RLG00000026899
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010870
Physical Location & Seq
Reverse (-)
6849988 .. 6852161
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026899

Sequence Viewer

Length: 1071 bp
ATGGATAACATTAGTGAAACCGTTTCTTTAATTCACCGAGGTTGCAACTTGGCTAGAGACCTGGAATCAAACCTTCCCAACTTGGCAAGCCGACCCGAAACCGTTTCGAAATCCTTGGACGAGATAATGAGGGTTTTCGGTGCAGCGAGGGAGAGGCTGCAGAGTAGTAGTGCTGCACAACATGATCCAATGAGTTCATACGTTCATGTAGCGCAGCAGCAGCAGCAGATTGATGCGAGCTTGCAGGAATTGCTGAGGTCTAATATTTACACACAGGCAATGGAGGATCATTACATCCAAATTCAGCGTTTTACATCCGAAACTAGTAGTGCTGCTACTCAATCCGATATGAGGGATCCAGGAGCTGACGTACTTCAAGGCCAAATCCATCAGGCAATGGATCATATTGTAACAGTCGTTTCTCCTGAACTGAATGCTGCTTCGTCCTCACAAAGCAGACCACGAAGAAGGAGGGACCATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCGGATCGGGAATACTGAAATCCCACCGGAGGATGGCTTCACTTGGCGAAAATACGGCCAGAAGGAAATCATGGGCTGCAGGTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATGTACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAAGTCTACCAGCATCTGATCAAATTAAGCAAGAGACTATGACCCAAACTCATGCGGCAACCACTCAACCCTTAGGAACATGGCTCTCCATGGACTTCAACGCAAGAGGCGGAGGAAGCAGCAGTGGTGGTGGCCGCGGTGATGGTGATGGTGATGGTGTAGGTACATCAATCACGACGACCACTCGTTATGGTAAAGACGTTGAGTTTCCGGTGTTGGATTTGGCTGATGCAATGTTTAATTCAGGGAGTAGCAGCAGTAATAGTATGGATTTTATGTTCACTTCATCTATGGACAGTAAATGGGAGTCCGGGGACAAGAAAAATTGA

Protein Analysis

357

Amino Acids

39.38

Weight (kDa)

6.0

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 181 - 241 3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 748
AccII CGCG 1 cut(s) 879
AciI CCGC 4 cut(s) 797, 852, 877, 879
AclWI GGATC 7 cut(s) 179, 294, 350, 363, 408, 524, 671
AcoI YGGCCR 2 cut(s) 568, 874
AcsI RAATTY 2 cut(s) 300, 486
AfaI GTAC 4 cut(s) 372, 619, 701, 907
AfiI CCNNNNNNNGG 4 cut(s) 351, 518, 541, 545
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 3 cut(s) 377, 692, 841
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 2 cut(s) 60, 358
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
AluBI AGCT 2 cut(s) 240, 365
AluI AGCT 2 cut(s) 240, 365
Alw26I GTCTC 2 cut(s) 51, 770
AlwI GGATC 7 cut(s) 179, 294, 350, 363, 408, 524, 671
AlwNI CAGNNNCTG 2 cut(s) 365, 758
AoxI GGCC 3 cut(s) 379, 568, 874
ApoI RAATTY 2 cut(s) 300, 486
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 475
AsuC2I CCSGG 1 cut(s) 1054
AsuHPI GGTGA 5 cut(s) 26, 719, 893, 899, 905
AsuII TTCGAA 1 cut(s) 107
AvaII GGWCC 1 cut(s) 475
AxyI CCTNAGG 1 cut(s) 814
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 508
BbvCI CCTCAGC 1 cut(s) 254
BccI CCATC 5 cut(s) 396, 539, 878, 884, 890
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 2 cut(s) 62, 360
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 1 cut(s) 1054
BcoDI GTCTC 2 cut(s) 51, 770
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 2 cut(s) 54, 324
BfmI CTRYAG 3 cut(s) 158, 589, 610
BfuAI ACCTGC 1 cut(s) 582
Bme1390I CCNGG 3 cut(s) 62, 360, 1054
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 4 cut(s) 357, 476, 510, 596
BmrFI CCNGG 3 cut(s) 62, 360, 1054
BmsI GCATC 3 cut(s) 223, 764, 961
Bpu10I CCTNAGC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 727
BpuMI CCSGG 1 cut(s) 1054
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 8 cut(s) 37, 114, 478, 512, 599, 831, 877, 1053
BsaWI WCCGGW 2 cut(s) 538, 952
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 4 cut(s) 351, 518, 541, 545
Bse118I RCCGGY 1 cut(s) 505
Bse21I CCTNAGG 1 cut(s) 814
Bse3DI GCAATG 3 cut(s) 285, 402, 981
BseBI CCWGG 2 cut(s) 62, 360
BseDI CCNNGG 8 cut(s) 37, 114, 478, 512, 599, 831, 877, 1053
BseGI GGATG 3 cut(s) 294, 314, 550
BseLI CCNNNNNNNGG 4 cut(s) 351, 518, 541, 545
BseMI GCAATG 3 cut(s) 285, 402, 981
BseMII CTCAG 1 cut(s) 245
BseYI CCCAGC 1 cut(s) 643
BsgI GTGCAG 3 cut(s) 159, 162, 720
Bsh1236I CGCG 1 cut(s) 879
BshFI GGCC 3 cut(s) 381, 570, 876
BshNI GGYRCC 1 cut(s) 508
BsiSI CCGG 4 cut(s) 506, 539, 953, 1053
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 4 cut(s) 351, 518, 541, 545
BsmAI GTCTC 2 cut(s) 51, 770
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 3 cut(s) 381, 570, 876
Bso31I GGTCTC 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
Bsp19I CCATGG 2 cut(s) 478, 831
BspACI CCGC 4 cut(s) 797, 852, 877, 879
BspANI GGCC 3 cut(s) 381, 570, 876
BspCNI CTCAG 1 cut(s) 246
BspFNI CGCG 1 cut(s) 879
BspLI GGNNCC 4 cut(s) 357, 476, 510, 596
BspMAI CTGCAG 2 cut(s) 162, 593
BspMI ACCTGC 1 cut(s) 582
BspPI GGATC 7 cut(s) 179, 294, 350, 363, 408, 524, 671
BspT104I TTCGAA 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 3 cut(s) 285, 402, 981
BsrFI RCCGGY 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 699
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 8 cut(s) 37, 114, 478, 512, 599, 831, 877, 1053
BssMI GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
BssT1I CCWWGG 4 cut(s) 114, 478, 599, 831
Bst2UI CCWGG 2 cut(s) 62, 360
Bst4CI ACNGT 5 cut(s) 22, 103, 415, 502, 1040
BstAPI GCANNNNNTGC 1 cut(s) 250
BstAUI TGTACA 1 cut(s) 699
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 4 cut(s) 88, 238, 242, 507
BstDEI CTNAG 2 cut(s) 254, 814
BstDSI CCRYGG 3 cut(s) 478, 831, 877
BstEII GGTNACC 1 cut(s) 707
BstF5I GGATG 3 cut(s) 294, 314, 550
BstFNI CGCG 1 cut(s) 879
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 7 cut(s) 187, 289, 358, 403, 519, 679, 763
BstMAI GTCTC 2 cut(s) 51, 770
BstMBI GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
BstMWI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 858
BstNI CCWGG 2 cut(s) 62, 360
BstNSI RCATGY 1 cut(s) 720
BstPI GGTNACC 1 cut(s) 707
BstSCI CCNGG 3 cut(s) 60, 358, 1052
BstSFI CTRYAG 3 cut(s) 158, 589, 610
BstUI CGCG 1 cut(s) 879
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu36I CCTNAGG 1 cut(s) 814
BsuRI GGCC 3 cut(s) 381, 570, 876
BtgI CCRYGG 3 cut(s) 478, 831, 877
BtsCI GGATG 3 cut(s) 294, 314, 550
BtsI GCAGTG 2 cut(s) 732, 871
BtsIMutI CAGTG 2 cut(s) 732, 871
BveI ACCTGC 1 cut(s) 582
Cac8I GCNNGC 4 cut(s) 88, 238, 242, 507
CaiI CAGNNNCTG 2 cut(s) 365, 758
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 475
Cfr42I CCGCGG 1 cut(s) 880
Csp6I GTAC 4 cut(s) 371, 618, 700, 906
CviAII CATG 8 cut(s) 182, 206, 479, 583, 717, 794, 822, 832
CviQI GTAC 4 cut(s) 371, 618, 700, 906
DdeI CTNAG 2 cut(s) 254, 814
DpnI GATC 7 cut(s) 186, 288, 357, 402, 518, 678, 762
DpnII GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
EaeI YGGCCR 2 cut(s) 568, 874
EciI GGCGGA 1 cut(s) 867
Eco130I CCWWGG 4 cut(s) 114, 478, 599, 831
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 475
Eco81I CCTNAGG 1 cut(s) 814
Eco91I GGTNACC 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 60, 358
EcoT14I CCWWGG 4 cut(s) 114, 478, 599, 831
ErhI CCWWGG 4 cut(s) 114, 478, 599, 831
FaeI CATG 8 cut(s) 185, 209, 482, 586, 720, 797, 825, 835
FaqI GGGAC 1 cut(s) 488
FatI CATG 8 cut(s) 181, 205, 478, 582, 716, 793, 821, 831
FauNDI CATATG 1 cut(s) 723
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 1 cut(s) 748
FokI GGATG 3 cut(s) 281, 301, 557
FspBI CTAG 2 cut(s) 54, 324
GlaI GCGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 647
HaeIII GGCC 3 cut(s) 381, 570, 876
HapII CCGG 4 cut(s) 506, 539, 953, 1053
HhaI GCGC 1 cut(s) 214
Hin1II CATG 8 cut(s) 185, 209, 482, 586, 720, 797, 825, 835
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HinfI GANTC 2 cut(s) 65, 1049
HpaII CCGG 4 cut(s) 506, 539, 953, 1053
HphI GGTGA 5 cut(s) 26, 719, 893, 899, 905
Hpy166II GTNNAC 4 cut(s) 618, 732, 749, 1023
Hpy188I TCNGA 5 cut(s) 319, 346, 516, 663, 760
Hpy188III TCNNGA 3 cut(s) 425, 520, 916
Hpy8I GTNNAC 4 cut(s) 618, 732, 749, 1023
Hpy99I CGWCG 1 cut(s) 922
HpyAV CCTTC 3 cut(s) 83, 462, 568
HpyCH4III ACNGT 5 cut(s) 22, 103, 415, 502, 1040
HpyCH4IV ACGT 3 cut(s) 201, 369, 942
HpyCH4V TGCA 8 cut(s) 45, 143, 160, 176, 244, 591, 737, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 858
HpyF3I CTNAG 2 cut(s) 254, 814
HpySE526I ACGT 3 cut(s) 201, 369, 942
Hsp92II CATG 8 cut(s) 185, 209, 482, 586, 720, 797, 825, 835
HspAI GCGC 1 cut(s) 212
KroI GCCGGC 1 cut(s) 505
KroNI GCCGGC 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 760
KspI CCGCGG 1 cut(s) 880
Kzo9I GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
LmnI GCTCC 1 cut(s) 362
LweI GCATC 3 cut(s) 223, 764, 961
MaeI CTAG 2 cut(s) 54, 324
MaeII ACGT 3 cut(s) 201, 369, 942
MaeIII GTNAC 2 cut(s) 409, 707
MalI GATC 7 cut(s) 186, 288, 357, 402, 518, 678, 762
MboI GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
MboII GAAGA 1 cut(s) 477
MfeI CAATTG 1 cut(s) 637
MflI RGATCY 1 cut(s) 355
MluCI AATT 9 cut(s) 30, 248, 300, 486, 494, 637, 765, 982, 1066
MlyI GAGTC 1 cut(s) 1058
MmeI TCCRAC 1 cut(s) 939
MroNI GCCGGC 1 cut(s) 505
MseI TTAA 4 cut(s) 29, 683, 768, 981
MspA1I CMGCKG 1 cut(s) 879
MspI CCGG 4 cut(s) 506, 539, 953, 1053
MspR9I CCNGG 3 cut(s) 62, 360, 1054
MunI CAATTG 1 cut(s) 637
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 2 cut(s) 62, 360
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 858
NaeI GCCGGC 1 cut(s) 507
NciI CCSGG 1 cut(s) 1054
NcoI CCATGG 2 cut(s) 478, 831
NdeI CATATG 1 cut(s) 723
NdeII GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
NgoMIV GCCGGC 1 cut(s) 505
NlaIII CATG 8 cut(s) 185, 209, 482, 586, 720, 797, 825, 835
NlaIV GGNNCC 4 cut(s) 357, 476, 510, 596
NmuCI GTSAC 1 cut(s) 707
NspI RCATGY 1 cut(s) 720
NspV TTCGAA 1 cut(s) 107
PciI ACATGT 1 cut(s) 716
PctI GAATGC 1 cut(s) 439
PdiI GCCGGC 1 cut(s) 507
PfeI GAWTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 358
PleI GAGTC 1 cut(s) 1057
PpsI GAGTC 1 cut(s) 1057
PscI ACATGT 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 60, 358
PspEI GGTNACC 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 2 cut(s) 60, 358
PspN4I GGNNCC 4 cut(s) 357, 476, 510, 596
PspPI GGNCC 1 cut(s) 475
PstI CTGCAG 2 cut(s) 162, 593
PstNI CAGNNNCTG 2 cut(s) 365, 758
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 4 cut(s) 372, 619, 701, 907
RsaNI GTAC 4 cut(s) 371, 618, 700, 906
SacII CCGCGG 1 cut(s) 880
SaqAI TTAA 4 cut(s) 29, 683, 768, 981
Sau3AI GATC 7 cut(s) 184, 286, 355, 400, 516, 676, 760
Sau96I GGNCC 1 cut(s) 475
SchI GAGTC 1 cut(s) 1058
ScrFI CCNGG 3 cut(s) 62, 360, 1054
SfaNI GCATC 3 cut(s) 223, 764, 961
SfcI CTRYAG 3 cut(s) 158, 589, 610
Sfr303I CCGCGG 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 107
SgrBI CCGCGG 1 cut(s) 880
SinI GGWCC 1 cut(s) 475
SmlI CTYRAG 1 cut(s) 742
SmoI CTYRAG 1 cut(s) 742
SpeI ACTAGT 1 cut(s) 323
Sse9I AATT 9 cut(s) 30, 248, 300, 486, 494, 637, 765, 982, 1066
SsiI CCGC 4 cut(s) 797, 852, 877, 879
SspI AATATT 1 cut(s) 265
SspMI CTAG 2 cut(s) 54, 324
StyD4I CCNGG 3 cut(s) 60, 358, 1052
StyI CCWWGG 4 cut(s) 114, 478, 599, 831
TaaI ACNGT 5 cut(s) 22, 103, 415, 502, 1040
TaiI ACGT 3 cut(s) 204, 372, 945
TaqI TCGA 1 cut(s) 107
TasI AATT 9 cut(s) 30, 248, 300, 486, 494, 637, 765, 982, 1066
TatI WGTACW 1 cut(s) 699
TauI GCSGC 2 cut(s) 800, 879
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 29, 683, 768, 981
Tru9I TTAA 4 cut(s) 29, 683, 768, 981
TscAI CASTG 2 cut(s) 739, 871
TseFI GTSAC 1 cut(s) 707
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 3 cut(s) 186, 194, 1017
TspRI CASTG 2 cut(s) 739, 871
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 2 cut(s) 300, 486
XceI RCATGY 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 748
XspI CTAG 2 cut(s) 54, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.