Rh1AG380300
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0010870
Physical Location & Seq
Forward (+)
61180320 .. 61182496
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG380300.1

Sequence Viewer

Length: 1047 bp
ATGGATAACATTAGTGAAACCGTTTCTTTAATTCACCGTGGTTGCAACTTGGCTAGAGACCTGGAATCAAACCTTCCCAACTTGCCAAGCCGACCCGAAACCGTTTCGAAATCCTTGGACGAGATAATCAGGGTTTTCGGTGCAGCAAGGGAGAGGCTGCAGAGTAGTAGTGCTGCACAACATGATCCAATGAGTTCATACGTTCATGTAGCGCAGCAGCAGCAGCAGATCGATGCGAGCTTGCAGGAATGGCTGAGGTGTAATTACACACAGGCAATGGAGGATCTTCACATCCAAACGCAGCGTTTTGCATCCGAAAAGAGTATTGCTGCTACTCAATCCGATATGAGGGATTCAGGAGCTGACGTACTTCAAGGCCAAATCCATCAGGCAATCGTTTCTCATGAACTGAATGCTGCTTCGTCCTCACAAAGCAGACCACGAAGAAGGAGGGACGATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCGGATCGGGAATACTGAAATCCCACCGGAGGATGGCTTCACGTGGCGAAAATACGGCCAGAAGGAGATCATGGGCTGCAGGTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATGTACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAAGTCTACCACCATCTGATCAAATTAAGCAAGAGACTATGACCCAAACTCATGCGGTAACCACTCAACCCTTAGGAACATGGCTCTCCATGGACTTCAACGCGAGAGGTGGAGGAAGCAGCAGTGGTGGCCGCGGTGATGGTGACGGTGACGGTGTAGGTACATCAATCACGACGACCACTCGTTACGGTAAAGACGTTGAGTTTCCGGTGGTGGATTTGGCTGATGCAATGTTTAATTCAGGGAGCAGCAGCAGTAATAGTATGGATTTTATGTTCACTTCATCTATGGACAGTAAATGGGAGTCCGGGGACAAGAAAAATTGA

Protein Analysis

348

Amino Acids

38.64

Weight (kDa)

6.07

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 174 - 234 2.9e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 727
AccII CGCG 2 cut(s) 824, 855
AciI CCGC 3 cut(s) 776, 853, 855
AclWI GGATC 4 cut(s) 179, 291, 503, 650
AcoI YGGCCR 2 cut(s) 547, 850
AcsI RAATTY 1 cut(s) 465
AcvI CACGTG 1 cut(s) 534
AfaI GTAC 4 cut(s) 369, 598, 680, 883
AfiI CCNNNNNNNGG 4 cut(s) 348, 497, 520, 524
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 3 cut(s) 374, 671, 820
AjnI CCWGG 1 cut(s) 60
AloI GAACNNNNNNTCC 2 cut(s) 980, 1012
AluBI AGCT 2 cut(s) 240, 362
AluI AGCT 2 cut(s) 240, 362
Alw26I GTCTC 2 cut(s) 51, 749
AlwI GGATC 4 cut(s) 179, 291, 503, 650
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 3 cut(s) 376, 547, 850
ApoI RAATTY 1 cut(s) 465
AspLEI GCGC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 5 cut(s) 26, 698, 869, 875, 881
AsuII TTCGAA 1 cut(s) 107
AxyI CCTNAGG 1 cut(s) 793
BanI GGYRCC 1 cut(s) 487
BbrPI CACGTG 1 cut(s) 534
BbvCI CCTCAGC 1 cut(s) 254
BccI CCATC 5 cut(s) 393, 452, 518, 742, 854
BceAI ACGGC 1 cut(s) 562
BciT130I CCWGG 1 cut(s) 62
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 2 cut(s) 51, 749
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 3 cut(s) 158, 568, 589
BfuAI ACCTGC 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 62, 1030
BmiI GGNNCC 2 cut(s) 489, 575
BmrFI CCNGG 2 cut(s) 62, 1030
BmsI GCATC 3 cut(s) 223, 320, 937
Bpu10I CCTNAGC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 706
BpuMI CCSGG 1 cut(s) 1030
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 7 cut(s) 37, 114, 491, 578, 810, 853, 1029
BsaWI WCCGGW 2 cut(s) 517, 928
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 4 cut(s) 348, 497, 520, 524
Bse118I RCCGGY 1 cut(s) 484
Bse21I CCTNAGG 1 cut(s) 793
Bse3DI GCAATG 2 cut(s) 282, 957
BseBI CCWGG 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 7 cut(s) 37, 114, 491, 578, 810, 853, 1029
BseGI GGATG 3 cut(s) 291, 311, 529
BseLI CCNNNNNNNGG 4 cut(s) 348, 497, 520, 524
BseMI GCAATG 2 cut(s) 282, 957
BseMII CTCAG 1 cut(s) 245
BseYI CCCAGC 1 cut(s) 622
BsgI GTGCAG 3 cut(s) 159, 162, 699
Bsh1236I CGCG 2 cut(s) 824, 855
BshFI GGCC 3 cut(s) 378, 549, 852
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 231
BsiSI CCGG 4 cut(s) 485, 518, 929, 1029
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 348, 497, 520, 524
BsmAI GTCTC 2 cut(s) 51, 749
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 3 cut(s) 378, 549, 852
Bso31I GGTCTC 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 678
Bsp143I GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
Bsp19I CCATGG 1 cut(s) 810
BspACI CCGC 3 cut(s) 776, 853, 855
BspANI GGCC 3 cut(s) 378, 549, 852
BspCNI CTCAG 1 cut(s) 246
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 2 cut(s) 824, 855
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 489, 575
BspMAI CTGCAG 2 cut(s) 162, 572
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 4 cut(s) 179, 291, 503, 650
BspT104I TTCGAA 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 282, 957
BsrFI RCCGGY 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 678
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 7 cut(s) 37, 114, 491, 578, 810, 853, 1029
BssMI GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
BssT1I CCWWGG 3 cut(s) 114, 578, 810
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 8 cut(s) 22, 38, 103, 481, 869, 875, 911, 1016
BstAUI TGTACA 1 cut(s) 678
BstBAI YACGTR 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 238, 242, 486
BstDEI CTNAG 2 cut(s) 254, 793
BstDSI CCRYGG 3 cut(s) 37, 810, 853
BstEII GGTNACC 2 cut(s) 686, 778
BstF5I GGATG 3 cut(s) 291, 311, 529
BstFNI CGCG 2 cut(s) 824, 855
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 7 cut(s) 187, 231, 286, 498, 561, 658, 742
BstMAI GTCTC 2 cut(s) 51, 749
BstMBI GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
BstMWI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 849
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 699
BstPI GGTNACC 2 cut(s) 686, 778
BstSCI CCNGG 2 cut(s) 60, 1028
BstSFI CTRYAG 3 cut(s) 158, 568, 589
BstUI CGCG 2 cut(s) 824, 855
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 793
BsuRI GGCC 3 cut(s) 378, 549, 852
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 3 cut(s) 37, 810, 853
BtsCI GGATG 3 cut(s) 291, 311, 529
BtsI GCAGTG 2 cut(s) 711, 850
BtsIMutI CAGTG 2 cut(s) 711, 850
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 3 cut(s) 238, 242, 486
CaiI CAGNNNCTG 1 cut(s) 362
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 484
Cfr42I CCGCGG 1 cut(s) 856
ClaI ATCGAT 1 cut(s) 231
Csp6I GTAC 4 cut(s) 368, 597, 679, 882
CviAII CATG 8 cut(s) 182, 206, 404, 562, 696, 773, 801, 811
CviQI GTAC 4 cut(s) 368, 597, 679, 882
DdeI CTNAG 2 cut(s) 254, 793
DpnI GATC 7 cut(s) 186, 230, 285, 497, 560, 657, 741
DpnII GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
EaeI YGGCCR 2 cut(s) 547, 850
Eco130I CCWWGG 3 cut(s) 114, 578, 810
Eco31I GGTCTC 1 cut(s) 51
Eco72I CACGTG 1 cut(s) 534
Eco81I CCTNAGG 1 cut(s) 793
Eco91I GGTNACC 2 cut(s) 686, 778
EcoO65I GGTNACC 2 cut(s) 686, 778
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 3 cut(s) 114, 578, 810
ErhI CCWWGG 3 cut(s) 114, 578, 810
FaeI CATG 8 cut(s) 185, 209, 407, 565, 699, 776, 804, 814
FaqI GGGAC 1 cut(s) 467
FatI CATG 8 cut(s) 181, 205, 403, 561, 695, 772, 800, 810
FauNDI CATATG 1 cut(s) 702
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 727
FokI GGATG 3 cut(s) 278, 298, 536
FspBI CTAG 1 cut(s) 54
GlaI GCGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 626
HaeIII GGCC 3 cut(s) 378, 549, 852
HapII CCGG 4 cut(s) 485, 518, 929, 1029
HhaI GCGC 1 cut(s) 214
Hin1II CATG 8 cut(s) 185, 209, 407, 565, 699, 776, 804, 814
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HinfI GANTC 3 cut(s) 65, 353, 1025
HpaII CCGG 4 cut(s) 485, 518, 929, 1029
HphI GGTGA 5 cut(s) 26, 698, 869, 875, 881
Hpy166II GTNNAC 4 cut(s) 597, 711, 728, 999
Hpy188I TCNGA 5 cut(s) 316, 343, 495, 642, 739
Hpy188III TCNNGA 4 cut(s) 357, 404, 499, 892
Hpy8I GTNNAC 4 cut(s) 597, 711, 728, 999
Hpy99I CGWCG 1 cut(s) 898
HpyAV CCTTC 3 cut(s) 83, 441, 547
HpyCH4III ACNGT 8 cut(s) 22, 38, 103, 481, 869, 875, 911, 1016
HpyCH4IV ACGT 4 cut(s) 201, 366, 533, 918
HpyCH4V TGCA 9 cut(s) 45, 143, 160, 176, 244, 311, 570, 716, 950
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 849
HpyF3I CTNAG 2 cut(s) 254, 793
HpySE526I ACGT 4 cut(s) 201, 366, 533, 918
Hsp92II CATG 8 cut(s) 185, 209, 407, 565, 699, 776, 804, 814
HspAI GCGC 1 cut(s) 212
KroI GCCGGC 1 cut(s) 484
KroNI GCCGGC 1 cut(s) 486
Ksp22I TGATCA 1 cut(s) 739
KspI CCGCGG 1 cut(s) 856
Kzo9I GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
LmnI GCTCC 2 cut(s) 359, 966
LweI GCATC 3 cut(s) 223, 320, 937
MaeI CTAG 1 cut(s) 54
MaeII ACGT 4 cut(s) 201, 366, 533, 918
MaeIII GTNAC 5 cut(s) 686, 778, 863, 869, 905
MalI GATC 7 cut(s) 186, 230, 285, 497, 560, 657, 741
MboI GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
MboII GAAGA 2 cut(s) 278, 456
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 1 cut(s) 283
MluCI AATT 8 cut(s) 30, 262, 465, 473, 616, 744, 958, 1042
MlyI GAGTC 1 cut(s) 1034
MroNI GCCGGC 1 cut(s) 484
MseI TTAA 4 cut(s) 29, 662, 747, 957
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 4 cut(s) 485, 518, 929, 1029
MspR9I CCNGG 2 cut(s) 62, 1030
MunI CAATTG 1 cut(s) 616
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 62
MvnI CGCG 2 cut(s) 824, 855
MwoI GCNNNNNNNGC 4 cut(s) 220, 223, 250, 849
NaeI GCCGGC 1 cut(s) 486
NciI CCSGG 1 cut(s) 1030
NcoI CCATGG 1 cut(s) 810
NdeI CATATG 1 cut(s) 702
NdeII GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
NgoMIV GCCGGC 1 cut(s) 484
NlaIII CATG 8 cut(s) 185, 209, 407, 565, 699, 776, 804, 814
NlaIV GGNNCC 2 cut(s) 489, 575
NmuCI GTSAC 3 cut(s) 686, 863, 869
NspI RCATGY 1 cut(s) 699
NspV TTCGAA 1 cut(s) 107
PagI TCATGA 1 cut(s) 403
PciI ACATGT 1 cut(s) 695
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PctI GAATGC 1 cut(s) 418
PdiI GCCGGC 1 cut(s) 486
PfeI GAWTC 2 cut(s) 65, 353
PleI GAGTC 1 cut(s) 1033
PmaCI CACGTG 1 cut(s) 534
PmlI CACGTG 1 cut(s) 534
PpsI GAGTC 1 cut(s) 1033
Ppu21I YACGTR 1 cut(s) 534
PscI ACATGT 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 60
PspCI CACGTG 1 cut(s) 534
PspEI GGTNACC 2 cut(s) 686, 778
PspFI CCCAGC 1 cut(s) 622
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 489, 575
PstI CTGCAG 2 cut(s) 162, 572
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 4 cut(s) 369, 598, 680, 883
RsaNI GTAC 4 cut(s) 368, 597, 679, 882
SacII CCGCGG 1 cut(s) 856
SaqAI TTAA 4 cut(s) 29, 662, 747, 957
Sau3AI GATC 7 cut(s) 184, 228, 283, 495, 558, 655, 739
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 2 cut(s) 62, 1030
SfaNI GCATC 3 cut(s) 223, 320, 937
SfcI CTRYAG 3 cut(s) 158, 568, 589
Sfr303I CCGCGG 1 cut(s) 856
SfuI TTCGAA 1 cut(s) 107
SgrBI CCGCGG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 721
SmoI CTYRAG 1 cut(s) 721
Sse9I AATT 8 cut(s) 30, 262, 465, 473, 616, 744, 958, 1042
SsiI CCGC 3 cut(s) 776, 853, 855
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 60, 1028
StyI CCWWGG 3 cut(s) 114, 578, 810
TaaI ACNGT 8 cut(s) 22, 38, 103, 481, 869, 875, 911, 1016
TaiI ACGT 4 cut(s) 204, 369, 536, 921
TaqI TCGA 2 cut(s) 107, 231
TasI AATT 8 cut(s) 30, 262, 465, 473, 616, 744, 958, 1042
TatI WGTACW 1 cut(s) 678
TauI GCSGC 1 cut(s) 855
TfiI GAWTC 2 cut(s) 65, 353
Tru1I TTAA 4 cut(s) 29, 662, 747, 957
Tru9I TTAA 4 cut(s) 29, 662, 747, 957
TscAI CASTG 2 cut(s) 718, 850
TseFI GTSAC 3 cut(s) 686, 863, 869
Tsp45I GTSAC 3 cut(s) 686, 863, 869
TspDTI ATGAA 4 cut(s) 186, 194, 420, 993
TspRI CASTG 2 cut(s) 718, 850
XapI RAATTY 1 cut(s) 465
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 727
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.