MD07G1234600.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010870
Physical Location & Seq
Forward (+)
30813251 .. 30815403
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1234600.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGATGACATCATTTCTTTAATCCTCCATGGATGCGAGTTGGCTAGAGAGCTTGGAGAAACCCTACCCAACTTGGCCACCCAACCTGACTCACTTTTCAATTCATTGGAAAAAATCACAAAGATTTTTGGTTCAGCAAAGGAAAGGGTTTTACATAGTCAAGATTCAAGTAGTAGTTCATATCTTCACAACACTTTAACACTTGAGCACCAGCAGATTGGAACAAGTGTTGAACAATGGCTGAGGTCTAATCCGCAGGCAATGGATATAATCCAAACCCAACTAGCAGCTGATCGAAACGAAGCGAAGATCAGTGGCGGCATTGATATCCGGGGTTTGGAGGCGGCAGTTAGGGGTTCTCAGGCAGTGGATGTTGTGCCAGCTTCTGACTTGAACATTGCTTCATCAAGCTCACAAAGACCGCGACAAAGGAAAGATCAAGGGTTAATTAGCAAAATGACAGTGCCAGCACCTAGGATTGGAAACACTGAAATCCCACCAGAGGATGGCTTCACTTGGAGAAAATATGGTCAAAAGGAGATCATAAACAGCAGATTCCCAAGGAGTTACTATAGATGCACCCATCAAAAGTTGTACAATTGCCTGGCCAAGAAGCATGTCCAGAGGCTGGACGATGATCCCTCGACATTTGAAGTAACGTACATAGGTGAACACACATGCCACATGTCAGCCACTGCACCTTCAATTCTAACACCCACAGCAGATCATCATATTACACAAGATCAGATGGCAGCAACCACTGAGCCTCCAACATCTCGATGGCTTTCCATGGACTTTAACCCTATTAGAGGGGGCAGCAGCAGCGGTGGTATGATCGGTGGCAGTTGTGGCGGCGGTGGTGATGACGTCGGTACATCCACCGGCACACGTTACCCCGGTAAAGAAGTTGACTTTCCTGTGATGGATTTGGCTGATGCTATGTTTAATTCAGGCAGCAGCAGCAGCAGTATGGATTTTATTTTCAGTTCTGCTGAACGTACATGGGAGCCAGATGACAAGAAAAATTGA

Protein Analysis

343

Amino Acids

37.44

Weight (kDa)

5.48

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 168 - 228 1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 870
AccB7I CCANNNNNTGG 2 cut(s) 506, 628
AccII CGCG 1 cut(s) 424
AciI CCGC 7 cut(s) 254, 318, 344, 422, 825, 852, 855
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 2 cut(s) 75, 606
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 4 cut(s) 596, 662, 874, 1000
AfiI CCNNNNNNNGG 6 cut(s) 337, 479, 502, 506, 628, 809
AflIII ACRYGT 2 cut(s) 684, 887
AgsI TTSAA 6 cut(s) 100, 168, 233, 394, 653, 705
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 4 cut(s) 52, 290, 383, 411
AluI AGCT 4 cut(s) 52, 290, 383, 411
Alw21I GWGCWC 1 cut(s) 210
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 3 cut(s) 386, 628, 695
AoxI GGCC 2 cut(s) 75, 606
ApeKI GCWGC 9 cut(s) 287, 752, 816, 819, 822, 954, 957, 960, 963
AspA2I CCTAGG 1 cut(s) 473
AsuC2I CCSGG 2 cut(s) 332, 897
AsuHPI GGTGA 2 cut(s) 680, 872
AvrII CCTAGG 1 cut(s) 473
BalI TGGCCA 2 cut(s) 77, 608
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 9 cut(s) 299, 764, 828, 831, 834, 966, 969, 972, 975
BccI CCATC 5 cut(s) 500, 591, 742, 774, 916
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 2 cut(s) 332, 897
BfaI CTAG 3 cut(s) 45, 284, 474
BfmI CTRYAG 1 cut(s) 571
BlnI CCTAGG 1 cut(s) 473
Bme1390I CCNGG 3 cut(s) 332, 605, 897
BmiI GGNNCC 1 cut(s) 1008
BmrFI CCNGG 3 cut(s) 332, 605, 897
BmsI GCATC 3 cut(s) 23, 566, 925
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 332, 897
BsaHI GRCGYC 1 cut(s) 867
BsaJI CCNNGG 6 cut(s) 28, 331, 473, 560, 789, 895
BsaXI ACNNNNNCTCC 2 cut(s) 751, 781
Bsc4I CCNNNNNNNGG 6 cut(s) 337, 479, 502, 506, 628, 809
Bse118I RCCGGY 1 cut(s) 881
Bse3DI GCAATG 2 cut(s) 267, 396
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 6 cut(s) 28, 331, 473, 560, 789, 895
BseGI GGATG 5 cut(s) 10, 38, 376, 511, 875
BseLI CCNNNNNNNGG 6 cut(s) 337, 479, 502, 506, 628, 809
BseMI GCAATG 2 cut(s) 267, 396
BseMII CTCAG 3 cut(s) 233, 374, 753
BseXI GCAGC 9 cut(s) 299, 764, 828, 831, 834, 966, 969, 972, 975
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 77, 608
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 331, 882, 897
BslI CCNNNNNNNGG 6 cut(s) 337, 479, 502, 506, 628, 809
BsnI GGCC 2 cut(s) 77, 608
Bsp1286I GDGCHC 1 cut(s) 210
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
Bsp19I CCATGG 2 cut(s) 28, 789
BspACI CCGC 7 cut(s) 254, 318, 344, 422, 825, 852, 855
BspANI GGCC 2 cut(s) 77, 608
BspCNI CTCAG 3 cut(s) 234, 373, 754
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 1008
BspPI GGATC 1 cut(s) 632
BsrDI GCAATG 2 cut(s) 267, 396
BsrFI RCCGGY 1 cut(s) 881
BsrGI TGTACA 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 6 cut(s) 28, 331, 473, 560, 789, 895
BssMI GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 4 cut(s) 28, 473, 560, 789
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 463
BstACI GRCGYC 1 cut(s) 867
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 3 cut(s) 258, 381, 468
BstDEI CTNAG 3 cut(s) 242, 360, 762
BstDSI CCRYGG 2 cut(s) 28, 789
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 5 cut(s) 10, 38, 376, 511, 875
BstFNI CGCG 1 cut(s) 424
BstKTI GATC 8 cut(s) 295, 312, 439, 543, 640, 727, 745, 837
BstMBI GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
BstMWI GCNNNNNNNGC 4 cut(s) 822, 849, 960, 963
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 3 cut(s) 620, 681, 688
BstSCI CCNGG 3 cut(s) 330, 603, 895
BstSFI CTRYAG 1 cut(s) 571
BstUI CGCG 1 cut(s) 424
BstV1I GCAGC 9 cut(s) 299, 764, 828, 831, 834, 966, 969, 972, 975
BstXI CCANNNNNNTGG 1 cut(s) 218
BsuRI GGCC 2 cut(s) 77, 608
BtgI CCRYGG 2 cut(s) 28, 789
BtsCI GGATG 5 cut(s) 10, 38, 376, 511, 875
BtsI GCAGTG 2 cut(s) 372, 693
BtsIMutI CAGTG 6 cut(s) 319, 372, 468, 486, 693, 759
Cac8I GCNNGC 3 cut(s) 258, 381, 468
CaiI CAGNNNCTG 3 cut(s) 386, 628, 695
Cfr10I RCCGGY 1 cut(s) 881
Csp6I GTAC 4 cut(s) 595, 661, 873, 999
CviAII CATG 6 cut(s) 29, 617, 678, 685, 790, 1002
CviQI GTAC 4 cut(s) 595, 661, 873, 999
DdeI CTNAG 3 cut(s) 242, 360, 762
DpnI GATC 8 cut(s) 294, 311, 438, 542, 639, 726, 744, 836
DpnII GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
EaeI YGGCCR 2 cut(s) 75, 606
Eco130I CCWWGG 4 cut(s) 28, 473, 560, 789
Eco32I GATATC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 4 cut(s) 28, 473, 560, 789
ErhI CCWWGG 4 cut(s) 28, 473, 560, 789
FaeI CATG 6 cut(s) 32, 620, 681, 688, 793, 1005
FatI CATG 6 cut(s) 28, 616, 677, 684, 789, 1001
FokI GGATG 5 cut(s) 17, 45, 383, 518, 862
FspBI CTAG 3 cut(s) 45, 284, 474
HaeIII GGCC 2 cut(s) 77, 608
HapII CCGG 3 cut(s) 331, 882, 897
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 6 cut(s) 32, 620, 681, 688, 793, 1005
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 3 cut(s) 89, 164, 555
HpaII CCGG 3 cut(s) 331, 882, 897
HphI GGTGA 2 cut(s) 680, 872
Hpy166II GTNNAC 2 cut(s) 671, 910
Hpy188I TCNGA 2 cut(s) 388, 747
Hpy188III TCNNGA 3 cut(s) 161, 622, 777
Hpy8I GTNNAC 2 cut(s) 671, 910
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 1 cut(s) 711
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 4 cut(s) 659, 867, 889, 997
HpyCH4V TGCA 2 cut(s) 579, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 822, 849, 960, 963
HpyF3I CTNAG 3 cut(s) 242, 360, 762
HpySE526I ACGT 4 cut(s) 659, 867, 889, 997
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 6 cut(s) 32, 620, 681, 688, 793, 1005
Kzo9I GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
LmnI GCTCC 1 cut(s) 1006
Lsp1109I GCAGC 9 cut(s) 299, 764, 828, 831, 834, 966, 969, 972, 975
LweI GCATC 3 cut(s) 23, 566, 925
MaeI CTAG 3 cut(s) 45, 284, 474
MaeII ACGT 4 cut(s) 659, 867, 889, 997
MaeIII GTNAC 3 cut(s) 566, 655, 890
MalI GATC 8 cut(s) 294, 311, 438, 542, 639, 726, 744, 836
MboI GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
MboII GAAGA 2 cut(s) 176, 319
MfeI CAATTG 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 2 cut(s) 77, 608
MluCI AATT 6 cut(s) 100, 447, 598, 705, 946, 1024
MluNI TGGCCA 2 cut(s) 77, 608
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 794
MnlI CCTC 8 cut(s) 35, 237, 334, 496, 618, 652, 777, 803
Mox20I TGGCCA 2 cut(s) 77, 608
MscI TGGCCA 2 cut(s) 77, 608
MseI TTAA 5 cut(s) 20, 197, 446, 798, 945
MslI CAYNNNNRTG 1 cut(s) 778
Msp20I TGGCCA 2 cut(s) 77, 608
MspA1I CMGCKG 2 cut(s) 290, 825
MspI CCGG 3 cut(s) 331, 882, 897
MspR9I CCNGG 3 cut(s) 332, 605, 897
MunI CAATTG 1 cut(s) 598
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 4 cut(s) 822, 849, 960, 963
NciI CCSGG 2 cut(s) 332, 897
NcoI CCATGG 2 cut(s) 28, 789
NdeII GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
NlaIII CATG 6 cut(s) 32, 620, 681, 688, 793, 1005
NlaIV GGNNCC 1 cut(s) 1008
NspI RCATGY 3 cut(s) 620, 681, 688
PciI ACATGT 1 cut(s) 684
PfeI GAWTC 2 cut(s) 164, 555
PflMI CCANNNNNTGG 2 cut(s) 506, 628
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PscI ACATGT 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 1008
PstNI CAGNNNCTG 3 cut(s) 386, 628, 695
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 4 cut(s) 596, 662, 874, 1000
RsaNI GTAC 4 cut(s) 595, 661, 873, 999
RseI CAYNNNNRTG 1 cut(s) 778
SaqAI TTAA 5 cut(s) 20, 197, 446, 798, 945
Sau3AI GATC 8 cut(s) 292, 309, 436, 540, 637, 724, 742, 834
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 332, 605, 897
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 3 cut(s) 23, 566, 925
SfcI CTRYAG 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 778
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 6 cut(s) 100, 447, 598, 705, 946, 1024
SsiI CCGC 7 cut(s) 254, 318, 344, 422, 825, 852, 855
SspMI CTAG 3 cut(s) 45, 284, 474
StyD4I CCNGG 3 cut(s) 330, 603, 895
StyI CCWWGG 4 cut(s) 28, 473, 560, 789
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 4 cut(s) 662, 870, 892, 1000
TaqI TCGA 3 cut(s) 295, 644, 778
TasI AATT 6 cut(s) 100, 447, 598, 705, 946, 1024
TatI WGTACW 1 cut(s) 594
TauI GCSGC 3 cut(s) 321, 347, 855
TfiI GAWTC 2 cut(s) 164, 555
Tru1I TTAA 5 cut(s) 20, 197, 446, 798, 945
Tru9I TTAA 5 cut(s) 20, 197, 446, 798, 945
TscAI CASTG 6 cut(s) 319, 372, 468, 493, 700, 766
TseI GCWGC 9 cut(s) 287, 752, 816, 819, 822, 954, 957, 960, 963
TspDTI ATGAA 3 cut(s) 93, 168, 393
TspRI CASTG 6 cut(s) 319, 372, 468, 493, 700, 766
Van91I CCANNNNNTGG 2 cut(s) 506, 628
XagI CCTNNNNNAGG 1 cut(s) 807
XceI RCATGY 3 cut(s) 620, 681, 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmaJI CCTAGG 1 cut(s) 473
XspI CTAG 3 cut(s) 45, 284, 474
ZraI GACGTC 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.