Rh1BG343900
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010870
Physical Location & Seq
Forward (+)
47718687 .. 47720953
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG343900.1

Sequence Viewer

Length: 1176 bp
ATGGAGCACCAGCACAGAAGCCTCACCACAGCTTACATTCGCTACCCTTTTAAATTTCACCACAACATTTTCAAAAATACGACAAGCCAAACCAAGCAAACCATGGATAACATTAGTGAAACCGTTTCTTTAATTCACCATGGTTGCAACTTGGCTAGAGACCTGGAATCAAACCTTCCCAACTTGGCAAGCCGACCCGAAACCGTTTCGAAATCCTTGGACGAGATATTGAGGGTTTTCGGTGCAGCAAGAGAGAGGCTGGAGAGTAGTAGTGCTGCACAACATGATCCAATGAGTTCATACGTTCATGTAGCGCAGCAGCAGCAGCAGATCGATGCGAGCTTGCAGGAATGGCTGAGGTGTAATTACACACAGGCAATGGAGGATCTTCACATCCAAACGCAGCGTTTTGCATCCGAAAAGAGTAGTGCTGCTACTCAATCCGATATGAGGGATTCAGGAGCTGACGTACTTCAAGGCCAAATCCATCAGGCAATGGATCGTATTGTAACAGTCGTTTCTCATGAACTGAATGCTGCTTCGTCCTCACAAAGCAGACCACGAAGAAGGAGGGACGATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCGGATCGGGAATACTGAAATCCCACCGGAGGATGGCTTCACTTGGCGAAAATACGGCCAGAAGGAGATCATGGGCTGCAGGTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATGTACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAAGTCTACCACCATCTGATCAAATTAAGCAAGAGACTATGACCCAAACTCATGCGGCAACCACTCAACCCTTAGGAACATGGCTCTCCATGGACTTCAACGCGAGAGGTGGAGGAAGCAGCAGTGGTGGTGGCCGCGGTGATGGTGATGGTGATGGTGACGGTGTAGGTACATCAATCACGACAACCACTCGTTATGGTAAAGACGTTGAGTTTCCGGTGGTGGATTTGGCTGATGCAATGTTTAATTCAGGGAGTAGCAGCAGTAATAGTATGGATTTTATGTTCACTTCATCTATGGACAGTAAATGGGAGTCCGGGGACAAGAAAAATTGA

Protein Analysis

391

Amino Acids

43.64

Weight (kDa)

6.43

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 214 - 274 3.5e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 681
AccB1I GGYRCC 1 cut(s) 607
AccI GTMKAC 1 cut(s) 847
AccII CGCG 2 cut(s) 944, 978
AciI CCGC 3 cut(s) 896, 976, 978
AclWI GGATC 5 cut(s) 281, 393, 507, 623, 770
AcoI YGGCCR 2 cut(s) 667, 973
AcsI RAATTY 2 cut(s) 53, 585
AfaI GTAC 4 cut(s) 471, 718, 800, 1012
AfiI CCNNNNNNNGG 4 cut(s) 450, 617, 640, 644
AflIII ACRYGT 1 cut(s) 815
AgsI TTSAA 4 cut(s) 73, 476, 791, 940
AjnI CCWGG 1 cut(s) 162
AloI GAACNNNNNNTCC 2 cut(s) 1109, 1141
AluBI AGCT 3 cut(s) 32, 342, 464
AluI AGCT 3 cut(s) 32, 342, 464
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 153, 869
AlwI GGATC 5 cut(s) 281, 393, 507, 623, 770
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 3 cut(s) 478, 667, 973
ApoI RAATTY 2 cut(s) 53, 585
AspLEI GCGC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 1159
AsuHPI GGTGA 8 cut(s) 16, 50, 128, 818, 992, 998, 1004, 1010
AsuII TTCGAA 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 913
BanI GGYRCC 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 9
BbvCI CCTCAGC 1 cut(s) 356
BccI CCATC 7 cut(s) 495, 572, 638, 862, 977, 983, 989
BceAI ACGGC 1 cut(s) 682
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 1 cut(s) 1159
BcoDI GTCTC 2 cut(s) 153, 869
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 2 cut(s) 688, 709
BfuAI ACCTGC 1 cut(s) 681
Bme1390I CCNGG 2 cut(s) 164, 1159
BmiI GGNNCC 2 cut(s) 609, 695
BmrFI CCNGG 2 cut(s) 164, 1159
BmsI GCATC 3 cut(s) 325, 422, 1066
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 826
BpuMI CCSGG 1 cut(s) 1159
Bsa29I ATCGAT 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 8 cut(s) 102, 139, 216, 611, 698, 930, 976, 1158
BsaWI WCCGGW 2 cut(s) 637, 1057
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 450, 617, 640, 644
Bse118I RCCGGY 1 cut(s) 604
Bse21I CCTNAGG 1 cut(s) 913
Bse3DI GCAATG 3 cut(s) 384, 501, 1086
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 8 cut(s) 102, 139, 216, 611, 698, 930, 976, 1158
BseGI GGATG 3 cut(s) 393, 413, 649
BseLI CCNNNNNNNGG 4 cut(s) 450, 617, 640, 644
BseMI GCAATG 3 cut(s) 384, 501, 1086
BseMII CTCAG 1 cut(s) 347
BseYI CCCAGC 1 cut(s) 742
BsgI GTGCAG 3 cut(s) 261, 264, 819
Bsh1236I CGCG 2 cut(s) 944, 978
BshFI GGCC 3 cut(s) 480, 669, 975
BshNI GGYRCC 1 cut(s) 607
BshVI ATCGAT 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 4 cut(s) 605, 638, 1058, 1158
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 4 cut(s) 450, 617, 640, 644
BsmAI GTCTC 2 cut(s) 153, 869
BsmFI GGGAC 1 cut(s) 587
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 3 cut(s) 480, 669, 975
Bso31I GGTCTC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
Bsp19I CCATGG 3 cut(s) 102, 139, 930
BspACI CCGC 3 cut(s) 896, 976, 978
BspANI GGCC 3 cut(s) 480, 669, 975
BspCNI CTCAG 1 cut(s) 348
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 2 cut(s) 944, 978
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 609, 695
BspMAI CTGCAG 1 cut(s) 692
BspMI ACCTGC 1 cut(s) 681
BspPI GGATC 5 cut(s) 281, 393, 507, 623, 770
BspT104I TTCGAA 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 607
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 3 cut(s) 384, 501, 1086
BsrFI RCCGGY 1 cut(s) 604
BsrGI TGTACA 1 cut(s) 798
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 8 cut(s) 102, 139, 216, 611, 698, 930, 976, 1158
BssMI GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
BssT1I CCWWGG 5 cut(s) 102, 139, 216, 698, 930
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 6 cut(s) 124, 205, 514, 601, 1004, 1145
BstAUI TGTACA 1 cut(s) 798
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 4 cut(s) 190, 340, 344, 606
BstDEI CTNAG 2 cut(s) 356, 913
BstDSI CCRYGG 4 cut(s) 102, 139, 930, 976
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 3 cut(s) 393, 413, 649
BstFNI CGCG 2 cut(s) 944, 978
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 8 cut(s) 289, 333, 388, 502, 618, 681, 778, 862
BstMAI GTCTC 2 cut(s) 153, 869
BstMBI GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
BstMWI GCNNNNNNNGC 3 cut(s) 322, 325, 352
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 819
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 2 cut(s) 162, 1157
BstSFI CTRYAG 2 cut(s) 688, 709
BstUI CGCG 2 cut(s) 944, 978
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 333
Bsu36I CCTNAGG 1 cut(s) 913
BsuRI GGCC 3 cut(s) 480, 669, 975
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 4 cut(s) 102, 139, 930, 976
BtsCI GGATG 3 cut(s) 393, 413, 649
BtsI GCAGTG 2 cut(s) 831, 970
BtsIMutI CAGTG 2 cut(s) 831, 970
BveI ACCTGC 1 cut(s) 681
Cac8I GCNNGC 4 cut(s) 190, 340, 344, 606
CaiI CAGNNNCTG 1 cut(s) 464
CciI TCATGA 1 cut(s) 523
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 604
Cfr42I CCGCGG 1 cut(s) 979
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 4 cut(s) 470, 717, 799, 1011
CviQI GTAC 4 cut(s) 470, 717, 799, 1011
DdeI CTNAG 2 cut(s) 356, 913
DpnI GATC 8 cut(s) 288, 332, 387, 501, 617, 680, 777, 861
DpnII GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 2 cut(s) 667, 973
Eco130I CCWWGG 5 cut(s) 102, 139, 216, 698, 930
Eco31I GGTCTC 1 cut(s) 153
Eco81I CCTNAGG 1 cut(s) 913
Eco91I GGTNACC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 5 cut(s) 102, 139, 216, 698, 930
ErhI CCWWGG 5 cut(s) 102, 139, 216, 698, 930
FaqI GGGAC 1 cut(s) 587
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 1 cut(s) 859
FblI GTMKAC 1 cut(s) 847
FokI GGATG 3 cut(s) 380, 400, 656
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 315
GsaI CCCAGC 1 cut(s) 746
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 3 cut(s) 480, 669, 975
HapII CCGG 4 cut(s) 605, 638, 1058, 1158
HhaI GCGC 1 cut(s) 316
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HinfI GANTC 3 cut(s) 167, 455, 1154
HpaII CCGG 4 cut(s) 605, 638, 1058, 1158
HphI GGTGA 8 cut(s) 16, 50, 128, 818, 992, 998, 1004, 1010
Hpy166II GTNNAC 4 cut(s) 717, 831, 848, 1128
Hpy188I TCNGA 5 cut(s) 418, 445, 615, 762, 859
Hpy188III TCNNGA 4 cut(s) 459, 524, 619, 1021
Hpy8I GTNNAC 4 cut(s) 717, 831, 848, 1128
HpyAV CCTTC 3 cut(s) 185, 561, 667
HpyCH4III ACNGT 6 cut(s) 124, 205, 514, 601, 1004, 1145
HpyCH4IV ACGT 3 cut(s) 303, 468, 1047
HpyCH4V TGCA 8 cut(s) 147, 245, 278, 346, 413, 690, 836, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 325, 352
HpyF3I CTNAG 2 cut(s) 356, 913
HpySE526I ACGT 3 cut(s) 303, 468, 1047
HspAI GCGC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
Ksp22I TGATCA 1 cut(s) 859
KspI CCGCGG 1 cut(s) 979
Kzo9I GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
LmnI GCTCC 2 cut(s) 4, 461
LweI GCATC 3 cut(s) 325, 422, 1066
MaeI CTAG 1 cut(s) 156
MaeII ACGT 3 cut(s) 303, 468, 1047
MaeIII GTNAC 3 cut(s) 508, 806, 998
MalI GATC 8 cut(s) 288, 332, 387, 501, 617, 680, 777, 861
MboI GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
MboII GAAGA 2 cut(s) 380, 576
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 9 cut(s) 53, 132, 364, 585, 593, 736, 864, 1087, 1171
MlyI GAGTC 1 cut(s) 1163
MroNI GCCGGC 1 cut(s) 604
MseI TTAA 5 cut(s) 51, 131, 782, 867, 1086
MspA1I CMGCKG 1 cut(s) 978
MspI CCGG 4 cut(s) 605, 638, 1058, 1158
MspR9I CCNGG 2 cut(s) 164, 1159
MunI CAATTG 1 cut(s) 736
Mva1269I GAATGC 1 cut(s) 538
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 944, 978
MwoI GCNNNNNNNGC 3 cut(s) 322, 325, 352
NaeI GCCGGC 1 cut(s) 606
NciI CCSGG 1 cut(s) 1159
NcoI CCATGG 3 cut(s) 102, 139, 930
NdeI CATATG 1 cut(s) 822
NdeII GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
NgoMIV GCCGGC 1 cut(s) 604
NlaIV GGNNCC 2 cut(s) 609, 695
NmuCI GTSAC 2 cut(s) 806, 998
NspI RCATGY 1 cut(s) 819
NspV TTCGAA 1 cut(s) 209
PagI TCATGA 1 cut(s) 523
PciI ACATGT 1 cut(s) 815
PctI GAATGC 1 cut(s) 538
PdiI GCCGGC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 167, 455
PleI GAGTC 1 cut(s) 1162
PpsI GAGTC 1 cut(s) 1162
PscI ACATGT 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 162
PspEI GGTNACC 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 609, 695
PstI CTGCAG 1 cut(s) 692
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 4 cut(s) 471, 718, 800, 1012
RsaNI GTAC 4 cut(s) 470, 717, 799, 1011
SacII CCGCGG 1 cut(s) 979
SaqAI TTAA 5 cut(s) 51, 131, 782, 867, 1086
Sau3AI GATC 8 cut(s) 286, 330, 385, 499, 615, 678, 775, 859
SchI GAGTC 1 cut(s) 1163
ScrFI CCNGG 2 cut(s) 164, 1159
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 3 cut(s) 325, 422, 1066
SfcI CTRYAG 2 cut(s) 688, 709
Sfr303I CCGCGG 1 cut(s) 979
SfuI TTCGAA 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 979
SmlI CTYRAG 1 cut(s) 841
SmoI CTYRAG 1 cut(s) 841
Sse9I AATT 9 cut(s) 53, 132, 364, 585, 593, 736, 864, 1087, 1171
SsiI CCGC 3 cut(s) 896, 976, 978
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 162, 1157
StyI CCWWGG 5 cut(s) 102, 139, 216, 698, 930
TaaI ACNGT 6 cut(s) 124, 205, 514, 601, 1004, 1145
TaiI ACGT 3 cut(s) 306, 471, 1050
TaqI TCGA 2 cut(s) 209, 333
TasI AATT 9 cut(s) 53, 132, 364, 585, 593, 736, 864, 1087, 1171
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 899, 978
TfiI GAWTC 2 cut(s) 167, 455
Tru1I TTAA 5 cut(s) 51, 131, 782, 867, 1086
Tru9I TTAA 5 cut(s) 51, 131, 782, 867, 1086
TscAI CASTG 2 cut(s) 838, 970
TseFI GTSAC 2 cut(s) 806, 998
Tsp45I GTSAC 2 cut(s) 806, 998
TspDTI ATGAA 4 cut(s) 288, 296, 540, 1122
TspRI CASTG 2 cut(s) 838, 970
XapI RAATTY 2 cut(s) 53, 585
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 847
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.