RchiOBHm_Chr1g0383861

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006536
Physical Location & Seq
Reverse (-)
67999915 .. 68004475
4561 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60674

Sequence Viewer

Length: 2229 bp
ATGGAGAGTACAGATTCCGGCGATTTCAGCGTCGGATCGATCGTGTGGGTACGGCGAAGGAACGGTTCGTGGTGGCCGGGGAAGATAGTCGGACCGGAAGAGCTCTCCACTTCGCATCTAACCTCGCCTCGCTCTGGTACCCCCGTCAAGCTTCTCGGTAGAGAAGACGCCAGTGTTGATTGGTACAATCTGGAAAAGTCCAAGCGTGTTAAACCATTTCGATGCGGTGATTTTGATGATTGCATTCACAAGGCTGAATCGGCACAAGGTATGCCGCCAAAGAAAAGAGAAAAATATGCACGTCGAGAAGATGCTATTCTTCATGCGCTTGAACTTGAGAGGCAACTGTTGAGGAAACAAGGAAAGTCAACTTCTGAATACGTGAACAGTAAATATGGTGCTGTGAAGGAGGCTGTTATACCCTCAGAAAGTTTGGGAAAAGACCATTCAAAACTTGGGAATTCCAAATCGCATCAGTTATACAAGCGAAAAGATTCGTTTCATAAGAAGGATATTATAGGTAGTCCCCTGCCTTCTCAAAGACTCAAAGAGGGAACCCAATTGAGTGGTGAAGATGATCGTTTTGAAGTGACACCTCGAATGAGAGGCTTGCAGGACTTTGGTCTCAAAATTGCCTCTTCAAAGCGAAAACTTTCATCTTCCTCTACTTCAAATGGTTTTTGGAAACCCACAATTGATGGTACTTATCAAGCATTTGCTCATGGTGGCCTCAGCCTGGGAGGAACAAGTCACGTGAACGGAGTGGAGCAGACGGGAGCTATTTGCAGAGCAAAGAGGAGTAAATGTGTATACTTGCCAGCTGACTCTGGTGATTCTTTGGAGTATGAAGCAGCACCTGCAGGCCATATTGAGATATCAGCTTCCCCCATTGGCACTCGTCTACATGCTGAGAGTTTGATTGAGGACAACACTTCTGGGTTTACAGAAGATCTAGAGGAGTCCAATTTTTCTGAAACTGATTCCTCTGACCATGGATCTGATTCCTCAGAGACTGAGCCGGATATGGATGAAGAGATGCCTTCATTTTCAGATCCAGAAGTAGGAAGATATGAAGCACAAGAACGTAGAAGCTTGATTGATGAGGAACCAGATGAATCAACACATTCTGGCGACATGCCTCACCTCTATAATCAGGACCCTTATTTTGCAAGTGAAGCTGTGTCCAAATGGCAGTTGAAAGGGAAAAGGAATACCCGCAATCTTAGTAAAAGATCAATTGATACAACTGAAGGAAGAGGTTATTTTTATGGACCATATTCTGAAGAAAAGGCTGAGATGAGTGGAATGGACGATGGGTACCCCCTATCAAGAGCTGCATCAAGAAGTCGAAACAGGGTTGGTCTGAATATGATTGATTGGGAGGATGGGACCTGGGAAGACAGGTCTGCCTGGGATGAATGCTGGGACATCAAAAGAGAGCGATTTGACCCGGTATATGATGATCGCTATAATCATCGCAGGAGGCCAAAATACTTGATAGATGTGGACTTGAAAGTCCAGGCAAGTTACCAGAAAGAGCCTGTCCCTATTGTTTCTCTGATGAGCAAGTTAAACGGGAAGGCAATAATAGGGCACCCCATCCAAGTTGAAGCCTTAGAAGATGGTTTATCTAACACGCTTCTTTCAACATTTGATGATTCTGGTGAGGAAGCAATTGACAATGATGGGGGTACAACTCTTCCCCACGCATGGAGGACTGCAAGGAGGACAGCAAATGTTAGAGTCCCACGTCCTCATTTATTTTCTACATTGGATGGTGATGAGGCTGCCGATGACCTTCCCTTTTTCGATGAAGGTAGACCACCATTCAAGAAATTAAATATGGGGAGTTCCAGTCACAAGGCAAGCCAGGTCAGGAGGAACCCTCCTCACAACTCCCGGCTTCTCACTGATAGAAAGTTCTCGAAGAAGGTGTCTAAGAAAGTAAGCTTATCATCTAGCCTGAAAACGAAAACGAGGACCTTATCTTCTTTAGCCATTCAACAGAACTATGGTAACAAGGGAATGATACAATATGATAGTGGCGGCCAAAGGGATAGGTTGGTGAAACCAGAGTCTTCTGTATCTGTGCCCACAACTGTAGCTTGCATTCCTGTGAAATTAGTTTTCAGTAGGTTACTCGAGAAGATCAATAGGCCGCCATCAAAAGCAACAGCAGCAGCAGCCAGTAATGCGGTCTTATTGAATGGGGATGTGGAGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

742

Amino Acids

82.75

Weight (kDa)

6.71

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 12 - 92 8.3e-11 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 865
AasI GACNNNNNNGTC 1 cut(s) 1514
Acc36I ACCTGC 1 cut(s) 865
Acc65I GGTACC 2 cut(s) 137, 1317
AccB1I GGYRCC 3 cut(s) 137, 1317, 1593
AccI GTMKAC 3 cut(s) 810, 901, 1819
AciI CCGC 6 cut(s) 225, 275, 1216, 2046, 2159, 2195
AclWI GGATC 3 cut(s) 43, 1003, 1046
AcoI YGGCCR 2 cut(s) 74, 2047
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 2 cut(s) 1269, 1302
AcvI CACGTG 1 cut(s) 754
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 7 cut(s) 10, 51, 139, 185, 703, 1319, 1693
AfiI CCNNNNNNNGG 4 cut(s) 134, 736, 1061, 1710
AjiI CACGTC 2 cut(s) 302, 1751
AjnI CCWGG 5 cut(s) 735, 1391, 1409, 1518, 1869
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 2 cut(s) 629, 1004
AlwI GGATC 3 cut(s) 43, 1003, 1046
AlwNI CAGNNNCTG 2 cut(s) 857, 1013
Ama87I CYCGRG 1 cut(s) 2141
AoxI GGCC 6 cut(s) 74, 727, 862, 1484, 2047, 2156
ApeKI GCWGC 6 cut(s) 851, 1334, 1787, 2177, 2180, 2183
ApoI RAATTY 1 cut(s) 460
ArsI GACNNNNNNTTYG 4 cut(s) 1148, 1180, 1919, 1951
Asp700I GAANNNNTTC 2 cut(s) 493, 652
Asp718I GGTACC 2 cut(s) 137, 1317
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 5 cut(s) 92, 1156, 1271, 1389, 1980
AsuC2I CCSGG 3 cut(s) 78, 1451, 1900
AsuHPI GGTGA 7 cut(s) 239, 581, 842, 1133, 1676, 1790, 2077
AvaI CYCGRG 1 cut(s) 2141
AvaII GGWCC 5 cut(s) 92, 1156, 1271, 1389, 1980
BaeGI GKGCMC 2 cut(s) 1596, 2094
BaeI ACNNNNGTAYC 2 cut(s) 129, 162
BanI GGYRCC 3 cut(s) 137, 1317, 1593
BanII GRGCYC 1 cut(s) 105
BbrPI CACGTG 1 cut(s) 754
BbsI GAAGAC 3 cut(s) 171, 1404, 2070
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 731
BbvI GCAGC 6 cut(s) 863, 1321, 1774, 2189, 2192, 2195
BccI CCATC 8 cut(s) 692, 1307, 1379, 1607, 1616, 1679, 1769, 2170
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 5 cut(s) 737, 1393, 1411, 1520, 1871
BcnI CCSGG 3 cut(s) 78, 1451, 1900
BcoDI GTCTC 2 cut(s) 629, 1004
BfaI CTAG 2 cut(s) 953, 1959
BfmI CTRYAG 2 cut(s) 858, 2100
BfuAI ACCTGC 1 cut(s) 865
BglII AGATCT 1 cut(s) 949
BisI GCNGC 9 cut(s) 275, 852, 1335, 1788, 2047, 2159, 2178, 2181, 2184
BlsI GCNGC 9 cut(s) 276, 853, 1336, 1789, 2048, 2160, 2179, 2182, 2185
Bme1390I CCNGG 8 cut(s) 78, 737, 1393, 1411, 1451, 1520, 1871, 1900
Bme18I GGWCC 5 cut(s) 92, 1156, 1271, 1389, 1980
BmeT110I CYCGRG 1 cut(s) 2141
BmgBI CACGTC 2 cut(s) 302, 1751
BmgT120I GGNCC 5 cut(s) 92, 1156, 1271, 1389, 1980
BmiI GGNNCC 8 cut(s) 139, 556, 1107, 1158, 1319, 1390, 1595, 1883
BmrFI CCNGG 8 cut(s) 78, 737, 1393, 1411, 1451, 1520, 1871, 1900
BmsI GCATC 6 cut(s) 124, 212, 301, 481, 1026, 1346
BoxI GACNNNNGTC 1 cut(s) 621
BpiI GAAGAC 3 cut(s) 171, 1404, 2070
BplI GAGNNNNNCTC 2 cut(s) 1870, 1902
Bpu10I CCTNAGC 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 3 cut(s) 78, 1451, 1900
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 2 cut(s) 382, 754
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 5 cut(s) 77, 736, 991, 1392, 1410
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 790, 820, 1840, 1870
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 736, 1061, 1710
Bse1I ACTGG 3 cut(s) 171, 1854, 2187
BseBI CCWGG 5 cut(s) 737, 1393, 1411, 1520, 1871
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 5 cut(s) 77, 736, 991, 1392, 1410
BseGI GGATG 6 cut(s) 1033, 1390, 1420, 1599, 1780, 2218
BseLI CCNNNNNNNGG 4 cut(s) 134, 736, 1061, 1710
BseMII CTCAG 6 cut(s) 438, 745, 900, 1005, 1020, 1284
BseNI ACTGG 3 cut(s) 171, 1854, 2187
BseRI GAGGAG 3 cut(s) 811, 971, 1878
BseSI GKGCMC 2 cut(s) 1596, 2094
BseXI GCAGC 6 cut(s) 863, 1321, 1774, 2189, 2192, 2195
BseYI CCCAGC 1 cut(s) 1422
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 6 cut(s) 76, 729, 864, 1486, 2049, 2158
BshNI GGYRCC 3 cut(s) 137, 1317, 1593
BshVI ATCGAT 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 2141
BsiSI CCGG 6 cut(s) 18, 77, 95, 1019, 1451, 1900
BslFI GGGAC 5 cut(s) 510, 1402, 1439, 1529, 1730
BslI CCNNNNNNNGG 4 cut(s) 134, 736, 1061, 1710
BsmAI GTCTC 2 cut(s) 629, 1004
BsmFI GGGAC 5 cut(s) 510, 1402, 1439, 1529, 1730
BsmI GAATGC 3 cut(s) 243, 1424, 2109
BsnI GGCC 6 cut(s) 76, 729, 864, 1486, 2049, 2158
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 2141
Bsp1286I GDGCHC 3 cut(s) 105, 1596, 2094
Bsp143I GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
Bsp19I CCATGG 1 cut(s) 991
BspACI CCGC 6 cut(s) 225, 275, 1216, 2046, 2159, 2195
BspANI GGCC 6 cut(s) 76, 729, 864, 1486, 2049, 2158
BspCNI CTCAG 6 cut(s) 437, 744, 901, 1006, 1019, 1285
BspDI ATCGAT 1 cut(s) 38
BspLI GGNNCC 8 cut(s) 139, 556, 1107, 1158, 1319, 1390, 1595, 1883
BspMAI CTGCAG 1 cut(s) 862
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 3 cut(s) 43, 1003, 1046
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 3 cut(s) 137, 1317, 1593
BspTNI GGTCTC 1 cut(s) 629
BsrI ACTGG 3 cut(s) 171, 1854, 2187
BssECI CCNNGG 5 cut(s) 77, 736, 991, 1392, 1410
BssMI GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
BssNAI GTATAC 1 cut(s) 811
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 991
Bst1107I GTATAC 1 cut(s) 811
Bst2UI CCWGG 5 cut(s) 737, 1393, 1411, 1520, 1871
Bst4CI ACNGT 4 cut(s) 65, 348, 389, 2101
Bst6I CTCTTC 5 cut(s) 93, 643, 1026, 1249, 1704
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 2 cut(s) 382, 754
BstC8I GCNNGC 5 cut(s) 611, 819, 862, 1867, 2107
BstDEI CTNAG 9 cut(s) 424, 731, 909, 1006, 1014, 1223, 1293, 1615, 1938
BstDSI CCRYGG 1 cut(s) 991
BstF5I GGATG 6 cut(s) 1033, 1390, 1420, 1599, 1780, 2218
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 9 cut(s) 38, 42, 580, 952, 998, 1054, 1235, 1465, 2151
BstMAI GTCTC 2 cut(s) 629, 1004
BstMBI GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 7 cut(s) 27, 260, 857, 1175, 2177, 2183, 2192
BstNI CCWGG 5 cut(s) 737, 1393, 1411, 1520, 1871
BstNSI RCATGY 2 cut(s) 908, 1138
BstPAI GACNNNNGTC 1 cut(s) 621
BstSCI CCNGG 8 cut(s) 76, 735, 1391, 1409, 1449, 1518, 1869, 1898
BstSFI CTRYAG 2 cut(s) 858, 2100
BstSLI GKGCMC 2 cut(s) 1596, 2094
BstV1I GCAGC 6 cut(s) 863, 1321, 1774, 2189, 2192, 2195
BstV2I GAAGAC 3 cut(s) 171, 1404, 2070
BstX2I RGATCY 3 cut(s) 949, 995, 1051
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 3 cut(s) 949, 995, 1051
BstZ17I GTATAC 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 6 cut(s) 76, 729, 864, 1486, 2049, 2158
BsuTUI ATCGAT 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 991
BtgZI GCGATG 1 cut(s) 1460
BtrI CACGTC 2 cut(s) 302, 1751
BtsCI GGATG 6 cut(s) 1033, 1390, 1420, 1599, 1780, 2218
BtsIMutI CAGTG 2 cut(s) 178, 1908
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 5 cut(s) 611, 819, 862, 1867, 2107
CaiI CAGNNNCTG 2 cut(s) 857, 1013
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 5 cut(s) 92, 1156, 1271, 1389, 1980
ClaI ATCGAT 1 cut(s) 38
CpoI CGGWCCG 1 cut(s) 92
CseI GACGC 2 cut(s) 19, 176
Csp6I GTAC 7 cut(s) 9, 50, 138, 184, 702, 1318, 1692
CspI CGGWCCG 1 cut(s) 92
CviAII CATG 6 cut(s) 323, 722, 905, 992, 1135, 1710
CviQI GTAC 7 cut(s) 9, 50, 138, 184, 702, 1318, 1692
DdeI CTNAG 9 cut(s) 424, 731, 909, 1006, 1014, 1223, 1293, 1615, 1938
DpnI GATC 9 cut(s) 37, 41, 579, 951, 997, 1053, 1234, 1464, 2150
DpnII GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
DrdI GACNNNNNNGTC 1 cut(s) 1514
DseDI GACNNNNNNGTC 1 cut(s) 1514
EaeI YGGCCR 2 cut(s) 74, 2047
Eam1104I CTCTTC 5 cut(s) 93, 643, 1026, 1249, 1704
EarI CTCTTC 5 cut(s) 93, 643, 1026, 1249, 1704
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 876
Eco47I GGWCC 5 cut(s) 92, 1156, 1271, 1389, 1980
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 2 cut(s) 1269, 1302
Eco72I CACGTG 1 cut(s) 754
Eco88I CYCGRG 1 cut(s) 2141
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 3 cut(s) 1156, 1389, 1980
EcoRI GAATTC 1 cut(s) 460
EcoRII CCWGG 5 cut(s) 735, 1391, 1409, 1518, 1869
EcoRV GATATC 1 cut(s) 876
EcoT14I CCWWGG 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 991
FaeI CATG 6 cut(s) 326, 725, 908, 995, 1138, 1713
FaqI GGGAC 5 cut(s) 510, 1402, 1439, 1529, 1730
FatI CATG 6 cut(s) 322, 721, 904, 991, 1134, 1709
FauI CCCGC 1 cut(s) 1223
FblI GTMKAC 3 cut(s) 810, 901, 1819
Fnu4HI GCNGC 9 cut(s) 275, 852, 1335, 1788, 2047, 2159, 2178, 2181, 2184
FokI GGATG 6 cut(s) 1040, 1397, 1427, 1586, 1787, 2225
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 9 cut(s) 275, 852, 1335, 1788, 2047, 2159, 2178, 2181, 2184
FspBI CTAG 2 cut(s) 953, 1959
GlaI GCGC 1 cut(s) 327
GluI GCNGC 9 cut(s) 275, 852, 1335, 1788, 2047, 2159, 2178, 2181, 2184
GsaI CCCAGC 1 cut(s) 1426
HaeIII GGCC 6 cut(s) 76, 729, 864, 1486, 2049, 2158
HapII CCGG 6 cut(s) 18, 77, 95, 1019, 1451, 1900
HgaI GACGC 2 cut(s) 19, 176
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 6 cut(s) 326, 725, 908, 995, 1138, 1713
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 3 cut(s) 149, 1090, 1948
HpaII CCGG 6 cut(s) 18, 77, 95, 1019, 1451, 1900
HphI GGTGA 7 cut(s) 239, 581, 842, 1133, 1676, 1790, 2077
Hpy166II GTNNAC 8 cut(s) 369, 385, 757, 811, 902, 942, 1507, 1820
Hpy8I GTNNAC 8 cut(s) 369, 385, 757, 811, 902, 942, 1507, 1820
Hpy99I CGWCG 2 cut(s) 35, 306
HpyCH4III ACNGT 4 cut(s) 65, 348, 389, 2101
HpyCH4IV ACGT 5 cut(s) 301, 381, 753, 1084, 1750
HpyCH4V TGCA 9 cut(s) 243, 299, 613, 786, 860, 1169, 1337, 1721, 2109
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 260, 857, 1175, 2177, 2183, 2192
HpyF3I CTNAG 9 cut(s) 424, 731, 909, 1006, 1014, 1223, 1293, 1615, 1938
HpySE526I ACGT 5 cut(s) 301, 381, 753, 1084, 1750
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 6 cut(s) 326, 725, 908, 995, 1138, 1713
HspAI GCGC 1 cut(s) 326
KpnI GGTACC 2 cut(s) 141, 1321
Kzo9I GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 766, 776
Lsp1109I GCAGC 6 cut(s) 863, 1321, 1774, 2189, 2192, 2195
LweI GCATC 6 cut(s) 124, 212, 301, 481, 1026, 1346
MaeI CTAG 2 cut(s) 953, 1959
MaeII ACGT 5 cut(s) 301, 381, 753, 1084, 1750
MaeIII GTNAC 6 cut(s) 589, 749, 1526, 1856, 2015, 2136
MalI GATC 9 cut(s) 37, 41, 579, 951, 997, 1053, 1234, 1464, 2150
MboI GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
MfeI CAATTG 4 cut(s) 560, 693, 1236, 1674
MflI RGATCY 3 cut(s) 949, 995, 1051
MhlI GDGCHC 3 cut(s) 105, 1596, 2094
MluCI AATT 9 cut(s) 460, 560, 630, 693, 964, 1236, 1674, 1835, 2120
MlyI GAGTC 5 cut(s) 537, 818, 968, 1752, 2084
MmeI TCCRAC 2 cut(s) 13, 70
MroXI GAANNNNTTC 2 cut(s) 493, 652
MseI TTAA 3 cut(s) 210, 1571, 1838
MslI CAYNNNNRTG 2 cut(s) 220, 2114
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 6 cut(s) 18, 77, 95, 1019, 1451, 1900
MspR9I CCNGG 8 cut(s) 78, 737, 1393, 1411, 1451, 1520, 1871, 1900
MunI CAATTG 4 cut(s) 560, 693, 1236, 1674
Mva1269I GAATGC 3 cut(s) 243, 1424, 2109
MvaI CCWGG 5 cut(s) 737, 1393, 1411, 1520, 1871
MwoI GCNNNNNNNGC 7 cut(s) 27, 260, 857, 1175, 2177, 2183, 2192
NciI CCSGG 3 cut(s) 78, 1451, 1900
NcoI CCATGG 1 cut(s) 991
NdeII GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
NlaIII CATG 6 cut(s) 326, 725, 908, 995, 1138, 1713
NlaIV GGNNCC 8 cut(s) 139, 556, 1107, 1158, 1319, 1390, 1595, 1883
NmuCI GTSAC 3 cut(s) 589, 749, 1856
NspI RCATGY 2 cut(s) 908, 1138
PaeR7I CTCGAG 1 cut(s) 2141
PaqCI CACCTGC 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 3 cut(s) 243, 1424, 2109
PdmI GAANNNNTTC 2 cut(s) 493, 652
PfeI GAWTC 8 cut(s) 14, 257, 494, 833, 980, 1001, 1115, 1658
PkrI GCNGC 9 cut(s) 276, 853, 1336, 1789, 2048, 2160, 2179, 2182, 2185
Ple19I CGATCG 1 cut(s) 42
PleI GAGTC 5 cut(s) 537, 818, 967, 1751, 2083
PmaCI CACGTG 1 cut(s) 754
PmlI CACGTG 1 cut(s) 754
PpsI GAGTC 5 cut(s) 537, 818, 967, 1751, 2083
Ppu21I YACGTR 2 cut(s) 382, 754
PpuMI RGGWCCY 3 cut(s) 1156, 1389, 1980
PshAI GACNNNNGTC 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 3 cut(s) 1156, 1389, 1980
Psp6I CCWGG 5 cut(s) 735, 1391, 1409, 1518, 1869
PspCI CACGTG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 1422
PspGI CCWGG 5 cut(s) 735, 1391, 1409, 1518, 1869
PspN4I GGNNCC 8 cut(s) 139, 556, 1107, 1158, 1319, 1390, 1595, 1883
PspPI GGNCC 5 cut(s) 92, 1156, 1271, 1389, 1980
PspPPI RGGWCCY 3 cut(s) 1156, 1389, 1980
PstI CTGCAG 1 cut(s) 862
PstNI CAGNNNCTG 2 cut(s) 857, 1013
PsuI RGATCY 3 cut(s) 949, 995, 1051
PvuI CGATCG 1 cut(s) 42
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 7 cut(s) 10, 51, 139, 185, 703, 1319, 1693
RsaNI GTAC 7 cut(s) 9, 50, 138, 184, 702, 1318, 1692
RseI CAYNNNNRTG 2 cut(s) 220, 2114
Rsr2I CGGWCCG 1 cut(s) 92
RsrII CGGWCCG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 3 cut(s) 210, 1571, 1838
SatI GCNGC 9 cut(s) 275, 852, 1335, 1788, 2047, 2159, 2178, 2181, 2184
Sau3AI GATC 9 cut(s) 35, 39, 577, 949, 995, 1051, 1232, 1462, 2148
Sau96I GGNCC 5 cut(s) 92, 1156, 1271, 1389, 1980
SbfI CCTGCAGG 1 cut(s) 862
SchI GAGTC 5 cut(s) 537, 818, 968, 1752, 2084
ScrFI CCNGG 8 cut(s) 78, 737, 1393, 1411, 1451, 1520, 1871, 1900
SdaI CCTGCAGG 1 cut(s) 862
SduI GDGCHC 3 cut(s) 105, 1596, 2094
SfaNI GCATC 6 cut(s) 124, 212, 301, 481, 1026, 1346
SfcI CTRYAG 2 cut(s) 858, 2100
Sfr274I CTCGAG 1 cut(s) 2141
SinI GGWCC 5 cut(s) 92, 1156, 1271, 1389, 1980
SlaI CTCGAG 1 cut(s) 2141
SmiMI CAYNNNNRTG 2 cut(s) 220, 2114
SmlI CTYRAG 2 cut(s) 335, 2141
SmoI CTYRAG 2 cut(s) 335, 2141
Sse8387I CCTGCAGG 1 cut(s) 862
Sse9I AATT 9 cut(s) 460, 560, 630, 693, 964, 1236, 1674, 1835, 2120
SsiI CCGC 6 cut(s) 225, 275, 1216, 2046, 2159, 2195
SspMI CTAG 2 cut(s) 953, 1959
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 8 cut(s) 76, 735, 1391, 1409, 1449, 1518, 1869, 1898
StyI CCWWGG 1 cut(s) 991
TaaI ACNGT 4 cut(s) 65, 348, 389, 2101
TaiI ACGT 5 cut(s) 304, 384, 756, 1087, 1753
TaqI TCGA 8 cut(s) 38, 220, 304, 598, 1348, 1809, 1925, 2142
TasI AATT 9 cut(s) 460, 560, 630, 693, 964, 1236, 1674, 1835, 2120
TatI WGTACW 1 cut(s) 8
TauI GCSGC 3 cut(s) 277, 2049, 2161
TfiI GAWTC 8 cut(s) 14, 257, 494, 833, 980, 1001, 1115, 1658
Tru1I TTAA 3 cut(s) 210, 1571, 1838
Tru9I TTAA 3 cut(s) 210, 1571, 1838
TscAI CASTG 2 cut(s) 178, 1915
TseFI GTSAC 3 cut(s) 589, 749, 1856
TseI GCWGC 6 cut(s) 851, 1334, 1787, 2177, 2180, 2183
Tsp45I GTSAC 3 cut(s) 589, 749, 1856
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 2 cut(s) 178, 1915
VpaK11BI GGWCC 5 cut(s) 92, 1156, 1271, 1389, 1980
XapI RAATTY 1 cut(s) 460
XbaI TCTAGA 1 cut(s) 952
XceI RCATGY 2 cut(s) 908, 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 2141
XmiI GTMKAC 3 cut(s) 810, 901, 1819
XmnI GAANNNNTTC 2 cut(s) 493, 652
XspI CTAG 2 cut(s) 953, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.