Rroxscaffold_4G00292960

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009503
Physical Location & Seq
Forward (+)
13213452 .. 13216159
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292960.1

Sequence Viewer

Length: 678 bp
ATGTATAGGTTGAGCAACACAGTGATTGGGTTCTTGAACCTCTTCACTCTTTTAGCATCAATACCTATTATTGGTGGTGGTCTATGGATGGCAAGGAGCAGCACAACATGTGAAAGCTTCCTCCAAACCCCACTCTTAGTGATTGGTTTTGTTGTGCTAATCATATCTCTGGCTGGGTTCATTGGTGCTTGTTTCCATGTCGCTTGGGCGCTTTGGGTGTACTTAGTAGTCATGCTGTTCCTTATTGCAACCCTGATGGGGTTGACTGTGTTTGGGTTTGTGGTTACAAGTCAAGGTGCTGGAGTTGATGTGCCTGGTAGGGCATATAAGGAATACCATCTCGAGGATTACTCACCATGGTTGAGGAAGAGGATTAAGGACCCCAATTACTGGAGCAACATTAGGAGTTGTATTTTGGGGTCTAAGACTTGTGCAAAGCTTCTTCTTTGGACTCCTCTCGATTATCTTGAGAGGGATATGTCTCCAATACAGTCTGGTTGTTGCAAACCACCAACAGCATGCAACTACAACATGGCTACAACAGTGAGTCAAGACCCAGATTGCTACCGGTGGAACAATGCCCCGAATTTGTTATGCTACGAGTGTGATTCATGCAAGGCAGGAGTGCTTGAAAATATCAAAGGGACTGGCACAAGCTCTCTGTTCTCAACATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

225

Amino Acids

24.93

Weight (kDa)

7.98

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 216 3.2e-33 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 390
AcsI RAATTY 1 cut(s) 586
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 4 cut(s) 71, 258, 343, 390
AflIII ACRYGT 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 567
AgsI TTSAA 2 cut(s) 37, 632
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 117, 439, 657
AluI AGCT 3 cut(s) 117, 439, 657
Alw26I GTCTC 1 cut(s) 486
Ama87I CYCGRG 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 586
AsiGI ACCGGT 1 cut(s) 567
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 345
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 111
BccI CCATC 3 cut(s) 82, 250, 345
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 379
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 65
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 2 cut(s) 321, 412
BpuEI CTTGAG 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 356
BsaWI WCCGGW 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 258, 343, 390
Bse118I RCCGGY 1 cut(s) 567
Bse1I ACTGG 2 cut(s) 395, 652
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 71, 258, 343, 390
BseNI ACTGG 2 cut(s) 395, 652
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 568
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 4 cut(s) 71, 258, 343, 390
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 658
BsoBI CYCGRG 1 cut(s) 341
Bsp19I CCATGG 1 cut(s) 356
BspLI GGNNCC 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 567
BsrI ACTGG 2 cut(s) 395, 652
BssAI RCCGGY 1 cut(s) 567
BssECI CCNNGG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 22, 268, 492, 544
Bst6I CTCTTC 2 cut(s) 47, 362
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 3 cut(s) 136, 223, 423
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 486
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 111, 522
BstSCI CCNGG 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 111
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 27, 549
Cac8I GCNNGC 1 cut(s) 520
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 220
CspAI ACCGGT 1 cut(s) 567
CviAII CATG 7 cut(s) 108, 197, 232, 357, 519, 532, 612
CviJI RGCY 5 cut(s) 117, 173, 439, 536, 657
CviKI_1 RGCY 5 cut(s) 117, 173, 439, 536, 657
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 136, 223, 423
Eam1104I CTCTTC 2 cut(s) 47, 362
EarI CTCTTC 2 cut(s) 47, 362
Eco130I CCWWGG 1 cut(s) 356
Eco47I GGWCC 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 7 cut(s) 111, 200, 235, 360, 522, 535, 615
FaqI GGGAC 1 cut(s) 658
FatI CATG 7 cut(s) 107, 196, 231, 356, 518, 531, 611
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 100
GlaI GCGC 1 cut(s) 210
GluI GCNGC 1 cut(s) 100
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 321, 412
HaeII RGCGCY 1 cut(s) 212
HapII CCGG 1 cut(s) 568
HhaI GCGC 1 cut(s) 211
Hin1II CATG 7 cut(s) 111, 200, 235, 360, 522, 535, 615
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HindIII AAGCTT 2 cut(s) 115, 437
HinfI GANTC 3 cut(s) 451, 547, 608
HpaII CCGG 1 cut(s) 568
HphI GGTGA 1 cut(s) 345
Hpy166II GTNNAC 2 cut(s) 220, 264
Hpy188III TCNNGA 5 cut(s) 34, 341, 458, 467, 551
Hpy8I GTNNAC 2 cut(s) 220, 264
HpyCH4III ACNGT 4 cut(s) 22, 268, 492, 544
HpyCH4V TGCA 5 cut(s) 248, 434, 504, 522, 615
HpyF3I CTNAG 3 cut(s) 136, 223, 423
Hsp92II CATG 7 cut(s) 111, 200, 235, 360, 522, 535, 615
HspAI GCGC 1 cut(s) 209
LmnI GCTCC 2 cut(s) 96, 393
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 3 cut(s) 34, 379, 434
MluCI AATT 2 cut(s) 385, 586
MlyI GAGTC 2 cut(s) 445, 556
MnlI CCTC 7 cut(s) 50, 131, 337, 357, 363, 465, 465
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 1 cut(s) 375
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 356
NlaIII CATG 7 cut(s) 111, 200, 235, 360, 522, 535, 615
NlaIV GGNNCC 1 cut(s) 381
NspI RCATGY 2 cut(s) 111, 522
PaeI GCATGC 1 cut(s) 522
PaeR7I CTCGAG 1 cut(s) 341
PciI ACATGT 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 608
PflMI CCANNNNNTGG 1 cut(s) 390
PinAI ACCGGT 1 cut(s) 567
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 2 cut(s) 445, 555
PpsI GAGTC 2 cut(s) 445, 555
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 100
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 2 cut(s) 445, 556
ScrFI CCNGG 1 cut(s) 315
SetI ASST 7 cut(s) 11, 42, 67, 119, 298, 441, 659
SfaNI GCATC 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 341
SinI GGWCC 1 cut(s) 379
SlaI CTCGAG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 341, 467
SmoI CTYRAG 2 cut(s) 341, 467
SphI GCATGC 1 cut(s) 522
Sse9I AATT 2 cut(s) 385, 586
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 22, 268, 492, 544
TaqI TCGA 2 cut(s) 342, 459
TasI AATT 2 cut(s) 385, 586
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 27, 549
TseI GCWGC 1 cut(s) 99
TspDTI ATGAA 2 cut(s) 169, 600
TspRI CASTG 2 cut(s) 27, 549
Van91I CCANNNNNTGG 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 1 cut(s) 586
XceI RCATGY 2 cut(s) 111, 522
XhoI CTCGAG 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.