RLG00000000053

50S ribosomal protein L2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0003897
Physical Location & Seq
Reverse (-)
312008 .. 312639
632 bp
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UTR
Exon/CDS
Intron
RLM00000000053

Sequence Viewer

Length: 576 bp
ATGAGTTATTCGGCAGATTTTGATTCCTATATATCCACTCATGTGATATCATCATCTACATCCAGAAAGCCGTATGCTTTGGAAGAAGCTTGTACAGTTTGGGAAGGGGTTTTGATTGATCAAAAAGAAGAATCTACTTCAACCGATATGCCCTTAGGCACGGCCATACATAACATAGAAATCACACTTGGAAAGGGTGGACAATTAGCTAGAGCAGCAGGTGCTGTAGCGAAACTGATTGCAAAAGAGGGGAAATCGGCCACATTAAAATTACCTTCTGGGGAGGTCCGTTTGATATCCAAAAACTGCTCAGCAACAGTCGGACAAGTGGGGAATGTTGGGGTGAACCAGAAAAGTTTGGGTAAAGCCGGAGCTAAATGTTGGCTAGGTAAGCGTCCTGTAGTAAGAGGAGTAGTTATGAACCCTGTAGACCATCCCCATGGGGGTGGTGAAGGGAGGGCCCCAATTGGTAGAAAAAAACCCGCAACCCCTTGGGGTTATCCTGCACTTGGAAGAAGAAGTAGAAAAAGGAATAAATATAGTGATAATTTGATTCTTCGTCGCCGTAGTAAATAG

Protein Analysis

192

Amino Acids

20.44

Weight (kDa)

10.08

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L2_C PF03947 49 - 166 6e-50 Ribosomal Proteins L2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 429
AciI CCGC 1 cut(s) 483
AcoI YGGCCR 2 cut(s) 162, 258
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 443, 509
AgsI TTSAA 1 cut(s) 141
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 3 cut(s) 89, 209, 374
AluI AGCT 3 cut(s) 89, 209, 374
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 3 cut(s) 162, 258, 459
ApaI GGGCCC 1 cut(s) 463
ApeKI GCWGC 1 cut(s) 215
AspS9I GGNCC 3 cut(s) 286, 459, 460
AsuHPI GGTGA 2 cut(s) 355, 461
AvaII GGWCC 1 cut(s) 286
AxyI CCTNAGG 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 463
BbvI GCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 441
BceAI ACGGC 3 cut(s) 55, 177, 549
BclI TGATCA 1 cut(s) 118
BfaI CTAG 2 cut(s) 210, 386
BfmI CTRYAG 3 cut(s) 225, 399, 426
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 1 cut(s) 216
BlpI GCTNAGC 1 cut(s) 310
BlsI GCNGC 1 cut(s) 217
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 286, 459, 460
BmiI GGNNCC 2 cut(s) 461, 462
Bpu1102I GCTNAGC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 439, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 509
Bse21I CCTNAGG 1 cut(s) 154
BseDI CCNNGG 2 cut(s) 439, 491
BseGI GGATG 2 cut(s) 59, 433
BseLI CCNNNNNNNGG 2 cut(s) 443, 509
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 3 cut(s) 164, 260, 461
BsiSI CCGG 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 443, 509
BsnI GGCC 3 cut(s) 164, 260, 461
Bsp120I GGGCCC 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 92
Bsp143I GATC 1 cut(s) 118
Bsp1720I GCTNAGC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 439
BspACI CCGC 1 cut(s) 483
BspANI GGCC 3 cut(s) 164, 260, 461
BspCNI CTCAG 1 cut(s) 323
BspLI GGNNCC 2 cut(s) 461, 462
BspMI ACCTGC 1 cut(s) 209
BsrGI TGTACA 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 439, 491
BssMI GATC 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 439, 491
Bst4CI ACNGT 2 cut(s) 97, 319
BstAPI GCANNNNNTGC 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 154, 310
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 2 cut(s) 59, 433
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 215, 221, 391
BstSFI CTRYAG 3 cut(s) 225, 399, 426
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 1 cut(s) 227
BstXI CCANNNNNNTGG 2 cut(s) 440, 446
Bsu36I CCTNAGG 1 cut(s) 154
BsuRI GGCC 3 cut(s) 164, 260, 461
BtgI CCRYGG 1 cut(s) 439
BtsCI GGATG 2 cut(s) 59, 433
BveI ACCTGC 1 cut(s) 209
CaiI CAGNNNCTG 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 286, 459, 460
CseI GACGC 1 cut(s) 383
Csp6I GTAC 1 cut(s) 93
CviAII CATG 2 cut(s) 41, 440
CviJI RGCY 9 cut(s) 70, 89, 164, 209, 260, 368, 374, 385, 461
CviKI_1 RGCY 9 cut(s) 70, 89, 164, 209, 260, 368, 374, 385, 461
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 2 cut(s) 154, 310
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 162, 258
Eco130I CCWWGG 2 cut(s) 439, 491
Eco24I GRGCYC 1 cut(s) 463
Eco32I GATATC 2 cut(s) 48, 297
Eco47I GGWCC 1 cut(s) 286
Eco81I CCTNAGG 1 cut(s) 154
EcoO109I RGGNCCY 2 cut(s) 459, 460
EcoRV GATATC 2 cut(s) 48, 297
EcoT14I CCWWGG 2 cut(s) 439, 491
EcoT38I GRGCYC 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 439, 491
FaeI CATG 2 cut(s) 44, 443
FatI CATG 2 cut(s) 40, 439
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 46, 420
FriOI GRGCYC 1 cut(s) 463
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 210, 386
GluI GCNGC 1 cut(s) 216
HaeIII GGCC 3 cut(s) 164, 260, 461
HapII CCGG 1 cut(s) 369
HgaI GACGC 1 cut(s) 383
Hin1II CATG 2 cut(s) 44, 443
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 3 cut(s) 23, 131, 553
HpaII CCGG 1 cut(s) 369
HphI GGTGA 2 cut(s) 355, 461
Hpy166II GTNNAC 3 cut(s) 200, 346, 430
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 1 cut(s) 63
Hpy8I GTNNAC 3 cut(s) 200, 346, 430
Hpy99I CGWCG 1 cut(s) 564
HpyAV CCTTC 3 cut(s) 98, 285, 446
HpyCH4III ACNGT 2 cut(s) 97, 319
HpyCH4V TGCA 2 cut(s) 242, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 221, 391
HpyF3I CTNAG 2 cut(s) 154, 310
Hsp92II CATG 2 cut(s) 44, 443
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 8 cut(s) 76, 204, 264, 362, 382, 411, 438, 516
Lsp1109I GCAGC 1 cut(s) 227
MaeI CTAG 2 cut(s) 210, 386
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 5 cut(s) 95, 140, 525, 528, 548
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 463
MluCI AATT 4 cut(s) 203, 269, 465, 547
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 4 cut(s) 241, 277, 401, 450
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 3 cut(s) 41, 438, 444
MspI CCGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 465
MwoI GCNNNNNNNGC 3 cut(s) 215, 221, 391
NcoI CCATGG 1 cut(s) 439
NdeII GATC 1 cut(s) 118
NlaIII CATG 2 cut(s) 44, 443
NlaIV GGNNCC 2 cut(s) 461, 462
OliI CACNNNNGTG 1 cut(s) 41
PaqCI CACCTGC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 23, 131, 553
PkrI GCNGC 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 461, 462
PspOMI GGGCCC 1 cut(s) 459
PspPI GGNCC 3 cut(s) 286, 459, 460
PstNI CAGNNNCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 3 cut(s) 41, 438, 444
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 3 cut(s) 286, 459, 460
SduI GDGCHC 1 cut(s) 463
SetI ASST 7 cut(s) 91, 211, 223, 277, 288, 376, 391
SfcI CTRYAG 3 cut(s) 225, 399, 426
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 3 cut(s) 41, 438, 444
Sse9I AATT 4 cut(s) 203, 269, 465, 547
SsiI CCGC 1 cut(s) 483
SspMI CTAG 2 cut(s) 210, 386
StyI CCWWGG 2 cut(s) 439, 491
TaaI ACNGT 2 cut(s) 97, 319
TasI AATT 4 cut(s) 203, 269, 465, 547
TatI WGTACW 1 cut(s) 92
TfiI GAWTC 3 cut(s) 23, 131, 553
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 434
TspGWI ACGGA 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 2 cut(s) 210, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.