FvH4_7g22001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002480
Physical Location & Seq
Forward (+)
17476798 .. 17480304
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22001.t1

Sequence Viewer

Length: 1194 bp
ATGCGAAGATCGATCGCGAGCCTGGGCGGGCCCCTCAGCCGAGGGTTCAGCACGACTTCGTCGTCGGAGAAAATCGTCGCCTCCGTACTCTTCGAGAGGCTCCCGGTTGTTATCCCCAGAATCGACCCCGTCGTCTATGCATTTCAGGAGTTCTCGTTTCGGTGGCGACAGCAATATCGACGGAGATATCCGGATGAATTGTTAGACAAGTCTAATGCTAGTTTATCAAGCACTTTTCCGGCTGTAGCCTTCTTTTATTTCCCTTTCTGGATGCTGGACTGTCCTGAGCTGGATTATGATTATCTGGCATCCCCAACAACGATGGACTTATCTTACAGTGTGATTTTAGAACATACTATTCCTGAGAGGGAGCACAGTCCTGCAGTTGTAGCAAGGTGTGTGCCACTTTTGAAACGATACCTTTTAAGGTACAAACCCAGCGAAGAGACATTACTGCAGATTGATCGGTTTTGTGTAAACACTATTGCTGAATGTGATATTGGTCCAAACCGGAAATTGTCTCCATGGTCACAGTCGGTTGCGTCAACAGCATCTACAAATACTCTCCCTTTGTCTGTACCTAGATTTGCTTCTGGGACTTGTGTGAAGTCATTGAACTATGTGCGCTCTCTGGTGTCTCGACATCTTCCAAGGCGATCATTCCACCCGGGTGCTTTTTCTGGAGCTCTGTCTGCAACAAGACAATCCCTTCCGAGTTTGTCATCTTTGTTGAGTAGATCCTTCAATGGGCAGCTAAGCCCTGCACGCAGTGGGGAATCTTCGGAGAATAAAGATGTCACAACTATGTCTATTTTGAACCTATCAAATATTGAAAAGGTTGATGGAATGAAAGATCTTGAATGCTTTGCACTGGACGTTCTCAGGTGGCGCTGGCTTGGAGAACAGCGGTCATCATTATTGCTAACTGAAAGTGATCGCGTTGCAAATTCCCAAGAAATGAGAACATATAATCTCCTAGAAGTAGGTGTAGCAGCTCTACTTGTAGGAGATTTGAAAGCTAAAATGAAAGGTCAACCTTGGAAGTTTTTCGGAACAGCTGATATGCCTTATCTTGATCAACTGTTGCAACCTTCACCAGTATCCGCAATTACTGATTCTTCTGCGGCACATGCCCACCTGATGGCAATAACAGCATGTAAACGCACTAAATCCAGCCCTAGTCAAATATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

398

Amino Acids

44.39

Weight (kDa)

9.05

Isoelectric Point (pI)

69.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 61, 131
AccB7I CCANNNNNTGG 1 cut(s) 1141
AccII CGCG 2 cut(s) 17, 939
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 4 cut(s) 27, 907, 1104, 1124
AclWI GGATC 1 cut(s) 732
AcsI RAATTY 1 cut(s) 946
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 3 cut(s) 87, 431, 579
AfiI CCNNNNNNNGG 2 cut(s) 747, 1141
AgsI TTSAA 7 cut(s) 412, 616, 745, 817, 833, 860, 1015
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AleI CACNNNNGTG 1 cut(s) 669
AluBI AGCT 6 cut(s) 289, 686, 754, 995, 1019, 1058
AluI AGCT 6 cut(s) 289, 686, 754, 995, 1019, 1058
Alw21I GWGCWC 2 cut(s) 375, 688
Alw26I GTCTC 3 cut(s) 440, 525, 642
AlwI GGATC 1 cut(s) 732
Ama87I CYCGRG 1 cut(s) 667
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 1 cut(s) 29
ApaI GGGCCC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 751, 992
ApoI RAATTY 1 cut(s) 946
Asp700I GAANNNNTTC 1 cut(s) 1046
AspLEI GCGC 2 cut(s) 627, 891
AspS9I GGNCC 3 cut(s) 29, 30, 503
AsuC2I CCSGG 3 cut(s) 104, 668, 669
AsuHPI GGTGA 1 cut(s) 1086
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 2 cut(s) 33, 688
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
Bbv12I GWGCWC 2 cut(s) 375, 688
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 763, 1004
BccI CCATC 3 cut(s) 316, 836, 1135
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 1 cut(s) 1111
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 3 cut(s) 104, 668, 669
BcoDI GTCTC 3 cut(s) 440, 525, 642
BfaI CTAG 5 cut(s) 219, 582, 977, 1179, 1192
BfmI CTRYAG 3 cut(s) 243, 381, 455
BfoI RGCGCY 1 cut(s) 892
BfuI GTATCC 1 cut(s) 1111
BglII AGATCT 1 cut(s) 853
BisI GCNGC 3 cut(s) 752, 993, 1125
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 3 cut(s) 753, 994, 1126
Bme1390I CCNGG 4 cut(s) 23, 104, 668, 669
Bme18I GGWCC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 3 cut(s) 29, 30, 503
BmiI GGNNCC 3 cut(s) 31, 32, 101
BmrFI CCNGG 4 cut(s) 23, 104, 668, 669
BmsI GCATC 3 cut(s) 261, 317, 560
BpmI CTGGAG 1 cut(s) 702
Bpu10I CCTNAGC 2 cut(s) 35, 285
Bpu1102I GCTNAGC 1 cut(s) 755
BpuMI CCSGG 3 cut(s) 104, 668, 669
Bsa29I ATCGAT 1 cut(s) 11
BsaJI CCNNGG 6 cut(s) 22, 40, 524, 650, 667, 1037
BsaWI WCCGGW 2 cut(s) 190, 510
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 747, 1141
Bse1I ACTGG 2 cut(s) 876, 1097
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 6 cut(s) 22, 40, 524, 650, 667, 1037
BseGI GGATG 3 cut(s) 199, 276, 308
BseLI CCNNNNNNNGG 2 cut(s) 747, 1141
BseMII CTCAG 4 cut(s) 49, 276, 354, 895
BseNI ACTGG 2 cut(s) 876, 1097
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 2 cut(s) 763, 1004
BseYI CCCAGC 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 2 cut(s) 17, 939
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 375, 688
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 5 cut(s) 104, 191, 239, 511, 668
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 2 cut(s) 747, 1141
BsmAI GTCTC 3 cut(s) 440, 525, 642
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 1 cut(s) 31
BsoBI CYCGRG 1 cut(s) 667
Bsp120I GGGCCC 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 33, 375, 688
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
Bsp1720I GCTNAGC 1 cut(s) 755
Bsp19I CCATGG 1 cut(s) 524
Bsp68I TCGCGA 1 cut(s) 17
BspACI CCGC 4 cut(s) 27, 907, 1104, 1124
BspANI GGCC 1 cut(s) 31
BspCNI CTCAG 4 cut(s) 48, 277, 355, 894
BspDI ATCGAT 1 cut(s) 11
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 2 cut(s) 17, 939
BspLI GGNNCC 3 cut(s) 31, 32, 101
BspMAI CTGCAG 2 cut(s) 385, 459
BspPI GGATC 1 cut(s) 732
BsrI ACTGG 2 cut(s) 876, 1097
BssECI CCNNGG 6 cut(s) 22, 40, 524, 650, 667, 1037
BssMI GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
BssT1I CCWWGG 3 cut(s) 524, 650, 1037
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 5 cut(s) 281, 338, 377, 534, 1083
Bst6I CTCTTC 2 cut(s) 95, 438
BstC8I GCNNGC 4 cut(s) 19, 29, 766, 893
BstDEI CTNAG 5 cut(s) 35, 285, 363, 755, 881
BstDSI CCRYGG 1 cut(s) 524
BstF5I GGATG 3 cut(s) 199, 276, 308
BstFNI CGCG 2 cut(s) 17, 939
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 2 cut(s) 627, 891
BstKTI GATC 8 cut(s) 11, 15, 466, 659, 740, 856, 937, 1078
BstMAI GTCTC 3 cut(s) 440, 525, 642
BstMBI GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 6 cut(s) 389, 548, 692, 765, 1130, 1151
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 1133, 1158
BstSCI CCNGG 4 cut(s) 21, 102, 666, 667
BstSFI CTRYAG 3 cut(s) 243, 381, 455
BstSLI GKGCMC 1 cut(s) 33
BstUI CGCG 2 cut(s) 17, 939
BstV1I GCAGC 2 cut(s) 763, 1004
BstX2I RGATCY 2 cut(s) 737, 853
BstYI RGATCY 2 cut(s) 737, 853
Bsu15I ATCGAT 1 cut(s) 11
BsuI GTATCC 1 cut(s) 1111
BsuRI GGCC 1 cut(s) 31
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 524
BtsCI GGATG 3 cut(s) 199, 276, 308
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 3 cut(s) 343, 775, 869
BtuMI TCGCGA 1 cut(s) 17
Cac8I GCNNGC 4 cut(s) 19, 29, 766, 893
CfoI GCGC 2 cut(s) 627, 891
Cfr13I GGNCC 3 cut(s) 29, 30, 503
Cfr9I CCCGGG 1 cut(s) 667
ClaI ATCGAT 1 cut(s) 11
CseI GACGC 1 cut(s) 531
Csp6I GTAC 3 cut(s) 86, 430, 578
CviAII CATG 3 cut(s) 525, 1130, 1155
CviQI GTAC 3 cut(s) 86, 430, 578
DdeI CTNAG 5 cut(s) 35, 285, 363, 755, 881
DpnI GATC 8 cut(s) 10, 14, 465, 658, 739, 855, 936, 1077
DpnII GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
DraIII CACNNNGTG 1 cut(s) 770
DrdI GACNNNNNNGTC 2 cut(s) 61, 131
DseDI GACNNNNNNGTC 2 cut(s) 61, 131
Eam1104I CTCTTC 2 cut(s) 95, 438
EarI CTCTTC 2 cut(s) 95, 438
Ecl136II GAGCTC 1 cut(s) 686
Eco130I CCWWGG 3 cut(s) 524, 650, 1037
Eco24I GRGCYC 2 cut(s) 33, 688
Eco32I GATATC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 503
Eco53kI GAGCTC 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 21
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 3 cut(s) 524, 650, 1037
EcoT22I ATGCAT 1 cut(s) 142
EcoT38I GRGCYC 2 cut(s) 33, 688
ErhI CCWWGG 3 cut(s) 524, 650, 1037
FaeI CATG 3 cut(s) 528, 1133, 1158
FaqI GGGAC 1 cut(s) 610
FatI CATG 3 cut(s) 524, 1129, 1154
FauI CCCGC 1 cut(s) 20
FbaI TGATCA 1 cut(s) 1075
Fnu4HI GCNGC 3 cut(s) 752, 993, 1125
FokI GGATG 3 cut(s) 206, 283, 295
FriOI GRGCYC 2 cut(s) 33, 688
Fsp4HI GCNGC 3 cut(s) 752, 993, 1125
FspBI CTAG 5 cut(s) 219, 582, 977, 1179, 1192
GlaI GCGC 2 cut(s) 626, 890
GluI GCNGC 3 cut(s) 752, 993, 1125
GsaI CCCAGC 1 cut(s) 441
GsuI CTGGAG 1 cut(s) 702
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 1 cut(s) 31
HapII CCGG 5 cut(s) 104, 191, 239, 511, 668
HgaI GACGC 1 cut(s) 531
HhaI GCGC 2 cut(s) 627, 891
Hin1II CATG 3 cut(s) 528, 1133, 1158
Hin6I GCGC 2 cut(s) 625, 889
HinP1I GCGC 2 cut(s) 625, 889
HincII GTYRAC 2 cut(s) 546, 1034
HindII GTYRAC 2 cut(s) 546, 1034
HinfI GANTC 3 cut(s) 120, 776, 1115
HpaII CCGG 5 cut(s) 104, 191, 239, 511, 668
HphI GGTGA 1 cut(s) 1086
Hpy166II GTNNAC 4 cut(s) 478, 546, 1034, 1160
Hpy188I TCNGA 4 cut(s) 67, 714, 784, 1052
Hpy8I GTNNAC 4 cut(s) 478, 546, 1034, 1160
Hpy99I CGWCG 5 cut(s) 64, 67, 80, 134, 183
HpyAV CCTTC 4 cut(s) 259, 719, 751, 1101
HpyCH4III ACNGT 5 cut(s) 281, 338, 377, 534, 1083
HpyCH4IV ACGT 1 cut(s) 876
HpyCH4V TGCA 8 cut(s) 140, 383, 457, 695, 764, 869, 944, 1087
HpyF10VI GCNNNNNNNGC 6 cut(s) 389, 548, 692, 765, 1130, 1151
HpyF3I CTNAG 5 cut(s) 35, 285, 363, 755, 881
HpySE526I ACGT 1 cut(s) 876
Hsp92II CATG 3 cut(s) 528, 1133, 1158
HspAI GCGC 2 cut(s) 625, 889
Kpn2I TCCGGA 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
LmnI GCTCC 3 cut(s) 105, 370, 683
Lsp1109I GCAGC 2 cut(s) 763, 1004
LweI GCATC 3 cut(s) 261, 317, 560
MaeI CTAG 5 cut(s) 219, 582, 977, 1179, 1192
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 2 cut(s) 528, 796
MalI GATC 8 cut(s) 10, 14, 465, 658, 739, 855, 936, 1077
MboI GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
MboII GAAGA 6 cut(s) 18, 82, 455, 638, 771, 1110
MflI RGATCY 2 cut(s) 737, 853
MhlI GDGCHC 3 cut(s) 33, 375, 688
MluCI AATT 4 cut(s) 197, 515, 946, 1107
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 5 cut(s) 35, 44, 90, 91, 360
Mph1103I ATGCAT 1 cut(s) 142
MroI TCCGGA 1 cut(s) 190
MroXI GAANNNNTTC 1 cut(s) 1046
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 2 cut(s) 669, 803
MspA1I CMGCKG 2 cut(s) 907, 1058
MspI CCGG 5 cut(s) 104, 191, 239, 511, 668
MspR9I CCNGG 4 cut(s) 23, 104, 668, 669
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 17, 939
MwoI GCNNNNNNNGC 6 cut(s) 389, 548, 692, 765, 1130, 1151
NciI CCSGG 3 cut(s) 104, 668, 669
NcoI CCATGG 1 cut(s) 524
NdeII GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
NlaIII CATG 3 cut(s) 528, 1133, 1158
NlaIV GGNNCC 3 cut(s) 31, 32, 101
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 528, 796
NruI TCGCGA 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 2 cut(s) 1133, 1158
OliI CACNNNNGTG 1 cut(s) 669
PcsI WCGNNNNNNNCGW 3 cut(s) 59, 81, 129
PctI GAATGC 1 cut(s) 866
PdmI GAANNNNTTC 1 cut(s) 1046
PfeI GAWTC 3 cut(s) 120, 776, 1115
PflFI GACNNNGTC 2 cut(s) 58, 128
PflMI CCANNNNNTGG 1 cut(s) 1141
PkrI GCNGC 3 cut(s) 753, 994, 1126
Ple19I CGATCG 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 688
Psp6I CCWGG 1 cut(s) 21
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 31, 32, 101
PspOMI GGGCCC 1 cut(s) 29
PspPI GGNCC 3 cut(s) 29, 30, 503
PstI CTGCAG 2 cut(s) 385, 459
PsuI RGATCY 2 cut(s) 737, 853
PsyI GACNNNGTC 2 cut(s) 58, 128
PvuI CGATCG 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 1058
RruI TCGCGA 1 cut(s) 17
RsaI GTAC 3 cut(s) 87, 431, 579
RsaNI GTAC 3 cut(s) 86, 430, 578
RseI CAYNNNNRTG 2 cut(s) 669, 803
SacI GAGCTC 1 cut(s) 688
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 3 cut(s) 752, 993, 1125
Sau3AI GATC 8 cut(s) 8, 12, 463, 656, 737, 853, 934, 1075
Sau96I GGNCC 3 cut(s) 29, 30, 503
ScrFI CCNGG 4 cut(s) 23, 104, 668, 669
SduI GDGCHC 3 cut(s) 33, 375, 688
SfaNI GCATC 3 cut(s) 261, 317, 560
SfcI CTRYAG 3 cut(s) 243, 381, 455
SinI GGWCC 1 cut(s) 503
SmaI CCCGGG 1 cut(s) 669
SmiMI CAYNNNNRTG 2 cut(s) 669, 803
Sse9I AATT 4 cut(s) 197, 515, 946, 1107
SsiI CCGC 4 cut(s) 27, 907, 1104, 1124
SspI AATATT 1 cut(s) 829
SspMI CTAG 5 cut(s) 219, 582, 977, 1179, 1192
SstI GAGCTC 1 cut(s) 688
StyD4I CCNGG 4 cut(s) 21, 102, 666, 667
StyI CCWWGG 3 cut(s) 524, 650, 1037
TaaI ACNGT 5 cut(s) 281, 338, 377, 534, 1083
TaiI ACGT 1 cut(s) 879
TaqI TCGA 5 cut(s) 11, 93, 123, 178, 640
TasI AATT 4 cut(s) 197, 515, 946, 1107
TauI GCSGC 1 cut(s) 1127
TfiI GAWTC 3 cut(s) 120, 776, 1115
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 3 cut(s) 343, 775, 876
TseFI GTSAC 2 cut(s) 528, 796
TseI GCWGC 2 cut(s) 751, 992
Tsp45I GTSAC 2 cut(s) 528, 796
TspDTI ATGAA 3 cut(s) 210, 863, 1040
TspGWI ACGGA 2 cut(s) 73, 196
TspMI CCCGGG 1 cut(s) 667
TspRI CASTG 3 cut(s) 343, 775, 876
Tth111I GACNNNGTC 2 cut(s) 58, 128
Van91I CCANNNNNTGG 1 cut(s) 1141
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 946
XceI RCATGY 2 cut(s) 1133, 1158
XmaI CCCGGG 1 cut(s) 667
XmnI GAANNNNTTC 1 cut(s) 1046
XspI CTAG 5 cut(s) 219, 582, 977, 1179, 1192
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.