RchiOBHm_Chr2g0118521

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003897
Physical Location & Seq
Forward (+)
30724243 .. 30724671
429 bp
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UTR
Exon/CDS
Intron
PRQ49130

Sequence Viewer

Length: 429 bp
ATGCCCTTAGGCACGGCCATACATAACATAGAAATCACACTTGGAAAGGGTGGACAATTAGCTAGAGCAGCAAGTGCTGTAGCGAAACTGATTGCAAAAGAGGGGAAATCGGCCACATTAAAATTACCTTCTGGGGAGGTCCGTTTGATATCCAAAAACTGCTCAGCAACAATCGGACAAGTGGGGAATGTTGGGGTGAACCAGAAAAGTTTGGGTAAAACCGGAGCTAAATATTGGCTAGGTAAGCTTCCTGTAGTAAGAGGAGTAGTTGTGAACCCTGTAGACCATCCTCATGGGGGTAGTGAAGGGAGGGCCCCAATTGGTAGAAAAAAACCCGCAACCCCTTGGGGTTATCCTGCACTTGGAAGAAGAAGTAGAAAAAGGAATAAATATAGTGATAATTTGATTCTTCGTCGCCGTAGTAAATAG

Protein Analysis

142

Amino Acids

15.08

Weight (kDa)

11.28

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L2_C PF03947 1 - 117 9.3e-46 Ribosomal Proteins L2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 282
AciI CCGC 1 cut(s) 336
AcoI YGGCCR 2 cut(s) 15, 111
AfiI CCNNNNNNNGG 2 cut(s) 296, 362
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 3 cut(s) 62, 227, 247
AluI AGCT 3 cut(s) 62, 227, 247
AoxI GGCC 3 cut(s) 15, 111, 312
ApaI GGGCCC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 139, 312, 313
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 139
AxyI CCTNAGG 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 316
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 294
BceAI ACGGC 2 cut(s) 30, 402
BfaI CTAG 2 cut(s) 63, 239
BfmI CTRYAG 3 cut(s) 78, 252, 279
BisI GCNGC 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 139, 312, 313
BmiI GGNNCC 2 cut(s) 314, 315
Bpu1102I GCTNAGC 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 362
Bse21I CCTNAGG 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 296, 362
BseMII CTCAG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 3 cut(s) 17, 113, 314
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 296, 362
BsnI GGCC 3 cut(s) 17, 113, 314
Bsp120I GGGCCC 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 316
Bsp1720I GCTNAGC 1 cut(s) 163
BspACI CCGC 1 cut(s) 336
BspANI GGCC 3 cut(s) 17, 113, 314
BspCNI CTCAG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 314, 315
BssECI CCNNGG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 74
BstDEI CTNAG 2 cut(s) 7, 163
BstF5I GGATG 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 68, 74, 244
BstSFI CTRYAG 3 cut(s) 78, 252, 279
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 7
BsuRI GGCC 3 cut(s) 17, 113, 314
BtsCI GGATG 1 cut(s) 286
Cfr13I GGNCC 3 cut(s) 139, 312, 313
CviAII CATG 1 cut(s) 293
CviJI RGCY 7 cut(s) 17, 62, 113, 227, 238, 247, 314
CviKI_1 RGCY 7 cut(s) 17, 62, 113, 227, 238, 247, 314
DdeI CTNAG 2 cut(s) 7, 163
EaeI YGGCCR 2 cut(s) 15, 111
Eco130I CCWWGG 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 316
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 7
EcoO109I RGGNCCY 2 cut(s) 312, 313
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 1 cut(s) 296
FaiI YATR 5 cut(s) 20, 24, 29, 294, 393
FatI CATG 1 cut(s) 292
FauI CCCGC 1 cut(s) 343
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 1 cut(s) 273
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 2 cut(s) 63, 239
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 3 cut(s) 17, 113, 314
HapII CCGG 1 cut(s) 222
Hin1II CATG 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 4 cut(s) 53, 199, 274, 283
Hpy188I TCNGA 1 cut(s) 176
Hpy8I GTNNAC 4 cut(s) 53, 199, 274, 283
Hpy99I CGWCG 1 cut(s) 417
HpyAV CCTTC 2 cut(s) 138, 299
HpyCH4V TGCA 2 cut(s) 95, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 74, 244
HpyF3I CTNAG 2 cut(s) 7, 163
Hsp92II CATG 1 cut(s) 296
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 6 cut(s) 117, 215, 235, 264, 291, 369
Lsp1109I GCAGC 1 cut(s) 80
MaeI CTAG 2 cut(s) 63, 239
MboII GAAGA 3 cut(s) 378, 381, 401
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 4 cut(s) 56, 122, 318, 400
MnlI CCTC 5 cut(s) 94, 130, 254, 300, 303
MseI TTAA 1 cut(s) 119
MslI CAYNNNNRTG 1 cut(s) 291
MspI CCGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 318
MwoI GCNNNNNNNGC 3 cut(s) 68, 74, 244
NlaIII CATG 1 cut(s) 296
NlaIV GGNNCC 2 cut(s) 314, 315
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 314, 315
PspOMI GGGCCC 1 cut(s) 312
PspPI GGNCC 3 cut(s) 139, 312, 313
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 1 cut(s) 69
Sau96I GGNCC 3 cut(s) 139, 312, 313
SduI GDGCHC 1 cut(s) 316
SetI ASST 6 cut(s) 64, 130, 141, 229, 244, 249
SfcI CTRYAG 3 cut(s) 78, 252, 279
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 291
Sse9I AATT 4 cut(s) 56, 122, 318, 400
SsiI CCGC 1 cut(s) 336
SspI AATATT 1 cut(s) 233
SspMI CTAG 2 cut(s) 63, 239
StyI CCWWGG 1 cut(s) 344
TasI AATT 4 cut(s) 56, 122, 318, 400
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TseI GCWGC 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 2 cut(s) 63, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.