FvH4_c1g00520

NADH dehydrogenase (quinone) activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
contig_1
OG0015812
Physical Location & Seq
Forward (+)
119246 .. 120292
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_c1g00520.t1

Sequence Viewer

Length: 777 bp
ATGTATGGGGGGTCCAGTACACCACCTCCTGGCCCGTCTGAGAATTGGGGACTCTCTTCTTTCTCAATTGCAGGCGAAAGCTCTCACGGTGAGCAGAGGGGTTCGCCTTCGTTGTCGGCGGAGTTTCCCGTTCTTCAGATGCCAACTCCTGAGTTTCAGGGGATGATGGCCGCCTTCGACTTTTCCTCGGCGCCCGTCGTACCGGAAACCCAACTCCTGGGGGAAAGTTCCAAAAGCGCAGTTCCGGAACCTGAAGAGCAGCCTCTACTGGAAGAAGCGGCTCCCAACAGACAGATTCAGGAGTTGCGAAACGATCCCTACTTAGCGAGATCATATAAAAATGCACTGATAAAACAAGAGGAGATTATTGTCGTTATGACACAACTTGTGGACGAACTCGGGATACACATAGCAGACCCCGGGGATATCCGCAAAGGGAATTCGTTTGGATCCTCCCACATGTTCAATCTTTTTTTAGCGGTTTCCCCAGAGATCTTTATCATTAATGCAACCTTCATTTTGCTCATTCATGGAGTTGTATTTAGTACCTCTAAGAAATATGATTATCCACCGTTAGTCAGTAATGTGGGTTGGCTTGGATTACTTAGTGTTGCGCGCCTAGGAGGGCAGCGCGCTTTGGGATGCGGAGTAGCTATTATCGCCCAGCTCCCTAACCTAATGCGGCACCGGGTTCGGACCGCAGGGAACCGTAGCATGGGGGGGGCGTCTAATCCTTTTGCCGCCGCAAGGCTGGCTAATCGTACGCAGCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

259

Amino Acids

27.76

Weight (kDa)

5.76

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015812)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00285
fragaria_vesca FvH4_c1g00520
rosa_chinensis RchiOBHm_MTg0499211
rosa_laevigata RLG00000000016 RLG00000000081 RLG00000000123 RLG00000000367 RLG00000017272
rosa_multiflora Rmu_sc0000486.1_g000004
rosa_roxburghii Rroxscaffold_2G00119530 Rroxscaffold_2G00119540 Rroxscaffold_6G00392870
rosa_samantha Rh2BG107500 Rh2CG109300
rosa_wichuraiana Rw3G029490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 190, 684
AccB7I CCANNNNNTGG 2 cut(s) 29, 217
AccII CGCG 2 cut(s) 616, 633
AccIII TCCGGA 1 cut(s) 244
AclWI GGATC 3 cut(s) 308, 444, 457
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 2 cut(s) 119, 273
AcyI GRCGYC 2 cut(s) 191, 725
AfaI GTAC 4 cut(s) 19, 201, 547, 763
AfiI CCNNNNNNNGG 4 cut(s) 29, 217, 715, 747
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 1 cut(s) 466
AjnI CCWGG 2 cut(s) 28, 216
AluBI AGCT 3 cut(s) 81, 653, 667
AluI AGCT 3 cut(s) 81, 653, 667
AlwI GGATC 3 cut(s) 308, 444, 457
Ama87I CYCGRG 2 cut(s) 398, 419
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 2 cut(s) 31, 168
ApeKI GCWGC 3 cut(s) 259, 628, 766
ApoI RAATTY 1 cut(s) 439
AseI ATTAAT 1 cut(s) 504
AspA2I CCTAGG 1 cut(s) 619
AspLEI GCGC 6 cut(s) 193, 239, 616, 618, 633, 635
AspS9I GGNCC 3 cut(s) 12, 32, 696
AsuC2I CCSGG 3 cut(s) 420, 421, 689
AsuHPI GGTGA 1 cut(s) 101
AvaI CYCGRG 2 cut(s) 398, 419
AvaII GGWCC 2 cut(s) 12, 696
AvrII CCTAGG 1 cut(s) 619
BamHI GGATCC 1 cut(s) 449
BanI GGYRCC 2 cut(s) 190, 684
BbvI GCAGC 2 cut(s) 271, 640
BccI CCATC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 2 cut(s) 30, 218
BciVI GTATCC 1 cut(s) 396
BcnI CCSGG 3 cut(s) 420, 421, 689
BfaI CTAG 1 cut(s) 620
BfoI RGCGCY 1 cut(s) 194
BfuI GTATCC 1 cut(s) 396
BglII AGATCT 1 cut(s) 492
BisI GCNGC 8 cut(s) 171, 260, 279, 629, 683, 741, 744, 767
BlnI CCTAGG 1 cut(s) 619
BlsI GCNGC 8 cut(s) 172, 261, 280, 630, 684, 742, 745, 768
Bme1390I CCNGG 5 cut(s) 30, 218, 420, 421, 689
Bme18I GGWCC 2 cut(s) 12, 696
BmeT110I CYCGRG 2 cut(s) 398, 419
BmgT120I GGNCC 3 cut(s) 12, 32, 696
BmiI GGNNCC 7 cut(s) 13, 192, 249, 282, 451, 686, 707
BmrFI CCNGG 5 cut(s) 30, 218, 420, 421, 689
BmsI GCATC 2 cut(s) 129, 632
BpuMI CCSGG 3 cut(s) 420, 421, 689
BsaBI GATNNNNATC 1 cut(s) 497
BsaHI GRCGYC 2 cut(s) 191, 725
BsaJI CCNNGG 6 cut(s) 186, 217, 418, 419, 420, 619
BsaWI WCCGGW 2 cut(s) 202, 244
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 217, 715, 747
Bse1I ACTGG 2 cut(s) 15, 273
Bse8I GATNNNNATC 1 cut(s) 497
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 2 cut(s) 30, 218
BseDI CCNNGG 6 cut(s) 186, 217, 418, 419, 420, 619
BseGI GGATG 2 cut(s) 168, 647
BseJI GATNNNNATC 1 cut(s) 497
BseLI CCNNNNNNNGG 4 cut(s) 29, 217, 715, 747
BseMII CTCAG 2 cut(s) 30, 141
BseNI ACTGG 2 cut(s) 15, 273
BsePI GCGCGC 2 cut(s) 614, 631
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 271, 640
BseYI CCCAGC 1 cut(s) 663
Bsh1236I CGCG 2 cut(s) 616, 633
BshFI GGCC 2 cut(s) 33, 170
BshNI GGYRCC 2 cut(s) 190, 684
BsiHKCI CYCGRG 2 cut(s) 398, 419
BsiSI CCGG 4 cut(s) 203, 245, 420, 688
BsiWI CGTACG 1 cut(s) 761
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 4 cut(s) 29, 217, 715, 747
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 2 cut(s) 33, 170
BsoBI CYCGRG 2 cut(s) 398, 419
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 4 cut(s) 313, 329, 449, 492
BspANI GGCC 2 cut(s) 33, 170
BspCNI CTCAG 2 cut(s) 31, 142
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 616, 633
BspLI GGNNCC 7 cut(s) 13, 192, 249, 282, 451, 686, 707
BspPI GGATC 3 cut(s) 308, 444, 457
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 2 cut(s) 190, 684
BsrI ACTGG 2 cut(s) 15, 273
BssECI CCNNGG 6 cut(s) 186, 217, 418, 419, 420, 619
BssHII GCGCGC 2 cut(s) 614, 631
BssMI GATC 4 cut(s) 313, 329, 449, 492
BssNI GRCGYC 2 cut(s) 191, 725
BssT1I CCWWGG 1 cut(s) 619
Bst2UI CCWGG 2 cut(s) 30, 218
Bst4CI ACNGT 3 cut(s) 89, 573, 710
Bst6I CTCTTC 2 cut(s) 61, 249
BstACI GRCGYC 2 cut(s) 191, 725
BstC8I GCNNGC 5 cut(s) 73, 616, 633, 753, 771
BstDEI CTNAG 5 cut(s) 39, 150, 322, 552, 605
BstF5I GGATG 2 cut(s) 168, 647
BstFNI CGCG 2 cut(s) 616, 633
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 6 cut(s) 193, 239, 616, 618, 633, 635
BstKTI GATC 4 cut(s) 316, 332, 452, 495
BstMBI GATC 4 cut(s) 313, 329, 449, 492
BstMWI GCNNNNNNNGC 2 cut(s) 659, 752
BstNI CCWGG 2 cut(s) 30, 218
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 5 cut(s) 28, 216, 418, 419, 687
BstUI CGCG 2 cut(s) 616, 633
BstV1I GCAGC 2 cut(s) 271, 640
BstX2I RGATCY 2 cut(s) 449, 492
BstYI RGATCY 2 cut(s) 449, 492
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 33, 170
BtsCI GGATG 2 cut(s) 168, 647
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 5 cut(s) 73, 616, 633, 753, 771
CfoI GCGC 6 cut(s) 193, 239, 616, 618, 633, 635
Cfr13I GGNCC 3 cut(s) 12, 32, 696
Cfr9I CCCGGG 1 cut(s) 419
CpoI CGGWCCG 1 cut(s) 696
CseI GACGC 1 cut(s) 714
Csp6I GTAC 4 cut(s) 18, 200, 546, 762
CspI CGGWCCG 1 cut(s) 696
CviAII CATG 3 cut(s) 460, 530, 715
CviQI GTAC 4 cut(s) 18, 200, 546, 762
DdeI CTNAG 5 cut(s) 39, 150, 322, 552, 605
DinI GGCGCC 1 cut(s) 192
DpnI GATC 4 cut(s) 315, 331, 451, 494
DpnII GATC 4 cut(s) 313, 329, 449, 492
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 61, 249
EarI CTCTTC 2 cut(s) 61, 249
EciI GGCGGA 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 619
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 12, 696
Eco57I CTGAAG 2 cut(s) 119, 273
Eco88I CYCGRG 2 cut(s) 398, 419
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 28, 216
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 619
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 619
FaeI CATG 3 cut(s) 463, 533, 718
FaiI YATR 9 cut(s) 6, 334, 336, 377, 410, 461, 531, 561, 716
FaqI GGGAC 1 cut(s) 63
FatI CATG 3 cut(s) 459, 529, 714
Fnu4HI GCNGC 8 cut(s) 171, 260, 279, 629, 683, 741, 744, 767
FokI GGATG 2 cut(s) 175, 654
Fsp4HI GCNGC 8 cut(s) 171, 260, 279, 629, 683, 741, 744, 767
FspBI CTAG 1 cut(s) 620
GlaI GCGC 6 cut(s) 192, 238, 615, 617, 632, 634
GluI GCNGC 8 cut(s) 171, 260, 279, 629, 683, 741, 744, 767
GsaI CCCAGC 1 cut(s) 667
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 33, 170
HapII CCGG 4 cut(s) 203, 245, 420, 688
HgaI GACGC 1 cut(s) 714
HhaI GCGC 6 cut(s) 193, 239, 616, 618, 633, 635
Hin1I GRCGYC 2 cut(s) 191, 725
Hin1II CATG 3 cut(s) 463, 533, 718
Hin6I GCGC 6 cut(s) 191, 237, 614, 616, 631, 633
HinP1I GCGC 6 cut(s) 191, 237, 614, 616, 631, 633
HinfI GANTC 2 cut(s) 51, 295
HpaII CCGG 4 cut(s) 203, 245, 420, 688
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 20, 391
Hpy188I TCNGA 3 cut(s) 40, 138, 696
Hpy188III TCNNGA 4 cut(s) 149, 245, 299, 400
Hpy8I GTNNAC 2 cut(s) 20, 391
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 3 cut(s) 117, 184, 523
HpyCH4III ACNGT 3 cut(s) 89, 573, 710
HpyCH4V TGCA 3 cut(s) 71, 344, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 752
HpyF3I CTNAG 5 cut(s) 39, 150, 322, 552, 605
Hsp92I GRCGYC 2 cut(s) 191, 725
Hsp92II CATG 3 cut(s) 463, 533, 718
HspAI GCGC 6 cut(s) 191, 237, 614, 616, 631, 633
KasI GGCGCC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 244
Kzo9I GATC 4 cut(s) 313, 329, 449, 492
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 2 cut(s) 286, 672
Lsp1109I GCAGC 2 cut(s) 271, 640
LweI GCATC 2 cut(s) 129, 632
MaeI CTAG 1 cut(s) 620
MalI GATC 4 cut(s) 315, 331, 451, 494
MboI GATC 4 cut(s) 313, 329, 449, 492
MboII GAAGA 4 cut(s) 48, 125, 266, 284
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 2 cut(s) 449, 492
MluCI AATT 3 cut(s) 43, 66, 439
Mly113I GGCGCC 1 cut(s) 191
MlyI GAGTC 1 cut(s) 45
MnlI CCTC 8 cut(s) 36, 90, 196, 273, 352, 463, 559, 617
MroI TCCGGA 1 cut(s) 244
MseI TTAA 1 cut(s) 504
MspI CCGG 4 cut(s) 203, 245, 420, 688
MspR9I CCNGG 5 cut(s) 30, 218, 420, 421, 689
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 2 cut(s) 30, 218
MvnI CGCG 2 cut(s) 616, 633
MwoI GCNNNNNNNGC 2 cut(s) 659, 752
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 3 cut(s) 420, 421, 689
NdeII GATC 4 cut(s) 313, 329, 449, 492
NlaIII CATG 3 cut(s) 463, 533, 718
NlaIV GGNNCC 7 cut(s) 13, 192, 249, 282, 451, 686, 707
NmeAIII GCCGAG 1 cut(s) 167
NspI RCATGY 1 cut(s) 463
PauI GCGCGC 2 cut(s) 614, 631
PciI ACATGT 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 295
Pfl23II CGTACG 1 cut(s) 761
PflMI CCANNNNNTGG 2 cut(s) 29, 217
PkrI GCNGC 8 cut(s) 172, 261, 280, 630, 684, 742, 745, 768
PleI GAGTC 1 cut(s) 45
PluTI GGCGCC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 45
PscI ACATGT 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 28, 216
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 2 cut(s) 28, 216
PspLI CGTACG 1 cut(s) 761
PspN4I GGNNCC 7 cut(s) 13, 192, 249, 282, 451, 686, 707
PspPI GGNCC 3 cut(s) 12, 32, 696
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PsuI RGATCY 2 cut(s) 449, 492
PteI GCGCGC 2 cut(s) 614, 631
RsaI GTAC 4 cut(s) 19, 201, 547, 763
RsaNI GTAC 4 cut(s) 18, 200, 546, 762
Rsr2I CGGWCCG 1 cut(s) 696
RsrII CGGWCCG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 8 cut(s) 171, 260, 279, 629, 683, 741, 744, 767
Sau3AI GATC 4 cut(s) 313, 329, 449, 492
Sau96I GGNCC 3 cut(s) 12, 32, 696
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 5 cut(s) 30, 218, 420, 421, 689
SetI ASST 8 cut(s) 28, 83, 253, 515, 551, 655, 669, 678
SfaNI GCATC 2 cut(s) 129, 632
SfoI GGCGCC 1 cut(s) 192
SinI GGWCC 2 cut(s) 12, 696
SmaI CCCGGG 1 cut(s) 421
Sse9I AATT 3 cut(s) 43, 66, 439
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 1 cut(s) 620
StyD4I CCNGG 5 cut(s) 28, 216, 418, 419, 687
StyI CCWWGG 1 cut(s) 619
TaaI ACNGT 3 cut(s) 89, 573, 710
TaqI TCGA 1 cut(s) 177
TasI AATT 3 cut(s) 43, 66, 439
TatI WGTACW 1 cut(s) 17
TauI GCSGC 5 cut(s) 173, 281, 685, 743, 746
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 1 cut(s) 351
TseI GCWGC 3 cut(s) 259, 628, 766
TspDTI ATGAA 2 cut(s) 505, 518
TspMI CCCGGG 1 cut(s) 419
TspRI CASTG 1 cut(s) 351
Van91I CCANNNNNTGG 2 cut(s) 29, 217
VpaK11BI GGWCC 2 cut(s) 12, 696
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 463
XmaI CCCGGG 1 cut(s) 419
XmaJI CCTAGG 1 cut(s) 619
XspI CTAG 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.