Rmu_sc0005139.1_g000032

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005139.1
OG0006536
Physical Location & Seq
Forward (+)
164324 .. 165628
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005139.1_g000032.1.cds

Sequence Viewer

Length: 1206 bp
atggaaaccctagaagccctgacccgaatttctccaattggatcctccatattcgtctctattcggatctctcttgatgaggaaccagatgaatcaacacattctggcgacatgcctcacctctataatcaggacccttattttgcaagtgaagctgtgtccaaatggcagttgaaagggaaaaggaatacccgcaatcttagtaaaagatcaattgatacaactgaaggaagaggttatttttatggaccatattctgaagaaaaggctgagatgagtggaatggacgatgggtaccccctatcaagagctgcatcaagaagtcgaaacagggttggtctgaatatgattgattgggaggatgggacctgggaagacaggtctgcctgggatgaatgctgggacatcaaaagagagcgatttgacccggtatatgatgatcgctataatcatcgcaggaggccaaaatacttgatagatgtggacttgaaagtccaggcaagttaccagaaagagcctgtccctattgtttctctgatgagcaagttaaacgggaaggcaataatagggcaccccatccaagttgaagccttagaagatggtttatctaacacgcttctttcaacatttgatgattctggtgaggaagcaattgacaatgatgggggtacaactcttccccacgcatggaggactgcaaggaggacagcaaatgttagagtcccacgtcctcatttattttctacattggatggtgatgaggctgccgatgaccttccctttttcgatgaaggtagaccaccattcaagaaattaaatttggggagttccagtcacaaggcaagccaggtcaggaggaaccctcctcacaactcccggcttctcactgatagaaagttctcgaagaaggtgtctaagaaagtaagcttatcatctagcctgaaaacgaaaacgaggaccttatcttctttagccattctacagaactatggtaacaagggaatgacacaatatgatagtggcggccaaagggataggttggtgaaaccagagtcttctgtatctgtgcccacaactgtagcttgcattcctgtgaaattagttttcagtaggttactcgagaagatcaataggccgccatcaaaagcaacagcagcagcagccagtaatgcggtcttattgaacggggatgtggagagaagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

401

Amino Acids

44.98

Weight (kDa)

8.8

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc65I GGTACC 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 294, 570
AccI GTMKAC 1 cut(s) 796
AciI CCGC 4 cut(s) 193, 1023, 1136, 1172
AclWI GGATC 3 cut(s) 36, 49, 74
AcoI YGGCCR 1 cut(s) 1024
AcsI RAATTY 2 cut(s) 27, 817
AcuI CTGAAG 2 cut(s) 246, 279
AfaI GTAC 2 cut(s) 296, 670
AfiI CCNNNNNNNGG 1 cut(s) 687
AgsI TTSAA 6 cut(s) 175, 490, 587, 624, 808, 1183
AjiI CACGTC 1 cut(s) 728
AjnI CCWGG 4 cut(s) 368, 386, 495, 846
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
AluBI AGCT 4 cut(s) 155, 311, 927, 1082
AluI AGCT 4 cut(s) 155, 311, 927, 1082
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 3 cut(s) 36, 49, 74
Ama87I CYCGRG 1 cut(s) 1118
AoxI GGCC 3 cut(s) 461, 1024, 1133
ApeKI GCWGC 5 cut(s) 311, 764, 1154, 1157, 1160
ApoI RAATTY 2 cut(s) 27, 817
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 896, 928
Asp718I GGTACC 1 cut(s) 294
AspS9I GGNCC 4 cut(s) 133, 248, 366, 957
AsuC2I CCSGG 2 cut(s) 428, 877
AsuHPI GGTGA 4 cut(s) 110, 653, 767, 1054
AvaI CYCGRG 1 cut(s) 1118
AvaII GGWCC 4 cut(s) 133, 248, 366, 957
BaeGI GKGCMC 2 cut(s) 573, 1071
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 2 cut(s) 294, 570
BbsI GAAGAC 2 cut(s) 381, 1047
BbvI GCAGC 5 cut(s) 298, 751, 1166, 1169, 1172
BccI CCATC 7 cut(s) 284, 356, 584, 593, 656, 746, 1147
BciT130I CCWGG 4 cut(s) 370, 388, 497, 848
BcnI CCSGG 2 cut(s) 428, 877
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 11, 936
BfmI CTRYAG 2 cut(s) 980, 1077
BisI GCNGC 7 cut(s) 312, 765, 1024, 1136, 1155, 1158, 1161
BlsI GCNGC 7 cut(s) 313, 766, 1025, 1137, 1156, 1159, 1162
Bme1390I CCNGG 6 cut(s) 370, 388, 428, 497, 848, 877
Bme18I GGWCC 4 cut(s) 133, 248, 366, 957
BmeT110I CYCGRG 1 cut(s) 1118
BmgBI CACGTC 1 cut(s) 728
BmgT120I GGNCC 4 cut(s) 133, 248, 366, 957
BmiI GGNNCC 7 cut(s) 43, 84, 135, 296, 367, 572, 860
BmrFI CCNGG 6 cut(s) 370, 388, 428, 497, 848, 877
BmsI GCATC 1 cut(s) 323
BpiI GAAGAC 2 cut(s) 381, 1047
BplI GAGNNNNNCTC 2 cut(s) 847, 879
BpuMI CCSGG 2 cut(s) 428, 877
BsaJI CCNNGG 2 cut(s) 369, 387
BsaXI ACNNNNNCTCC 2 cut(s) 817, 847
Bsc4I CCNNNNNNNGG 1 cut(s) 687
Bse1I ACTGG 2 cut(s) 831, 1164
BseBI CCWGG 4 cut(s) 370, 388, 497, 848
BseDI CCNNGG 2 cut(s) 369, 387
BseGI GGATG 5 cut(s) 367, 397, 576, 757, 1195
BseLI CCNNNNNNNGG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 831, 1164
BseRI GAGGAG 1 cut(s) 855
BseSI GKGCMC 2 cut(s) 573, 1071
BseXI GCAGC 5 cut(s) 298, 751, 1166, 1169, 1172
BseYI CCCAGC 1 cut(s) 399
BshFI GGCC 3 cut(s) 463, 1026, 1135
BshNI GGYRCC 2 cut(s) 294, 570
BsiHKCI CYCGRG 1 cut(s) 1118
BsiSI CCGG 2 cut(s) 428, 877
BslFI GGGAC 4 cut(s) 379, 416, 506, 707
BslI CCNNNNNNNGG 1 cut(s) 687
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 4 cut(s) 379, 416, 506, 707
BsmI GAATGC 2 cut(s) 401, 1086
BsnI GGCC 3 cut(s) 463, 1026, 1135
BsoBI CYCGRG 1 cut(s) 1118
Bsp1286I GDGCHC 2 cut(s) 573, 1071
Bsp143I GATC 5 cut(s) 41, 66, 209, 439, 1125
BspACI CCGC 4 cut(s) 193, 1023, 1136, 1172
BspANI GGCC 3 cut(s) 463, 1026, 1135
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 7 cut(s) 43, 84, 135, 296, 367, 572, 860
BspPI GGATC 3 cut(s) 36, 49, 74
BspT107I GGYRCC 2 cut(s) 294, 570
BsrI ACTGG 2 cut(s) 831, 1164
BssECI CCNNGG 2 cut(s) 369, 387
BssMI GATC 5 cut(s) 41, 66, 209, 439, 1125
Bst2UI CCWGG 4 cut(s) 370, 388, 497, 848
Bst4CI ACNGT 1 cut(s) 1078
Bst6I CTCTTC 2 cut(s) 226, 681
BstC8I GCNNGC 2 cut(s) 844, 1084
BstDEI CTNAG 4 cut(s) 200, 270, 592, 915
BstF5I GGATG 5 cut(s) 367, 397, 576, 757, 1195
BstKTI GATC 5 cut(s) 44, 69, 212, 442, 1128
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 41, 66, 209, 439, 1125
BstMWI GCNNNNNNNGC 4 cut(s) 152, 1154, 1160, 1169
BstNI CCWGG 4 cut(s) 370, 388, 497, 848
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 6 cut(s) 368, 386, 426, 495, 846, 875
BstSFI CTRYAG 2 cut(s) 980, 1077
BstSLI GKGCMC 2 cut(s) 573, 1071
BstV1I GCAGC 5 cut(s) 298, 751, 1166, 1169, 1172
BstV2I GAAGAC 2 cut(s) 381, 1047
BstX2I RGATCY 2 cut(s) 41, 66
BstYI RGATCY 2 cut(s) 41, 66
BsuRI GGCC 3 cut(s) 463, 1026, 1135
BtgZI GCGATG 1 cut(s) 437
BtrI CACGTC 1 cut(s) 728
BtsCI GGATG 5 cut(s) 367, 397, 576, 757, 1195
BtsIMutI CAGTG 1 cut(s) 885
Cac8I GCNNGC 2 cut(s) 844, 1084
Cfr13I GGNCC 4 cut(s) 133, 248, 366, 957
Csp6I GTAC 2 cut(s) 295, 669
CviAII CATG 2 cut(s) 112, 687
CviQI GTAC 2 cut(s) 295, 669
DdeI CTNAG 4 cut(s) 200, 270, 592, 915
DpnI GATC 5 cut(s) 43, 68, 211, 441, 1127
DpnII GATC 5 cut(s) 41, 66, 209, 439, 1125
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 1 cut(s) 1024
Eam1104I CTCTTC 2 cut(s) 226, 681
EarI CTCTTC 2 cut(s) 226, 681
Eco47I GGWCC 4 cut(s) 133, 248, 366, 957
Eco57I CTGAAG 2 cut(s) 246, 279
Eco88I CYCGRG 1 cut(s) 1118
EcoO109I RGGNCCY 3 cut(s) 133, 366, 957
EcoRII CCWGG 4 cut(s) 368, 386, 495, 846
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 2 cut(s) 115, 690
FaqI GGGAC 4 cut(s) 379, 416, 506, 707
FatI CATG 2 cut(s) 111, 686
FauI CCCGC 1 cut(s) 200
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 7 cut(s) 312, 765, 1024, 1136, 1155, 1158, 1161
FokI GGATG 5 cut(s) 374, 404, 563, 764, 1202
Fsp4HI GCNGC 7 cut(s) 312, 765, 1024, 1136, 1155, 1158, 1161
FspBI CTAG 2 cut(s) 11, 936
GluI GCNGC 7 cut(s) 312, 765, 1024, 1136, 1155, 1158, 1161
GsaI CCCAGC 1 cut(s) 403
HaeIII GGCC 3 cut(s) 463, 1026, 1135
HapII CCGG 2 cut(s) 428, 877
Hin1II CATG 2 cut(s) 115, 690
HindIII AAGCTT 1 cut(s) 925
HinfI GANTC 4 cut(s) 92, 635, 720, 1052
HpaII CCGG 2 cut(s) 428, 877
HphI GGTGA 4 cut(s) 110, 653, 767, 1054
Hpy166II GTNNAC 2 cut(s) 484, 797
Hpy188I TCNGA 4 cut(s) 66, 259, 342, 537
Hpy188III TCNNGA 8 cut(s) 74, 131, 306, 318, 808, 853, 901, 1120
Hpy8I GTNNAC 2 cut(s) 484, 797
HpyAV CCTTC 5 cut(s) 221, 550, 785, 785, 901
HpyCH4III ACNGT 1 cut(s) 1078
HpyCH4IV ACGT 1 cut(s) 727
HpyCH4V TGCA 4 cut(s) 146, 314, 698, 1086
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 1154, 1160, 1169
HpyF3I CTNAG 4 cut(s) 200, 270, 592, 915
HpySE526I ACGT 1 cut(s) 727
Hsp92II CATG 2 cut(s) 115, 690
KpnI GGTACC 1 cut(s) 298
Kzo9I GATC 5 cut(s) 41, 66, 209, 439, 1125
Lsp1109I GCAGC 5 cut(s) 298, 751, 1166, 1169, 1172
LweI GCATC 1 cut(s) 323
MaeI CTAG 2 cut(s) 11, 936
MaeII ACGT 1 cut(s) 727
MaeIII GTNAC 4 cut(s) 503, 833, 992, 1113
MalI GATC 5 cut(s) 43, 68, 211, 441, 1127
MboI GATC 5 cut(s) 41, 66, 209, 439, 1125
MboII GAAGA 9 cut(s) 243, 272, 386, 608, 668, 916, 957, 1047, 1135
MfeI CAATTG 3 cut(s) 36, 213, 651
MflI RGATCY 2 cut(s) 41, 66
MhlI GDGCHC 2 cut(s) 573, 1071
MluCI AATT 7 cut(s) 27, 36, 213, 651, 812, 817, 1097
MlyI GAGTC 2 cut(s) 729, 1061
MseI TTAA 2 cut(s) 548, 815
MslI CAYNNNNRTG 1 cut(s) 1091
MspI CCGG 2 cut(s) 428, 877
MspR9I CCNGG 6 cut(s) 370, 388, 428, 497, 848, 877
MunI CAATTG 3 cut(s) 36, 213, 651
Mva1269I GAATGC 2 cut(s) 401, 1086
MvaI CCWGG 4 cut(s) 370, 388, 497, 848
MwoI GCNNNNNNNGC 4 cut(s) 152, 1154, 1160, 1169
NciI CCSGG 2 cut(s) 428, 877
NdeII GATC 5 cut(s) 41, 66, 209, 439, 1125
NlaIII CATG 2 cut(s) 115, 690
NlaIV GGNNCC 7 cut(s) 43, 84, 135, 296, 367, 572, 860
NmuCI GTSAC 1 cut(s) 833
NspI RCATGY 1 cut(s) 115
PaeR7I CTCGAG 1 cut(s) 1118
PctI GAATGC 2 cut(s) 401, 1086
PfeI GAWTC 2 cut(s) 92, 635
PkrI GCNGC 7 cut(s) 313, 766, 1025, 1137, 1156, 1159, 1162
PleI GAGTC 2 cut(s) 728, 1060
PpsI GAGTC 2 cut(s) 728, 1060
PpuMI RGGWCCY 3 cut(s) 133, 366, 957
Psp5II RGGWCCY 3 cut(s) 133, 366, 957
Psp6I CCWGG 4 cut(s) 368, 386, 495, 846
PspFI CCCAGC 1 cut(s) 399
PspGI CCWGG 4 cut(s) 368, 386, 495, 846
PspN4I GGNNCC 7 cut(s) 43, 84, 135, 296, 367, 572, 860
PspPI GGNCC 4 cut(s) 133, 248, 366, 957
PspPPI RGGWCCY 3 cut(s) 133, 366, 957
PsuI RGATCY 2 cut(s) 41, 66
RsaI GTAC 2 cut(s) 296, 670
RsaNI GTAC 2 cut(s) 295, 669
RseI CAYNNNNRTG 1 cut(s) 1091
SaqAI TTAA 2 cut(s) 548, 815
SatI GCNGC 7 cut(s) 312, 765, 1024, 1136, 1155, 1158, 1161
Sau3AI GATC 5 cut(s) 41, 66, 209, 439, 1125
Sau96I GGNCC 4 cut(s) 133, 248, 366, 957
SchI GAGTC 2 cut(s) 729, 1061
ScrFI CCNGG 6 cut(s) 370, 388, 428, 497, 848, 877
SduI GDGCHC 2 cut(s) 573, 1071
SfaNI GCATC 1 cut(s) 323
SfcI CTRYAG 2 cut(s) 980, 1077
Sfr274I CTCGAG 1 cut(s) 1118
SinI GGWCC 4 cut(s) 133, 248, 366, 957
SlaI CTCGAG 1 cut(s) 1118
SmiMI CAYNNNNRTG 1 cut(s) 1091
SmlI CTYRAG 1 cut(s) 1118
SmoI CTYRAG 1 cut(s) 1118
Sse9I AATT 7 cut(s) 27, 36, 213, 651, 812, 817, 1097
SsiI CCGC 4 cut(s) 193, 1023, 1136, 1172
SspMI CTAG 2 cut(s) 11, 936
StyD4I CCNGG 6 cut(s) 368, 386, 426, 495, 846, 875
TaaI ACNGT 1 cut(s) 1078
TaiI ACGT 1 cut(s) 730
TaqI TCGA 4 cut(s) 325, 786, 902, 1119
TasI AATT 7 cut(s) 27, 36, 213, 651, 812, 817, 1097
TauI GCSGC 2 cut(s) 1026, 1138
TfiI GAWTC 2 cut(s) 92, 635
Tru1I TTAA 2 cut(s) 548, 815
Tru9I TTAA 2 cut(s) 548, 815
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 5 cut(s) 311, 764, 1154, 1157, 1160
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 3 cut(s) 105, 408, 804
TspRI CASTG 1 cut(s) 892
VpaK11BI GGWCC 4 cut(s) 133, 248, 366, 957
XapI RAATTY 2 cut(s) 27, 817
XceI RCATGY 1 cut(s) 115
XhoI CTCGAG 1 cut(s) 1118
XmiI GTMKAC 1 cut(s) 796
XspI CTAG 2 cut(s) 11, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.