FvH4_7g33593

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006536
Physical Location & Seq
Reverse (-)
23864889 .. 23868539
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33593.t1

Sequence Viewer

Length: 1515 bp
ATGGACAAGACTCGTTCCGGTGATGTTGACCATTCTGATCATGAGCAATTTTCTGAGCTTTTGGAAGTGCCGATTGAGTATCTGAAAACAGTTCAATGGAAGTTGGTTGTTGAATTAGAAGAAATTAAATTTGTGAAGGGGACGGAAAAGTTCTGTGTTTCCGAATCTTATGAACTTGATCAAGAGAAATTGAAGGCCGTGAAGACTCTTAAGGCTCGACGAGTTTATTTCCTCAAGGAGGATGACGTTTTCTTCTTGGCTTTTCTGTGGATGGCAACGATGAATACACCATTGGAGGGAATAGAAAAGTTTGCACACTGTTTCGCATCTCCATTAATCGAGTCTACAGAAAAATTATTTTATGGGGAGGAAAGTGATATTCCTATGCTGTTTGATTTGGCTGGTACACAACTCGAGAATATTAAAGCTTACAATAGCAAAATATTAGATTTTCTGGAAGCTGATGACTTGAACCAGGTAACCTTGCAGGAGTTGAGCAATCACTACTTAAGTTTGGATAAAGGAATATGGAGTGTGAATATGAAAATTGACATTCTGAGATTTCACTTGGCGCACCGACGACTTGATCTAAAGGATTTGGTGGACAGATTTGTGGAATCGGTGGAAGATGAACTCCAGCGATATGAGGAGGAACTAGAGAAGACCAGGTTTAAAGTGCTTCAATTAGTGAAGAAGCACAAAACCAACATGTTTGGACTTCGATGGTTACCTGATTCTTGGAAGGGAACTTTCGTGTCTATTGGCATGAAGACAATGGTCTTTGGTGCGAGACTGATGCAGTGTCTTGGCTGGCATGAATACCTGGCGTCATACGGTTGGATACGGCGGGAAACGGTCAGATACGCTTTGGATACAGATGGATACGTAGCTGACTCGAGAACCCCTGATCGGAAGATACAACTCAACTCAACTCAACAGATGAATTGGACAAGTAGCTGGCGTTGCCTCACTAAGGTTTACAAAGCAGCAGATTCCAAATGTGGCTCATCCTTGTGGTCTCGGCAATTTGGAGACAAGGCTAAGGTTCCTGCTATTGAGCCTGAGCTGAATAGGGACTTCCTTGTAAAGCTATGGATGGCGGATGAGGAAATGAAGCGGTTTCGTAGAAAATCGAGGCGGAGGGCTGTTGAGCCGGAGGAGGGTAGAATGGTTGTGAAGCAGCCACCTGTGAGCCAATCCATCTCTGGATATCTGAAGCCGGAGTCTCCTGAAGAGGTCCAGGTGATGCCTCTTCTTGCTAGATCAAATATGCTTGTTACTAGGGATATAGAGTGGGCAAATCTTATGCTTGGCTTTGAGCAGGAAAACCGGTATGCCATAGTAGATGTATACTATCCACAATCGCCTGTAGGTTTCATTCGTGAAGAGAGTAATATTATCACTAGACAGCTACTTCGCCAGCGGCGTCCTTTTGTTGCTCGAGTAACTGATGGCATGGGTAATGAGCTCTTTAGGGTTCGCAGGCCCTTTTGGTGGATAAACAGCTCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

505

Amino Acids

59.34

Weight (kDa)

6.52

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 407 - 501 4.2e-27 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 344, 1350
AciI CCGC 5 cut(s) 847, 1100, 1117, 1138, 1423
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 2 cut(s) 1235, 1251
AcyI GRCGYC 2 cut(s) 827, 1426
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 6 cut(s) 238, 296, 909, 973, 1160, 1494
AflII CTTAAG 2 cut(s) 209, 508
AflIII ACRYGT 1 cut(s) 708
AgeI ACCGGT 1 cut(s) 1329
AgsI TTSAA 5 cut(s) 95, 113, 193, 472, 683
AjnI CCWGG 4 cut(s) 474, 665, 822, 1239
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
Alw21I GWGCWC 1 cut(s) 1470
Alw26I GTCTC 4 cut(s) 784, 1023, 1026, 1230
Ama87I CYCGRG 3 cut(s) 413, 895, 1440
AoxI GGCC 2 cut(s) 195, 1484
ApeKI GCWGC 2 cut(s) 986, 1180
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 1398, 1430
AseI ATTAAT 1 cut(s) 335
AsiGI ACCGGT 1 cut(s) 1329
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 2 cut(s) 1237, 1485
AsuHPI GGTGA 2 cut(s) 32, 1255
AvaI CYCGRG 3 cut(s) 413, 895, 1440
AvaII GGWCC 1 cut(s) 1237
BanII GRGCYC 1 cut(s) 1470
BbsI GAAGAC 3 cut(s) 209, 668, 776
Bbv12I GWGCWC 1 cut(s) 1470
BbvI GCAGC 2 cut(s) 998, 1192
BccI CCATC 6 cut(s) 265, 717, 872, 1090, 1208, 1445
BceAI ACGGC 2 cut(s) 182, 860
BcgI CGANNNNNNTGC 2 cut(s) 778, 812
BciT130I CCWGG 4 cut(s) 476, 667, 824, 1241
BciVI GTATCC 3 cut(s) 834, 865, 875
BclI TGATCA 2 cut(s) 37, 178
BcoDI GTCTC 4 cut(s) 784, 1023, 1026, 1230
BfaI CTAG 4 cut(s) 656, 1260, 1281, 1404
BfmI CTRYAG 2 cut(s) 345, 1368
BfrI CTTAAG 2 cut(s) 209, 508
BfuI GTATCC 3 cut(s) 834, 865, 875
BisI GCNGC 3 cut(s) 987, 1181, 1424
BlsI GCNGC 3 cut(s) 988, 1182, 1425
Bme1390I CCNGG 4 cut(s) 476, 667, 824, 1241
Bme18I GGWCC 1 cut(s) 1237
BmeT110I CYCGRG 3 cut(s) 413, 895, 1440
BmgT120I GGNCC 2 cut(s) 1237, 1485
BmiI GGNNCC 1 cut(s) 1047
BmrFI CCNGG 4 cut(s) 476, 667, 824, 1241
BmsI GCATC 3 cut(s) 335, 786, 1236
BoxI GACNNNNGTC 1 cut(s) 776
BpiI GAAGAC 3 cut(s) 209, 668, 776
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 2 cut(s) 1041, 1062
BpuEI CTTGAG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 886
BsaHI GRCGYC 2 cut(s) 827, 1426
BsaI GGTCTC 1 cut(s) 1023
BsaWI WCCGGW 2 cut(s) 17, 1329
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 296, 909, 973, 1160, 1494
Bse118I RCCGGY 1 cut(s) 1329
BseBI CCWGG 4 cut(s) 476, 667, 824, 1241
BseGI GGATG 5 cut(s) 247, 276, 1007, 1101, 1108
BseLI CCNNNNNNNGG 6 cut(s) 238, 296, 909, 973, 1160, 1494
BseMII CTCAG 3 cut(s) 45, 548, 1053
BseRI GAGGAG 2 cut(s) 662, 1172
BseXI GCAGC 2 cut(s) 998, 1192
BshFI GGCC 2 cut(s) 197, 1486
BshTI ACCGGT 1 cut(s) 1329
BsiHKAI GWGCWC 1 cut(s) 1470
BsiHKCI CYCGRG 3 cut(s) 413, 895, 1440
BsiSI CCGG 4 cut(s) 18, 1154, 1220, 1330
BslFI GGGAC 2 cut(s) 154, 1088
BslI CCNNNNNNNGG 6 cut(s) 238, 296, 909, 973, 1160, 1494
BsmAI GTCTC 4 cut(s) 784, 1023, 1026, 1230
BsmFI GGGAC 2 cut(s) 154, 1088
BsnI GGCC 2 cut(s) 197, 1486
Bso31I GGTCTC 1 cut(s) 1023
BsoBI CYCGRG 3 cut(s) 413, 895, 1440
Bsp1286I GDGCHC 1 cut(s) 1470
Bsp143I GATC 5 cut(s) 37, 178, 586, 907, 1262
BspACI CCGC 5 cut(s) 847, 1100, 1117, 1138, 1423
BspANI GGCC 2 cut(s) 197, 1486
BspCNI CTCAG 3 cut(s) 46, 549, 1054
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 1047
BspTI CTTAAG 2 cut(s) 209, 508
BspTNI GGTCTC 1 cut(s) 1023
BsrFI RCCGGY 1 cut(s) 1329
BssAI RCCGGY 1 cut(s) 1329
BssMI GATC 5 cut(s) 37, 178, 586, 907, 1262
BssNAI GTATAC 1 cut(s) 1351
BssNI GRCGYC 2 cut(s) 827, 1426
Bst1107I GTATAC 1 cut(s) 1351
Bst2UI CCWGG 4 cut(s) 476, 667, 824, 1241
Bst4CI ACNGT 4 cut(s) 91, 320, 836, 856
Bst6I CTCTTC 3 cut(s) 1227, 1257, 1380
BstACI GRCGYC 2 cut(s) 827, 1426
BstAFI CTTAAG 2 cut(s) 209, 508
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 4 cut(s) 812, 959, 1421, 1484
BstDEI CTNAG 5 cut(s) 54, 557, 972, 1041, 1062
BstEII GGTNACC 2 cut(s) 478, 726
BstENI CCTNNNNNAGG 2 cut(s) 236, 971
BstF5I GGATG 5 cut(s) 247, 276, 1007, 1101, 1108
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 5 cut(s) 40, 181, 589, 910, 1265
BstMAI GTCTC 4 cut(s) 784, 1023, 1026, 1230
BstMBI GATC 5 cut(s) 37, 178, 586, 907, 1262
BstMWI GCNNNNNNNGC 1 cut(s) 963
BstNI CCWGG 4 cut(s) 476, 667, 824, 1241
BstNSI RCATGY 1 cut(s) 712
BstPAI GACNNNNGTC 1 cut(s) 776
BstPI GGTNACC 2 cut(s) 478, 726
BstSCI CCNGG 4 cut(s) 474, 665, 822, 1239
BstSFI CTRYAG 2 cut(s) 345, 1368
BstSNI TACGTA 1 cut(s) 886
BstV1I GCAGC 2 cut(s) 998, 1192
BstV2I GAAGAC 3 cut(s) 209, 668, 776
BstZ17I GTATAC 1 cut(s) 1351
BsuI GTATCC 3 cut(s) 834, 865, 875
BsuRI GGCC 2 cut(s) 197, 1486
BtsCI GGATG 5 cut(s) 247, 276, 1007, 1101, 1108
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 2 cut(s) 316, 806
Cac8I GCNNGC 4 cut(s) 812, 959, 1421, 1484
CciI TCATGA 1 cut(s) 40
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 1329
Cfr13I GGNCC 2 cut(s) 1237, 1485
CseI GACGC 2 cut(s) 816, 1415
CsiI ACCWGGT 2 cut(s) 474, 665
Csp6I GTAC 1 cut(s) 405
CspAI ACCGGT 1 cut(s) 1329
CviAII CATG 5 cut(s) 41, 709, 766, 815, 1456
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 5 cut(s) 54, 557, 972, 1041, 1062
DpnI GATC 5 cut(s) 39, 180, 588, 909, 1264
DpnII GATC 5 cut(s) 37, 178, 586, 907, 1262
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 3 cut(s) 1227, 1257, 1380
EarI CTCTTC 3 cut(s) 1227, 1257, 1380
EciI GGCGGA 2 cut(s) 1115, 1153
Ecl136II GAGCTC 1 cut(s) 1468
Eco105I TACGTA 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 1470
Eco31I GGTCTC 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 1211
Eco47I GGWCC 1 cut(s) 1237
Eco53kI GAGCTC 1 cut(s) 1468
Eco57I CTGAAG 2 cut(s) 1235, 1251
Eco88I CYCGRG 3 cut(s) 413, 895, 1440
Eco91I GGTNACC 2 cut(s) 478, 726
EcoICRI GAGCTC 1 cut(s) 1468
EcoNI CCTNNNNNAGG 2 cut(s) 236, 971
EcoO109I RGGNCCY 1 cut(s) 1485
EcoO65I GGTNACC 2 cut(s) 478, 726
EcoRII CCWGG 4 cut(s) 474, 665, 822, 1239
EcoRV GATATC 1 cut(s) 1211
EcoT38I GRGCYC 1 cut(s) 1470
FaeI CATG 5 cut(s) 44, 712, 769, 818, 1459
FaqI GGGAC 2 cut(s) 154, 1088
FatI CATG 5 cut(s) 40, 708, 765, 814, 1455
FauI CCCGC 1 cut(s) 840
FbaI TGATCA 2 cut(s) 37, 178
FblI GTMKAC 2 cut(s) 344, 1350
Fnu4HI GCNGC 3 cut(s) 987, 1181, 1424
FokI GGATG 5 cut(s) 254, 283, 994, 1108, 1115
FriOI GRGCYC 1 cut(s) 1470
Fsp4HI GCNGC 3 cut(s) 987, 1181, 1424
FspBI CTAG 4 cut(s) 656, 1260, 1281, 1404
GlaI GCGC 1 cut(s) 573
GluI GCNGC 3 cut(s) 987, 1181, 1424
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 2 cut(s) 197, 1486
HapII CCGG 4 cut(s) 18, 1154, 1220, 1330
HgaI GACGC 2 cut(s) 816, 1415
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 2 cut(s) 827, 1426
Hin1II CATG 5 cut(s) 44, 712, 769, 818, 1459
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 9 cut(s) 10, 164, 205, 341, 617, 734, 893, 992, 1223
HpaII CCGG 4 cut(s) 18, 1154, 1220, 1330
HphI GGTGA 2 cut(s) 32, 1255
Hpy166II GTNNAC 6 cut(s) 28, 345, 407, 604, 979, 1351
Hpy188I TCNGA 8 cut(s) 37, 55, 84, 163, 558, 860, 912, 1215
Hpy188III TCNNGA 8 cut(s) 41, 182, 415, 455, 897, 1206, 1229, 1382
Hpy8I GTNNAC 6 cut(s) 28, 345, 407, 604, 979, 1351
Hpy99I CGWCG 2 cut(s) 222, 582
HpyAV CCTTC 3 cut(s) 130, 187, 736
HpyCH4III ACNGT 4 cut(s) 91, 320, 836, 856
HpyCH4IV ACGT 2 cut(s) 246, 885
HpyCH4V TGCA 3 cut(s) 314, 487, 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 963
HpyF3I CTNAG 5 cut(s) 54, 557, 972, 1041, 1062
HpySE526I ACGT 2 cut(s) 246, 885
Hsp92I GRCGYC 2 cut(s) 827, 1426
Hsp92II CATG 5 cut(s) 44, 712, 769, 818, 1459
HspAI GCGC 1 cut(s) 572
Ksp22I TGATCA 2 cut(s) 37, 178
Kzo9I GATC 5 cut(s) 37, 178, 586, 907, 1262
Lsp1109I GCAGC 2 cut(s) 998, 1192
LweI GCATC 3 cut(s) 335, 786, 1236
MabI ACCWGGT 2 cut(s) 474, 665
MaeI CTAG 4 cut(s) 656, 1260, 1281, 1404
MaeII ACGT 2 cut(s) 246, 885
MaeIII GTNAC 4 cut(s) 478, 726, 1276, 1444
MalI GATC 5 cut(s) 39, 180, 588, 909, 1264
MboI GATC 5 cut(s) 37, 178, 586, 907, 1262
MhlI GDGCHC 1 cut(s) 1470
MlyI GAGTC 5 cut(s) 4, 199, 350, 887, 1232
MmeI TCCRAC 1 cut(s) 818
MseI TTAA 7 cut(s) 126, 210, 335, 423, 509, 672, 1513
MslI CAYNNNNRTG 1 cut(s) 1012
MspA1I CMGCKG 1 cut(s) 1423
MspCI CTTAAG 2 cut(s) 209, 508
MspI CCGG 4 cut(s) 18, 1154, 1220, 1330
MspR9I CCNGG 4 cut(s) 476, 667, 824, 1241
MvaI CCWGG 4 cut(s) 476, 667, 824, 1241
MwoI GCNNNNNNNGC 1 cut(s) 963
NdeII GATC 5 cut(s) 37, 178, 586, 907, 1262
NlaIII CATG 5 cut(s) 44, 712, 769, 818, 1459
NlaIV GGNNCC 1 cut(s) 1047
NmeAIII GCCGAG 1 cut(s) 1000
NspI RCATGY 1 cut(s) 712
PaeR7I CTCGAG 3 cut(s) 413, 895, 1440
PagI TCATGA 1 cut(s) 40
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 1423
PfeI GAWTC 4 cut(s) 164, 617, 734, 992
PinAI ACCGGT 1 cut(s) 1329
PkrI GCNGC 3 cut(s) 988, 1182, 1425
PleI GAGTC 5 cut(s) 4, 199, 349, 887, 1231
PpsI GAGTC 5 cut(s) 4, 199, 349, 887, 1231
Ppu21I YACGTR 1 cut(s) 886
PscI ACATGT 1 cut(s) 708
PshAI GACNNNNGTC 1 cut(s) 776
PshBI ATTAAT 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 1470
Psp6I CCWGG 4 cut(s) 474, 665, 822, 1239
PspEI GGTNACC 2 cut(s) 478, 726
PspGI CCWGG 4 cut(s) 474, 665, 822, 1239
PspN4I GGNNCC 1 cut(s) 1047
PspPI GGNCC 2 cut(s) 1237, 1485
PspXI VCTCGAGB 1 cut(s) 1440
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 1012
SacI GAGCTC 1 cut(s) 1470
SaqAI TTAA 7 cut(s) 126, 210, 335, 423, 509, 672, 1513
SatI GCNGC 3 cut(s) 987, 1181, 1424
Sau3AI GATC 5 cut(s) 37, 178, 586, 907, 1262
Sau96I GGNCC 2 cut(s) 1237, 1485
SchI GAGTC 5 cut(s) 4, 199, 350, 887, 1232
ScrFI CCNGG 4 cut(s) 476, 667, 824, 1241
SduI GDGCHC 1 cut(s) 1470
SexAI ACCWGGT 2 cut(s) 474, 665
SfaNI GCATC 3 cut(s) 335, 786, 1236
SfcI CTRYAG 2 cut(s) 345, 1368
Sfr274I CTCGAG 3 cut(s) 413, 895, 1440
SinI GGWCC 1 cut(s) 1237
SlaI CTCGAG 3 cut(s) 413, 895, 1440
SmiMI CAYNNNNRTG 1 cut(s) 1012
SmlI CTYRAG 6 cut(s) 209, 233, 413, 508, 895, 1440
SmoI CTYRAG 6 cut(s) 209, 233, 413, 508, 895, 1440
SnaBI TACGTA 1 cut(s) 886
SsiI CCGC 5 cut(s) 847, 1100, 1117, 1138, 1423
SspI AATATT 3 cut(s) 421, 444, 1396
SspMI CTAG 4 cut(s) 656, 1260, 1281, 1404
SstI GAGCTC 1 cut(s) 1470
StyD4I CCNGG 4 cut(s) 474, 665, 822, 1239
TaaI ACNGT 4 cut(s) 91, 320, 836, 856
TaiI ACGT 2 cut(s) 249, 888
TaqI TCGA 7 cut(s) 217, 339, 414, 721, 896, 1133, 1441
TauI GCSGC 1 cut(s) 1426
TfiI GAWTC 4 cut(s) 164, 617, 734, 992
Tru1I TTAA 7 cut(s) 126, 210, 335, 423, 509, 672, 1513
Tru9I TTAA 7 cut(s) 126, 210, 335, 423, 509, 672, 1513
TscAI CASTG 2 cut(s) 323, 806
TseI GCWGC 2 cut(s) 986, 1180
TspDTI ATGAA 9 cut(s) 186, 296, 557, 645, 782, 831, 956, 1127, 1366
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 2 cut(s) 323, 806
Vha464I CTTAAG 2 cut(s) 209, 508
VpaK11BI GGWCC 1 cut(s) 1237
VspI ATTAAT 1 cut(s) 335
XagI CCTNNNNNAGG 2 cut(s) 236, 971
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 1202
XhoI CTCGAG 3 cut(s) 413, 895, 1440
XmiI GTMKAC 2 cut(s) 344, 1350
XspI CTAG 4 cut(s) 656, 1260, 1281, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.