FvH4_7g22670

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004953
Physical Location & Seq
Forward (+)
17845708 .. 17848631
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22670.t2

Sequence Viewer

Length: 1575 bp
ATGAAACTCGAAGAGTTTCTAATGCAACGAAGCGAGGAGAAGAAGAAGAGACTTGTTCTTGAGGAAGAGGTTCTGAAATTGCAAGAAGAGTTGGATGGGGAGCAAACCCTGAATAGGGTTCTTCACTGTGCACTTCATGGACCGGTTCTGTCACACCCATGTCTTCTCTCATTGCTTCCACCTCAGGTTCAGGAGCTATTTTCGGAGCTGGAAATGGTAGAAGATGAGATCAGTGGGCTCGAAAGAAAAATGGAAGAGCTGAAACTGATGTTGTATAAAGAGAGAGTTCAAACAAGAGAGTGGGAGATGCATAGGAGAAAGTGGGAACAGAATCAAGCATTGTTCAGAGCTGGAAATCAGTCACTGATTGATGGATCGAGATCGCAGAACTACGAAGCATTGAGAAGTGAAAAAAGGATAGTAAGGGATAGAAGAGCCTCTGTTGGTTCTGCTTCTGATATGCAAAGGTGGAATTACACAAAAACTGATGCAGGAGAGATTGCGGAAATGTCAAGGAGACTAAGCAGAAGAGGCAGAATTCAAAGCGTTGGGGACAATGAAAGTGGCATTCTGAAACCAAATGAACTCTCGGAACAGTTGCTCAAGTGCCTTATAGGCATATTTCTTGAATTAAAACAGACCTCATTGGACAAAGGGGGGTCATCCTCTGTTGTTCCAAAGCTTGCACTCTCTTGTATGAACTCAAAAGGGTTCATGCCGAAAACTTCATTCAACTGCAAATCATCCGCGATCTTCAACTACAACACATCACATATTGATCCTTACTGCATTTTGCCAGATATAGATGGTGCTGTCCGCGACGTTGGCCCATATAAGAACTTCATCCAAATTACAAGAAACTCATTAGACATCAGACGCTTATCAGATTGTTCAAATGGAATTGAAAAATTGAGGAAATTGATGCATCAGTTGTGTGATGTGGACTTGACTTACTTGACCTACAAGCAGAAGCTAGCATTCTGGATCAACATCTACAATGCCTGCATCATGCATGCATTTCTGGAACATGGGTTGCCTTCTACACAAGAAAAGCTACTTGCACTTATGAACAAGGCTGCACTGAATGTCGGCGGCATAGTGCTAAACTCTCTAGCTATCGAGCATTTCATACTGCGACATCCATCAGAAACGAAACATGGACCTGCTTATGAGAAAGAAATGCTACTAAGACATGCCTATGGTTTAGGATACCCTGAACCAAATGTGACCTTTGCTCTTTGCAGAGGAAGTTGGTCCTCACCAGCATTAAGGGTCTATACCTCAGACGAGATAGTGAATGAGCTAGAGAGAGCCAAGGTAGAGTACTTGGAAGCTTCAGTAGGAGTTACTAGCAAGAAAAGAATCATGCTGCCAAAGCTTCTACAATGGCACATGCGCGATTTTGCAGATGATATAGACTCACTGCTAGAATGGATTTACAGCCAATTGCCGCGATCGGGGTCGCTGAAAAGATTGATAATGGAGTGCTTGATAGGGGAAACAAAGTCACCGGTAAACAAAATTGTTGAAGTCCAACCTCATGAGTCTGAGTTCCGCTATTTACTACCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

525

Amino Acids

60.23

Weight (kDa)

7.58

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 13 - 94 7.4e-17 Leucine-zipper of ternary complex factor MIP1
DUF547 PF04784 318 - 442 4.3e-38 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1171
AccII CGCG 4 cut(s) 749, 819, 1398, 1453
AciI CCGC 6 cut(s) 503, 747, 817, 1092, 1451, 1555
AclWI GGATC 3 cut(s) 382, 773, 992
AcsI RAATTY 1 cut(s) 537
AcuI CTGAAG 1 cut(s) 1320
AfaI GTAC 1 cut(s) 1325
AfiI CCNNNNNNNGG 3 cut(s) 114, 115, 1457
AgeI ACCGGT 2 cut(s) 142, 1510
AgsI TTSAA 8 cut(s) 290, 542, 629, 733, 757, 894, 905, 1529
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 2 cut(s) 43, 511
Alw44I GTGCAC 1 cut(s) 129
AlwI GGATC 3 cut(s) 382, 773, 992
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 1 cut(s) 826
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 1076, 1369
ApoI RAATTY 1 cut(s) 537
AsiGI ACCGGT 2 cut(s) 142, 1510
Asp700I GAANNNNTTC 2 cut(s) 15, 69
AspLEI GCGC 1 cut(s) 1398
AspS9I GGNCC 4 cut(s) 140, 827, 1160, 1254
AsuHPI GGTGA 2 cut(s) 1251, 1500
AsuNHI GCTAGC 1 cut(s) 973
AvaII GGWCC 3 cut(s) 140, 1160, 1254
AxyI CCTNAGG 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 1063, 1356
BccI CCATC 4 cut(s) 89, 365, 800, 1150
BciVI GTATCC 1 cut(s) 1202
BcoDI GTCTC 2 cut(s) 43, 511
BfaI CTAG 5 cut(s) 974, 1112, 1304, 1350, 1427
BfuAI ACCTGC 1 cut(s) 1171
BfuI GTATCC 1 cut(s) 1202
BglI GCCNNNNNGGC 1 cut(s) 615
BisI GCNGC 4 cut(s) 1077, 1093, 1370, 1451
BlsI GCNGC 4 cut(s) 1078, 1094, 1371, 1452
BmcAI AGTACT 1 cut(s) 1325
Bme18I GGWCC 3 cut(s) 140, 1160, 1254
BmgT120I GGNCC 4 cut(s) 140, 827, 1160, 1254
BmsI GCATC 5 cut(s) 297, 478, 912, 934, 1014
BmtI GCTAGC 1 cut(s) 977
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 80, 587
BsaBI GATNNNNATC 2 cut(s) 379, 989
BsaJI CCNNGG 1 cut(s) 1314
BsaWI WCCGGW 2 cut(s) 142, 1510
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 115, 1457
Bse118I RCCGGY 2 cut(s) 142, 1510
Bse21I CCTNAGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 170
Bse8I GATNNNNATC 2 cut(s) 379, 989
BseDI CCNNGG 1 cut(s) 1314
BseGI GGATG 5 cut(s) 100, 662, 743, 843, 1138
BseJI GATNNNNATC 2 cut(s) 379, 989
BseLI CCNNNNNNNGG 3 cut(s) 114, 115, 1457
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 3 cut(s) 197, 1296, 1539
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 2 cut(s) 1063, 1356
BsgI GTGCAG 1 cut(s) 1062
Bsh1236I CGCG 4 cut(s) 749, 819, 1398, 1453
Bsh1285I CGRYCG 1 cut(s) 1457
BshFI GGCC 1 cut(s) 828
BshTI ACCGGT 2 cut(s) 142, 1510
BsiEI CGRYCG 1 cut(s) 1457
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 2 cut(s) 143, 1511
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 3 cut(s) 114, 115, 1457
BsmAI GTCTC 2 cut(s) 43, 511
BsmFI GGGAC 1 cut(s) 566
BsmI GAATGC 2 cut(s) 567, 977
BsnI GGCC 1 cut(s) 828
Bsp1286I GDGCHC 2 cut(s) 133, 240
Bsp143I GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
BspACI CCGC 6 cut(s) 503, 747, 817, 1092, 1451, 1555
BspANI GGCC 1 cut(s) 828
BspCNI CTCAG 3 cut(s) 196, 1295, 1540
BspFNI CGCG 4 cut(s) 749, 819, 1398, 1453
BspHI TCATGA 1 cut(s) 1540
BspMI ACCTGC 1 cut(s) 1171
BspOI GCTAGC 1 cut(s) 977
BspPI GGATC 3 cut(s) 382, 773, 992
BspQI GCTCTTC 2 cut(s) 249, 427
BsrDI GCAATG 1 cut(s) 170
BsrFI RCCGGY 2 cut(s) 142, 1510
BssAI RCCGGY 2 cut(s) 142, 1510
BssECI CCNNGG 1 cut(s) 1314
BssMI GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
BssT1I CCWWGG 1 cut(s) 1314
Bst4CI ACNGT 2 cut(s) 128, 597
Bst6I CTCTTC 7 cut(s) 6, 41, 60, 81, 249, 427, 523
BstC8I GCNNGC 4 cut(s) 684, 975, 1003, 1014
BstDEI CTNAG 5 cut(s) 183, 521, 1187, 1282, 1548
BstF5I GGATG 5 cut(s) 100, 662, 743, 843, 1138
BstFNI CGCG 4 cut(s) 749, 819, 1398, 1453
BstHHI GCGC 1 cut(s) 1398
BstKTI GATC 7 cut(s) 231, 377, 383, 753, 781, 987, 1457
BstMAI GTCTC 2 cut(s) 43, 511
BstMBI GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
BstMCI CGRYCG 1 cut(s) 1457
BstMWI GCNNNNNNNGC 4 cut(s) 531, 615, 825, 1375
BstNSI RCATGY 3 cut(s) 1016, 1196, 1396
BstSLI GKGCMC 1 cut(s) 133
BstUI CGCG 4 cut(s) 749, 819, 1398, 1453
BstV1I GCAGC 2 cut(s) 1063, 1356
BstV2I GAAGAC 1 cut(s) 155
Bsu36I CCTNAGG 1 cut(s) 183
BsuI GTATCC 1 cut(s) 1202
BsuRI GGCC 1 cut(s) 828
BtsCI GGATG 5 cut(s) 100, 662, 743, 843, 1138
BtsI GCAGTG 1 cut(s) 1421
BtsIMutI CAGTG 5 cut(s) 124, 238, 362, 1079, 1421
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 4 cut(s) 684, 975, 1003, 1014
CaiI CAGNNNCTG 1 cut(s) 364
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 1 cut(s) 1398
Cfr10I RCCGGY 2 cut(s) 142, 1510
Cfr13I GGNCC 4 cut(s) 140, 827, 1160, 1254
CseI GACGC 1 cut(s) 885
Csp6I GTAC 1 cut(s) 1324
CspAI ACCGGT 2 cut(s) 142, 1510
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CviQI GTAC 1 cut(s) 1324
DdeI CTNAG 5 cut(s) 183, 521, 1187, 1282, 1548
DpnI GATC 7 cut(s) 230, 376, 382, 752, 780, 986, 1456
DpnII GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
Eam1104I CTCTTC 7 cut(s) 6, 41, 60, 81, 249, 427, 523
EarI CTCTTC 7 cut(s) 6, 41, 60, 81, 249, 427, 523
Eco130I CCWWGG 1 cut(s) 1314
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 3 cut(s) 140, 1160, 1254
Eco57I CTGAAG 1 cut(s) 1320
Eco81I CCTNAGG 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 537
EcoT14I CCWWGG 1 cut(s) 1314
EcoT22I ATGCAT 4 cut(s) 312, 927, 1014, 1018
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 1314
FaqI GGGAC 1 cut(s) 566
Fnu4HI GCNGC 4 cut(s) 1077, 1093, 1370, 1451
FokI GGATG 5 cut(s) 107, 649, 730, 830, 1125
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 4 cut(s) 1077, 1093, 1370, 1451
FspBI CTAG 5 cut(s) 974, 1112, 1304, 1350, 1427
GlaI GCGC 1 cut(s) 1397
GluI GCNGC 4 cut(s) 1077, 1093, 1370, 1451
HaeIII GGCC 1 cut(s) 828
HapII CCGG 2 cut(s) 143, 1511
HgaI GACGC 1 cut(s) 885
HhaI GCGC 1 cut(s) 1398
Hin6I GCGC 1 cut(s) 1396
HinP1I GCGC 1 cut(s) 1396
HindIII AAGCTT 3 cut(s) 680, 1332, 1376
HinfI GANTC 4 cut(s) 331, 1362, 1418, 1544
HpaII CCGG 2 cut(s) 143, 1511
HphI GGTGA 2 cut(s) 1251, 1500
Hpy166II GTNNAC 3 cut(s) 131, 943, 1516
Hpy188III TCNNGA 7 cut(s) 59, 191, 378, 626, 982, 1022, 1541
Hpy8I GTNNAC 3 cut(s) 131, 943, 1516
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 1 cut(s) 1047
HpyCH4III ACNGT 2 cut(s) 128, 597
HpyCH4IV ACGT 1 cut(s) 822
HpyF10VI GCNNNNNNNGC 4 cut(s) 531, 615, 825, 1375
HpyF3I CTNAG 5 cut(s) 183, 521, 1187, 1282, 1548
HpySE526I ACGT 1 cut(s) 822
HspAI GCGC 1 cut(s) 1396
Kzo9I GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
LguI GCTCTTC 2 cut(s) 249, 427
LmnI GCTCC 3 cut(s) 100, 193, 205
Lsp1109I GCAGC 2 cut(s) 1063, 1356
LweI GCATC 5 cut(s) 297, 478, 912, 934, 1014
MaeI CTAG 5 cut(s) 974, 1112, 1304, 1350, 1427
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 5 cut(s) 150, 360, 1225, 1345, 1506
MalI GATC 7 cut(s) 230, 376, 382, 752, 780, 986, 1456
MboI GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
MfeI CAATTG 1 cut(s) 1445
MhlI GDGCHC 2 cut(s) 133, 240
MlyI GAGTC 2 cut(s) 1412, 1553
MmeI TCCRAC 2 cut(s) 72, 1558
Mph1103I ATGCAT 4 cut(s) 312, 927, 1014, 1018
MroXI GAANNNNTTC 2 cut(s) 15, 69
MseI TTAA 2 cut(s) 632, 1268
MslI CAYNNNNRTG 2 cut(s) 157, 1197
MspI CCGG 2 cut(s) 143, 1511
MunI CAATTG 1 cut(s) 1445
Mva1269I GAATGC 2 cut(s) 567, 977
MvnI CGCG 4 cut(s) 749, 819, 1398, 1453
MwoI GCNNNNNNNGC 4 cut(s) 531, 615, 825, 1375
NdeII GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
NheI GCTAGC 1 cut(s) 973
NmuCI GTSAC 4 cut(s) 150, 360, 1225, 1506
NsiI ATGCAT 4 cut(s) 312, 927, 1014, 1018
NspI RCATGY 3 cut(s) 1016, 1196, 1396
PaeI GCATGC 1 cut(s) 1016
PagI TCATGA 1 cut(s) 1540
PciSI GCTCTTC 2 cut(s) 249, 427
PctI GAATGC 2 cut(s) 567, 977
PdmI GAANNNNTTC 2 cut(s) 15, 69
PfeI GAWTC 2 cut(s) 331, 1362
PinAI ACCGGT 2 cut(s) 142, 1510
PkrI GCNGC 4 cut(s) 1078, 1094, 1371, 1452
Ple19I CGATCG 1 cut(s) 1457
PleI GAGTC 2 cut(s) 1412, 1552
PpsI GAGTC 2 cut(s) 1412, 1552
PspPI GGNCC 4 cut(s) 140, 827, 1160, 1254
PstNI CAGNNNCTG 1 cut(s) 364
PvuI CGATCG 1 cut(s) 1457
RsaI GTAC 1 cut(s) 1325
RsaNI GTAC 1 cut(s) 1324
RseI CAYNNNNRTG 2 cut(s) 157, 1197
SapI GCTCTTC 2 cut(s) 249, 427
SaqAI TTAA 2 cut(s) 632, 1268
SatI GCNGC 4 cut(s) 1077, 1093, 1370, 1451
Sau3AI GATC 7 cut(s) 228, 374, 380, 750, 778, 984, 1454
Sau96I GGNCC 4 cut(s) 140, 827, 1160, 1254
ScaI AGTACT 1 cut(s) 1325
SchI GAGTC 2 cut(s) 1412, 1553
SduI GDGCHC 2 cut(s) 133, 240
SfaNI GCATC 5 cut(s) 297, 478, 912, 934, 1014
SinI GGWCC 3 cut(s) 140, 1160, 1254
SmiMI CAYNNNNRTG 2 cut(s) 157, 1197
SmlI CTYRAG 2 cut(s) 59, 602
SmoI CTYRAG 2 cut(s) 59, 602
SphI GCATGC 1 cut(s) 1016
SsiI CCGC 6 cut(s) 503, 747, 817, 1092, 1451, 1555
SspMI CTAG 5 cut(s) 974, 1112, 1304, 1350, 1427
StyI CCWWGG 1 cut(s) 1314
TaaI ACNGT 2 cut(s) 128, 597
TaiI ACGT 1 cut(s) 825
TaqI TCGA 4 cut(s) 9, 240, 377, 1119
TatI WGTACW 1 cut(s) 1323
TauI GCSGC 2 cut(s) 1095, 1453
TfiI GAWTC 2 cut(s) 331, 1362
Tru1I TTAA 2 cut(s) 632, 1268
Tru9I TTAA 2 cut(s) 632, 1268
TscAI CASTG 5 cut(s) 131, 238, 369, 1086, 1428
TseFI GTSAC 4 cut(s) 150, 360, 1225, 1506
TseI GCWGC 2 cut(s) 1076, 1369
Tsp45I GTSAC 4 cut(s) 150, 360, 1225, 1506
TspRI CASTG 5 cut(s) 131, 238, 369, 1086, 1428
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 3 cut(s) 140, 1160, 1254
XapI RAATTY 1 cut(s) 537
XceI RCATGY 3 cut(s) 1016, 1196, 1396
XmnI GAANNNNTTC 2 cut(s) 15, 69
XspI CTAG 5 cut(s) 974, 1112, 1304, 1350, 1427
ZrmI AGTACT 1 cut(s) 1325
Zsp2I ATGCAT 4 cut(s) 312, 927, 1014, 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.