MD00G1001800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012472
Physical Location & Seq
Forward (+)
387578 .. 391499
3922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1001800.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGTTTGTTAAAACTCCAAAAAAAAAAAAAATCAATAATTCCTCTTCTTCCTTGTGTTCAGTGCTCTTGGACTGGGGTTTCCATTCACCGGATAAATGGTTCCGGTGGCTGGACCTTTTGCAGAAAACCCCACGAATGCCCTTTAATTTACGCCAAGTACCAAGCTTAATGGATTCTGAGGAGGTCTCTGCGAAGGGCAGCGTGGCCAATTTAATCCTCCGGTGGTCTCTGGGTCTGCTTCTCCTTATCGTTGCTTTCTTTTCACTATCGCTGTTGGTCGCCTTCGTCGCCGTTTTCGTCGCCAATTCTTCAATTCCGAGCTCAATTTCAGTGTCCTCTCAGTGCAGGATCGTCTCAAGCAGTGTTGATCTTAAGTCTTCTAAAGTGTGTGAGCTTGGACTGTTCAATTATAAAGCCAAGCATGTGTTTTATCCTTTTCAAGGAAGAAGGTTTCGATGCCGTTATGATTACTACTGGGCTTCAATTTTTAAGGTGGAATACAAAGATCAGTCTTCCGGTCAGACTCAGCTTGCATTGGCTGAGGCTCCAAATGAGGCCCTTCCTCTTAATTGCAGACCTAATTTTGGTGCTGCATGGTTGACCAAAGATAAATTCAAGGTCAATGAAACTTATGACTGCTGGTACACGTATGGCATTTCCAAAGTTAGCTTATATCATGATGGTTTCTTCAGTTGCCAAGCTAAAGATCCGTCTACATTTGAGATGATTACAAGATATCGCATCCTGGCGACAAAGATTTTACACTCCTGGATTGTCAGCCAGAAACGAGGTGGGTTATGGAGGTGGGAAACAATAGCTGGAGTTATTAGTGGGTTTTTAACTTCTTTTATCTCCATAATCTTTGTTAGACTCCTGCAGCAAATGAAATTTAGGTTGCCTCAGATTTTAGCCGCAAGAGTGCTTACTACCGGCTTCAGGCGCACTTGTTTACTTGTGGCATACATTTCTTTTGCGGGTTGGCTGGCTATCCAGTATGGGAAAAGGCTTGGCCTCCCGGAGATAATCGTACTTTTTAAGAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

349

Amino Acids

39.8

Weight (kDa)

9.83

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19140
fragaria_vesca FvH4_5g23960
malus_domestica MD00G1001800.v1.1 MD06G1015300.v1.1
prunus_persica Prupe.5G020400_v2.0.a1
pyrus_communis pycom16g25480
rosa_chinensis RchiOBHm_Chr7g0213391
rosa_laevigata RLG00000002809
rosa_multiflora Rmu_co8237685.1_g000001 Rmu_sc0029136.1_g000001 Rmu_ssc0000262.1_g000033
rosa_roxburghii Rroxscaffold_3G00246030
rosa_rugosa Rorug07G0142100
rosa_samantha Rh7AG273700 Rh7BG269000 Rh7BG269100 Rh7CG292800 Rh7DG281100
rosa_wichuraiana Rw7G023730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AccI GTMKAC 1 cut(s) 713
AciI CCGC 2 cut(s) 912, 974
AclWI GGATC 2 cut(s) 356, 701
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 611, 887
AcuI CTGAAG 2 cut(s) 673, 919
AfaI GTAC 3 cut(s) 159, 644, 1029
AfiI CCNNNNNNNGG 5 cut(s) 88, 109, 440, 584, 936
AflII CTTAAG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 5 cut(s) 312, 406, 440, 483, 616
AjnI CCWGG 2 cut(s) 744, 767
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
AluBI AGCT 7 cut(s) 165, 321, 394, 529, 669, 701, 818
AluI AGCT 7 cut(s) 165, 321, 394, 529, 669, 701, 818
Alw21I GWGCWC 2 cut(s) 66, 323
Alw26I GTCTC 3 cut(s) 190, 231, 358
AlwI GGATC 2 cut(s) 356, 701
AoxI GGCC 3 cut(s) 204, 555, 1009
ApeKI GCWGC 3 cut(s) 198, 590, 877
ApoI RAATTY 2 cut(s) 611, 887
AspLEI GCGC 1 cut(s) 942
AspS9I GGNCC 2 cut(s) 112, 556
AsuC2I CCSGG 1 cut(s) 1016
AsuHPI GGTGA 1 cut(s) 78
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 206
BanII GRGCYC 1 cut(s) 323
BbsI GAAGAC 2 cut(s) 369, 504
Bbv12I GWGCWC 2 cut(s) 66, 323
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 3 cut(s) 210, 577, 889
BccI CCATC 1 cut(s) 674
BceAI ACGGC 2 cut(s) 275, 444
BciT130I CCWGG 2 cut(s) 746, 769
BcnI CCSGG 1 cut(s) 1016
BcoDI GTCTC 3 cut(s) 190, 231, 358
BfmI CTRYAG 1 cut(s) 875
BfrI CTTAAG 1 cut(s) 371
BisI GCNGC 4 cut(s) 199, 591, 878, 912
BlsI GCNGC 4 cut(s) 200, 592, 879, 913
Bme1390I CCNGG 3 cut(s) 746, 769, 1016
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 112, 556
BmiI GGNNCC 2 cut(s) 101, 546
BmrFI CCNGG 3 cut(s) 746, 769, 1016
BmrI ACTGGG 2 cut(s) 82, 484
BmsI GCATC 2 cut(s) 446, 750
BmuI ACTGGG 2 cut(s) 82, 484
BpiI GAAGAC 2 cut(s) 369, 504
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 340
BpuMI CCSGG 1 cut(s) 1016
BsaAI YACGTR 1 cut(s) 648
BsaI GGTCTC 2 cut(s) 190, 231
BsaWI WCCGGW 4 cut(s) 88, 102, 219, 515
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 109, 440, 584, 936
Bse118I RCCGGY 1 cut(s) 929
Bse1I ACTGG 3 cut(s) 77, 479, 991
BseBI CCWGG 2 cut(s) 746, 769
BseGI GGATG 1 cut(s) 741
BseLI CCNNNNNNNGG 5 cut(s) 88, 109, 440, 584, 936
BseMII CTCAG 5 cut(s) 168, 353, 531, 539, 914
BseNI ACTGG 3 cut(s) 77, 479, 991
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 3 cut(s) 210, 577, 889
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 3 cut(s) 206, 557, 1011
BsiHKAI GWGCWC 2 cut(s) 66, 323
BsiSI CCGG 6 cut(s) 89, 103, 220, 516, 930, 1016
BslI CCNNNNNNNGG 5 cut(s) 88, 109, 440, 584, 936
BsmAI GTCTC 3 cut(s) 190, 231, 358
BsmBI CGTCTC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 3 cut(s) 206, 557, 1011
Bso31I GGTCTC 2 cut(s) 190, 231
Bsp1286I GDGCHC 2 cut(s) 66, 323
Bsp143I GATC 4 cut(s) 348, 367, 505, 706
BspACI CCGC 2 cut(s) 912, 974
BspANI GGCC 3 cut(s) 206, 557, 1011
BspCNI CTCAG 5 cut(s) 169, 352, 532, 538, 913
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 101, 546
BspMAI CTGCAG 1 cut(s) 879
BspPI GGATC 2 cut(s) 356, 701
BspTI CTTAAG 1 cut(s) 371
BspTNI GGTCTC 2 cut(s) 190, 231
BsrFI RCCGGY 1 cut(s) 929
BsrI ACTGG 3 cut(s) 77, 479, 991
BssAI RCCGGY 1 cut(s) 929
BssMI GATC 4 cut(s) 348, 367, 505, 706
Bst2UI CCWGG 2 cut(s) 746, 769
Bst4CI ACNGT 1 cut(s) 402
Bst6I CTCTTC 1 cut(s) 49
BstAFI CTTAAG 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 648
BstC8I GCNNGC 2 cut(s) 531, 984
BstDEI CTNAG 5 cut(s) 177, 339, 525, 540, 900
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 741
BstHHI GCGC 1 cut(s) 942
BstKTI GATC 4 cut(s) 351, 370, 508, 709
BstMAI GTCTC 3 cut(s) 190, 231, 358
BstMBI GATC 4 cut(s) 348, 367, 505, 706
BstMWI GCNNNNNNNGC 2 cut(s) 287, 939
BstNI CCWGG 2 cut(s) 746, 769
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 3 cut(s) 744, 767, 1014
BstSFI CTRYAG 1 cut(s) 875
BstV1I GCAGC 3 cut(s) 210, 577, 889
BstV2I GAAGAC 2 cut(s) 369, 504
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
BsuRI GGCC 3 cut(s) 206, 557, 1011
BtsCI GGATG 1 cut(s) 741
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 4 cut(s) 66, 336, 347, 367
Cac8I GCNNGC 2 cut(s) 531, 984
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 942
Cfr10I RCCGGY 1 cut(s) 929
Cfr13I GGNCC 2 cut(s) 112, 556
Csp6I GTAC 3 cut(s) 158, 643, 1028
CviAII CATG 3 cut(s) 422, 594, 677
CviQI GTAC 3 cut(s) 158, 643, 1028
DdeI CTNAG 5 cut(s) 177, 339, 525, 540, 900
DpnI GATC 4 cut(s) 350, 369, 507, 708
DpnII GATC 4 cut(s) 348, 367, 505, 706
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 323
Eco31I GGTCTC 2 cut(s) 190, 231
Eco32I GATATC 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 321
Eco57I CTGAAG 2 cut(s) 673, 919
EcoICRI GAGCTC 1 cut(s) 321
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 744, 767
EcoRV GATATC 1 cut(s) 737
EcoT38I GRGCYC 1 cut(s) 323
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 3 cut(s) 425, 597, 680
FatI CATG 3 cut(s) 421, 593, 676
FauI CCCGC 1 cut(s) 967
FblI GTMKAC 1 cut(s) 713
Fnu4HI GCNGC 4 cut(s) 199, 591, 878, 912
FokI GGATG 1 cut(s) 728
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 199, 591, 878, 912
GlaI GCGC 1 cut(s) 941
GluI GCNGC 4 cut(s) 199, 591, 878, 912
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 3 cut(s) 206, 557, 1011
HapII CCGG 6 cut(s) 89, 103, 220, 516, 930, 1016
HhaI GCGC 1 cut(s) 942
Hin1II CATG 3 cut(s) 425, 597, 680
Hin6I GCGC 1 cut(s) 940
HinP1I GCGC 1 cut(s) 940
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 3 cut(s) 173, 523, 870
HpaII CCGG 6 cut(s) 89, 103, 220, 516, 930, 1016
HphI GGTGA 1 cut(s) 78
Hpy166II GTNNAC 4 cut(s) 600, 645, 714, 950
Hpy188I TCNGA 4 cut(s) 178, 318, 522, 903
Hpy188III TCNNGA 2 cut(s) 677, 1044
Hpy8I GTNNAC 4 cut(s) 600, 645, 714, 950
Hpy99I CGWCG 2 cut(s) 290, 302
HpyAV CCTTC 4 cut(s) 187, 292, 441, 569
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4IV ACGT 1 cut(s) 647
HpyCH4V TGCA 6 cut(s) 121, 345, 533, 573, 593, 877
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 939
HpyF3I CTNAG 5 cut(s) 177, 339, 525, 540, 900
HpySE526I ACGT 1 cut(s) 647
Hsp92II CATG 3 cut(s) 425, 597, 680
HspAI GCGC 1 cut(s) 940
Kzo9I GATC 4 cut(s) 348, 367, 505, 706
LmnI GCTCC 1 cut(s) 550
Lsp1109I GCAGC 3 cut(s) 210, 577, 889
LweI GCATC 2 cut(s) 446, 750
MaeII ACGT 1 cut(s) 647
MalI GATC 4 cut(s) 350, 369, 507, 708
MboI GATC 4 cut(s) 348, 367, 505, 706
MboII GAAGA 7 cut(s) 36, 39, 300, 369, 456, 504, 679
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 2 cut(s) 66, 323
MlsI TGGCCA 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 517, 864
Mox20I TGGCCA 1 cut(s) 206
MscI TGGCCA 1 cut(s) 206
MseI TTAA 9 cut(s) 9, 144, 167, 212, 372, 489, 567, 839, 1035
Msp20I TGGCCA 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 6 cut(s) 89, 103, 220, 516, 930, 1016
MspR9I CCNGG 3 cut(s) 746, 769, 1016
Mva1269I GAATGC 1 cut(s) 141
MvaI CCWGG 2 cut(s) 746, 769
MwoI GCNNNNNNNGC 2 cut(s) 287, 939
NciI CCSGG 1 cut(s) 1016
NdeII GATC 4 cut(s) 348, 367, 505, 706
NlaIII CATG 3 cut(s) 425, 597, 680
NlaIV GGNNCC 2 cut(s) 101, 546
NspI RCATGY 1 cut(s) 425
PagI TCATGA 1 cut(s) 676
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 173
PfoI TCCNGGA 2 cut(s) 767, 1014
PkrI GCNGC 4 cut(s) 200, 592, 879, 913
PleI GAGTC 2 cut(s) 517, 864
PpsI GAGTC 2 cut(s) 517, 864
Ppu21I YACGTR 1 cut(s) 648
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 323
Psp6I CCWGG 2 cut(s) 744, 767
PspGI CCWGG 2 cut(s) 744, 767
PspN4I GGNNCC 2 cut(s) 101, 546
PspPI GGNCC 2 cut(s) 112, 556
PstI CTGCAG 1 cut(s) 879
PsuI RGATCY 1 cut(s) 706
RsaI GTAC 3 cut(s) 159, 644, 1029
RsaNI GTAC 3 cut(s) 158, 643, 1028
SacI GAGCTC 1 cut(s) 323
SaqAI TTAA 9 cut(s) 9, 144, 167, 212, 372, 489, 567, 839, 1035
SatI GCNGC 4 cut(s) 199, 591, 878, 912
Sau3AI GATC 4 cut(s) 348, 367, 505, 706
Sau96I GGNCC 2 cut(s) 112, 556
SchI GAGTC 2 cut(s) 517, 864
ScrFI CCNGG 3 cut(s) 746, 769, 1016
SduI GDGCHC 2 cut(s) 66, 323
SfaNI GCATC 2 cut(s) 446, 750
SfcI CTRYAG 1 cut(s) 875
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 355, 371
SmoI CTYRAG 2 cut(s) 355, 371
SsiI CCGC 2 cut(s) 912, 974
SstI GAGCTC 1 cut(s) 323
StyD4I CCNGG 3 cut(s) 744, 767, 1014
TaaI ACNGT 1 cut(s) 402
TaiI ACGT 1 cut(s) 650
TaqI TCGA 1 cut(s) 454
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 9 cut(s) 9, 144, 167, 212, 372, 489, 567, 839, 1035
Tru9I TTAA 9 cut(s) 9, 144, 167, 212, 372, 489, 567, 839, 1035
TscAI CASTG 4 cut(s) 66, 336, 347, 367
TseI GCWGC 3 cut(s) 198, 590, 877
TspDTI ATGAA 2 cut(s) 639, 899
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 4 cut(s) 66, 336, 347, 367
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 611, 887
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.