Rw1G033440
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010870
Physical Location & Seq
Forward (+)
61521429 .. 61523713
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033440.1

Sequence Viewer

Length: 1149 bp
ATGGGGCACCAGCACAGAAGCCTCACCACAGCTTACATTAGCTACCCTTTTAAATTTCACCACAACATTTTCAAAAATACGACAAGCCAAACCAAGCAAACCATGGATAACATTAGTGAAACCGTTTCTTTAATTCACCGTGGTTGCAACTTGGCTAGAGACCTGGAATCAAACCTTCCCAACTTGCCAAGCCGACCCGAAACCGTTTCGAAATCCTTGGACGAGATAATCAGGGTTTTCGGTGCAGCAAGGGAGAGGCTGCAGAGTAGTAGTGCTGCACAACATGATCCAATGAGTTCATACGTTCATGTAGCGCAGCAGCAGCAGCAGATCGATGCGAGCTTGCAGGAATGGCTGAGGTGTAATTACACACAGGCAATGGAGGATCTTCACATCCAAACGCAGCGTTTTGCATCCGAAAAGAGTATTGCTGCTACTCAATCCGATATGAGGGATTCAGGAGCTGACGTACTTCAAGGCCAAATCCATCAGGCAATCGTTTCTCATGAACTGAATGCTGCTTCGTCCTCACAAAGCAGACCACGAAGAAGGAGGGACGATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCGGATCGGGAATACTGAAATCCCACCGGAGGATGGCTTCACGTGGCGAAAATACGGCCAGAAGGAGATCATGGGCTGCAGGTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATGTACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAAGTCTACCACCATCTGATCAAATTAAGCAAGAGACTATGACCCAAACTCATGCGGCAACCACTCAACCCTTAGGAACATGGCTCTCCATGGACTTCAACGCGAGAGGTGGAGGAAGCAGCAGTGGTGGCCGCGGTGATGGTGACGGTGACGGTGTAGGTACATCAATCACGACGACCACTCGTTACGGTAAAGACGTTGAGTTTCCGGTGGTGGATTTGGCTGATGCAATGTTTAATTCAGGGAGTAGCAGCAGTAATAGTATGGATTTTATGTTCACTTCATCTATGGACAGTAAATGGGAGTCCGGGGACAAGAAAAATTGA

Protein Analysis

382

Amino Acids

42.64

Weight (kDa)

6.92

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 208 - 268 3.4e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 663
AccB1I GGYRCC 2 cut(s) 6, 589
AccI GTMKAC 1 cut(s) 829
AccII CGCG 2 cut(s) 926, 957
AciI CCGC 3 cut(s) 878, 955, 957
AclWI GGATC 4 cut(s) 281, 393, 605, 752
AcoI YGGCCR 2 cut(s) 649, 952
AcsI RAATTY 2 cut(s) 53, 567
AcvI CACGTG 1 cut(s) 636
AfaI GTAC 4 cut(s) 471, 700, 782, 985
AfiI CCNNNNNNNGG 4 cut(s) 450, 599, 622, 626
AflIII ACRYGT 1 cut(s) 797
AgsI TTSAA 4 cut(s) 73, 476, 773, 922
AjnI CCWGG 1 cut(s) 162
AloI GAACNNNNNNTCC 2 cut(s) 1082, 1114
AluBI AGCT 4 cut(s) 32, 42, 342, 464
AluI AGCT 4 cut(s) 32, 42, 342, 464
Alw26I GTCTC 2 cut(s) 153, 851
AlwI GGATC 4 cut(s) 281, 393, 605, 752
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 3 cut(s) 478, 649, 952
ApoI RAATTY 2 cut(s) 53, 567
AspLEI GCGC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 1132
AsuHPI GGTGA 7 cut(s) 16, 50, 128, 800, 971, 977, 983
AsuII TTCGAA 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 895
BaeGI GKGCMC 1 cut(s) 9
BanI GGYRCC 2 cut(s) 6, 589
BbrPI CACGTG 1 cut(s) 636
BbvCI CCTCAGC 1 cut(s) 356
BccI CCATC 5 cut(s) 495, 554, 620, 844, 956
BceAI ACGGC 1 cut(s) 664
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 1 cut(s) 1132
BcoDI GTCTC 2 cut(s) 153, 851
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 3 cut(s) 260, 670, 691
BfuAI ACCTGC 1 cut(s) 663
Bme1390I CCNGG 2 cut(s) 164, 1132
BmiI GGNNCC 3 cut(s) 8, 591, 677
BmrFI CCNGG 2 cut(s) 164, 1132
BmsI GCATC 3 cut(s) 325, 422, 1039
Bpu10I CCTNAGC 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 808
BpuMI CCSGG 1 cut(s) 1132
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 8 cut(s) 102, 139, 216, 593, 680, 912, 955, 1131
BsaWI WCCGGW 2 cut(s) 619, 1030
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 450, 599, 622, 626
Bse118I RCCGGY 1 cut(s) 586
Bse21I CCTNAGG 1 cut(s) 895
Bse3DI GCAATG 2 cut(s) 384, 1059
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 8 cut(s) 102, 139, 216, 593, 680, 912, 955, 1131
BseGI GGATG 3 cut(s) 393, 413, 631
BseLI CCNNNNNNNGG 4 cut(s) 450, 599, 622, 626
BseMI GCAATG 2 cut(s) 384, 1059
BseMII CTCAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 9
BseYI CCCAGC 1 cut(s) 724
BsgI GTGCAG 3 cut(s) 261, 264, 801
Bsh1236I CGCG 2 cut(s) 926, 957
BshFI GGCC 3 cut(s) 480, 651, 954
BshNI GGYRCC 2 cut(s) 6, 589
BshVI ATCGAT 1 cut(s) 333
BsiSI CCGG 4 cut(s) 587, 620, 1031, 1131
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 4 cut(s) 450, 599, 622, 626
BsmAI GTCTC 2 cut(s) 153, 851
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 3 cut(s) 480, 651, 954
Bso31I GGTCTC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
Bsp19I CCATGG 2 cut(s) 102, 912
BspACI CCGC 3 cut(s) 878, 955, 957
BspANI GGCC 3 cut(s) 480, 651, 954
BspCNI CTCAG 1 cut(s) 348
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 2 cut(s) 926, 957
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 3 cut(s) 8, 591, 677
BspMAI CTGCAG 2 cut(s) 264, 674
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 4 cut(s) 281, 393, 605, 752
BspT104I TTCGAA 1 cut(s) 209
BspT107I GGYRCC 2 cut(s) 6, 589
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 2 cut(s) 384, 1059
BsrFI RCCGGY 1 cut(s) 586
BsrGI TGTACA 1 cut(s) 780
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 8 cut(s) 102, 139, 216, 593, 680, 912, 955, 1131
BssMI GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
BssT1I CCWWGG 4 cut(s) 102, 216, 680, 912
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 8 cut(s) 124, 140, 205, 583, 971, 977, 1013, 1118
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 1 cut(s) 636
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 340, 344, 588
BstDEI CTNAG 2 cut(s) 356, 895
BstDSI CCRYGG 4 cut(s) 102, 139, 912, 955
BstEII GGTNACC 1 cut(s) 788
BstF5I GGATG 3 cut(s) 393, 413, 631
BstFNI CGCG 2 cut(s) 926, 957
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 7 cut(s) 289, 333, 388, 600, 663, 760, 844
BstMAI GTCTC 2 cut(s) 153, 851
BstMBI GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
BstMWI GCNNNNNNNGC 4 cut(s) 322, 325, 352, 951
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 801
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 2 cut(s) 162, 1130
BstSFI CTRYAG 3 cut(s) 260, 670, 691
BstSLI GKGCMC 1 cut(s) 9
BstUI CGCG 2 cut(s) 926, 957
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 333
Bsu36I CCTNAGG 1 cut(s) 895
BsuRI GGCC 3 cut(s) 480, 651, 954
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 4 cut(s) 102, 139, 912, 955
BtsCI GGATG 3 cut(s) 393, 413, 631
BtsI GCAGTG 2 cut(s) 813, 952
BtsIMutI CAGTG 2 cut(s) 813, 952
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 340, 344, 588
CaiI CAGNNNCTG 1 cut(s) 464
CciI TCATGA 1 cut(s) 505
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 586
Cfr42I CCGCGG 1 cut(s) 958
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 4 cut(s) 470, 699, 781, 984
CviAII CATG 9 cut(s) 103, 284, 308, 506, 664, 798, 875, 903, 913
CviQI GTAC 4 cut(s) 470, 699, 781, 984
DdeI CTNAG 2 cut(s) 356, 895
DpnI GATC 7 cut(s) 288, 332, 387, 599, 662, 759, 843
DpnII GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 2 cut(s) 649, 952
Eco130I CCWWGG 4 cut(s) 102, 216, 680, 912
Eco31I GGTCTC 1 cut(s) 153
Eco72I CACGTG 1 cut(s) 636
Eco81I CCTNAGG 1 cut(s) 895
Eco91I GGTNACC 1 cut(s) 788
EcoO65I GGTNACC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 4 cut(s) 102, 216, 680, 912
ErhI CCWWGG 4 cut(s) 102, 216, 680, 912
FaeI CATG 9 cut(s) 106, 287, 311, 509, 667, 801, 878, 906, 916
FaqI GGGAC 1 cut(s) 569
FatI CATG 9 cut(s) 102, 283, 307, 505, 663, 797, 874, 902, 912
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 829
FokI GGATG 3 cut(s) 380, 400, 638
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 315
GsaI CCCAGC 1 cut(s) 728
HaeIII GGCC 3 cut(s) 480, 651, 954
HapII CCGG 4 cut(s) 587, 620, 1031, 1131
HhaI GCGC 1 cut(s) 316
Hin1II CATG 9 cut(s) 106, 287, 311, 509, 667, 801, 878, 906, 916
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HinfI GANTC 3 cut(s) 167, 455, 1127
HpaII CCGG 4 cut(s) 587, 620, 1031, 1131
HphI GGTGA 7 cut(s) 16, 50, 128, 800, 971, 977, 983
Hpy166II GTNNAC 4 cut(s) 699, 813, 830, 1101
Hpy188I TCNGA 5 cut(s) 418, 445, 597, 744, 841
Hpy188III TCNNGA 4 cut(s) 459, 506, 601, 994
Hpy8I GTNNAC 4 cut(s) 699, 813, 830, 1101
Hpy99I CGWCG 1 cut(s) 1000
HpyAV CCTTC 3 cut(s) 185, 543, 649
HpyCH4III ACNGT 8 cut(s) 124, 140, 205, 583, 971, 977, 1013, 1118
HpyCH4IV ACGT 4 cut(s) 303, 468, 635, 1020
HpyCH4V TGCA 9 cut(s) 147, 245, 262, 278, 346, 413, 672, 818, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 322, 325, 352, 951
HpyF3I CTNAG 2 cut(s) 356, 895
HpySE526I ACGT 4 cut(s) 303, 468, 635, 1020
Hsp92II CATG 9 cut(s) 106, 287, 311, 509, 667, 801, 878, 906, 916
HspAI GCGC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 841
KspI CCGCGG 1 cut(s) 958
Kzo9I GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
LmnI GCTCC 1 cut(s) 461
LweI GCATC 3 cut(s) 325, 422, 1039
MaeI CTAG 1 cut(s) 156
MaeII ACGT 4 cut(s) 303, 468, 635, 1020
MaeIII GTNAC 4 cut(s) 788, 965, 971, 1007
MalI GATC 7 cut(s) 288, 332, 387, 599, 662, 759, 843
MboI GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
MboII GAAGA 2 cut(s) 380, 558
MfeI CAATTG 1 cut(s) 718
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 9 cut(s) 53, 132, 364, 567, 575, 718, 846, 1060, 1144
MlyI GAGTC 1 cut(s) 1136
MroNI GCCGGC 1 cut(s) 586
MseI TTAA 5 cut(s) 51, 131, 764, 849, 1059
MspA1I CMGCKG 1 cut(s) 957
MspI CCGG 4 cut(s) 587, 620, 1031, 1131
MspR9I CCNGG 2 cut(s) 164, 1132
MunI CAATTG 1 cut(s) 718
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 926, 957
MwoI GCNNNNNNNGC 4 cut(s) 322, 325, 352, 951
NaeI GCCGGC 1 cut(s) 588
NciI CCSGG 1 cut(s) 1132
NcoI CCATGG 2 cut(s) 102, 912
NdeI CATATG 1 cut(s) 804
NdeII GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 9 cut(s) 106, 287, 311, 509, 667, 801, 878, 906, 916
NlaIV GGNNCC 3 cut(s) 8, 591, 677
NmuCI GTSAC 3 cut(s) 788, 965, 971
NspI RCATGY 1 cut(s) 801
NspV TTCGAA 1 cut(s) 209
PagI TCATGA 1 cut(s) 505
PciI ACATGT 1 cut(s) 797
PcsI WCGNNNNNNNCGW 1 cut(s) 1017
PctI GAATGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 167, 455
PleI GAGTC 1 cut(s) 1135
PmaCI CACGTG 1 cut(s) 636
PmlI CACGTG 1 cut(s) 636
PpsI GAGTC 1 cut(s) 1135
Ppu21I YACGTR 1 cut(s) 636
PscI ACATGT 1 cut(s) 797
Psp6I CCWGG 1 cut(s) 162
PspCI CACGTG 1 cut(s) 636
PspEI GGTNACC 1 cut(s) 788
PspFI CCCAGC 1 cut(s) 724
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 3 cut(s) 8, 591, 677
PstI CTGCAG 2 cut(s) 264, 674
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 4 cut(s) 471, 700, 782, 985
RsaNI GTAC 4 cut(s) 470, 699, 781, 984
SacII CCGCGG 1 cut(s) 958
SaqAI TTAA 5 cut(s) 51, 131, 764, 849, 1059
Sau3AI GATC 7 cut(s) 286, 330, 385, 597, 660, 757, 841
SchI GAGTC 1 cut(s) 1136
ScrFI CCNGG 2 cut(s) 164, 1132
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 3 cut(s) 325, 422, 1039
SfcI CTRYAG 3 cut(s) 260, 670, 691
Sfr303I CCGCGG 1 cut(s) 958
SfuI TTCGAA 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 958
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 9 cut(s) 53, 132, 364, 567, 575, 718, 846, 1060, 1144
SsiI CCGC 3 cut(s) 878, 955, 957
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 162, 1130
StyI CCWWGG 4 cut(s) 102, 216, 680, 912
TaaI ACNGT 8 cut(s) 124, 140, 205, 583, 971, 977, 1013, 1118
TaiI ACGT 4 cut(s) 306, 471, 638, 1023
TaqI TCGA 2 cut(s) 209, 333
TasI AATT 9 cut(s) 53, 132, 364, 567, 575, 718, 846, 1060, 1144
TatI WGTACW 1 cut(s) 780
TauI GCSGC 2 cut(s) 881, 957
TfiI GAWTC 2 cut(s) 167, 455
Tru1I TTAA 5 cut(s) 51, 131, 764, 849, 1059
Tru9I TTAA 5 cut(s) 51, 131, 764, 849, 1059
TscAI CASTG 2 cut(s) 820, 952
TseFI GTSAC 3 cut(s) 788, 965, 971
Tsp45I GTSAC 3 cut(s) 788, 965, 971
TspDTI ATGAA 4 cut(s) 288, 296, 522, 1095
TspRI CASTG 2 cut(s) 820, 952
XapI RAATTY 2 cut(s) 53, 567
XceI RCATGY 1 cut(s) 801
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 829
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.