FvH4_7g26020
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010870
Physical Location & Seq
Forward (+)
19780987 .. 19783467
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26020.t1

Sequence Viewer

Length: 1140 bp
ATGGAGCACCAGCACAGAAGCCTCACCACAGCTTACATTAGCTACCCTTTTAAATTTCACCACAACATTTTCAAAAACACGACAAGCCAAACCAAGAAAACCATGGATGACTTTAGAAGTGAAACCGTTTCTATAATTCACCATGGTTGCAAATTGGCTAGAGACCTGGAATCAAACCTTCCCAACTTGGCAAACCAACCCGAAACCGTTTCGAAATCATTGGAAGAGATCATTAGGGTTTTTGGTGCAGCGAGGGAGAGGCTGCAGAGTAGTAGTGCTGCACAACAAGACCCGATGAGTTCATACGTTCATGTAGCACAGCAGCAGCAGCGGTTCGATGCGAGCTTGCAGGAATGGCTGAGCAGGTGTAATTACACACAGGCAATGGAGGATCATTACATCCAAACGCAGCGTTTCGCAGCCGAAAAGAGTAGTGCTGCGGATTCAGGGGCTGAAGCACTTCTTCAAGGCCAAATACATCATGCAATGGATCATATTGTAACAGTGATTTCTCATGAAATGAATGCTGCTTCGTCCTCACAAAGCAGACCACGAAGAAGGAGGGATCATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCGGATCGGGAATACTGAAATCCCGCCGGAGGACGGCTTCACTTGGCGAAAATACGGCCAGAAGGAGATAATGGGATGCAGGTTCCCGAGGGGCTATTATAGGTGCACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATGTACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAGTTCTGCCAGCATCTGATGATCAAATTAAGCAAGAGACTATGACCCAAACTCATGCAGCAACCACTACTCAACCCTTAGGAACATGGCTCTCCATGGACTTCAACGCAGGAGGCGAAGGAAGCAGTGGTGGCGGCGGCCGTGGTGACGCTGATGGTTTAGCTACGTCAACCACGACCACGACCACGCGTTATGGTAAAGATGTTGAGTTTCCGGTGGTGGATTTGGCTGACGCAATGTTTAATTCAGGGAGTAGCAGCAGTAATAGTATGGATTTTATGTTCACTTCACCAATGGACAATAAATAG

Protein Analysis

380

Amino Acids

42.42

Weight (kDa)

6.63

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 211 - 271 3.3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 354
Acc36I ACCTGC 2 cut(s) 354, 672
AccB1I GGYRCC 1 cut(s) 598
AccII CGCG 1 cut(s) 1021
AciI CCGC 5 cut(s) 331, 440, 626, 966, 969
AclWI GGATC 5 cut(s) 399, 498, 573, 614, 761
AcoI YGGCCR 2 cut(s) 658, 970
AcsI RAATTY 2 cut(s) 53, 576
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 3 cut(s) 608, 631, 635
AflIII ACRYGT 2 cut(s) 806, 1019
AgsI TTSAA 4 cut(s) 73, 467, 782, 937
AjnI CCWGG 1 cut(s) 165
AloI GAACNNNNNNTCC 2 cut(s) 1097, 1129
AluBI AGCT 4 cut(s) 32, 42, 345, 995
AluI AGCT 4 cut(s) 32, 42, 345, 995
Alw21I GWGCWC 2 cut(s) 9, 710
Alw26I GTCTC 2 cut(s) 156, 863
Alw44I GTGCAC 1 cut(s) 706
AlwI GGATC 5 cut(s) 399, 498, 573, 614, 761
AlwNI CAGNNNCTG 2 cut(s) 452, 848
Ama87I CYCGRG 1 cut(s) 688
AoxI GGCC 3 cut(s) 469, 658, 970
ApaLI GTGCAC 1 cut(s) 706
ApoI RAATTY 2 cut(s) 53, 576
Asp700I GAANNNNTTC 1 cut(s) 459
AsuHPI GGTGA 6 cut(s) 16, 50, 131, 809, 989, 1113
AsuII TTCGAA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 688
AxyI CCTNAGG 1 cut(s) 910
BaeGI GKGCMC 1 cut(s) 710
BanI GGYRCC 1 cut(s) 598
Bbv12I GWGCWC 2 cut(s) 9, 710
BccI CCATC 1 cut(s) 980
BceAI ACGGC 3 cut(s) 652, 673, 957
BciT130I CCWGG 1 cut(s) 167
BclI TGATCA 1 cut(s) 853
BcoDI GTCTC 2 cut(s) 156, 863
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 354, 672
BlpI GCTNAGC 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 688
BmiI GGNNCC 2 cut(s) 600, 686
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 3 cut(s) 328, 668, 854
Bpu1102I GCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 212
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 6 cut(s) 102, 142, 602, 689, 927, 973
BsaWI WCCGGW 1 cut(s) 1045
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 631, 635
Bse118I RCCGGY 1 cut(s) 595
Bse21I CCTNAGG 1 cut(s) 910
Bse3DI GCAATG 3 cut(s) 390, 492, 1074
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 6 cut(s) 102, 142, 602, 689, 927, 973
BseGI GGATG 3 cut(s) 112, 399, 683
BseLI CCNNNNNNNGG 3 cut(s) 608, 631, 635
BseMI GCAATG 3 cut(s) 390, 492, 1074
BseMII CTCAG 2 cut(s) 350, 846
BseSI GKGCMC 1 cut(s) 710
BseX3I CGGCCG 1 cut(s) 970
BseYI CCCAGC 1 cut(s) 733
BsgI GTGCAG 3 cut(s) 264, 267, 810
Bsh1236I CGCG 1 cut(s) 1021
Bsh1285I CGRYCG 1 cut(s) 973
BshFI GGCC 3 cut(s) 471, 660, 972
BshNI GGYRCC 1 cut(s) 598
BsiEI CGRYCG 1 cut(s) 973
BsiHKAI GWGCWC 2 cut(s) 9, 710
BsiHKCI CYCGRG 1 cut(s) 688
BsiSI CCGG 3 cut(s) 596, 629, 1046
BslI CCNNNNNNNGG 3 cut(s) 608, 631, 635
BsmAI GTCTC 2 cut(s) 156, 863
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 3 cut(s) 471, 660, 972
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 688
Bsp119I TTCGAA 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 9, 710
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
Bsp1720I GCTNAGC 1 cut(s) 359
Bsp19I CCATGG 3 cut(s) 102, 142, 927
BspACI CCGC 5 cut(s) 331, 440, 626, 966, 969
BspANI GGCC 3 cut(s) 471, 660, 972
BspCNI CTCAG 2 cut(s) 351, 845
BspFNI CGCG 1 cut(s) 1021
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 600, 686
BspMAI CTGCAG 1 cut(s) 267
BspMI ACCTGC 2 cut(s) 354, 672
BspPI GGATC 5 cut(s) 399, 498, 573, 614, 761
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 3 cut(s) 390, 492, 1074
BsrFI RCCGGY 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 789
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 6 cut(s) 102, 142, 602, 689, 927, 973
BssMI GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
BssT1I CCWWGG 3 cut(s) 102, 142, 927
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 4 cut(s) 127, 208, 505, 592
Bst6I CTCTTC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 789
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 4 cut(s) 343, 347, 597, 843
BstDEI CTNAG 3 cut(s) 359, 832, 910
BstDSI CCRYGG 4 cut(s) 102, 142, 927, 973
BstEII GGTNACC 1 cut(s) 797
BstF5I GGATG 3 cut(s) 112, 399, 683
BstFNI CGCG 1 cut(s) 1021
BstKTI GATC 7 cut(s) 231, 394, 493, 568, 609, 769, 856
BstMAI GTCTC 2 cut(s) 156, 863
BstMBI GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
BstMCI CGRYCG 1 cut(s) 973
BstMWI GCNNNNNNNGC 4 cut(s) 328, 355, 954, 963
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 810
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 263
BstSLI GKGCMC 1 cut(s) 710
BstUI CGCG 1 cut(s) 1021
BstZI CGGCCG 1 cut(s) 970
Bsu36I CCTNAGG 1 cut(s) 910
BsuRI GGCC 3 cut(s) 471, 660, 972
BtgI CCRYGG 4 cut(s) 102, 142, 927, 973
BtsCI GGATG 3 cut(s) 112, 399, 683
BtsI GCAGTG 2 cut(s) 822, 964
BtsIMutI CAGTG 3 cut(s) 510, 822, 964
BveI ACCTGC 2 cut(s) 354, 672
Cac8I GCNNGC 4 cut(s) 343, 347, 597, 843
CaiI CAGNNNCTG 2 cut(s) 452, 848
CciI TCATGA 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 595
CseI GACGC 2 cut(s) 989, 1073
Csp6I GTAC 1 cut(s) 790
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 3 cut(s) 359, 832, 910
DpnI GATC 7 cut(s) 230, 393, 492, 567, 608, 768, 855
DpnII GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 2 cut(s) 658, 970
EagI CGGCCG 1 cut(s) 970
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EclXI CGGCCG 1 cut(s) 970
Eco130I CCWWGG 3 cut(s) 102, 142, 927
Eco31I GGTCTC 1 cut(s) 156
Eco52I CGGCCG 1 cut(s) 970
Eco57I CTGAAG 1 cut(s) 474
Eco81I CCTNAGG 1 cut(s) 910
Eco88I CYCGRG 1 cut(s) 688
Eco91I GGTNACC 1 cut(s) 797
EcoO65I GGTNACC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 102, 142, 927
ErhI CCWWGG 3 cut(s) 102, 142, 927
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FauI CCCGC 1 cut(s) 633
FauNDI CATATG 1 cut(s) 813
FbaI TGATCA 1 cut(s) 853
FokI GGATG 3 cut(s) 119, 386, 690
FspBI CTAG 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 737
HaeIII GGCC 3 cut(s) 471, 660, 972
HapII CCGG 3 cut(s) 596, 629, 1046
HgaI GACGC 2 cut(s) 989, 1073
HincII GTYRAC 1 cut(s) 1002
HindII GTYRAC 1 cut(s) 1002
HinfI GANTC 2 cut(s) 170, 443
HpaII CCGG 3 cut(s) 596, 629, 1046
HphI GGTGA 6 cut(s) 16, 50, 131, 809, 989, 1113
Hpy166II GTNNAC 4 cut(s) 708, 822, 1002, 1116
Hpy188I TCNGA 3 cut(s) 606, 753, 850
Hpy188III TCNNGA 3 cut(s) 515, 610, 688
Hpy8I GTNNAC 4 cut(s) 708, 822, 1002, 1116
HpyAV CCTTC 4 cut(s) 188, 552, 658, 944
HpyCH4III ACNGT 4 cut(s) 127, 208, 505, 592
HpyCH4IV ACGT 2 cut(s) 306, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 328, 355, 954, 963
HpyF3I CTNAG 3 cut(s) 359, 832, 910
HpySE526I ACGT 2 cut(s) 306, 998
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
LmnI GCTCC 1 cut(s) 4
LweI GCATC 3 cut(s) 328, 668, 854
MaeI CTAG 1 cut(s) 159
MaeII ACGT 2 cut(s) 306, 998
MaeIII GTNAC 3 cut(s) 499, 797, 977
MalI GATC 7 cut(s) 230, 393, 492, 567, 608, 768, 855
MboI GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
MboII GAAGA 3 cut(s) 236, 455, 567
MfeI CAATTG 1 cut(s) 727
MhlI GDGCHC 2 cut(s) 9, 710
MluCI AATT 9 cut(s) 53, 135, 152, 370, 576, 584, 727, 858, 1075
MluI ACGCGT 1 cut(s) 1019
MroNI GCCGGC 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 4 cut(s) 51, 773, 861, 1074
MspA1I CMGCKG 1 cut(s) 331
MspI CCGG 3 cut(s) 596, 629, 1046
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 727
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 1021
MwoI GCNNNNNNNGC 4 cut(s) 328, 355, 954, 963
NaeI GCCGGC 1 cut(s) 597
NcoI CCATGG 3 cut(s) 102, 142, 927
NdeI CATATG 1 cut(s) 813
NdeII GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
NgoMIV GCCGGC 1 cut(s) 595
NlaIV GGNNCC 2 cut(s) 600, 686
NmuCI GTSAC 2 cut(s) 797, 977
NspI RCATGY 1 cut(s) 810
NspV TTCGAA 1 cut(s) 212
PagI TCATGA 1 cut(s) 514
PaqCI CACCTGC 1 cut(s) 354
PciI ACATGT 1 cut(s) 806
PcsI WCGNNNNNNNCGW 2 cut(s) 945, 1004
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 597
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 170, 443
PscI ACATGT 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 165
PspEI GGTNACC 1 cut(s) 797
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 600, 686
PstI CTGCAG 1 cut(s) 267
PstNI CAGNNNCTG 2 cut(s) 452, 848
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
SaqAI TTAA 4 cut(s) 51, 773, 861, 1074
Sau3AI GATC 7 cut(s) 228, 391, 490, 565, 606, 766, 853
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 2 cut(s) 9, 710
SfaNI GCATC 3 cut(s) 328, 668, 854
SfcI CTRYAG 1 cut(s) 263
SfuI TTCGAA 1 cut(s) 212
Sse9I AATT 9 cut(s) 53, 135, 152, 370, 576, 584, 727, 858, 1075
SsiI CCGC 5 cut(s) 331, 440, 626, 966, 969
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 3 cut(s) 102, 142, 927
TaaI ACNGT 4 cut(s) 127, 208, 505, 592
TaiI ACGT 2 cut(s) 309, 1001
TaqI TCGA 2 cut(s) 212, 336
TasI AATT 9 cut(s) 53, 135, 152, 370, 576, 584, 727, 858, 1075
TatI WGTACW 1 cut(s) 789
TauI GCSGC 2 cut(s) 969, 972
TfiI GAWTC 2 cut(s) 170, 443
Tru1I TTAA 4 cut(s) 51, 773, 861, 1074
Tru9I TTAA 4 cut(s) 51, 773, 861, 1074
TscAI CASTG 3 cut(s) 510, 829, 964
TseFI GTSAC 2 cut(s) 797, 977
Tsp45I GTSAC 2 cut(s) 797, 977
TspDTI ATGAA 4 cut(s) 291, 299, 531, 536
TspRI CASTG 3 cut(s) 510, 829, 964
VneI GTGCAC 1 cut(s) 706
XapI RAATTY 2 cut(s) 53, 576
XceI RCATGY 1 cut(s) 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.