Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004953

Physical Location & Seq

Genomic Coordinates Forward (+)

24453521 .. 24456548

Gene Structure 3028 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1135300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGAAATTCGAAGACTTTCTAATGCAGCGAAGCGAGGAGAAGCAGAAAAAACTCGACCTTGAGGAAGAGGTTAAGAAATTGCAGGCAGAGTTGGATGGAGAACAAACACTTGGCAGGTTTCTGCAATGTGCACTCCATGGACCTGTTCAGTCACATCCTTGTCTTTTATCGTTGTTTCCACCTCAGTTTCAGGAGCTGTTTTTGGAGCTGGCAATGGTGGAGGAAGAGATAGGTCAACTCGAAAGAAAAGTCGAAGAGCTGAAACTGAGGTTGTACCATGAGAGGGAACAAACAAGAGAGTGGGAAGTACAGCGGAAGCAATTAGGGCAACAGAATCACTTGTTATGCAGGGTGGGAAACCAGTCAGTGGTGAATGAAGAGAGATGCAGATCACAGAACTACGAAGCTTTGAGAAATTTGAGAAAAGAAAGAAGGTTGAAGGATAGAAGAGCTTCTGTAGGTTCTGCAGCAGATATCCCAAGATGGAATTTCACATATGCTGATGGTGAAACTGCTGAATTATCAAGGAGACAAAGGGGAAGAAGCAGAAACCAGGGCCATGGAGAAAATGATCTGAAACCAAATGAACTCTCGGAAGAACTTCTCAAGTGCCTTATATCCATATTTCTTGAACTGAAACAAACATCACTCGACAAGGAGGGATCATCTGTTGTCCCAAAGCTTGCACTTTCCTGCATGAACTCGAAGGGGCTCATTCCGAAGACCTCATTCAACTGCAAATCATCCACAATTTTCTTCAACTACAACACCTCGTCCAATGCCGACCCTTACTGCATATTGCCCGACTTAGACGGTGCTGTCAGGGATGTTGGCCCGTACAAAAACTTCATCCAGATCACGAGGAGCACGTTTGATGTCAGCCGATTGTCTGAGTGTTTGAATGCAATCGAAAAGTTGAGGAATTTGATGCATCAGCTGTGTGACGTGGACTTGACTTCCTTGACCTACAAGCAAAAGTTGGCGTTCTGGATCAACATCTACAACGCCTGCATCATGCATGCATTTCTGGAACATGGGCTGCCGTCCACACCGGAGAAACTGCTGGCACTTATGAACAAGGCTGCTCTAAATGTCGGAGGCATAGTGCTGAATGCTCTGGCTATCGAGCATTTCATTCTCCGCCATCCATCTGAATCGAAACATGGGCCTGCCTATGAGAAGGAAATGCTACTAAGACATGCGTACGGTCTCGGATACCCTGAACCTAACGTCACATTTGCTCTTTGCCGCGGCAGTTGGTCCTCACCGGCATTAAGGGTTTACACCTCCGACGACATAGTCAATGAGTTGGAGAGAGCAAAACTGGAGTACTTGGAAGCTTCGGTGGGAGTCACAAGCAAGAAGAAAATTGTGGTGCCCAAGCTCCTTCAATGGAACATGCTCGATTTTGCAGACGGTATGGAATCATTGCTGGAATGGATTTACAGCCAACTGCCGCGGTCTGGTTCGTTGAAACAATTGATAATGGAATGCTTGAATGGAGAGACGAAGTCTCCGATAATCAGAACGGTGGAAGTCCAGCCTTATGAATCCGAGTTCCGGTACCTGCTGCCGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF547 Lzipper-MIP1

Protein Analysis

527

Amino Acids

60.4

Weight (kDa)

6.5

Isoelectric Point (pI)

50.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lzipper-MIP1	PF14389	13 - 94	2.2e-17	Leucine-zipper of ternary complex factor MIP1
DUF547	PF04784	319 - 445	1.3e-38	Protein of unknown function, DUF547

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39690 AT2G39690 AT2G39690 AT2G39690 AT2G39690 AT2G39690 AT2G39690 AT2G39690 AT3G47675
fragaria_vesca	FvH4_7g22670 FvH4_7g22670
malus_domestica	MD01G1135300.v1.1 MD07G1200000.v1.1
prunus_persica	Prupe.2G236600_v2.0.a1 Prupe.2G236700_v2.0.a1 Prupe.2G236700_v2.0.a1
pyrus_communis	pycom01g15590 pycom01g15600 pycom07g18670 pycom07g18680
rosa_chinensis	RchiOBHm_Chr1g0367751
rosa_laevigata	RLG00000027266
rosa_multiflora	Rmu_ssc0000395.1_g000019
rosa_roxburghii	Rroxscaffold_4G00289260
rosa_rugosa	Rorug01G0336400
rosa_samantha	Rh1AG343500 Rh1BG305300 Rh1CG320900 Rh1DG337500
rosa_wichuraiana	Rw1G030570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	818, 1298
Acc36I	ACCTGC	2 cut(s)	105, 1575
Acc65I	GGTACC	1 cut(s)	1563
AccB1I	GGYRCC	2 cut(s)	1375, 1563
AccB7I	CCANNNNNTGG	1 cut(s)	367
AccII	CGCG	2 cut(s)	1251, 1459
AciI	CCGC	6 cut(s)	313, 1141, 1249, 1251, 1457, 1459
AclWI	GGATC	2 cut(s)	670, 998
AcsI	RAATTY	4 cut(s)	5, 415, 487, 922
AfaI	GTAC	6 cut(s)	275, 309, 839, 1205, 1331, 1565
AfiI	CCNNNNNNNGG	4 cut(s)	283, 367, 1463, 1560
AgsI	TTSAA	8 cut(s)	439, 632, 733, 760, 901, 1391, 1474, 1498
AjiI	CACGTC	1 cut(s)	946
AjnI	CCWGG	1 cut(s)	552
AjuI	GAANNNNNNNTTGG	2 cut(s)	93, 125
AluBI	AGCT	9 cut(s)	196, 208, 259, 407, 452, 682, 937, 1340, 1384
AluI	AGCT	9 cut(s)	196, 208, 259, 407, 452, 682, 937, 1340, 1384
Alw21I	GWGCWC	2 cut(s)	133, 869
Alw26I	GTCTC	4 cut(s)	523, 1214, 1499, 1518
Alw44I	GTGCAC	1 cut(s)	129
AlwI	GGATC	2 cut(s)	670, 998
AlwNI	CAGNNNCTG	1 cut(s)	196
AoxI	GGCC	3 cut(s)	556, 832, 1166
ApaLI	GTGCAC	1 cut(s)	129
ApeKI	GCWGC	5 cut(s)	25, 467, 1039, 1082, 1570
ApoI	RAATTY	4 cut(s)	5, 415, 487, 922
ArsI	GACNNNNNNTTYG	2 cut(s)	234, 266
Asp700I	GAANNNNTTC	3 cut(s)	15, 451, 600
Asp718I	GGTACC	1 cut(s)	1563
AspS9I	GGNCC	5 cut(s)	140, 556, 833, 1166, 1260
AsuHPI	GGTGA	3 cut(s)	382, 518, 1257
AsuII	TTCGAA	1 cut(s)	9
AvaII	GGWCC	2 cut(s)	140, 1260
BaeGI	GKGCMC	2 cut(s)	133, 1380
BanI	GGYRCC	2 cut(s)	1375, 1563
BanII	GRGCYC	1 cut(s)	714
BarI	GAAGNNNNNNTAC	2 cut(s)	830, 862
BauI	CACGAG	1 cut(s)	859
BbsI	GAAGAC	2 cut(s)	18, 728
Bbv12I	GWGCWC	2 cut(s)	133, 869
BbvI	GCAGC	5 cut(s)	37, 479, 1026, 1069, 1557
BccI	CCATC	5 cut(s)	89, 477, 497, 1152, 1156
BceAI	ACGGC	2 cut(s)	1027, 1558
BciT130I	CCWGG	1 cut(s)	554
BciVI	GTATCC	1 cut(s)	1208
BcoDI	GTCTC	4 cut(s)	523, 1214, 1499, 1518
BfmI	CTRYAG	2 cut(s)	456, 465
BfuAI	ACCTGC	2 cut(s)	105, 1575
BfuI	GTATCC	1 cut(s)	1208
BisI	GCNGC	8 cut(s)	26, 468, 1040, 1083, 1249, 1252, 1457, 1571
BlsI	GCNGC	8 cut(s)	27, 469, 1041, 1084, 1250, 1253, 1458, 1572
BmcAI	AGTACT	1 cut(s)	1331
Bme1390I	CCNGG	1 cut(s)	554
Bme18I	GGWCC	2 cut(s)	140, 1260
BmgBI	CACGTC	1 cut(s)	946
BmgT120I	GGNCC	5 cut(s)	140, 556, 833, 1166, 1260
BmiI	GGNNCC	2 cut(s)	1377, 1565
BmrFI	CCNGG	1 cut(s)	554
BmsI	GCATC	4 cut(s)	374, 918, 940, 1020
BpiI	GAAGAC	2 cut(s)	18, 728
BpmI	CTGGAG	1 cut(s)	1346
Bpu14I	TTCGAA	1 cut(s)	9
BpuEI	CTTGAG	2 cut(s)	80, 590
BsaBI	GATNNNNATC	2 cut(s)	388, 995
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	5 cut(s)	136, 553, 559, 1249, 1457
BsaWI	WCCGGW	2 cut(s)	1051, 1560
BsaXI	ACNNNNNCTCC	4 cut(s)	1089, 1119, 1498, 1528
Bsc4I	CCNNNNNNNGG	4 cut(s)	283, 367, 1463, 1560
Bse118I	RCCGGY	1 cut(s)	1267
Bse1I	ACTGG	2 cut(s)	361, 1329
Bse3DI	GCAATG	3 cut(s)	131, 219, 1427
Bse8I	GATNNNNATC	2 cut(s)	388, 995
BseBI	CCWGG	1 cut(s)	554
BseDI	CCNNGG	5 cut(s)	136, 553, 559, 1249, 1457
BseGI	GGATG	6 cut(s)	100, 154, 743, 832, 849, 1144
BseJI	GATNNNNATC	2 cut(s)	388, 995
BseLI	CCNNNNNNNGG	4 cut(s)	283, 367, 1463, 1560
BseMI	GCAATG	3 cut(s)	131, 219, 1427
BseMII	CTCAG	3 cut(s)	197, 257, 882
BseNI	ACTGG	2 cut(s)	361, 1329
BseRI	GAGGAG	2 cut(s)	50, 877
BseSI	GKGCMC	2 cut(s)	133, 1380
BseXI	GCAGC	5 cut(s)	37, 479, 1026, 1069, 1557
Bsh1236I	CGCG	2 cut(s)	1251, 1459
BshFI	GGCC	3 cut(s)	558, 834, 1168
BshNI	GGYRCC	2 cut(s)	1375, 1563
BsiHKAI	GWGCWC	2 cut(s)	133, 869
BsiSI	CCGG	3 cut(s)	1052, 1268, 1561
BsiWI	CGTACG	1 cut(s)	1203
BslFI	GGGAC	1 cut(s)	659
BslI	CCNNNNNNNGG	4 cut(s)	283, 367, 1463, 1560
BsmAI	GTCTC	4 cut(s)	523, 1214, 1499, 1518
BsmBI	CGTCTC	1 cut(s)	1499
BsmFI	GGGAC	1 cut(s)	659
BsmI	GAATGC	3 cut(s)	907, 1117, 1496
BsnI	GGCC	3 cut(s)	558, 834, 1168
Bso31I	GGTCTC	1 cut(s)	1214
Bsp119I	TTCGAA	1 cut(s)	9
Bsp1286I	GDGCHC	4 cut(s)	133, 714, 869, 1380
Bsp143I	GATC	5 cut(s)	389, 571, 662, 855, 990
Bsp19I	CCATGG	2 cut(s)	136, 559
BspACI	CCGC	6 cut(s)	313, 1141, 1249, 1251, 1457, 1459
BspANI	GGCC	3 cut(s)	558, 834, 1168
BspCNI	CTCAG	3 cut(s)	196, 258, 883
BspFNI	CGCG	2 cut(s)	1251, 1459
BspLI	GGNNCC	2 cut(s)	1377, 1565
BspMAI	CTGCAG	1 cut(s)	469
BspMI	ACCTGC	2 cut(s)	105, 1575
BspPI	GGATC	2 cut(s)	670, 998
BspQI	GCTCTTC	2 cut(s)	249, 442
BspT104I	TTCGAA	1 cut(s)	9
BspT107I	GGYRCC	2 cut(s)	1375, 1563
BspTNI	GGTCTC	1 cut(s)	1214
BsrDI	GCAATG	3 cut(s)	131, 219, 1427
BsrFI	RCCGGY	1 cut(s)	1267
BsrI	ACTGG	2 cut(s)	361, 1329
BssAI	RCCGGY	1 cut(s)	1267
BssECI	CCNNGG	5 cut(s)	136, 553, 559, 1249, 1457
BssMI	GATC	5 cut(s)	389, 571, 662, 855, 990
BssSI	CACGAG	1 cut(s)	859
BssT1I	CCWWGG	2 cut(s)	136, 559
Bst2BI	CACGAG	1 cut(s)	859
Bst2UI	CCWGG	1 cut(s)	554
Bst4CI	ACNGT	4 cut(s)	815, 1208, 1418, 1531
Bst6I	CTCTTC	5 cut(s)	60, 219, 249, 372, 442
BstBI	TTCGAA	1 cut(s)	9
BstC8I	GCNNGC	7 cut(s)	84, 210, 684, 1009, 1020, 1065, 1170
BstDEI	CTNAG	5 cut(s)	183, 266, 808, 891, 1193
BstDSI	CCRYGG	4 cut(s)	136, 559, 1249, 1457
BstF5I	GGATG	6 cut(s)	100, 154, 743, 832, 849, 1144
BstFNI	CGCG	2 cut(s)	1251, 1459
BstKTI	GATC	5 cut(s)	392, 574, 665, 858, 993
BstMAI	GTCTC	4 cut(s)	523, 1214, 1499, 1518
BstMBI	GATC	5 cut(s)	389, 571, 662, 855, 990
BstMWI	GCNNNNNNNGC	1 cut(s)	325
BstNI	CCWGG	1 cut(s)	554
BstNSI	RCATGY	3 cut(s)	1022, 1202, 1402
BstSCI	CCNGG	1 cut(s)	552
BstSFI	CTRYAG	2 cut(s)	456, 465
BstSLI	GKGCMC	2 cut(s)	133, 1380
BstUI	CGCG	2 cut(s)	1251, 1459
BstV1I	GCAGC	5 cut(s)	37, 479, 1026, 1069, 1557
BstV2I	GAAGAC	2 cut(s)	18, 728
BstXI	CCANNNNNNTGG	1 cut(s)	560
BsuI	GTATCC	1 cut(s)	1208
BsuRI	GGCC	3 cut(s)	558, 834, 1168
BtgI	CCRYGG	4 cut(s)	136, 559, 1249, 1457
BtrI	CACGTC	1 cut(s)	946
BtsCI	GGATG	6 cut(s)	100, 154, 743, 832, 849, 1144
BtsIMutI	CAGTG	1 cut(s)	372
BveI	ACCTGC	2 cut(s)	105, 1575
Cac8I	GCNNGC	7 cut(s)	84, 210, 684, 1009, 1020, 1065, 1170
CaiI	CAGNNNCTG	1 cut(s)	196
Cfr10I	RCCGGY	1 cut(s)	1267
Cfr13I	GGNCC	5 cut(s)	140, 556, 833, 1166, 1260
Cfr42I	CCGCGG	2 cut(s)	1252, 1460
Csp6I	GTAC	6 cut(s)	274, 308, 838, 1204, 1330, 1564
CviQI	GTAC	6 cut(s)	274, 308, 838, 1204, 1330, 1564
DdeI	CTNAG	5 cut(s)	183, 266, 808, 891, 1193
DpnI	GATC	5 cut(s)	391, 573, 664, 857, 992
DpnII	GATC	5 cut(s)	389, 571, 662, 855, 990
DrdI	GACNNNNNNGTC	2 cut(s)	818, 1298
DseDI	GACNNNNNNGTC	2 cut(s)	818, 1298
Eam1104I	CTCTTC	5 cut(s)	60, 219, 249, 372, 442
EarI	CTCTTC	5 cut(s)	60, 219, 249, 372, 442
EciI	GGCGGA	1 cut(s)	1130
Eco130I	CCWWGG	2 cut(s)	136, 559
Eco24I	GRGCYC	1 cut(s)	714
Eco31I	GGTCTC	1 cut(s)	1214
Eco32I	GATATC	1 cut(s)	475
Eco47I	GGWCC	2 cut(s)	140, 1260
EcoRII	CCWGG	1 cut(s)	552
EcoRV	GATATC	1 cut(s)	475
EcoT14I	CCWWGG	2 cut(s)	136, 559
EcoT22I	ATGCAT	3 cut(s)	933, 1020, 1024
EcoT38I	GRGCYC	1 cut(s)	714
ErhI	CCWWGG	2 cut(s)	136, 559
Esp3I	CGTCTC	1 cut(s)	1499
FaqI	GGGAC	1 cut(s)	659
FauNDI	CATATG	1 cut(s)	496
Fnu4HI	GCNGC	8 cut(s)	26, 468, 1040, 1083, 1249, 1252, 1457, 1571
FokI	GGATG	6 cut(s)	107, 141, 730, 836, 839, 1131
FriOI	GRGCYC	1 cut(s)	714
Fsp4HI	GCNGC	8 cut(s)	26, 468, 1040, 1083, 1249, 1252, 1457, 1571
GluI	GCNGC	8 cut(s)	26, 468, 1040, 1083, 1249, 1252, 1457, 1571
GsuI	CTGGAG	1 cut(s)	1346
HaeIII	GGCC	3 cut(s)	558, 834, 1168
HapII	CCGG	3 cut(s)	1052, 1268, 1561
HincII	GTYRAC	1 cut(s)	236
HindII	GTYRAC	1 cut(s)	236
HindIII	AAGCTT	3 cut(s)	405, 680, 1338
HinfI	GANTC	5 cut(s)	334, 1154, 1350, 1424, 1550
HpaII	CCGG	3 cut(s)	1052, 1268, 1561
HphI	GGTGA	3 cut(s)	382, 518, 1257
Hpy166II	GTNNAC	5 cut(s)	131, 236, 949, 1047, 1282
Hpy188III	TCNNGA	6 cut(s)	191, 629, 853, 859, 988, 1028
Hpy8I	GTNNAC	5 cut(s)	131, 236, 949, 1047, 1282
Hpy99I	CGWCG	1 cut(s)	1295
HpyAV	CCTTC	5 cut(s)	426, 433, 700, 1174, 1397
HpyCH4III	ACNGT	4 cut(s)	815, 1208, 1418, 1531
HpyCH4IV	ACGT	3 cut(s)	869, 945, 1230
HpyF10VI	GCNNNNNNNGC	1 cut(s)	325
HpyF3I	CTNAG	5 cut(s)	183, 266, 808, 891, 1193
HpySE526I	ACGT	3 cut(s)	869, 945, 1230
KpnI	GGTACC	1 cut(s)	1567
KspI	CCGCGG	2 cut(s)	1252, 1460
Kzo9I	GATC	5 cut(s)	389, 571, 662, 855, 990
LguI	GCTCTTC	2 cut(s)	249, 442
LmnI	GCTCC	4 cut(s)	193, 205, 864, 1389
Lsp1109I	GCAGC	5 cut(s)	37, 479, 1026, 1069, 1557
LweI	GCATC	4 cut(s)	374, 918, 940, 1020
MaeII	ACGT	3 cut(s)	869, 945, 1230
MaeIII	GTNAC	4 cut(s)	150, 941, 1231, 1351
MalI	GATC	5 cut(s)	391, 573, 664, 857, 992
MboI	GATC	5 cut(s)	389, 571, 662, 855, 990
MfeI	CAATTG	1 cut(s)	1478
MhlI	GDGCHC	4 cut(s)	133, 714, 869, 1380
MlyI	GAGTC	1 cut(s)	1359
MmeI	TCCRAC	4 cut(s)	72, 1075, 1290, 1314
Mph1103I	ATGCAT	3 cut(s)	933, 1020, 1024
MroXI	GAANNNNTTC	3 cut(s)	15, 451, 600
MseI	TTAA	2 cut(s)	72, 1274
MspA1I	CMGCKG	4 cut(s)	313, 937, 1251, 1459
MspI	CCGG	3 cut(s)	1052, 1268, 1561
MspR9I	CCNGG	1 cut(s)	554
MunI	CAATTG	1 cut(s)	1478
Mva1269I	GAATGC	3 cut(s)	907, 1117, 1496
MvaI	CCWGG	1 cut(s)	554
MvnI	CGCG	2 cut(s)	1251, 1459
MwoI	GCNNNNNNNGC	1 cut(s)	325
NcoI	CCATGG	2 cut(s)	136, 559
NdeI	CATATG	1 cut(s)	496
NdeII	GATC	5 cut(s)	389, 571, 662, 855, 990
NlaIV	GGNNCC	2 cut(s)	1377, 1565
NmuCI	GTSAC	4 cut(s)	150, 941, 1231, 1351
NsiI	ATGCAT	3 cut(s)	933, 1020, 1024
NspI	RCATGY	3 cut(s)	1022, 1202, 1402
NspV	TTCGAA	1 cut(s)	9
PaeI	GCATGC	1 cut(s)	1022
PciSI	GCTCTTC	2 cut(s)	249, 442
PcsI	WCGNNNNNNNCGW	2 cut(s)	866, 1514
PctI	GAATGC	3 cut(s)	907, 1117, 1496
PdmI	GAANNNNTTC	3 cut(s)	15, 451, 600
PfeI	GAWTC	4 cut(s)	334, 1154, 1424, 1550
Pfl23II	CGTACG	1 cut(s)	1203
PflFI	GACNNNGTC	2 cut(s)	1298, 1510
PflMI	CCANNNNNTGG	1 cut(s)	367
PkrI	GCNGC	8 cut(s)	27, 469, 1041, 1084, 1250, 1253, 1458, 1572
PleI	GAGTC	1 cut(s)	1358
PpsI	GAGTC	1 cut(s)	1358
Psp6I	CCWGG	1 cut(s)	552
PspGI	CCWGG	1 cut(s)	552
PspLI	CGTACG	1 cut(s)	1203
PspN4I	GGNNCC	2 cut(s)	1377, 1565
PspPI	GGNCC	5 cut(s)	140, 556, 833, 1166, 1260
PstI	CTGCAG	1 cut(s)	469
PstNI	CAGNNNCTG	1 cut(s)	196
PsyI	GACNNNGTC	2 cut(s)	1298, 1510
PvuII	CAGCTG	1 cut(s)	937
RsaI	GTAC	6 cut(s)	275, 309, 839, 1205, 1331, 1565
RsaNI	GTAC	6 cut(s)	274, 308, 838, 1204, 1330, 1564
SacII	CCGCGG	2 cut(s)	1252, 1460
SapI	GCTCTTC	2 cut(s)	249, 442
SaqAI	TTAA	2 cut(s)	72, 1274
SatI	GCNGC	8 cut(s)	26, 468, 1040, 1083, 1249, 1252, 1457, 1571
Sau3AI	GATC	5 cut(s)	389, 571, 662, 855, 990
Sau96I	GGNCC	5 cut(s)	140, 556, 833, 1166, 1260
ScaI	AGTACT	1 cut(s)	1331
SchI	GAGTC	1 cut(s)	1359
ScrFI	CCNGG	1 cut(s)	554
SduI	GDGCHC	4 cut(s)	133, 714, 869, 1380
SfaNI	GCATC	4 cut(s)	374, 918, 940, 1020
SfcI	CTRYAG	2 cut(s)	456, 465
Sfr303I	CCGCGG	2 cut(s)	1252, 1460
SfuI	TTCGAA	1 cut(s)	9
SgrBI	CCGCGG	2 cut(s)	1252, 1460
SinI	GGWCC	2 cut(s)	140, 1260
SmlI	CTYRAG	2 cut(s)	59, 605
SmoI	CTYRAG	2 cut(s)	59, 605
SphI	GCATGC	1 cut(s)	1022
SsiI	CCGC	6 cut(s)	313, 1141, 1249, 1251, 1457, 1459
StyD4I	CCNGG	1 cut(s)	552
StyI	CCWWGG	2 cut(s)	136, 559
TaaI	ACNGT	4 cut(s)	815, 1208, 1418, 1531
TaiI	ACGT	3 cut(s)	872, 948, 1233
TatI	WGTACW	2 cut(s)	307, 1329
TauI	GCSGC	3 cut(s)	1251, 1254, 1459
TfiI	GAWTC	4 cut(s)	334, 1154, 1424, 1550
Tru1I	TTAA	2 cut(s)	72, 1274
Tru9I	TTAA	2 cut(s)	72, 1274
TscAI	CASTG	1 cut(s)	372
TseFI	GTSAC	4 cut(s)	150, 941, 1231, 1351
TseI	GCWGC	5 cut(s)	25, 467, 1039, 1082, 1570
Tsp45I	GTSAC	4 cut(s)	150, 941, 1231, 1351
TspDTI	ATGAA	8 cut(s)	17, 390, 600, 713, 838, 1088, 1123, 1563
TspRI	CASTG	1 cut(s)	372
Tth111I	GACNNNGTC	2 cut(s)	1298, 1510
Van91I	CCANNNNNTGG	1 cut(s)	367
VneI	GTGCAC	1 cut(s)	129
VpaK11BI	GGWCC	2 cut(s)	140, 1260
XapI	RAATTY	4 cut(s)	5, 415, 487, 922
XceI	RCATGY	3 cut(s)	1022, 1202, 1402
XmnI	GAANNNNTTC	3 cut(s)	15, 451, 600
ZrmI	AGTACT	1 cut(s)	1331
Zsp2I	ATGCAT	3 cut(s)	933, 1020, 1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.