MD07G1200000.v1.1

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004953
Physical Location & Seq
Forward (+)
27809963 .. 27812682
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1200000.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGAAATTAGAAGACTTTCTAATGCAACGAAACGAGGAGAAGCAGAGAGGACTTGATCTTGAGGAAGAGGTTAAGAAATTGCAGGCAAAGTTGGAAGGAGAACAAACACTAGGCAGGTTTCTGCGTTGTGCACTTCATGATCCCGTTCAGTCACATCCACGTCTTTTTTCCTTGCTTCCAGCTCAGGTTCAGGAGCTGTTTTCGGAGCTTGCAATGGTAGAGGAAGAGATAGGTCAACTTGACAGAAAAGTCGAAGAGCTGAAACTGAGGTTGTACAAAGAAAGGGAACAAACAAGAGAATGGAAAGTGCATATGTGGCAATTAGGGCAACAGAAGCAGTTGTTGTGTAGAGTGGGAAACCAGTCAGTGCTAAACGAAGAGAGATGCAGATCGCAGAACTATGAAGCTTTGAGAAATTTGAGAAAAGAAAGAAGGTTGAGGGATAGAAGAGCATCTGTGGGTTCTGCAGCAGATATCCCAAGGTGGAATTTCACCAAGTCCGATGGGATCATTTGTTGTCCCAAAGCTTGCTCTCTCCTGCATGAACTCGAAAGGGCTCATTCCGAAGACCTCAACATCCACAATTTCTTCAACTACAACACCTCATCCAATGCCGACCCTTATTGCATATTGCCTGACTTAGACGGCGCTGTCAGGGATGTTGGCCCGTATAAGAACTTAATCCAAATCACGAGGAGCTCGTTTGATGTCAGCCGCTTGTCTGAGTATTTGAATGGAATCGAAAAGTTGAGGAATTTGATGCATCAGCTGTGTGATGTGGACTTGACTTTCTTGACCTACAAGCAAAAGTTGGCATTCTGGATCAGCATCTACAATGCCTGCATCATGCATGGACCTGCCTATGGGAAGGAGATGCTACTAAGACATGCCTATGGTCTTGGATACCCCGAACCTTACGTCACATTTGCCCTTTGCCGCGGCAGTTGGTCCTCACCGGCACGAAACTGGAGTACTTGGAAGCTTCAGTGGAAGTCACAAGCAAGAAAGAAAATTGTGGTGCCCGAGCTTCTTCAATGGCACATGCTCGATTTTGCAGACAGTATGGAATCATTGCTGGAATGGATTTACAGCCAACTGCAGCGGTCTGGTTCACTGAAAAGATCGATAATCGAGTGCTTGAACGGAGAAACAAAGTCTCCGATCAGCAAAATGGTGGAAGTCCAACCTTATGAATCTGAGTTCCGGTACTTGTTGCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

408

Amino Acids

47.77

Weight (kDa)

8.2

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 13 - 94 6.7e-17 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 248, 650
Acc36I ACCTGC 2 cut(s) 105, 865
AccB1I GGYRCC 1 cut(s) 1018
AccII CGCG 1 cut(s) 939
AciI CCGC 4 cut(s) 715, 937, 939, 1102
AclWI GGATC 3 cut(s) 134, 515, 830
AcsI RAATTY 3 cut(s) 415, 487, 754
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 3 cut(s) 275, 973, 1208
AfiI CCNNNNNNNGG 1 cut(s) 863
AgsI TTSAA 4 cut(s) 592, 733, 1034, 1141
AjiI CACGTC 1 cut(s) 161
Alw21I GWGCWC 2 cut(s) 133, 701
Alw26I GTCTC 1 cut(s) 1161
Alw44I GTGCAC 1 cut(s) 129
AlwI GGATC 3 cut(s) 134, 515, 830
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 1022
AoxI GGCC 1 cut(s) 664
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 467, 1099
ApoI RAATTY 3 cut(s) 415, 487, 754
ArsI GACNNNNNNTTYG 2 cut(s) 903, 935
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 665, 854, 948
AsuHPI GGTGA 2 cut(s) 484, 945
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 2 cut(s) 854, 948
BaeGI GKGCMC 2 cut(s) 133, 1023
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 2 cut(s) 559, 701
BauI CACGAG 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 18, 573
Bbv12I GWGCWC 2 cut(s) 133, 701
BbvI GCAGC 2 cut(s) 479, 1111
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 661
BciVI GTATCC 1 cut(s) 896
BcoDI GTCTC 1 cut(s) 1161
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 2 cut(s) 465, 1097
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 2 cut(s) 105, 865
BfuI GTATCC 1 cut(s) 896
BisI GCNGC 5 cut(s) 468, 715, 937, 940, 1100
BlsI GCNGC 5 cut(s) 469, 716, 938, 941, 1101
BmcAI AGTACT 1 cut(s) 973
Bme18I GGWCC 2 cut(s) 854, 948
BmeT110I CYCGRG 1 cut(s) 1022
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 665, 854, 948
BmiI GGNNCC 1 cut(s) 1020
BmsI GCATC 7 cut(s) 374, 461, 750, 772, 837, 852, 864
BpiI GAAGAC 2 cut(s) 18, 573
BpmI CTGGAG 1 cut(s) 988
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 1124
BsaBI GATNNNNATC 2 cut(s) 388, 827
BsaJI CCNNGG 2 cut(s) 479, 937
BsaWI WCCGGW 1 cut(s) 1203
BsaXI ACNNNNNCTCC 2 cut(s) 1141, 1171
Bsc4I CCNNNNNNNGG 1 cut(s) 863
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 2 cut(s) 361, 971
Bse3DI GCAATG 2 cut(s) 219, 1070
Bse8I GATNNNNATC 2 cut(s) 388, 827
BseCI ATCGAT 1 cut(s) 1124
BseDI CCNNGG 2 cut(s) 479, 937
BseGI GGATG 4 cut(s) 154, 576, 605, 664
BseJI GATNNNNATC 2 cut(s) 388, 827
BseLI CCNNNNNNNGG 1 cut(s) 863
BseMI GCAATG 2 cut(s) 219, 1070
BseMII CTCAG 4 cut(s) 197, 257, 714, 1188
BseNI ACTGG 2 cut(s) 361, 971
BseRI GAGGAG 2 cut(s) 50, 709
BseSI GKGCMC 2 cut(s) 133, 1023
BseXI GCAGC 2 cut(s) 479, 1111
Bsh1236I CGCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 666
BshNI GGYRCC 1 cut(s) 1018
BshVI ATCGAT 1 cut(s) 1124
BsiHKAI GWGCWC 2 cut(s) 133, 701
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 2 cut(s) 956, 1204
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 863
BsmAI GTCTC 1 cut(s) 1161
BsmFI GGGAC 1 cut(s) 504
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 4 cut(s) 133, 559, 701, 1023
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
BspACI CCGC 4 cut(s) 715, 937, 939, 1102
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 4 cut(s) 196, 258, 715, 1189
BspDI ATCGAT 1 cut(s) 1124
BspFNI CGCG 1 cut(s) 939
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 1020
BspMAI CTGCAG 2 cut(s) 469, 1101
BspMI ACCTGC 2 cut(s) 105, 865
BspPI GGATC 3 cut(s) 134, 515, 830
BspQI GCTCTTC 2 cut(s) 249, 442
BspT107I GGYRCC 1 cut(s) 1018
BsrDI GCAATG 2 cut(s) 219, 1070
BsrFI RCCGGY 1 cut(s) 955
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 2 cut(s) 361, 971
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 2 cut(s) 479, 937
BssMI GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
BssSI CACGAG 1 cut(s) 691
BssT1I CCWWGG 1 cut(s) 479
Bst2BI CACGAG 1 cut(s) 691
Bst4CI ACNGT 1 cut(s) 1061
Bst6I CTCTTC 5 cut(s) 60, 219, 249, 372, 442
BstAUI TGTACA 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 84, 210, 529, 841
BstDEI CTNAG 6 cut(s) 183, 266, 640, 723, 881, 1197
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 4 cut(s) 154, 576, 605, 664
BstFNI CGCG 1 cut(s) 939
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 7 cut(s) 58, 142, 392, 510, 825, 1124, 1164
BstMAI GTCTC 1 cut(s) 1161
BstMBI GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 316, 325, 334
BstNSI RCATGY 2 cut(s) 890, 1045
BstSFI CTRYAG 2 cut(s) 465, 1097
BstSLI GKGCMC 2 cut(s) 133, 1023
BstUI CGCG 1 cut(s) 939
BstV1I GCAGC 2 cut(s) 479, 1111
BstV2I GAAGAC 2 cut(s) 18, 573
Bsu15I ATCGAT 1 cut(s) 1124
BsuI GTATCC 1 cut(s) 896
BsuRI GGCC 1 cut(s) 666
BsuTUI ATCGAT 1 cut(s) 1124
BtgI CCRYGG 1 cut(s) 937
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 4 cut(s) 154, 576, 605, 664
BtsIMutI CAGTG 3 cut(s) 372, 992, 1112
BveI ACCTGC 2 cut(s) 105, 865
Cac8I GCNNGC 4 cut(s) 84, 210, 529, 841
CaiI CAGNNNCTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 136
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 955
Cfr13I GGNCC 3 cut(s) 665, 854, 948
Cfr42I CCGCGG 1 cut(s) 940
ClaI ATCGAT 1 cut(s) 1124
Csp6I GTAC 3 cut(s) 274, 972, 1207
CviAII CATG 6 cut(s) 137, 542, 847, 851, 887, 1042
CviQI GTAC 3 cut(s) 274, 972, 1207
DdeI CTNAG 6 cut(s) 183, 266, 640, 723, 881, 1197
DpnI GATC 7 cut(s) 57, 141, 391, 509, 824, 1123, 1163
DpnII GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
DrdI GACNNNNNNGTC 2 cut(s) 248, 650
DseDI GACNNNNNNGTC 2 cut(s) 248, 650
Eam1104I CTCTTC 5 cut(s) 60, 219, 249, 372, 442
EarI CTCTTC 5 cut(s) 60, 219, 249, 372, 442
Ecl136II GAGCTC 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 479
Eco24I GRGCYC 2 cut(s) 559, 701
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 854, 948
Eco53kI GAGCTC 1 cut(s) 699
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 699
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 479
EcoT22I ATGCAT 2 cut(s) 765, 852
EcoT38I GRGCYC 2 cut(s) 559, 701
ErhI CCWWGG 1 cut(s) 479
FaeI CATG 6 cut(s) 140, 545, 850, 854, 890, 1045
FaqI GGGAC 1 cut(s) 504
FatI CATG 6 cut(s) 136, 541, 846, 850, 886, 1041
FauNDI CATATG 1 cut(s) 312
Fnu4HI GCNGC 5 cut(s) 468, 715, 937, 940, 1100
FokI GGATG 4 cut(s) 141, 563, 592, 671
FriOI GRGCYC 2 cut(s) 559, 701
Fsp4HI GCNGC 5 cut(s) 468, 715, 937, 940, 1100
FspBI CTAG 1 cut(s) 110
GlaI GCGC 1 cut(s) 649
GluI GCNGC 5 cut(s) 468, 715, 937, 940, 1100
GsuI CTGGAG 1 cut(s) 988
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 1 cut(s) 666
HapII CCGG 2 cut(s) 956, 1204
HhaI GCGC 1 cut(s) 650
Hin1II CATG 6 cut(s) 140, 545, 850, 854, 890, 1045
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HindIII AAGCTT 3 cut(s) 405, 525, 980
HinfI GANTC 3 cut(s) 738, 1067, 1193
HpaII CCGG 2 cut(s) 956, 1204
HphI GGTGA 2 cut(s) 484, 945
Hpy166II GTNNAC 4 cut(s) 131, 236, 781, 1112
Hpy188I TCNGA 6 cut(s) 205, 502, 565, 724, 1161, 1198
Hpy188III TCNNGA 6 cut(s) 59, 137, 191, 691, 793, 820
Hpy8I GTNNAC 4 cut(s) 131, 236, 781, 1112
HpyAV CCTTC 3 cut(s) 89, 426, 862
HpyCH4III ACNGT 1 cut(s) 1061
HpyCH4IV ACGT 2 cut(s) 160, 918
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 325, 334
HpyF3I CTNAG 6 cut(s) 183, 266, 640, 723, 881, 1197
HpySE526I ACGT 2 cut(s) 160, 918
Hsp92II CATG 6 cut(s) 140, 545, 850, 854, 890, 1045
HspAI GCGC 1 cut(s) 648
KspI CCGCGG 1 cut(s) 940
Kzo9I GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
LguI GCTCTTC 2 cut(s) 249, 442
LmnI GCTCC 3 cut(s) 193, 205, 696
Lsp1109I GCAGC 2 cut(s) 479, 1111
LweI GCATC 7 cut(s) 374, 461, 750, 772, 837, 852, 864
MaeI CTAG 1 cut(s) 110
MaeII ACGT 2 cut(s) 160, 918
MaeIII GTNAC 3 cut(s) 150, 919, 993
MalI GATC 7 cut(s) 57, 141, 391, 509, 824, 1123, 1163
MboI GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
MboII GAAGA 9 cut(s) 23, 77, 236, 266, 389, 459, 578, 580, 1022
MhlI GDGCHC 4 cut(s) 133, 559, 701, 1023
MluCI AATT 8 cut(s) 5, 77, 320, 415, 487, 583, 754, 1011
MmeI TCCRAC 2 cut(s) 72, 1207
Mph1103I ATGCAT 2 cut(s) 765, 852
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 2 cut(s) 72, 680
MslI CAYNNNNRTG 1 cut(s) 891
MspA1I CMGCKG 3 cut(s) 769, 939, 1102
MspI CCGG 2 cut(s) 956, 1204
Mva1269I GAATGC 1 cut(s) 815
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 3 cut(s) 316, 325, 334
NdeI CATATG 1 cut(s) 312
NdeII GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
NlaIII CATG 6 cut(s) 140, 545, 850, 854, 890, 1045
NlaIV GGNNCC 1 cut(s) 1020
NmuCI GTSAC 3 cut(s) 150, 919, 993
NsiI ATGCAT 2 cut(s) 765, 852
NspI RCATGY 2 cut(s) 890, 1045
PagI TCATGA 1 cut(s) 136
PciSI GCTCTTC 2 cut(s) 249, 442
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PctI GAATGC 1 cut(s) 815
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 3 cut(s) 738, 1067, 1193
PkrI GCNGC 5 cut(s) 469, 716, 938, 941, 1101
Psp124BI GAGCTC 1 cut(s) 701
PspN4I GGNNCC 1 cut(s) 1020
PspPI GGNCC 3 cut(s) 665, 854, 948
PstI CTGCAG 2 cut(s) 469, 1101
PstNI CAGNNNCTG 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 3 cut(s) 275, 973, 1208
RsaNI GTAC 3 cut(s) 274, 972, 1207
RseI CAYNNNNRTG 1 cut(s) 891
SacI GAGCTC 1 cut(s) 701
SacII CCGCGG 1 cut(s) 940
SapI GCTCTTC 2 cut(s) 249, 442
SaqAI TTAA 2 cut(s) 72, 680
SatI GCNGC 5 cut(s) 468, 715, 937, 940, 1100
Sau3AI GATC 7 cut(s) 55, 139, 389, 507, 822, 1121, 1161
Sau96I GGNCC 3 cut(s) 665, 854, 948
ScaI AGTACT 1 cut(s) 973
SduI GDGCHC 4 cut(s) 133, 559, 701, 1023
SfaNI GCATC 7 cut(s) 374, 461, 750, 772, 837, 852, 864
SfcI CTRYAG 2 cut(s) 465, 1097
Sfr303I CCGCGG 1 cut(s) 940
SgrBI CCGCGG 1 cut(s) 940
SinI GGWCC 2 cut(s) 854, 948
SmiMI CAYNNNNRTG 1 cut(s) 891
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 8 cut(s) 5, 77, 320, 415, 487, 583, 754, 1011
SsiI CCGC 4 cut(s) 715, 937, 939, 1102
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 701
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 1061
TaiI ACGT 2 cut(s) 163, 921
TaqI TCGA 6 cut(s) 252, 549, 741, 1047, 1124, 1131
TasI AATT 8 cut(s) 5, 77, 320, 415, 487, 583, 754, 1011
TatI WGTACW 2 cut(s) 273, 971
TauI GCSGC 3 cut(s) 717, 939, 942
TfiI GAWTC 3 cut(s) 738, 1067, 1193
Tru1I TTAA 2 cut(s) 72, 680
Tru9I TTAA 2 cut(s) 72, 680
TscAI CASTG 3 cut(s) 372, 992, 1119
TseFI GTSAC 3 cut(s) 150, 919, 993
TseI GCWGC 2 cut(s) 467, 1099
Tsp45I GTSAC 3 cut(s) 150, 919, 993
TspDTI ATGAA 5 cut(s) 17, 125, 417, 558, 1206
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 3 cut(s) 372, 992, 1119
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 2 cut(s) 854, 948
XapI RAATTY 3 cut(s) 415, 487, 754
XceI RCATGY 2 cut(s) 890, 1045
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 110
ZrmI AGTACT 1 cut(s) 973
Zsp2I ATGCAT 2 cut(s) 765, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.