MD01G1168500.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010870
Physical Location & Seq
Forward (+)
27285229 .. 27286785
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1168500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGACCCCAATAAAGCATATAAAGTTCTTTCCTATTTGTTTTCTAATCATATTATTTTATGTTCATTTTGTTTACTTAAATAGGAAAGATCAAGGGGTAATTAACAAAATAACAGTGCCAGCACCAAGGATTGGAAACACTGAAATCCCACCAGAAGATGGCTTCACCTGGAGAAAATACGGCCAAAAGGAGATCATGAACAGCAAATTCCCAAGGGGTTACTATAGATGCACCCATCAAAAGCTGTACAATTGCCCAGCCAAGAAGCAAGTCCAGAGGCTGGACAATGATCCCTTAACATTTGAGGTAATGTACAGAGGTGAACATACATGCCACATGTCAGCCACTGCACCTTCAACTCTACCACCACCAACAGCAGACCATCAAATTCCACAAGATCATCTGTCAGCAACCACTGAGCCTCCAACATCTCTATGGCTTTCCATGGACTTTAACCCTATTAGACAAGGAGGCAGCAGCAGTGGTATGATCGGTGGCCGTGGCAGTGGTCGTGGCAGCGGTGATGACGTTGGAACATCCACCGGCACACGTTATGGTAAAGAAGTTGACTTTCCTGTGATGGATTTGGCCGATACTATGTTTAATTCAGGCAGCAGCAGCAGTAGCATGGATTTTATTTTCAATTCTGCAGAACATAGATGGGAGTCAGATGACAAGAAAAATTGA

Protein Analysis

229

Amino Acids

25.66

Weight (kDa)

7.72

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 52 - 112 7e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 131, 158, 280
AciI CCGC 1 cut(s) 519
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 3 cut(s) 181, 496, 588
AcsI RAATTY 2 cut(s) 206, 387
AfaI GTAC 2 cut(s) 248, 314
AfiI CCNNNNNNNGG 3 cut(s) 131, 158, 280
AflIII ACRYGT 2 cut(s) 336, 548
AgsI TTSAA 2 cut(s) 357, 643
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
AlwI GGATC 1 cut(s) 284
AlwNI CAGNNNCTG 2 cut(s) 280, 347
AoxI GGCC 3 cut(s) 181, 496, 588
ApeKI GCWGC 6 cut(s) 474, 477, 516, 612, 615, 618
ApoI RAATTY 2 cut(s) 206, 387
AsuHPI GGTGA 3 cut(s) 157, 332, 533
BbvI GCAGC 6 cut(s) 486, 489, 528, 624, 627, 630
BccI CCATC 5 cut(s) 152, 243, 390, 574, 654
BceAI ACGGC 2 cut(s) 196, 483
BciT130I CCWGG 1 cut(s) 169
BfmI CTRYAG 2 cut(s) 223, 648
BisI GCNGC 6 cut(s) 475, 478, 517, 613, 616, 619
BlsI GCNGC 6 cut(s) 476, 479, 518, 614, 617, 620
Bme1390I CCNGG 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 125, 212, 444, 499
BsaXI ACNNNNNCTCC 2 cut(s) 406, 436
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 158, 280
Bse118I RCCGGY 1 cut(s) 542
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 4 cut(s) 125, 212, 444, 499
BseGI GGATG 1 cut(s) 536
BseLI CCNNNNNNNGG 3 cut(s) 131, 158, 280
BseMII CTCAG 1 cut(s) 408
BseXI GCAGC 6 cut(s) 486, 489, 528, 624, 627, 630
BseYI CCCAGC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 3 cut(s) 183, 498, 590
BsiSI CCGG 1 cut(s) 543
BslI CCNNNNNNNGG 3 cut(s) 131, 158, 280
BsnI GGCC 3 cut(s) 183, 498, 590
Bsp1407I TGTACA 2 cut(s) 246, 312
Bsp143I GATC 5 cut(s) 88, 192, 289, 397, 489
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 1 cut(s) 519
BspANI GGCC 3 cut(s) 183, 498, 590
BspCNI CTCAG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 542
BsrGI TGTACA 2 cut(s) 246, 312
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 4 cut(s) 125, 212, 444, 499
BssMI GATC 5 cut(s) 88, 192, 289, 397, 489
BssT1I CCWWGG 3 cut(s) 125, 212, 444
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 115
BstAUI TGTACA 2 cut(s) 246, 312
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 2 cut(s) 444, 499
BstF5I GGATG 1 cut(s) 536
BstKTI GATC 5 cut(s) 91, 195, 292, 400, 492
BstMBI GATC 5 cut(s) 88, 192, 289, 397, 489
BstMWI GCNNNNNNNGC 2 cut(s) 618, 624
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 2 cut(s) 333, 340
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 2 cut(s) 223, 648
BstV1I GCAGC 6 cut(s) 486, 489, 528, 624, 627, 630
BsuRI GGCC 3 cut(s) 183, 498, 590
BtgI CCRYGG 2 cut(s) 444, 499
BtsCI GGATG 1 cut(s) 536
BtsI GCAGTG 3 cut(s) 345, 487, 511
BtsIMutI CAGTG 6 cut(s) 120, 138, 345, 414, 487, 511
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 2 cut(s) 280, 347
CciI TCATGA 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 542
Csp6I GTAC 2 cut(s) 247, 313
CviAII CATG 5 cut(s) 196, 330, 337, 445, 628
CviQI GTAC 2 cut(s) 247, 313
DdeI CTNAG 1 cut(s) 417
DpnI GATC 5 cut(s) 90, 194, 291, 399, 491
DpnII GATC 5 cut(s) 88, 192, 289, 397, 489
EaeI YGGCCR 3 cut(s) 181, 496, 588
Eco130I CCWWGG 3 cut(s) 125, 212, 444
EcoRII CCWGG 1 cut(s) 167
EcoT14I CCWWGG 3 cut(s) 125, 212, 444
ErhI CCWWGG 3 cut(s) 125, 212, 444
FaeI CATG 5 cut(s) 199, 333, 340, 448, 631
FatI CATG 5 cut(s) 195, 329, 336, 444, 627
Fnu4HI GCNGC 6 cut(s) 475, 478, 517, 613, 616, 619
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 6 cut(s) 475, 478, 517, 613, 616, 619
GluI GCNGC 6 cut(s) 475, 478, 517, 613, 616, 619
GsaI CCCAGC 1 cut(s) 260
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 3 cut(s) 183, 498, 590
HapII CCGG 1 cut(s) 543
Hin1II CATG 5 cut(s) 199, 333, 340, 448, 631
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 1 cut(s) 665
HpaII CCGG 1 cut(s) 543
HphI GGTGA 3 cut(s) 157, 332, 533
Hpy166II GTNNAC 3 cut(s) 73, 323, 568
Hpy188I TCNGA 1 cut(s) 670
Hpy188III TCNNGA 2 cut(s) 196, 274
Hpy8I GTNNAC 3 cut(s) 73, 323, 568
HpyAV CCTTC 1 cut(s) 363
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 528, 550
HpyCH4V TGCA 3 cut(s) 231, 350, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 618, 624
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 2 cut(s) 528, 550
Hsp92II CATG 5 cut(s) 199, 333, 340, 448, 631
Kzo9I GATC 5 cut(s) 88, 192, 289, 397, 489
Lsp1109I GCAGC 6 cut(s) 486, 489, 528, 624, 627, 630
LweI GCATC 1 cut(s) 218
MaeII ACGT 2 cut(s) 528, 550
MaeIII GTNAC 1 cut(s) 218
MalI GATC 5 cut(s) 90, 194, 291, 399, 491
MboI GATC 5 cut(s) 88, 192, 289, 397, 489
MboII GAAGA 1 cut(s) 167
MfeI CAATTG 1 cut(s) 250
MluCI AATT 7 cut(s) 99, 206, 250, 387, 604, 643, 682
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 2 cut(s) 449, 511
MnlI CCTC 5 cut(s) 270, 298, 311, 432, 464
MseI TTAA 5 cut(s) 77, 102, 296, 453, 603
MslI CAYNNNNRTG 1 cut(s) 433
MspA1I CMGCKG 1 cut(s) 519
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 169
MunI CAATTG 1 cut(s) 250
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 2 cut(s) 618, 624
NcoI CCATGG 1 cut(s) 444
NdeII GATC 5 cut(s) 88, 192, 289, 397, 489
NlaIII CATG 5 cut(s) 199, 333, 340, 448, 631
NspI RCATGY 2 cut(s) 333, 340
PagI TCATGA 1 cut(s) 195
PciI ACATGT 1 cut(s) 336
PflMI CCANNNNNTGG 3 cut(s) 131, 158, 280
PkrI GCNGC 6 cut(s) 476, 479, 518, 614, 617, 620
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
PscI ACATGT 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 167
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 2 cut(s) 280, 347
RsaI GTAC 2 cut(s) 248, 314
RsaNI GTAC 2 cut(s) 247, 313
RseI CAYNNNNRTG 1 cut(s) 433
SaqAI TTAA 5 cut(s) 77, 102, 296, 453, 603
SatI GCNGC 6 cut(s) 475, 478, 517, 613, 616, 619
Sau3AI GATC 5 cut(s) 88, 192, 289, 397, 489
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 169
SetI ASST 7 cut(s) 170, 246, 309, 322, 355, 531, 553
SfaNI GCATC 1 cut(s) 218
SfcI CTRYAG 2 cut(s) 223, 648
SmiMI CAYNNNNRTG 1 cut(s) 433
Sse9I AATT 7 cut(s) 99, 206, 250, 387, 604, 643, 682
SsiI CCGC 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 3 cut(s) 125, 212, 444
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 531, 553
TasI AATT 7 cut(s) 99, 206, 250, 387, 604, 643, 682
TatI WGTACW 2 cut(s) 246, 312
Tru1I TTAA 5 cut(s) 77, 102, 296, 453, 603
Tru9I TTAA 5 cut(s) 77, 102, 296, 453, 603
TscAI CASTG 6 cut(s) 120, 145, 352, 421, 487, 511
TseI GCWGC 6 cut(s) 474, 477, 516, 612, 615, 618
TspDTI ATGAA 2 cut(s) 53, 212
TspRI CASTG 6 cut(s) 120, 145, 352, 421, 487, 511
Van91I CCANNNNNTGG 3 cut(s) 131, 158, 280
XapI RAATTY 2 cut(s) 206, 387
XceI RCATGY 2 cut(s) 333, 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.