FvH4_7g33620

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006536
Physical Location & Seq
Reverse (-)
23871274 .. 23876440
5167 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33620.t1

Sequence Viewer

Length: 2238 bp
ATGGAGAGTACAGATGCCGGCGATTTCAGCGTCGGATCGATCGTCTGGGTGCGGCGGAGGAACGGGTCGTGGTGGCCGGGGAAGATAGTGGGGCCGGAGGAGCTCTCCACTTCGCATCTCACCTCGCCTCGCTCTGGTACTCCCGTCAAGCTTCTCGGCCGAGAAGACGCCAGTGTGGATTGGTACAATTTGGAAAAATCCAAGCGTGTTAAGCCATTTCGATGCGGTGATTTTGATGACTGCATTCACAAGGCTGAATCAGCGCAAGGTATGCCGCCAAAGAAAAGAGAAAAATATGCACGTCGGGAAGATGCTATTCTTCATGCGCTTGAACTTGAGAGGGAACTATTGAGGAAAGAAGGAAATTTGGCTATGAGTGCTGAAGTCATGAACAGACAGAACAGTAAACATGGGGCTGTGAAGAAGGATGTATTTATGCCCTCAGAAAGTCTGGGAAAAGAATATTCAAAACTTGGGAATTCCAAATTGAATCAGTTGTACAAGCGAAAAGATTTGTCTCACATGAATGATATTATAGGCAGTCCCCTGTCTTCCCAAAGACTCAAGGAGGGAAATCAACTGAGCGGGGAAGATGATCATTTTGAGGTGACACCTCGGATGAGAGGCTTGCAGGACTTTGGTCTCAAAAGTTCCTCTTCAAAGCGGAAAATTTCATCTTCCTCTTCTTTAAATGGTTTTTGGAAACCCTCTGCTGATAGTACTGTTGAAACATTTGCTCATGGTGGTCTCGGCCTGGGAGGAACAAGTCACGTGAACGGAGTGGAGCAGACAAGAGCTGTTTGCAGAGCAAAGAGGAGTAAATGTGTATACTTGCCAGCTGACTCTGGTGATTCTTTGGAGTATGAAACAGCTCCTTCAGGCCATATTGACATATCAGCTCCCCCTATTGGCACTCGTCTACATGGGGAGACATTGATAGAGGACACCACTTCTGGGTTTACAGAAGAGGAGCCTGATTCTTCTGAAACTGATTCCTCTATCCCTGGATCTGATTCCTCAGAGACTGAACCAGATATGGATGATGAGCTGCCTTCATTTTCAGATTTAGAAGTTGGAAGATATGAAGCACAAGAACATAGAAGCTTGGTTGATGAGTACCCAGATGAATCAACACATTCTGGTGACATGCCTCACCTCTATAATCAGGACCCTTATTTTGCAAGTGAAGCTGTGTCCAAGTGGCAGTTGAAAGGGAAAAGGAATACGCGCAATCTTAGTAAAAGATCAATTGATACAACCGAAGGACGAGGTTTTTTTTATGGACCATATTCTGAAGAAAAGGCTGGAATGACTGGACTGGACAATGGGTATCCCCTATCAAGAGCTGCATCAAGGAGTCGAAACAGAGTTGGACTGTATATGATTGATTGGGAGGATGGGACCTGGGAAGATAGGTCTGCCTGGGATGAATGCTGGGACAAAAGAGAGCGATATGACCAGATATATGATGATCTTTATAATCATCGCAGGAGGCCGAAATACTTGATAGATGTGGACTTGAAAGTCCAGGCAAGTTACCAGAAAGAGCCTGTCCCTATTGTTTCTCTGATGAGCAAGTTAAACGGGAAGGCGATAATAGGGCACCCCATTCAAGTTGAAGCCTTAGAAGATGGTTTATCTAACACGCTATTTTCAACATTTGATGATTCTGGTGATGAAGCAATCGACAATGATGGGGGTACAACTCTTCCTCACGCATGGAGGACTGCAAGGAGGACAGCAAATGTTCGAGTCCCTCGTCCTCATTTAGTTTCTACATTGGATGGTGATGAGGCTGCTGATGACCTTCCCTTTTCCGATGAAGATAGACCACCATTTAAGAAATTAAATATGGGGAGTTCCAGTCATAAGGCAAGCCAGGTCAAGAGGAACCCTCCTCACAACTCCCGGCTTCCCACTGATAGAAAGTTCTCGAAAAAGGTGTCTAAGAAAGTAAGCTTATCATCTAGCCTGAAAACAAAAACAAGGACCTTGTCTTCTTTAGCTATTGAACAGAGTTTTGGTAACAAGGGAATGATACAACATGGTAGTGGCCAAAGGGATCGGTTGATGAAACCAGAGTCATCTGGATCTGTGCCCACAACTGTAGCTTGCATTCCTGTGACATTAATATTCAGTAGGTTACTTGAGAAGATCAATAGGCCGCCATCAAAAGCATCAACGGCACCAGCTAGTAATGCGGTTTTATTGAATGGGGATGCAGAGAGAAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

746

Amino Acids

82.83

Weight (kDa)

6.33

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 12 - 92 8.3e-11 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1479
AasI GACNNNNNNGTC 1 cut(s) 1523
AccB1I GGYRCC 2 cut(s) 1602, 2185
AccBSI CCGCTC 1 cut(s) 585
AccI GTMKAC 2 cut(s) 828, 919
AccII CGCG 1 cut(s) 1228
AciI CCGC 8 cut(s) 52, 55, 225, 275, 585, 664, 2165, 2201
AclWI GGATC 4 cut(s) 43, 1015, 2070, 2098
AcoI YGGCCR 3 cut(s) 74, 157, 2053
AcsI RAATTY 3 cut(s) 364, 478, 669
AcuI CTGAAG 3 cut(s) 402, 861, 1314
AcvI CACGTG 1 cut(s) 772
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 7 cut(s) 10, 139, 185, 500, 721, 1118, 1702
AfiI CCNNNNNNNGG 3 cut(s) 134, 908, 954
AjiI CACGTC 1 cut(s) 302
AjnI CCWGG 6 cut(s) 753, 1003, 1403, 1421, 1527, 1878
AjuI GAANNNNNNNTTGG 2 cut(s) 2004, 2036
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 5 cut(s) 522, 647, 752, 923, 1016
AlwI GGATC 4 cut(s) 43, 1015, 2070, 2098
AlwNI CAGNNNCTG 1 cut(s) 1025
AoxI GGCC 8 cut(s) 74, 92, 157, 751, 880, 1493, 2053, 2162
ApeKI GCWGC 3 cut(s) 1048, 1346, 1796
ApoI RAATTY 3 cut(s) 364, 478, 669
ArsI GACNNNNNNTTYG 4 cut(s) 1160, 1192, 1928, 1960
AseI ATTAAT 1 cut(s) 2129
AspLEI GCGC 3 cut(s) 265, 328, 1230
AspS9I GGNCC 5 cut(s) 92, 1168, 1283, 1401, 1989
AsuC2I CCSGG 2 cut(s) 78, 1909
AsuHPI GGTGA 8 cut(s) 112, 239, 619, 860, 1145, 1154, 1685, 1799
AvaII GGWCC 4 cut(s) 1168, 1283, 1401, 1989
BaeGI GKGCMC 2 cut(s) 1605, 2100
BaeI ACNNNNGTAYC 2 cut(s) 129, 162
BalI TGGCCA 1 cut(s) 2055
BanI GGYRCC 2 cut(s) 1602, 2185
BanII GRGCYC 1 cut(s) 105
BbrPI CACGTG 1 cut(s) 772
BbsI GAAGAC 3 cut(s) 171, 543, 1989
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 1035, 1333, 1783
BccI CCATC 5 cut(s) 1391, 1625, 1688, 1778, 2176
BceAI ACGGC 1 cut(s) 2199
BciT130I CCWGG 6 cut(s) 755, 1005, 1405, 1423, 1529, 1880
BciVI GTATCC 1 cut(s) 1341
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 2 cut(s) 78, 1909
BcoDI GTCTC 5 cut(s) 522, 647, 752, 923, 1016
BfaI CTAG 2 cut(s) 1968, 2193
BfmI CTRYAG 1 cut(s) 2106
BfuI GTATCC 1 cut(s) 1341
BisI GCNGC 6 cut(s) 53, 275, 1049, 1347, 1797, 2165
BlsI GCNGC 6 cut(s) 54, 276, 1050, 1348, 1798, 2166
BmcAI AGTACT 1 cut(s) 721
Bme1390I CCNGG 8 cut(s) 78, 755, 1005, 1405, 1423, 1529, 1880, 1909
Bme18I GGWCC 4 cut(s) 1168, 1283, 1401, 1989
BmgBI CACGTC 1 cut(s) 302
BmgT120I GGNCC 5 cut(s) 92, 1168, 1283, 1401, 1989
BmiI GGNNCC 7 cut(s) 93, 972, 1170, 1402, 1604, 1892, 2187
BmrFI CCNGG 8 cut(s) 78, 755, 1005, 1405, 1423, 1529, 1880, 1909
BmsI GCATC 7 cut(s) 4, 124, 212, 301, 1358, 2186, 2209
BoxI GACNNNNGTC 1 cut(s) 639
BpiI GAAGAC 3 cut(s) 171, 543, 1989
BplI GAGNNNNNCTC 4 cut(s) 89, 121, 1879, 1911
BpuEI CTTGAG 3 cut(s) 356, 548, 2168
BpuMI CCSGG 2 cut(s) 78, 1909
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 772
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 2 cut(s) 647, 752
BsaJI CCNNGG 6 cut(s) 77, 614, 754, 1003, 1404, 1422
BsaXI ACNNNNNCTCC 4 cut(s) 808, 838, 1849, 1879
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 908, 954
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 4 cut(s) 171, 1318, 1323, 1863
BseBI CCWGG 6 cut(s) 755, 1005, 1405, 1423, 1529, 1880
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 6 cut(s) 77, 614, 754, 1003, 1404, 1422
BseGI GGATG 7 cut(s) 433, 624, 1045, 1402, 1432, 1789, 2224
BseLI CCNNNNNNNGG 3 cut(s) 134, 908, 954
BseMII CTCAG 3 cut(s) 456, 572, 1032
BseNI ACTGG 4 cut(s) 171, 1318, 1323, 1863
BseRI GAGGAG 4 cut(s) 113, 829, 983, 1887
BseSI GKGCMC 2 cut(s) 1605, 2100
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 1035, 1333, 1783
BseYI CCCAGC 1 cut(s) 1434
Bsh1236I CGCG 1 cut(s) 1228
Bsh1285I CGRYCG 2 cut(s) 42, 160
BshFI GGCC 8 cut(s) 76, 94, 159, 753, 882, 1495, 2055, 2164
BshNI GGYRCC 2 cut(s) 1602, 2185
BshVI ATCGAT 1 cut(s) 38
BsiEI CGRYCG 2 cut(s) 42, 160
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 4 cut(s) 18, 77, 95, 1909
BslFI GGGAC 5 cut(s) 528, 1414, 1451, 1538, 1739
BslI CCNNNNNNNGG 3 cut(s) 134, 908, 954
BsmAI GTCTC 5 cut(s) 522, 647, 752, 923, 1016
BsmFI GGGAC 5 cut(s) 528, 1414, 1451, 1538, 1739
BsmI GAATGC 3 cut(s) 243, 1436, 2115
BsnI GGCC 8 cut(s) 76, 94, 159, 753, 882, 1495, 2055, 2164
Bso31I GGTCTC 2 cut(s) 647, 752
Bsp1286I GDGCHC 3 cut(s) 105, 1605, 2100
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
BspACI CCGC 8 cut(s) 52, 55, 225, 275, 585, 664, 2165, 2201
BspANI GGCC 8 cut(s) 76, 94, 159, 753, 882, 1495, 2055, 2164
BspCNI CTCAG 3 cut(s) 455, 573, 1031
BspDI ATCGAT 1 cut(s) 38
BspFNI CGCG 1 cut(s) 1228
BspHI TCATGA 2 cut(s) 387, 2234
BspLI GGNNCC 7 cut(s) 93, 972, 1170, 1402, 1604, 1892, 2187
BspPI GGATC 4 cut(s) 43, 1015, 2070, 2098
BspT107I GGYRCC 2 cut(s) 1602, 2185
BspTNI GGTCTC 2 cut(s) 647, 752
BsrBI CCGCTC 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 4 cut(s) 171, 1318, 1323, 1863
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 6 cut(s) 77, 614, 754, 1003, 1404, 1422
BssMI GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
BssNAI GTATAC 1 cut(s) 829
BssNI GRCGYC 1 cut(s) 168
Bst1107I GTATAC 1 cut(s) 829
Bst2UI CCWGG 6 cut(s) 755, 1005, 1405, 1423, 1529, 1880
Bst4CI ACNGT 4 cut(s) 404, 724, 1377, 2107
Bst6I CTCTTC 4 cut(s) 661, 688, 960, 1713
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 271
BstAUI TGTACA 1 cut(s) 498
BstBAI YACGTR 1 cut(s) 772
BstC8I GCNNGC 5 cut(s) 19, 629, 837, 1876, 2113
BstDEI CTNAG 6 cut(s) 442, 581, 1018, 1235, 1624, 1947
BstF5I GGATG 7 cut(s) 433, 624, 1045, 1402, 1432, 1789, 2224
BstFNI CGCG 1 cut(s) 1228
BstHHI GCGC 3 cut(s) 265, 328, 1230
BstKTI GATC 9 cut(s) 38, 42, 598, 1010, 1247, 1474, 2065, 2093, 2157
BstMAI GTCTC 5 cut(s) 522, 647, 752, 923, 1016
BstMBI GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
BstMCI CGRYCG 2 cut(s) 42, 160
BstMWI GCNNNNNNNGC 9 cut(s) 27, 100, 211, 260, 271, 377, 1187, 2183, 2198
BstNI CCWGG 6 cut(s) 755, 1005, 1405, 1423, 1529, 1880
BstNSI RCATGY 1 cut(s) 1150
BstPAI GACNNNNGTC 1 cut(s) 639
BstSCI CCNGG 8 cut(s) 76, 753, 1003, 1403, 1421, 1527, 1878, 1907
BstSFI CTRYAG 1 cut(s) 2106
BstSLI GKGCMC 2 cut(s) 1605, 2100
BstUI CGCG 1 cut(s) 1228
BstV1I GCAGC 3 cut(s) 1035, 1333, 1783
BstV2I GAAGAC 3 cut(s) 171, 543, 1989
BstX2I RGATCY 2 cut(s) 1007, 2090
BstYI RGATCY 2 cut(s) 1007, 2090
BstZ17I GTATAC 1 cut(s) 829
BstZI CGGCCG 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 38
BsuI GTATCC 1 cut(s) 1341
BsuRI GGCC 8 cut(s) 76, 94, 159, 753, 882, 1495, 2055, 2164
BsuTUI ATCGAT 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 1469
BtrI CACGTC 1 cut(s) 302
BtsCI GGATG 7 cut(s) 433, 624, 1045, 1402, 1432, 1789, 2224
BtsIMutI CAGTG 2 cut(s) 178, 1917
Cac8I GCNNGC 5 cut(s) 19, 629, 837, 1876, 2113
CaiI CAGNNNCTG 1 cut(s) 1025
CciI TCATGA 2 cut(s) 387, 2234
CfoI GCGC 3 cut(s) 265, 328, 1230
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 5 cut(s) 92, 1168, 1283, 1401, 1989
ClaI ATCGAT 1 cut(s) 38
CseI GACGC 2 cut(s) 19, 176
Csp6I GTAC 7 cut(s) 9, 138, 184, 499, 720, 1117, 1701
CviQI GTAC 7 cut(s) 9, 138, 184, 499, 720, 1117, 1701
DdeI CTNAG 6 cut(s) 442, 581, 1018, 1235, 1624, 1947
DpnI GATC 9 cut(s) 37, 41, 597, 1009, 1246, 1473, 2064, 2092, 2156
DpnII GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
DraI TTTAAA 1 cut(s) 690
DrdI GACNNNNNNGTC 1 cut(s) 1523
DseDI GACNNNNNNGTC 1 cut(s) 1523
EaeI YGGCCR 3 cut(s) 74, 157, 2053
EagI CGGCCG 1 cut(s) 157
Eam1104I CTCTTC 4 cut(s) 661, 688, 960, 1713
EarI CTCTTC 4 cut(s) 661, 688, 960, 1713
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 2 cut(s) 647, 752
Eco47I GGWCC 4 cut(s) 1168, 1283, 1401, 1989
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 3 cut(s) 402, 861, 1314
Eco72I CACGTG 1 cut(s) 772
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 3 cut(s) 1168, 1401, 1989
EcoRI GAATTC 1 cut(s) 478
EcoRII CCWGG 6 cut(s) 753, 1003, 1403, 1421, 1527, 1878
EcoT38I GRGCYC 1 cut(s) 105
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FaqI GGGAC 5 cut(s) 528, 1414, 1451, 1538, 1739
FauI CCCGC 1 cut(s) 578
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 2 cut(s) 828, 919
Fnu4HI GCNGC 6 cut(s) 53, 275, 1049, 1347, 1797, 2165
FokI GGATG 7 cut(s) 440, 631, 1052, 1409, 1439, 1796, 2231
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 6 cut(s) 53, 275, 1049, 1347, 1797, 2165
FspBI CTAG 2 cut(s) 1968, 2193
GlaI GCGC 3 cut(s) 264, 327, 1229
GluI GCNGC 6 cut(s) 53, 275, 1049, 1347, 1797, 2165
GsaI CCCAGC 1 cut(s) 1438
HaeIII GGCC 8 cut(s) 76, 94, 159, 753, 882, 1495, 2055, 2164
HapII CCGG 4 cut(s) 18, 77, 95, 1909
HgaI GACGC 2 cut(s) 19, 176
HhaI GCGC 3 cut(s) 265, 328, 1230
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 3 cut(s) 263, 326, 1228
HinP1I GCGC 3 cut(s) 263, 326, 1228
HindIII AAGCTT 3 cut(s) 149, 1102, 1957
HpaII CCGG 4 cut(s) 18, 77, 95, 1909
HphI GGTGA 8 cut(s) 112, 239, 619, 860, 1145, 1154, 1685, 1799
Hpy166II GTNNAC 6 cut(s) 407, 775, 829, 920, 960, 1516
Hpy188III TCNNGA 8 cut(s) 305, 388, 1166, 1341, 1885, 1933, 2088, 2235
Hpy8I GTNNAC 6 cut(s) 407, 775, 829, 920, 960, 1516
Hpy99I CGWCG 2 cut(s) 35, 306
HpyAV CCTTC 7 cut(s) 353, 418, 885, 1062, 1256, 1582, 1817
HpyCH4III ACNGT 4 cut(s) 404, 724, 1377, 2107
HpyCH4IV ACGT 2 cut(s) 301, 771
HpyCH4V TGCA 9 cut(s) 243, 299, 631, 804, 1181, 1349, 1730, 2115, 2222
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 100, 211, 260, 271, 377, 1187, 2183, 2198
HpyF3I CTNAG 6 cut(s) 442, 581, 1018, 1235, 1624, 1947
HpySE526I ACGT 2 cut(s) 301, 771
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 3 cut(s) 263, 326, 1228
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
LmnI GCTCC 5 cut(s) 100, 784, 877, 904, 970
Lsp1109I GCAGC 3 cut(s) 1035, 1333, 1783
LweI GCATC 7 cut(s) 4, 124, 212, 301, 1358, 2186, 2209
MaeI CTAG 2 cut(s) 1968, 2193
MaeII ACGT 2 cut(s) 301, 771
MaeIII GTNAC 7 cut(s) 607, 767, 1142, 1535, 2024, 2122, 2142
MalI GATC 9 cut(s) 37, 41, 597, 1009, 1246, 1473, 2064, 2092, 2156
MbiI CCGCTC 1 cut(s) 585
MboI GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 2 cut(s) 1007, 2090
MhlI GDGCHC 3 cut(s) 105, 1605, 2100
MlsI TGGCCA 1 cut(s) 2055
MluCI AATT 7 cut(s) 187, 364, 478, 485, 669, 1248, 1844
MluNI TGGCCA 1 cut(s) 2055
MlyI GAGTC 5 cut(s) 555, 836, 1366, 1761, 2090
MmeI TCCRAC 3 cut(s) 13, 1054, 1351
Mox20I TGGCCA 1 cut(s) 2055
MroNI GCCGGC 1 cut(s) 17
MscI TGGCCA 1 cut(s) 2055
MseI TTAA 6 cut(s) 210, 689, 1580, 1839, 1847, 2129
MslI CAYNNNNRTG 5 cut(s) 220, 525, 1140, 2049, 2120
Msp20I TGGCCA 1 cut(s) 2055
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 4 cut(s) 18, 77, 95, 1909
MspR9I CCNGG 8 cut(s) 78, 755, 1005, 1405, 1423, 1529, 1880, 1909
MunI CAATTG 1 cut(s) 1248
Mva1269I GAATGC 3 cut(s) 243, 1436, 2115
MvaI CCWGG 6 cut(s) 755, 1005, 1405, 1423, 1529, 1880
MvnI CGCG 1 cut(s) 1228
MwoI GCNNNNNNNGC 9 cut(s) 27, 100, 211, 260, 271, 377, 1187, 2183, 2198
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 2 cut(s) 78, 1909
NdeII GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
NgoMIV GCCGGC 1 cut(s) 17
NlaIV GGNNCC 7 cut(s) 93, 972, 1170, 1402, 1604, 1892, 2187
NmeAIII GCCGAG 3 cut(s) 135, 185, 729
NmuCI GTSAC 4 cut(s) 607, 767, 1142, 2122
NspI RCATGY 1 cut(s) 1150
PagI TCATGA 2 cut(s) 387, 2234
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 1756
PctI GAATGC 3 cut(s) 243, 1436, 2115
PdiI GCCGGC 1 cut(s) 19
PfeI GAWTC 8 cut(s) 257, 490, 851, 977, 992, 1013, 1127, 1667
PflFI GACNNNGTC 1 cut(s) 1993
PkrI GCNGC 6 cut(s) 54, 276, 1050, 1348, 1798, 2166
Ple19I CGATCG 1 cut(s) 42
PleI GAGTC 5 cut(s) 555, 836, 1365, 1760, 2089
PmaCI CACGTG 1 cut(s) 772
PmlI CACGTG 1 cut(s) 772
PpsI GAGTC 5 cut(s) 555, 836, 1365, 1760, 2089
Ppu21I YACGTR 1 cut(s) 772
PpuMI RGGWCCY 3 cut(s) 1168, 1401, 1989
PshAI GACNNNNGTC 1 cut(s) 639
PshBI ATTAAT 1 cut(s) 2129
PsiI TTATAA 1 cut(s) 1479
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 3 cut(s) 1168, 1401, 1989
Psp6I CCWGG 6 cut(s) 753, 1003, 1403, 1421, 1527, 1878
PspCI CACGTG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 1434
PspGI CCWGG 6 cut(s) 753, 1003, 1403, 1421, 1527, 1878
PspN4I GGNNCC 7 cut(s) 93, 972, 1170, 1402, 1604, 1892, 2187
PspPI GGNCC 5 cut(s) 92, 1168, 1283, 1401, 1989
PspPPI RGGWCCY 3 cut(s) 1168, 1401, 1989
PstNI CAGNNNCTG 1 cut(s) 1025
PsuI RGATCY 2 cut(s) 1007, 2090
PsyI GACNNNGTC 1 cut(s) 1993
PvuI CGATCG 1 cut(s) 42
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 7 cut(s) 10, 139, 185, 500, 721, 1118, 1702
RsaNI GTAC 7 cut(s) 9, 138, 184, 499, 720, 1117, 1701
RseI CAYNNNNRTG 5 cut(s) 220, 525, 1140, 2049, 2120
SacI GAGCTC 1 cut(s) 105
SaqAI TTAA 6 cut(s) 210, 689, 1580, 1839, 1847, 2129
SatI GCNGC 6 cut(s) 53, 275, 1049, 1347, 1797, 2165
Sau3AI GATC 9 cut(s) 35, 39, 595, 1007, 1244, 1471, 2062, 2090, 2154
Sau96I GGNCC 5 cut(s) 92, 1168, 1283, 1401, 1989
ScaI AGTACT 1 cut(s) 721
SchI GAGTC 5 cut(s) 555, 836, 1366, 1761, 2090
ScrFI CCNGG 8 cut(s) 78, 755, 1005, 1405, 1423, 1529, 1880, 1909
SduI GDGCHC 3 cut(s) 105, 1605, 2100
SfaNI GCATC 7 cut(s) 4, 124, 212, 301, 1358, 2186, 2209
SfcI CTRYAG 1 cut(s) 2106
SinI GGWCC 4 cut(s) 1168, 1283, 1401, 1989
SmiMI CAYNNNNRTG 5 cut(s) 220, 525, 1140, 2049, 2120
SmlI CTYRAG 3 cut(s) 335, 563, 2147
SmoI CTYRAG 3 cut(s) 335, 563, 2147
Sse9I AATT 7 cut(s) 187, 364, 478, 485, 669, 1248, 1844
SsiI CCGC 8 cut(s) 52, 55, 225, 275, 585, 664, 2165, 2201
SspI AATATT 2 cut(s) 464, 2133
SspMI CTAG 2 cut(s) 1968, 2193
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 8 cut(s) 76, 753, 1003, 1403, 1421, 1527, 1878, 1907
TaaI ACNGT 4 cut(s) 404, 724, 1377, 2107
TaiI ACGT 2 cut(s) 304, 774
TaqI TCGA 6 cut(s) 38, 220, 1360, 1686, 1750, 1934
TasI AATT 7 cut(s) 187, 364, 478, 485, 669, 1248, 1844
TatI WGTACW 3 cut(s) 8, 498, 719
TauI GCSGC 3 cut(s) 55, 277, 2167
TfiI GAWTC 8 cut(s) 257, 490, 851, 977, 992, 1013, 1127, 1667
Tru1I TTAA 6 cut(s) 210, 689, 1580, 1839, 1847, 2129
Tru9I TTAA 6 cut(s) 210, 689, 1580, 1839, 1847, 2129
TscAI CASTG 2 cut(s) 178, 1924
TseFI GTSAC 4 cut(s) 607, 767, 1142, 2122
TseI GCWGC 3 cut(s) 1048, 1346, 1796
Tsp45I GTSAC 4 cut(s) 607, 767, 1142, 2122
TspGWI ACGGA 1 cut(s) 792
TspRI CASTG 2 cut(s) 178, 1924
Tth111I GACNNNGTC 1 cut(s) 1993
VpaK11BI GGWCC 4 cut(s) 1168, 1283, 1401, 1989
VspI ATTAAT 1 cut(s) 2129
XapI RAATTY 3 cut(s) 364, 478, 669
XceI RCATGY 1 cut(s) 1150
XmiI GTMKAC 2 cut(s) 828, 919
XspI CTAG 2 cut(s) 1968, 2193
ZrmI AGTACT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.