FvH4_c3g00170

50S ribosomal protein L2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
contig_3
OG0003897
Physical Location & Seq
Forward (+)
42898 .. 43816
919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_c3g00170.t1

Sequence Viewer

Length: 597 bp
ATGGAGAAGACAAAATTGTTTCAAGCACCTACAGAAGCGCCCCTTGTTTCAAAGAGAGGAGGACGGATATCATCATCTACATCCAGAAAGCCGTATGCTTTGGAAGAAGCTTGTACAGTTTGGGAAGGGGTTTTGATTGATCAAAAAGAAGAATCTACTTCAACCGATATGCCCTTAGGCACGGCCATACATAACATAGAAATCACACTTGGAAAGGGTGGACAATTAGCTAGAGCAGCAGGTGCTGTAGCGAAACTGATTGCAAAAGAGGGGAAATCGGCCACATTAAAATTACCTTCTGGGGAGGTCCGTTTGATATCCAAAAACTGCTCAGCGACAGTCGGACAAGTGGGGAATGTTGGGGTGAACCAGAAAAGTTTGGGTAAAGCCGGATCGAAATGTTGGCTAGGTAAGCGTCCTGTAGTAAGAGGAGTAGTTATGAACCCTGTAGACCATCCCCATGGGGGTGGTGAAGGGAGGGCCCCAATTGGTAGAAAAAAACCCGCAACCCCTTGGGGTTATCCTGCACTTGGAAGAAGAAGTAGAAAAAGGAATAAATATAGTGATAATTTGATTCTTCGTCGCCGTAGTAAATAG

Protein Analysis

199

Amino Acids

21.18

Weight (kDa)

10.42

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L2_C PF03947 56 - 173 6.7e-50 Ribosomal Proteins L2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 230
AccI GTMKAC 1 cut(s) 450
AciI CCGC 1 cut(s) 504
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 2 cut(s) 183, 279
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 464, 530
AgsI TTSAA 3 cut(s) 23, 51, 162
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 2 cut(s) 110, 230
AluI AGCT 2 cut(s) 110, 230
AlwI GGATC 1 cut(s) 400
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 3 cut(s) 183, 279, 480
ApaI GGGCCC 1 cut(s) 484
ApeKI GCWGC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 307, 480, 481
AsuHPI GGTGA 2 cut(s) 376, 482
AvaII GGWCC 1 cut(s) 307
AxyI CCTNAGG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 248
BccI CCATC 1 cut(s) 462
BceAI ACGGC 3 cut(s) 76, 198, 570
BclI TGATCA 1 cut(s) 139
BfaI CTAG 2 cut(s) 231, 407
BfmI CTRYAG 4 cut(s) 30, 246, 420, 447
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 230
BisI GCNGC 1 cut(s) 237
BlpI GCTNAGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 3 cut(s) 307, 480, 481
BmiI GGNNCC 2 cut(s) 482, 483
BpiI GAAGAC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 460, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 530
Bse21I CCTNAGG 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 460, 512
BseGI GGATG 2 cut(s) 80, 454
BseLI CCNNNNNNNGG 2 cut(s) 464, 530
BseMII CTCAG 1 cut(s) 345
BseRI GAGGAG 2 cut(s) 72, 444
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 3 cut(s) 185, 281, 482
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 464, 530
BsnI GGCC 3 cut(s) 185, 281, 482
Bsp120I GGGCCC 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 113
Bsp143I GATC 2 cut(s) 139, 392
Bsp1720I GCTNAGC 1 cut(s) 331
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 1 cut(s) 504
BspANI GGCC 3 cut(s) 185, 281, 482
BspCNI CTCAG 1 cut(s) 344
BspLI GGNNCC 2 cut(s) 482, 483
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 400
BsrGI TGTACA 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 460, 512
BssMI GATC 2 cut(s) 139, 392
BssT1I CCWWGG 2 cut(s) 460, 512
Bst4CI ACNGT 2 cut(s) 118, 340
BstAPI GCANNNNNTGC 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 113
BstDEI CTNAG 2 cut(s) 175, 331
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 80, 454
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 142, 395
BstMBI GATC 2 cut(s) 139, 392
BstMWI GCNNNNNNNGC 3 cut(s) 236, 242, 412
BstSFI CTRYAG 4 cut(s) 30, 246, 420, 447
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 14
BstXI CCANNNNNNTGG 2 cut(s) 461, 467
Bsu36I CCTNAGG 1 cut(s) 175
BsuRI GGCC 3 cut(s) 185, 281, 482
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 80, 454
BveI ACCTGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 307, 480, 481
CseI GACGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 114
CviAII CATG 1 cut(s) 461
CviJI RGCY 8 cut(s) 91, 110, 185, 230, 281, 389, 406, 482
CviKI_1 RGCY 8 cut(s) 91, 110, 185, 230, 281, 389, 406, 482
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 2 cut(s) 175, 331
DpnI GATC 2 cut(s) 141, 394
DpnII GATC 2 cut(s) 139, 392
EaeI YGGCCR 2 cut(s) 183, 279
Eco130I CCWWGG 2 cut(s) 460, 512
Eco24I GRGCYC 1 cut(s) 484
Eco32I GATATC 2 cut(s) 69, 318
Eco47I GGWCC 1 cut(s) 307
Eco81I CCTNAGG 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 480, 481
EcoRV GATATC 2 cut(s) 69, 318
EcoT14I CCWWGG 2 cut(s) 460, 512
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 2 cut(s) 460, 512
FaeI CATG 1 cut(s) 464
FaiI YATR 8 cut(s) 96, 170, 188, 192, 197, 440, 462, 561
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FatI CATG 1 cut(s) 460
FauI CCCGC 1 cut(s) 511
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 2 cut(s) 67, 441
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 1 cut(s) 237
FspBI CTAG 2 cut(s) 231, 407
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 237
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 185, 281, 482
HapII CCGG 1 cut(s) 390
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 40
Hin1II CATG 1 cut(s) 464
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 2 cut(s) 152, 574
HpaII CCGG 1 cut(s) 390
HphI GGTGA 2 cut(s) 376, 482
Hpy166II GTNNAC 3 cut(s) 221, 367, 451
Hpy188I TCNGA 1 cut(s) 344
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 3 cut(s) 221, 367, 451
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 3 cut(s) 119, 306, 467
HpyCH4III ACNGT 2 cut(s) 118, 340
HpyCH4V TGCA 2 cut(s) 263, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 242, 412
HpyF3I CTNAG 2 cut(s) 175, 331
Hsp92II CATG 1 cut(s) 464
HspAI GCGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 139, 392
LpnPI CCDG 8 cut(s) 97, 225, 285, 383, 403, 432, 459, 537
Lsp1109I GCAGC 1 cut(s) 248
MaeI CTAG 2 cut(s) 231, 407
MalI GATC 2 cut(s) 141, 394
MboI GATC 2 cut(s) 139, 392
MboII GAAGA 6 cut(s) 19, 116, 161, 546, 549, 569
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 5 cut(s) 14, 224, 290, 486, 568
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 6 cut(s) 50, 53, 262, 298, 422, 471
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 2 cut(s) 459, 465
MspI CCGG 1 cut(s) 390
MunI CAATTG 1 cut(s) 486
MwoI GCNNNNNNNGC 3 cut(s) 236, 242, 412
NcoI CCATGG 1 cut(s) 460
NdeII GATC 2 cut(s) 139, 392
NlaIII CATG 1 cut(s) 464
NlaIV GGNNCC 2 cut(s) 482, 483
PaqCI CACCTGC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 152, 574
PkrI GCNGC 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 482, 483
PspOMI GGGCCC 1 cut(s) 480
PspPI GGNCC 3 cut(s) 307, 480, 481
PstNI CAGNNNCTG 1 cut(s) 245
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 2 cut(s) 459, 465
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 2 cut(s) 139, 392
Sau96I GGNCC 3 cut(s) 307, 480, 481
SduI GDGCHC 1 cut(s) 484
SetI ASST 7 cut(s) 31, 112, 232, 244, 298, 309, 412
SfcI CTRYAG 4 cut(s) 30, 246, 420, 447
SinI GGWCC 1 cut(s) 307
SmiMI CAYNNNNRTG 2 cut(s) 459, 465
Sse9I AATT 5 cut(s) 14, 224, 290, 486, 568
SsiI CCGC 1 cut(s) 504
SspMI CTAG 2 cut(s) 231, 407
StyI CCWWGG 2 cut(s) 460, 512
TaaI ACNGT 2 cut(s) 118, 340
TaqI TCGA 1 cut(s) 395
TasI AATT 5 cut(s) 14, 224, 290, 486, 568
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 2 cut(s) 152, 574
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 455
TspGWI ACGGA 2 cut(s) 79, 299
VpaK11BI GGWCC 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 2 cut(s) 231, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.