Rroxscaffold_6G00392870

NADH dehydrogenase (quinone) activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015812
Physical Location & Seq
Reverse (-)
12663831 .. 12665335
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392870.1

Sequence Viewer

Length: 723 bp
ATGCCTCAACCGGATAAATTCACTTATTTCACACAATTCTTCCGGTCATGCCTTTTCCTCTTTACTTTCTATATTCCCATATGCAATGATGGAGATGGATTCCTTGGGATCAGCAGAATTCTAAAACTACGGAACCGACCGGTTTCACAGCGGGGGAACAACACTCGGAGCAACGACCCCAACAGTTTGGAAGAGATTTTGAGAAAAGGTTTTAGTACGGTGTATCCTATATGTACTCTAGTTTATTCGAAGCGGCAAAATGGTGTAACGTCGTCGACTTATTGGGAAAAGGGAAGAAATTATTGGGAAAAGGGAAGAAATTATTGGGAAAAGGGAAGAAAATGCGCCGGGACTACTCCAGTTTATTTGAAGTCGCAAAATGGTAATTCGTTCGGATCCTCCCACATGTTCAATCTTTTTTTAGCGGTTTCCCCAAAGATCTTTATCATTAATGCAACCTTCATTTTGCTCATTCATGGAGTTGTATTTAGTACCTCTAAGAAATATGATTATCCACCGTTAGTCGGTAATGTGGGTTGGCTTGGATTACTTAGTGTTGCGCGCCTAGGAGGGCGGCGCGCTTTGGGATGCGGAGTAGCTATTATCGCCCAGCTCCCTAACCTAATGCGGCACCGGGTTCGGACCGCAGGGAACCGTAGCATGGGGGGGGCATCTAATCCTTTTGCCGCCGCAAGGCCGGCTAATCGTACGCAGCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

240

Amino Acids

26.94

Weight (kDa)

10.4

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YMF19 PF02326 2 - 71 3.1e-15 Plant ATP synthase F0
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015812)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00285
fragaria_vesca FvH4_c1g00520
rosa_chinensis RchiOBHm_MTg0499211
rosa_laevigata RLG00000000016 RLG00000000081 RLG00000000123 RLG00000000367 RLG00000017272
rosa_multiflora Rmu_sc0000486.1_g000004
rosa_roxburghii Rroxscaffold_2G00119530 Rroxscaffold_2G00119540 Rroxscaffold_6G00392870
rosa_samantha Rh2BG107500 Rh2CG109300
rosa_wichuraiana Rw3G029490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 630
AccI GTMKAC 1 cut(s) 275
AccII CGCG 2 cut(s) 562, 579
AciI CCGC 9 cut(s) 151, 253, 425, 574, 591, 628, 645, 687, 690
AclWI GGATC 3 cut(s) 116, 390, 403
AcsI RAATTY 2 cut(s) 17, 117
AfaI GTAC 4 cut(s) 217, 235, 493, 709
AfiI CCNNNNNNNGG 3 cut(s) 524, 661, 693
AflIII ACRYGT 1 cut(s) 405
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 2 cut(s) 370, 412
AjuI GAANNNNNNNTTGG 4 cut(s) 286, 307, 318, 339
AluBI AGCT 2 cut(s) 599, 613
AluI AGCT 2 cut(s) 599, 613
AlwI GGATC 3 cut(s) 116, 390, 403
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 1 cut(s) 712
ApoI RAATTY 2 cut(s) 17, 117
AseI ATTAAT 1 cut(s) 450
AsiGI ACCGGT 1 cut(s) 139
AspA2I CCTAGG 1 cut(s) 565
AspLEI GCGC 5 cut(s) 347, 562, 564, 579, 581
AspS9I GGNCC 1 cut(s) 642
AsuC2I CCSGG 2 cut(s) 349, 635
AsuII TTCGAA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 642
AvrII CCTAGG 1 cut(s) 565
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 630
BccI CCATC 2 cut(s) 83, 89
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciVI GTATCC 1 cut(s) 234
BcnI CCSGG 2 cut(s) 349, 635
BfaI CTAG 2 cut(s) 239, 566
BfuI GTATCC 1 cut(s) 234
BglII AGATCT 1 cut(s) 438
BisI GCNGC 6 cut(s) 254, 575, 629, 687, 690, 713
BlnI CCTAGG 1 cut(s) 565
BlsI GCNGC 6 cut(s) 255, 576, 630, 688, 691, 714
Bme1390I CCNGG 2 cut(s) 349, 635
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 4 cut(s) 134, 397, 632, 653
BmrFI CCNGG 2 cut(s) 349, 635
BmsI GCATC 2 cut(s) 578, 680
BpmI CTGGAG 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 349, 635
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 103, 565
BsaWI WCCGGW 3 cut(s) 10, 42, 139
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 661, 693
Bse118I RCCGGY 2 cut(s) 139, 697
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 103, 565
BseGI GGATG 1 cut(s) 593
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 3 cut(s) 524, 661, 693
BseMI GCAATG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 359
BsePI GCGCGC 2 cut(s) 560, 577
BseYI CCCAGC 1 cut(s) 609
Bsh1236I CGCG 2 cut(s) 562, 579
Bsh1285I CGRYCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 1 cut(s) 630
BshTI ACCGGT 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 140
BsiSI CCGG 6 cut(s) 11, 43, 140, 348, 634, 698
BsiWI CGTACG 1 cut(s) 707
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 3 cut(s) 524, 661, 693
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 1 cut(s) 697
Bsp119I TTCGAA 1 cut(s) 248
Bsp143I GATC 3 cut(s) 108, 395, 438
BspACI CCGC 9 cut(s) 151, 253, 425, 574, 591, 628, 645, 687, 690
BspANI GGCC 1 cut(s) 697
BspFNI CGCG 2 cut(s) 562, 579
BspLI GGNNCC 4 cut(s) 134, 397, 632, 653
BspPI GGATC 3 cut(s) 116, 390, 403
BspT104I TTCGAA 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 91
BsrFI RCCGGY 2 cut(s) 139, 697
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 2 cut(s) 139, 697
BssECI CCNNGG 2 cut(s) 103, 565
BssHII GCGCGC 2 cut(s) 560, 577
BssMI GATC 3 cut(s) 108, 395, 438
BssT1I CCWWGG 2 cut(s) 103, 565
Bst4CI ACNGT 4 cut(s) 185, 220, 519, 656
Bst6I CTCTTC 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 4 cut(s) 562, 579, 699, 717
BstDEI CTNAG 2 cut(s) 498, 551
BstF5I GGATG 1 cut(s) 593
BstFNI CGCG 2 cut(s) 562, 579
BstHHI GCGC 5 cut(s) 347, 562, 564, 579, 581
BstKTI GATC 3 cut(s) 111, 398, 441
BstMBI GATC 3 cut(s) 108, 395, 438
BstMCI CGRYCG 1 cut(s) 140
BstMWI GCNNNNNNNGC 2 cut(s) 605, 698
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 347, 633
BstUI CGCG 2 cut(s) 562, 579
BstX2I RGATCY 2 cut(s) 395, 438
BstXI CCANNNNNNTGG 1 cut(s) 187
BstYI RGATCY 2 cut(s) 395, 438
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 1 cut(s) 697
BtsCI GGATG 1 cut(s) 593
Cac8I GCNNGC 4 cut(s) 562, 579, 699, 717
CfoI GCGC 5 cut(s) 347, 562, 564, 579, 581
Cfr10I RCCGGY 2 cut(s) 139, 697
Cfr13I GGNCC 1 cut(s) 642
CpoI CGGWCCG 1 cut(s) 642
Csp6I GTAC 4 cut(s) 216, 234, 492, 708
CspAI ACCGGT 1 cut(s) 139
CspI CGGWCCG 1 cut(s) 642
CviAII CATG 4 cut(s) 48, 406, 476, 661
CviJI RGCY 6 cut(s) 541, 599, 613, 697, 701, 719
CviKI_1 RGCY 6 cut(s) 541, 599, 613, 697, 701, 719
CviQI GTAC 4 cut(s) 216, 234, 492, 708
DdeI CTNAG 2 cut(s) 498, 551
DpnI GATC 3 cut(s) 110, 397, 440
DpnII GATC 3 cut(s) 108, 395, 438
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 103, 565
Eco47I GGWCC 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 103, 565
ErhI CCWWGG 2 cut(s) 103, 565
FaeI CATG 4 cut(s) 51, 409, 479, 664
FaqI GGGAC 1 cut(s) 364
FatI CATG 4 cut(s) 47, 405, 475, 660
FauI CCCGC 1 cut(s) 144
FauNDI CATATG 1 cut(s) 80
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 6 cut(s) 254, 575, 629, 687, 690, 713
FokI GGATG 1 cut(s) 600
Fsp4HI GCNGC 6 cut(s) 254, 575, 629, 687, 690, 713
FspBI CTAG 2 cut(s) 239, 566
GlaI GCGC 5 cut(s) 346, 561, 563, 578, 580
GluI GCNGC 6 cut(s) 254, 575, 629, 687, 690, 713
GsaI CCCAGC 1 cut(s) 613
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 697
HapII CCGG 6 cut(s) 11, 43, 140, 348, 634, 698
HhaI GCGC 5 cut(s) 347, 562, 564, 579, 581
Hin1II CATG 4 cut(s) 51, 409, 479, 664
Hin6I GCGC 5 cut(s) 345, 560, 562, 577, 579
HinP1I GCGC 5 cut(s) 345, 560, 562, 577, 579
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 1 cut(s) 99
HpaII CCGG 6 cut(s) 11, 43, 140, 348, 634, 698
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 3 cut(s) 168, 395, 642
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 2 cut(s) 274, 277
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 4 cut(s) 185, 220, 519, 656
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 2 cut(s) 84, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 698
HpyF3I CTNAG 2 cut(s) 498, 551
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 4 cut(s) 51, 409, 479, 664
HspAI GCGC 5 cut(s) 345, 560, 562, 577, 579
KroI GCCGGC 1 cut(s) 697
KroNI GCCGGC 1 cut(s) 699
Kzo9I GATC 3 cut(s) 108, 395, 438
LmnI GCTCC 2 cut(s) 168, 618
LweI GCATC 2 cut(s) 578, 680
MaeI CTAG 2 cut(s) 239, 566
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 110, 397, 440
MboI GATC 3 cut(s) 108, 395, 438
MboII GAAGA 5 cut(s) 31, 203, 306, 327, 348
MflI RGATCY 2 cut(s) 395, 438
MluCI AATT 6 cut(s) 17, 35, 117, 298, 319, 385
MnlI CCTC 5 cut(s) 15, 68, 409, 505, 563
MroNI GCCGGC 1 cut(s) 697
MseI TTAA 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 6 cut(s) 11, 43, 140, 348, 634, 698
MspR9I CCNGG 2 cut(s) 349, 635
MvnI CGCG 2 cut(s) 562, 579
MwoI GCNNNNNNNGC 2 cut(s) 605, 698
NaeI GCCGGC 1 cut(s) 699
NciI CCSGG 2 cut(s) 349, 635
NdeI CATATG 1 cut(s) 80
NdeII GATC 3 cut(s) 108, 395, 438
NgoMIV GCCGGC 1 cut(s) 697
NlaIII CATG 4 cut(s) 51, 409, 479, 664
NlaIV GGNNCC 4 cut(s) 134, 397, 632, 653
NspI RCATGY 1 cut(s) 409
NspV TTCGAA 1 cut(s) 248
PauI GCGCGC 2 cut(s) 560, 577
PciI ACATGT 1 cut(s) 405
PdiI GCCGGC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 99
Pfl23II CGTACG 1 cut(s) 707
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 6 cut(s) 255, 576, 630, 688, 691, 714
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 609
PspLI CGTACG 1 cut(s) 707
PspN4I GGNNCC 4 cut(s) 134, 397, 632, 653
PspPI GGNCC 1 cut(s) 642
PsuI RGATCY 2 cut(s) 395, 438
PteI GCGCGC 2 cut(s) 560, 577
RsaI GTAC 4 cut(s) 217, 235, 493, 709
RsaNI GTAC 4 cut(s) 216, 234, 492, 708
Rsr2I CGGWCCG 1 cut(s) 642
RsrII CGGWCCG 1 cut(s) 642
SalI GTCGAC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 6 cut(s) 254, 575, 629, 687, 690, 713
Sau3AI GATC 3 cut(s) 108, 395, 438
Sau96I GGNCC 1 cut(s) 642
ScrFI CCNGG 2 cut(s) 349, 635
SetI ASST 7 cut(s) 211, 272, 461, 497, 601, 615, 624
SfaNI GCATC 2 cut(s) 578, 680
SfuI TTCGAA 1 cut(s) 248
SinI GGWCC 1 cut(s) 642
Sse9I AATT 6 cut(s) 17, 35, 117, 298, 319, 385
SsiI CCGC 9 cut(s) 151, 253, 425, 574, 591, 628, 645, 687, 690
SspMI CTAG 2 cut(s) 239, 566
StyD4I CCNGG 2 cut(s) 347, 633
StyI CCWWGG 2 cut(s) 103, 565
TaaI ACNGT 4 cut(s) 185, 220, 519, 656
TaiI ACGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 248, 275
TasI AATT 6 cut(s) 17, 35, 117, 298, 319, 385
TatI WGTACW 1 cut(s) 233
TauI GCSGC 5 cut(s) 256, 577, 631, 689, 692
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseI GCWGC 1 cut(s) 712
TspDTI ATGAA 2 cut(s) 451, 464
TspGWI ACGGA 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 642
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 2 cut(s) 17, 117
XceI RCATGY 1 cut(s) 409
XmaJI CCTAGG 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 2 cut(s) 239, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.