Rmu_co8229963.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8229963.1
OG0002480
Physical Location & Seq
Forward (+)
1 .. 447
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8229963.1_g000001.1.cds

Sequence Viewer

Length: 447 bp
cggaaattgtctccattgtcacaatcatttgcgtcaacagcatctacaaatactccccctttgtctgtacctagtcctgggactcttgtgaagtcattaaactatgtgcggtctctggtgtctcaacatcttccaaggcgatcattccacccgggtgctttttctggagccctgtctgcaacaagacaatcccttccaagtttgtcttctttgttgagtagatccttcaatgggcagctaagccctgcacacactggggaacctttggagaataaagacgtcacaactatgtctattttgaacctatcaaatattgaaaaggttgatggaatgaaagatcttgaatacctcgcactcgacgttctcaggtggcgctggcttggagaacagcagtcatcattattgctaactgaaaggtacacacagaaatggcttgttaccttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.39

Weight (kDa)

9.89

Isoelectric Point (pI)

73.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AciI CCGC 1 cut(s) 109
AclWI GGATC 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 69, 419
AfiI CCNNNNNNNGG 2 cut(s) 77, 231
AgsI TTSAA 4 cut(s) 229, 301, 317, 344
AjnI CCWGG 1 cut(s) 76
AjuI GAANNNNNNNTTGG 2 cut(s) 190, 222
AleI CACNNNNGTG 1 cut(s) 153
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw26I GTCTC 3 cut(s) 15, 117, 126
AlwI GGATC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 235
AspLEI GCGC 1 cut(s) 375
AsuC2I CCSGG 2 cut(s) 152, 153
AvaI CYCGRG 1 cut(s) 151
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 247
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 78
BcnI CCSGG 2 cut(s) 152, 153
BcoDI GTCTC 3 cut(s) 15, 117, 126
BfaI CTAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 376
BglII AGATCT 1 cut(s) 337
BisI GCNGC 1 cut(s) 236
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 237
Bme1390I CCNGG 3 cut(s) 78, 152, 153
BmeT110I CYCGRG 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 169, 261
BmrFI CCNGG 3 cut(s) 78, 152, 153
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 50
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 186
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 152, 153
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 77, 134, 151
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 231
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 77, 134, 151
BseLI CCNNNNNNNGG 2 cut(s) 77, 231
BseMII CTCAG 1 cut(s) 379
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 247
BsgI GTGCAG 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 2 cut(s) 77, 231
BsmAI GTCTC 3 cut(s) 15, 117, 126
BsmFI GGGAC 1 cut(s) 94
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 140, 221, 337
Bsp1720I GCTNAGC 1 cut(s) 239
BspACI CCGC 1 cut(s) 109
BspCNI CTCAG 1 cut(s) 378
BspLI GGNNCC 2 cut(s) 169, 261
BspPI GGATC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 117
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 77, 134, 151
BssMI GATC 3 cut(s) 140, 221, 337
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 239, 365
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 3 cut(s) 143, 224, 340
BstMAI GTCTC 3 cut(s) 15, 117, 126
BstMBI GATC 3 cut(s) 140, 221, 337
BstMWI GCNNNNNNNGC 2 cut(s) 38, 176
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 3 cut(s) 76, 150, 151
BstV1I GCAGC 1 cut(s) 247
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 221, 337
BstYI RGATCY 2 cut(s) 221, 337
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 377
CfoI GCGC 1 cut(s) 375
Cfr9I CCCGGG 1 cut(s) 151
CseI GACGC 1 cut(s) 21
Csp6I GTAC 2 cut(s) 68, 418
CviJI RGCY 5 cut(s) 170, 238, 243, 379, 433
CviKI_1 RGCY 5 cut(s) 170, 238, 243, 379, 433
CviQI GTAC 2 cut(s) 68, 418
DdeI CTNAG 2 cut(s) 239, 365
DpnI GATC 3 cut(s) 142, 223, 339
DpnII GATC 3 cut(s) 140, 221, 337
Eco130I CCWWGG 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 134
FaiI YATR 2 cut(s) 105, 290
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FaqI GGGAC 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 1 cut(s) 236
FspBI CTAG 1 cut(s) 72
GlaI GCGC 1 cut(s) 374
GluI GCNGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 376
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 21
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 1 cut(s) 82
HpaII CCGG 1 cut(s) 152
Hpy166II GTNNAC 2 cut(s) 36, 420
Hpy188III TCNNGA 2 cut(s) 165, 341
Hpy8I GTNNAC 2 cut(s) 36, 420
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 203, 235
HpyCH4IV ACGT 2 cut(s) 279, 360
HpyCH4V TGCA 2 cut(s) 179, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 176
HpyF3I CTNAG 2 cut(s) 239, 365
HpySE526I ACGT 2 cut(s) 279, 360
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 1 cut(s) 373
Kzo9I GATC 3 cut(s) 140, 221, 337
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 1 cut(s) 50
MaeI CTAG 1 cut(s) 72
MaeII ACGT 2 cut(s) 279, 360
MaeIII GTNAC 3 cut(s) 18, 280, 436
MalI GATC 3 cut(s) 142, 223, 339
MboI GATC 3 cut(s) 140, 221, 337
MboII GAAGA 2 cut(s) 122, 198
MflI RGATCY 2 cut(s) 221, 337
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 5
MlyI GAGTC 1 cut(s) 76
MnlI CCTC 1 cut(s) 359
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 3 cut(s) 153, 287, 427
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 3 cut(s) 78, 152, 153
MvaI CCWGG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 38, 176
NciI CCSGG 2 cut(s) 152, 153
NdeII GATC 3 cut(s) 140, 221, 337
NlaIV GGNNCC 2 cut(s) 169, 261
NmuCI GTSAC 2 cut(s) 18, 280
OliI CACNNNNGTG 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PkrI GCNGC 1 cut(s) 237
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 169, 261
PsuI RGATCY 2 cut(s) 221, 337
RsaI GTAC 2 cut(s) 69, 419
RsaNI GTAC 2 cut(s) 68, 418
RseI CAYNNNNRTG 3 cut(s) 153, 287, 427
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 3 cut(s) 140, 221, 337
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 78, 152, 153
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 1 cut(s) 50
SmaI CCCGGG 1 cut(s) 153
SmiMI CAYNNNNRTG 3 cut(s) 153, 287, 427
Sse9I AATT 1 cut(s) 5
SsiI CCGC 1 cut(s) 109
SspI AATATT 1 cut(s) 313
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 76, 150, 151
StyI CCWWGG 1 cut(s) 134
TaiI ACGT 2 cut(s) 282, 363
TaqI TCGA 1 cut(s) 357
TasI AATT 1 cut(s) 5
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 2 cut(s) 18, 280
TseI GCWGC 1 cut(s) 235
Tsp45I GTSAC 2 cut(s) 18, 280
TspDTI ATGAA 1 cut(s) 347
TspMI CCCGGG 1 cut(s) 151
TspRI CASTG 1 cut(s) 259
XmaI CCCGGG 1 cut(s) 151
XspI CTAG 1 cut(s) 72
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.