 321, 642
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 362
BsaJI CCNNGG 3 cut(s) 204, 292, 356
BsaWI WCCGGW 2 cut(s) 567, 771
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 174
Bse118I RCCGGY 1 cut(s) 567
Bse1I ACTGG 2 cut(s) 653, 812
BseBI CCWGG 2 cut(s) 205, 315
BseDI CCNNGG 3 cut(s) 204, 292, 356
BseGI GGATG 2 cut(s) 93, 780
BseLI CCNNNNNNNGG 2 cut(s) 136, 174
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 653, 812
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 111, 222
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 3 cut(s) 185, 310, 822
BshTI ACCGGT 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 3 cut(s) 75, 568, 772
BslFI GGGAC 2 cut(s) 659, 774
BslI CCNNNNNNNGG 2 cut(s) 136, 174
BsmAI GTCTC 3 cut(s) 53, 468, 486
BsmFI GGGAC 2 cut(s) 659, 774
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 159, 211
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 379, 751
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 1 cut(s) 356
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 427
BspLI GGNNCC 3 cut(s) 187, 312, 824
BspPI GGATC 1 cut(s) 374
BspT107I GGYRCC 3 cut(s) 185, 310, 822
BsrFI RCCGGY 1 cut(s) 567
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 653, 812
BssAI RCCGGY 1 cut(s) 567
BssECI CCNNGG 3 cut(s) 204, 292, 356
BssMI GATC 2 cut(s) 379, 751
BssT1I CCWWGG 2 cut(s) 292, 356
Bst2UI CCWGG 2 cut(s) 205, 315
Bst4CI ACNGT 4 cut(s) 22, 268, 492, 544
Bst6I CTCTTC 2 cut(s) 47, 826
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 190, 506
BstDEI CTNAG 2 cut(s) 52, 435
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 93, 780
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 2 cut(s) 382, 754
BstMAI GTCTC 3 cut(s) 53, 468, 486
BstMBI GATC 2 cut(s) 379, 751
BstNI CCWGG 2 cut(s) 205, 315
BstNSI RCATGY 2 cut(s) 111, 481
BstSCI CCNGG 2 cut(s) 203, 313
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 111, 222
BstXI CCANNNNNNTGG 1 cut(s) 299
BsuI GTATCC 1 cut(s) 175
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 2 cut(s) 93, 780
BtsIMutI CAGTG 4 cut(s) 27, 273, 549, 805
Cac8I GCNNGC 2 cut(s) 190, 506
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 688
Csp6I GTAC 3 cut(s) 4, 220, 334
CspAI ACCGGT 1 cut(s) 567
CviAII CATG 5 cut(s) 108, 197, 357, 478, 532
CviJI RGCY 9 cut(s) 173, 203, 291, 439, 508, 620, 658, 743, 841
CviKI_1 RGCY 9 cut(s) 173, 203, 291, 439, 508, 620, 658, 743, 841
CviQI GTAC 3 cut(s) 4, 220, 334
DdeI CTNAG 2 cut(s) 52, 435
DpnI GATC 2 cut(s) 381, 753
DpnII GATC 2 cut(s) 379, 751
Eam1104I CTCTTC 2 cut(s) 47, 826
EarI CTCTTC 2 cut(s) 47, 826
Eco130I CCWWGG 2 cut(s) 292, 356
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 688
Eco57I CTGAAG 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 203, 313
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 292, 356
ErhI CCWWGG 2 cut(s) 292, 356
FaeI CATG 5 cut(s) 111, 200, 360, 481, 535
FaqI GGGAC 2 cut(s) 659, 774
FatI CATG 5 cut(s) 107, 196, 356, 477, 531
Fnu4HI GCNGC 2 cut(s) 100, 236
FokI GGATG 2 cut(s) 100, 787
Fsp4HI GCNGC 2 cut(s) 100, 236
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 582
GluI GCNGC 2 cut(s) 100, 236
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 321, 642
HaeIII GGCC 1 cut(s) 203
HapII CCGG 3 cut(s) 75, 568, 772
HhaI GCGC 1 cut(s) 583
Hin1II CATG 5 cut(s) 111, 200, 360, 481, 535
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 2 cut(s) 547, 608
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 3 cut(s) 75, 568, 772
HphI GGTGA 3 cut(s) 241, 345, 746
Hpy166II GTNNAC 2 cut(s) 220, 376
Hpy188I TCNGA 1 cut(s) 728
Hpy188III TCNNGA 6 cut(s) 34, 458, 467, 551, 629, 640
Hpy8I GTNNAC 2 cut(s) 220, 376
HpyCH4III ACNGT 4 cut(s) 22, 268, 492, 544
HpyCH4V TGCA 5 cut(s) 248, 411, 504, 522, 615
HpyF3I CTNAG 2 cut(s) 52, 435
Hsp92II CATG 5 cut(s) 111, 200, 360, 481, 535
HspAI GCGC 1 cut(s) 581
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 2 cut(s) 379, 751
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 111, 222
LweI GCATC 3 cut(s) 65, 222, 420
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 283, 535, 596
MalI GATC 2 cut(s) 381, 753
MboI GATC 2 cut(s) 379, 751
MboII GAAGA 3 cut(s) 34, 644, 843
MhlI GDGCHC 2 cut(s) 159, 211
MluCI AATT 3 cut(s) 385, 523, 693
MlyI GAGTC 1 cut(s) 556
MnlI CCTC 6 cut(s) 50, 71, 131, 301, 465, 636
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 2 cut(s) 362, 375
MslI CAYNNNNRTG 2 cut(s) 677, 759
MspI CCGG 3 cut(s) 75, 568, 772
MspR9I CCNGG 2 cut(s) 205, 315
MvaI CCWGG 2 cut(s) 205, 315
NcoI CCATGG 1 cut(s) 356
NdeII GATC 2 cut(s) 379, 751
NlaIII CATG 5 cut(s) 111, 200, 360, 481, 535
NlaIV GGNNCC 3 cut(s) 187, 312, 824
NspI RCATGY 2 cut(s) 111, 481
PciI ACATGT 2 cut(s) 107, 477
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 567
PkrI GCNGC 2 cut(s) 101, 237
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PscI ACATGT 2 cut(s) 107, 477
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 203, 313
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 203, 313
PspN4I GGNNCC 3 cut(s) 187, 312, 824
PspPI GGNCC 1 cut(s) 688
RsaI GTAC 3 cut(s) 5, 221, 335
RsaNI GTAC 3 cut(s) 4, 220, 334
RseI CAYNNNNRTG 2 cut(s) 677, 759
SaqAI TTAA 2 cut(s) 362, 375
SatI GCNGC 2 cut(s) 100, 236
Sau3AI GATC 2 cut(s) 379, 751
Sau96I GGNCC 1 cut(s) 688
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 2 cut(s) 205, 315
SduI GDGCHC 2 cut(s) 159, 211
SfaNI GCATC 3 cut(s) 65, 222, 420
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 677, 759
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 385, 523, 693
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 203, 313
StyI CCWWGG 2 cut(s) 292, 356
TaaI ACNGT 4 cut(s) 22, 268, 492, 544
TasI AATT 3 cut(s) 385, 523, 693
TatI WGTACW 2 cut(s) 3, 219
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 2 cut(s) 362, 375
Tru9I TTAA 2 cut(s) 362, 375
TscAI CASTG 4 cut(s) 27, 273, 549, 812
TseI GCWGC 2 cut(s) 99, 235
TspRI CASTG 4 cut(s) 27, 273, 549, 812
VpaK11BI GGWCC 1 cut(s) 688
XceI RCATGY 2 cut(s) 111, 481
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.