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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G12590 AT3G12590 |
| fragaria_vesca | FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g21970 FvH4_7g22001 FvH4_7g22001 |
| malus_domestica | MD01G1124000.v1.1 MD07G1194200.v1.1 |
| prunus_persica | Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 Prupe.2G231600_v2.0.a1 |
| pyrus_communis | pycom01g15050 pycom07g18300 |
| rosa_chinensis | RchiOBHm_Chr1g0366301 |
| rosa_laevigata | RLG00000027354 |
| rosa_multiflora | Rmu_co8229963.1_g000001 Rmu_co8389475.1_g000001 Rmu_ssc0000250.1_g000001 |
| rosa_roxburghii | Rroxscaffold_4G00290010 Rroxscaffold_4G00290260 |
| rosa_rugosa | Rorug01G0328600 Rorug01G0328600 Rorug01G0328700.1 |
| rosa_samantha | Rh1AG336900 Rh1BG298500 Rh1CG313800 Rh1DG329900 |
| rosa_wichuraiana | Rw1G029870 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 729 |
| Acc36I | ACCTGC | 1 cut(s) | 2592 |
| AccI | GTMKAC | 1 cut(s) | 12 |
| AccII | CGCG | 2 cut(s) | 894, 3406 |
| AclWI | GGATC | 1 cut(s) | 663 |
| AcoI | YGGCCR | 4 cut(s) | 54, 162, 3154, 3275 |
| AcsI | RAATTY | 8 cut(s) | 219, 901, 1870, 2290, 2366, 2614, 2655, 2814 |
| AcuI | CTGAAG | 9 cut(s) | 32, 1424, 1959, 2253, 2676, 2688, 3431, 3494, 3512 |
| AcvI | CACGTG | 2 cut(s) | 1085, 3251 |
| AcyI | GRCGYC | 2 cut(s) | 152, 726 |
| AfaI | GTAC | 7 cut(s) | 362, 510, 865, 1935, 1966, 2358, 3434 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 136, 678, 1095, 1096 |
| AflIII | ACRYGT | 6 cut(s) | 871, 1389, 1469, 1920, 3248, 3250 |
| AhlI | ACTAGT | 2 cut(s) | 1852, 3140 |
| AjnI | CCWGG | 2 cut(s) | 3117, 3156 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 414, 446, 3429, 3461 |
| AleI | CACNNNNGTG | 2 cut(s) | 600, 1209 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2393, 2425 |
| Alw21I | GWGCWC | 2 cut(s) | 1166, 3469 |
| Alw26I | GTCTC | 9 cut(s) | 371, 456, 564, 573, 957, 1946, 1963, 2948, 3179 |
| AlwI | GGATC | 1 cut(s) | 663 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1637 |
| Ama87I | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| AoxI | GGCC | 7 cut(s) | 54, 162, 1129, 2511, 3154, 3275, 3362 |
| ApoI | RAATTY | 8 cut(s) | 219, 901, 1870, 2290, 2366, 2614, 2655, 2814 |
| AseI | ATTAAT | 2 cut(s) | 2142, 2949 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1887, 2796 |
| AspLEI | GCGC | 5 cut(s) | 129, 306, 822, 1230, 1849 |
| AspS9I | GGNCC | 4 cut(s) | 434, 1130, 2511, 3362 |
| AsuHPI | GGTGA | 3 cut(s) | 1041, 1278, 2275 |
| AvaI | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| AvaII | GGWCC | 1 cut(s) | 434 |
| AxyI | CCTNAGG | 1 cut(s) | 356 |
| BaeGI | GKGCMC | 1 cut(s) | 1090 |
| BalI | TGGCCA | 2 cut(s) | 3156, 3277 |
| BanII | GRGCYC | 3 cut(s) | 32, 619, 3469 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 366, 398 |
| BbrPI | CACGTG | 2 cut(s) | 1085, 3251 |
| BbsI | GAAGAC | 1 cut(s) | 645 |
| Bbv12I | GWGCWC | 2 cut(s) | 1166, 3469 |
| BbvCI | CCTCAGC | 2 cut(s) | 1242, 1657 |
| BccI | CCATC | 7 cut(s) | 247, 767, 886, 1639, 2562, 2763, 3077 |
| BceAI | ACGGC | 3 cut(s) | 1327, 2348, 3314 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 377, 411, 1058, 1092 |
| BciT130I | CCWGG | 2 cut(s) | 3119, 3158 |
| BclI | TGATCA | 1 cut(s) | 1030 |
| BcoDI | GTCTC | 9 cut(s) | 371, 456, 564, 573, 957, 1946, 1963, 2948, 3179 |
| BcuI | ACTAGT | 2 cut(s) | 1852, 3140 |
| BfoI | RGCGCY | 2 cut(s) | 823, 1850 |
| BfuAI | ACCTGC | 1 cut(s) | 2592 |
| BglII | AGATCT | 3 cut(s) | 92, 784, 3149 |
| BlpI | GCTNAGC | 3 cut(s) | 686, 1602, 3468 |
| BmcAI | AGTACT | 1 cut(s) | 865 |
| Bme18I | GGWCC | 1 cut(s) | 434 |
| BmeT110I | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| BmgT120I | GGNCC | 4 cut(s) | 434, 1130, 2511, 3362 |
| BmiI | GGNNCC | 5 cut(s) | 29, 120, 616, 708, 3353 |
| BmrI | ACTGGG | 2 cut(s) | 711, 3561 |
| BmuI | ACTGGG | 2 cut(s) | 711, 3561 |
| BpiI | GAAGAC | 1 cut(s) | 645 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1943, 1975, 3004, 3036 |
| BpmI | CTGGAG | 3 cut(s) | 633, 1140, 2381 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1242, 1657 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 686, 1602, 3468 |
| BpuEI | CTTGAG | 7 cut(s) | 891, 1114, 1560, 1945, 2045, 2909, 3031 |
| BsaAI | YACGTR | 2 cut(s) | 1085, 3251 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2912 |
| BsaHI | GRCGYC | 2 cut(s) | 152, 726 |
| BsaI | GGTCTC | 1 cut(s) | 564 |
| BsaWI | WCCGGW | 2 cut(s) | 441, 1812 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 136, 678, 1095, 1096 |
| Bse118I | RCCGGY | 1 cut(s) | 56 |
| Bse1I | ACTGG | 5 cut(s) | 706, 1052, 1157, 2364, 3556 |
| Bse21I | CCTNAGG | 1 cut(s) | 356 |
| Bse3DI | GCAATG | 3 cut(s) | 288, 1257, 1891 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2912 |
| BseBI | CCWGG | 2 cut(s) | 3119, 3158 |
| BseGI | GGATG | 5 cut(s) | 239, 1357, 2095, 3088, 3127 |
| BseJI | GATNNNNATC | 1 cut(s) | 2912 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 136, 678, 1095, 1096 |
| BseMI | GCAATG | 3 cut(s) | 288, 1257, 1891 |
| BseMII | CTCAG | 8 cut(s) | 80, 215, 285, 826, 1233, 1266, 1671, 3482 |
| BseNI | ACTGG | 5 cut(s) | 706, 1052, 1157, 2364, 3556 |
| BseRI | GAGGAG | 5 cut(s) | 78, 81, 90, 1410, 1645 |
| BseSI | GKGCMC | 1 cut(s) | 1090 |
| BseYI | CCCAGC | 2 cut(s) | 368, 2838 |
| BsgI | GTGCAG | 5 cut(s) | 678, 963, 2487, 3186, 3318 |
| Bsh1236I | CGCG | 2 cut(s) | 894, 3406 |
| BshFI | GGCC | 7 cut(s) | 56, 164, 1131, 2513, 3156, 3277, 3364 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1166, 3469 |
| BsiHKCI | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| BslFI | GGGAC | 5 cut(s) | 541, 1158, 1160, 1423, 3357 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 136, 678, 1095, 1096 |
| BsmAI | GTCTC | 9 cut(s) | 371, 456, 564, 573, 957, 1946, 1963, 2948, 3179 |
| BsmFI | GGGAC | 5 cut(s) | 541, 1158, 1160, 1423, 3357 |
| BsmI | GAATGC | 2 cut(s) | 2521, 2566 |
| BsnI | GGCC | 7 cut(s) | 56, 164, 1131, 2513, 3156, 3277, 3364 |
| Bso31I | GGTCTC | 1 cut(s) | 564 |
| BsoBI | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| Bsp1286I | GDGCHC | 5 cut(s) | 32, 619, 1090, 1166, 3469 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 686, 1602, 3468 |
| Bsp68I | TCGCGA | 1 cut(s) | 3406 |
| BspANI | GGCC | 7 cut(s) | 56, 164, 1131, 2513, 3156, 3277, 3364 |
| BspCNI | CTCAG | 8 cut(s) | 81, 216, 286, 825, 1234, 1267, 1670, 3481 |
| BspFNI | CGCG | 2 cut(s) | 894, 3406 |
| BspHI | TCATGA | 3 cut(s) | 2674, 2802, 2916 |
| BspLI | GGNNCC | 5 cut(s) | 29, 120, 616, 708, 3353 |
| BspMAI | CTGCAG | 4 cut(s) | 316, 390, 1267, 2686 |
| BspMI | ACCTGC | 1 cut(s) | 2592 |
| BspPI | GGATC | 1 cut(s) | 663 |
| BspQI | GCTCTTC | 1 cut(s) | 2214 |
| BspTNI | GGTCTC | 1 cut(s) | 564 |
| BsrDI | GCAATG | 3 cut(s) | 288, 1257, 1891 |
| BsrFI | RCCGGY | 1 cut(s) | 56 |
| BsrI | ACTGG | 5 cut(s) | 706, 1052, 1157, 2364, 3556 |
| BssAI | RCCGGY | 1 cut(s) | 56 |
| BssNI | GRCGYC | 2 cut(s) | 152, 726 |
| BssT1I | CCWWGG | 4 cut(s) | 581, 992, 1504, 2934 |
| Bst2UI | CCWGG | 2 cut(s) | 3119, 3158 |
| Bst4CI | ACNGT | 8 cut(s) | 17, 1038, 1211, 1309, 1595, 1957, 2327, 2855 |
| Bst6I | CTCTTC | 3 cut(s) | 369, 1725, 2214 |
| BstACI | GRCGYC | 2 cut(s) | 152, 726 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 320 |
| BstBAI | YACGTR | 2 cut(s) | 1085, 3251 |
| BstF5I | GGATG | 5 cut(s) | 239, 1357, 2095, 3088, 3127 |
| BstFNI | CGCG | 2 cut(s) | 894, 3406 |
| BstH2I | RGCGCY | 2 cut(s) | 823, 1850 |
| BstHHI | GCGC | 5 cut(s) | 129, 306, 822, 1230, 1849 |
| BstMAI | GTCTC | 9 cut(s) | 371, 456, 564, 573, 957, 1946, 1963, 2948, 3179 |
| BstNI | CCWGG | 2 cut(s) | 3119, 3158 |
| BstNSI | RCATGY | 7 cut(s) | 875, 1393, 1473, 1912, 1924, 2154, 2647 |
| BstSLI | GKGCMC | 1 cut(s) | 1090 |
| BstUI | CGCG | 2 cut(s) | 894, 3406 |
| BstV2I | GAAGAC | 1 cut(s) | 645 |
| BstX2I | RGATCY | 4 cut(s) | 92, 668, 784, 3149 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3241 |
| BstYI | RGATCY | 4 cut(s) | 92, 668, 784, 3149 |
| Bsu36I | CCTNAGG | 1 cut(s) | 356 |
| BsuRI | GGCC | 7 cut(s) | 56, 164, 1131, 2513, 3156, 3277, 3364 |
| BtgZI | GCGATG | 2 cut(s) | 2075, 2196 |
| BtsCI | GGATG | 5 cut(s) | 239, 1357, 2095, 3088, 3127 |
| BtsI | GCAGTG | 3 cut(s) | 1365, 2910, 3203 |
| BtsIMutI | CAGTG | 9 cut(s) | 699, 1164, 1216, 1365, 1600, 2332, 2860, 2910, 3203 |
| BtuMI | TCGCGA | 1 cut(s) | 3406 |
| BveI | ACCTGC | 1 cut(s) | 2592 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1637 |
| CciI | TCATGA | 3 cut(s) | 2674, 2802, 2916 |
| CfoI | GCGC | 5 cut(s) | 129, 306, 822, 1230, 1849 |
| Cfr10I | RCCGGY | 1 cut(s) | 56 |
| Cfr13I | GGNCC | 4 cut(s) | 434, 1130, 2511, 3362 |
| Cfr9I | CCCGGG | 4 cut(s) | 32, 598, 1176, 3359 |
| CseI | GACGC | 4 cut(s) | 141, 462, 1354, 2217 |
| Csp6I | GTAC | 7 cut(s) | 361, 509, 864, 1934, 1965, 2357, 3433 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 312, 347 |
| CviQI | GTAC | 7 cut(s) | 361, 509, 864, 1934, 1965, 2357, 3433 |
| DraI | TTTAAA | 1 cut(s) | 1689 |
| EaeI | YGGCCR | 4 cut(s) | 54, 162, 3154, 3275 |
| Eam1104I | CTCTTC | 3 cut(s) | 369, 1725, 2214 |
| EarI | CTCTTC | 3 cut(s) | 369, 1725, 2214 |
| EciI | GGCGGA | 2 cut(s) | 75, 155 |
| Ecl136II | GAGCTC | 1 cut(s) | 3467 |
| Eco130I | CCWWGG | 4 cut(s) | 581, 992, 1504, 2934 |
| Eco24I | GRGCYC | 3 cut(s) | 32, 619, 3469 |
| Eco31I | GGTCTC | 1 cut(s) | 564 |
| Eco32I | GATATC | 1 cut(s) | 2318 |
| Eco47I | GGWCC | 1 cut(s) | 434 |
| Eco53kI | GAGCTC | 1 cut(s) | 3467 |
| Eco57I | CTGAAG | 9 cut(s) | 32, 1424, 1959, 2253, 2676, 2688, 3431, 3494, 3512 |
| Eco72I | CACGTG | 2 cut(s) | 1085, 3251 |
| Eco81I | CCTNAGG | 1 cut(s) | 356 |
| Eco88I | CYCGRG | 4 cut(s) | 32, 598, 1176, 3359 |
| EcoICRI | GAGCTC | 1 cut(s) | 3467 |
| EcoRI | GAATTC | 4 cut(s) | 219, 1870, 2290, 2614 |
| EcoRII | CCWGG | 2 cut(s) | 3117, 3156 |
| EcoRV | GATATC | 1 cut(s) | 2318 |
| EcoT14I | CCWWGG | 4 cut(s) | 581, 992, 1504, 2934 |
| EcoT22I | ATGCAT | 1 cut(s) | 2156 |
| EcoT38I | GRGCYC | 3 cut(s) | 32, 619, 3469 |
| ErhI | CCWWGG | 4 cut(s) | 581, 992, 1504, 2934 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1590, 1622 |
| FaqI | GGGAC | 5 cut(s) | 541, 1158, 1160, 1423, 3357 |
| FauNDI | CATATG | 2 cut(s) | 2418, 3441 |
| FbaI | TGATCA | 1 cut(s) | 1030 |
| FblI | GTMKAC | 1 cut(s) | 12 |
| FokI | GGATG | 5 cut(s) | 226, 1364, 2102, 3095, 3134 |
| FriOI | GRGCYC | 3 cut(s) | 32, 619, 3469 |
| GlaI | GCGC | 5 cut(s) | 128, 305, 821, 1229, 1848 |
| GsaI | CCCAGC | 2 cut(s) | 372, 2842 |
| GsuI | CTGGAG | 3 cut(s) | 633, 1140, 2381 |
| HaeII | RGCGCY | 2 cut(s) | 823, 1850 |
| HaeIII | GGCC | 7 cut(s) | 56, 164, 1131, 2513, 3156, 3277, 3364 |
| HgaI | GACGC | 4 cut(s) | 141, 462, 1354, 2217 |
| HhaI | GCGC | 5 cut(s) | 129, 306, 822, 1230, 1849 |
| Hin1I | GRCGYC | 2 cut(s) | 152, 726 |
| Hin6I | GCGC | 5 cut(s) | 127, 304, 820, 1228, 1847 |
| HinP1I | GCGC | 5 cut(s) | 127, 304, 820, 1228, 1847 |
| HincII | GTYRAC | 2 cut(s) | 477, 989 |
| HindII | GTYRAC | 2 cut(s) | 477, 989 |
| HindIII | AAGCTT | 4 cut(s) | 210, 1604, 1898, 2255 |
| HphI | GGTGA | 3 cut(s) | 1041, 1278, 2275 |
| Hpy166II | GTNNAC | 6 cut(s) | 13, 477, 989, 1214, 2330, 2357 |
| Hpy8I | GTNNAC | 6 cut(s) | 13, 477, 989, 1214, 2330, 2357 |
| Hpy99I | CGWCG | 5 cut(s) | 10, 103, 157, 160, 809 |
| HpyAV | CCTTC | 9 cut(s) | 650, 682, 1056, 2456, 2712, 3088, 3365, 3397, 3518 |
| HpyCH4III | ACNGT | 8 cut(s) | 17, 1038, 1211, 1309, 1595, 1957, 2327, 2855 |
| HpyCH4IV | ACGT | 5 cut(s) | 726, 807, 1084, 3058, 3250 |
| HpySE526I | ACGT | 5 cut(s) | 726, 807, 1084, 3058, 3250 |
| Hsp92I | GRCGYC | 2 cut(s) | 152, 726 |
| HspAI | GCGC | 5 cut(s) | 127, 304, 820, 1228, 1847 |
| KroI | GCCGGC | 1 cut(s) | 56 |
| KroNI | GCCGGC | 1 cut(s) | 58 |
| Ksp22I | TGATCA | 1 cut(s) | 1030 |
| LguI | GCTCTTC | 1 cut(s) | 2214 |
| LmnI | GCTCC | 7 cut(s) | 27, 118, 301, 614, 1423, 1658, 1984 |
| MaeII | ACGT | 5 cut(s) | 726, 807, 1084, 3058, 3250 |
| MfeI | CAATTG | 3 cut(s) | 1557, 3002, 3239 |
| MflI | RGATCY | 4 cut(s) | 92, 668, 784, 3149 |
| MhlI | GDGCHC | 5 cut(s) | 32, 619, 1090, 1166, 3469 |
| MlsI | TGGCCA | 2 cut(s) | 3156, 3277 |
| MluNI | TGGCCA | 2 cut(s) | 3156, 3277 |
| MlyI | GAGTC | 1 cut(s) | 523 |
| MmeI | TCCRAC | 5 cut(s) | 5, 940, 1484, 2328, 3313 |
| Mox20I | TGGCCA | 2 cut(s) | 3156, 3277 |
| Mph1103I | ATGCAT | 1 cut(s) | 2156 |
| MroNI | GCCGGC | 1 cut(s) | 56 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1887, 2796 |
| MscI | TGGCCA | 2 cut(s) | 3156, 3277 |
| MslI | CAYNNNNRTG | 5 cut(s) | 600, 734, 1209, 2559, 2853 |
| Msp20I | TGGCCA | 2 cut(s) | 3156, 3277 |
| MspA1I | CMGCKG | 3 cut(s) | 124, 1241, 3532 |
| MunI | CAATTG | 3 cut(s) | 1557, 3002, 3239 |
| Mva1269I | GAATGC | 2 cut(s) | 2521, 2566 |
| MvaI | CCWGG | 2 cut(s) | 3119, 3158 |
| MvnI | CGCG | 2 cut(s) | 894, 3406 |
| NaeI | GCCGGC | 1 cut(s) | 58 |
| NdeI | CATATG | 2 cut(s) | 2418, 3441 |
| NgoMIV | GCCGGC | 1 cut(s) | 56 |
| NlaIV | GGNNCC | 5 cut(s) | 29, 120, 616, 708, 3353 |
| NmuCI | GTSAC | 4 cut(s) | 459, 727, 1202, 2984 |
| NruI | TCGCGA | 1 cut(s) | 3406 |
| NsiI | ATGCAT | 1 cut(s) | 2156 |
| NspI | RCATGY | 7 cut(s) | 875, 1393, 1473, 1912, 1924, 2154, 2647 |
| OliI | CACNNNNGTG | 2 cut(s) | 600, 1209 |
| PaeI | GCATGC | 1 cut(s) | 2154 |
| PagI | TCATGA | 3 cut(s) | 2674, 2802, 2916 |
| PciI | ACATGT | 4 cut(s) | 871, 1389, 1469, 1920 |
| PciSI | GCTCTTC | 1 cut(s) | 2214 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 804 |
| PctI | GAATGC | 2 cut(s) | 2521, 2566 |
| PdiI | GCCGGC | 1 cut(s) | 58 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1887, 2796 |
| PleI | GAGTC | 1 cut(s) | 523 |
| PmaCI | CACGTG | 2 cut(s) | 1085, 3251 |
| PmlI | CACGTG | 2 cut(s) | 1085, 3251 |
| PpsI | GAGTC | 1 cut(s) | 523 |
| Ppu21I | YACGTR | 2 cut(s) | 1085, 3251 |
| PscI | ACATGT | 4 cut(s) | 871, 1389, 1469, 1920 |
| PshBI | ATTAAT | 2 cut(s) | 2142, 2949 |
| Psp124BI | GAGCTC | 1 cut(s) | 3469 |
| Psp6I | CCWGG | 2 cut(s) | 3117, 3156 |
| PspCI | CACGTG | 2 cut(s) | 1085, 3251 |
| PspFI | CCCAGC | 2 cut(s) | 368, 2838 |
| PspGI | CCWGG | 2 cut(s) | 3117, 3156 |
| PspN4I | GGNNCC | 5 cut(s) | 29, 120, 616, 708, 3353 |
| PspPI | GGNCC | 4 cut(s) | 434, 1130, 2511, 3362 |
| PstI | CTGCAG | 4 cut(s) | 316, 390, 1267, 2686 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1637 |
| PsuI | RGATCY | 4 cut(s) | 92, 668, 784, 3149 |
| PvuII | CAGCTG | 2 cut(s) | 1241, 3532 |
| RruI | TCGCGA | 1 cut(s) | 3406 |
| RsaI | GTAC | 7 cut(s) | 362, 510, 865, 1935, 1966, 2358, 3434 |
| RsaNI | GTAC | 7 cut(s) | 361, 509, 864, 1934, 1965, 2357, 3433 |
| RseI | CAYNNNNRTG | 5 cut(s) | 600, 734, 1209, 2559, 2853 |
| SacI | GAGCTC | 1 cut(s) | 3469 |
| SapI | GCTCTTC | 1 cut(s) | 2214 |
| Sau96I | GGNCC | 4 cut(s) | 434, 1130, 2511, 3362 |
| ScaI | AGTACT | 1 cut(s) | 865 |
| SchI | GAGTC | 1 cut(s) | 523 |
| SduI | GDGCHC | 5 cut(s) | 32, 619, 1090, 1166, 3469 |
| SinI | GGWCC | 1 cut(s) | 434 |
| SmaI | CCCGGG | 4 cut(s) | 34, 600, 1178, 3361 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 600, 734, 1209, 2559, 2853 |
| SmlI | CTYRAG | 7 cut(s) | 906, 1093, 1575, 1960, 2024, 2888, 3010 |
| SmoI | CTYRAG | 7 cut(s) | 906, 1093, 1575, 1960, 2024, 2888, 3010 |
| SpeI | ACTAGT | 2 cut(s) | 1852, 3140 |
| SphI | GCATGC | 1 cut(s) | 2154 |
| SspI | AATATT | 6 cut(s) | 760, 1693, 1803, 2545, 2635, 2971 |
| SstI | GAGCTC | 1 cut(s) | 3469 |
| StyI | CCWWGG | 4 cut(s) | 581, 992, 1504, 2934 |
| TaaI | ACNGT | 8 cut(s) | 17, 1038, 1211, 1309, 1595, 1957, 2327, 2855 |
| TaiI | ACGT | 5 cut(s) | 729, 810, 1087, 3061, 3253 |
| TaqI | TCGA | 5 cut(s) | 41, 101, 215, 804, 3214 |
| TatI | WGTACW | 2 cut(s) | 863, 2356 |
| TauI | GCSGC | 4 cut(s) | 115, 124, 164, 1082 |
| TscAI | CASTG | 9 cut(s) | 706, 1164, 1216, 1365, 1600, 2332, 2860, 2910, 3210 |
| TseFI | GTSAC | 4 cut(s) | 459, 727, 1202, 2984 |
| Tsp45I | GTSAC | 4 cut(s) | 459, 727, 1202, 2984 |
| TspGWI | ACGGA | 1 cut(s) | 2420 |
| TspMI | CCCGGG | 4 cut(s) | 32, 598, 1176, 3359 |
| TspRI | CASTG | 9 cut(s) | 706, 1164, 1216, 1365, 1600, 2332, 2860, 2910, 3210 |
| VpaK11BI | GGWCC | 1 cut(s) | 434 |
| VspI | ATTAAT | 2 cut(s) | 2142, 2949 |
| XapI | RAATTY | 8 cut(s) | 219, 901, 1870, 2290, 2366, 2614, 2655, 2814 |
| XbaI | TCTAGA | 2 cut(s) | 1316, 2312 |
| XceI | RCATGY | 7 cut(s) | 875, 1393, 1473, 1912, 1924, 2154, 2647 |
| XmaI | CCCGGG | 4 cut(s) | 32, 598, 1176, 3359 |
| XmiI | GTMKAC | 1 cut(s) | 12 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1887, 2796 |
| ZraI | GACGTC | 1 cut(s) | 727 |
| ZrmI | AGTACT | 1 cut(s) | 865 |
| Zsp2I | ATGCAT | 1 cut(s) | 2156 |