Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004890

Physical Location & Seq

Genomic Coordinates Forward (+)

1522267 .. 1525098

Gene Structure 2832 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1012300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGGGGGTCTGCCTGAGCGCCCGAATCAAAGCTGAGAGCTCTGTTAATACAGGTTCAAAATTTGTTAGCAATGATGAGAACCTCAGCAGCACAAGCAGTAAGGTCTCATCATTTTCGGCACCTCCAACTCCTCGGAGCGAGGGCGAGATCTTGAAGTCTCCCAATCTGAAGAGCTTTAGTTATAATGACCTCAAATTGGCCACCAGGAATTTCCGTCCTGATAGTGTGTTGGGAGAAGGTGGATTTGGTTCGGTTTTCAAGGGGTGGATTGATGAAAATTCGTTCACTGCTGCAAAGCCCGGGAGTGGCATAGTTTTGGCTGTGAAACGGCTTAACCAAGAAAGTTTTCAGGGTCACCGAGAGTGGTTGGCAGAAGTGAATTATCTAGGACAGCTCTATCATCCGAATCTCGTGAAACTAATCGGCTACTGCTTGGAGGATGAACACCGACTTCTGGTGTACGAATTCATGCCTCGAGGCAGCTTGGAAAACCATTTGTTCAGGAGAGGCTCTTATTTCCAACCTCTTTCCTGGAACCTCCGGATGAAGGTTGCCCTTGGTGCTGCAAAGGGGCTTGCTTTTCTTCACAGTGCTGAAACAAAAGTGATATACCGAGATTTCAAGACGTCAAACATCCTGATTGATTCGAACTACAATGCAAAACTTTCCGACTTTGGTTTGGCCAAGGACGGGCCAACGGGTGATAAAAGCCACGTCTCCACGAGGGTCATGGGTACCTACGGATACGCAGCTCCAGAATATCTAGCCACAGGTCATCTGACTGCCAAGAGCGACATATATAGCTTCGGAGTCGTTTTGTTGGAGATGTTATCTGGCCGGAGAGCAGTTGACAAGAATCGGCCATCCGGAGAACACAATCTGGTGGAGTGGGCTAAACCCTTCTTGGCAAACAAGCGTAAGATCTTTCGCATCATAGACAACCGTCTGGAAGGCCAGTATTCAATGGACGGAGCTCACAAGGCGGCTACCCTTGCAGCACGATGCATATCCGCAGAATCCAAGCTCAGGCCAAACATGGACGAGGTCGTAACTACACTTGAACAACTGCAAGATTCTAATGCCACCGGAGGCACTCAGAGTAATGGTCAAAGGATGCGCAGACGAAGTGCAGACGATGCTAACAATGCCAAGCGCGCAGCAGATGATGCCTTAAGGGCAGCTAACAAGGCAACGAATACCGTCGCCTATCCTCGGCCATCTGCTTCCCCACTTTACGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0010119 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:1902456 GO:1902458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

414

Amino Acids

45.48

Weight (kDa)

9.43

Isoelectric Point (pI)

33.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	74 - 351	2.7e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	75 - 353	4.9e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004890)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07570 AT1G07570 AT1G07570 AT1G07570 AT2G28930 AT2G28930 AT2G28930
fragaria_vesca	FvH4_3g36200 FvH4_3g36200 FvH4_3g36200 FvH4_3g36200
malus_domestica	MD00G1012300.v1.1 MD03G1092200.v1.1
prunus_persica	Prupe.6G076200_v2.0.a1
pyrus_communis	pycom03g07390 pycom11g08870
rosa_chinensis	RchiOBHm_Chr5g0064971
rosa_laevigata	RLG00000013060 RLG00000022531 RLG00000023667 RLG00000035745
rosa_multiflora	Rmu_sc0003352.1_g000018
rosa_roxburghii	Rroxscaffold_1G00016340
rosa_rugosa	Rorug05G0366800 Rorug05G0366900 Rorug05G0367000
rosa_samantha	Rh5AG425400 Rh5BG440300 Rh5CG462900
rosa_wichuraiana	Rw5G039970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	183
AatII	GACGTC	1 cut(s)	629
AbsI	CCTCGAGG	1 cut(s)	474
Acc16I	TGCGCA	1 cut(s)	1118
Acc65I	GGTACC	1 cut(s)	734
AccB1I	GGYRCC	2 cut(s)	118, 734
AccII	CGCG	1 cut(s)	1155
AccIII	TCCGGA	2 cut(s)	540, 866
AciI	CCGC	2 cut(s)	983, 1011
AcoI	YGGCCR	5 cut(s)	198, 681, 835, 860, 1214
AcsI	RAATTY	4 cut(s)	59, 208, 277, 464
AcuI	CTGAAG	1 cut(s)	188
AcyI	GRCGYC	1 cut(s)	626
AfaI	GTAC	2 cut(s)	461, 736
AfiI	CCNNNNNNNGG	7 cut(s)	196, 305, 454, 547, 690, 1026, 1212
AflII	CTTAAG	1 cut(s)	1171
AgsI	TTSAA	6 cut(s)	57, 154, 259, 622, 963, 1061
AjiI	CACGTC	1 cut(s)	715
AjnI	CCWGG	2 cut(s)	203, 530
AjuI	GAANNNNNNNTTGG	4 cut(s)	228, 260, 330, 362
Alw21I	GWGCWC	2 cut(s)	41, 976
Alw26I	GTCTC	3 cut(s)	109, 162, 721
Ama87I	CYCGRG	2 cut(s)	299, 474
Aor13HI	TCCGGA	2 cut(s)	540, 866
AoxI	GGCC	8 cut(s)	198, 681, 692, 835, 860, 952, 1028, 1214
ApeKI	GCWGC	8 cut(s)	87, 290, 480, 563, 749, 995, 1157, 1178
ApoI	RAATTY	4 cut(s)	59, 208, 277, 464
Asp700I	GAANNNNTTC	1 cut(s)	345
Asp718I	GGTACC	1 cut(s)	734
AspLEI	GCGC	4 cut(s)	20, 1119, 1155, 1157
AspS9I	GGNCC	1 cut(s)	692
AsuC2I	CCSGG	2 cut(s)	300, 301
AsuHPI	GGTGA	2 cut(s)	347, 713
AsuII	TTCGAA	1 cut(s)	647
AvaI	CYCGRG	2 cut(s)	299, 474
BalI	TGGCCA	2 cut(s)	200, 683
BanI	GGYRCC	2 cut(s)	118, 734
BanII	GRGCYC	2 cut(s)	41, 976
BauI	CACGAG	2 cut(s)	410, 721
Bbv12I	GWGCWC	2 cut(s)	41, 976
BbvCI	CCTCAGC	1 cut(s)	83
BbvI	GCAGC	8 cut(s)	99, 277, 492, 550, 761, 1007, 1169, 1190
BccI	CCATC	2 cut(s)	871, 1225
BceAI	ACGGC	1 cut(s)	344
BciT130I	CCWGG	2 cut(s)	205, 532
BciVI	GTATCC	1 cut(s)	737
BcnI	CCSGG	2 cut(s)	300, 301
BcoDI	GTCTC	3 cut(s)	109, 162, 721
BfaI	CTAG	2 cut(s)	386, 764
BfoI	RGCGCY	1 cut(s)	21
BfrI	CTTAAG	1 cut(s)	1171
BfuI	GTATCC	1 cut(s)	737
BglI	GCCNNNNNGGC	1 cut(s)	1175
BglII	AGATCT	2 cut(s)	147, 921
BisI	GCNGC	9 cut(s)	88, 291, 481, 564, 750, 984, 996, 1158, 1179
BlsI	GCNGC	9 cut(s)	89, 292, 482, 565, 751, 985, 997, 1159, 1180
Bme1390I	CCNGG	4 cut(s)	205, 300, 301, 532
BmeT110I	CYCGRG	2 cut(s)	299, 474
BmgBI	CACGTC	1 cut(s)	715
BmgT120I	GGNCC	1 cut(s)	692
BmiI	GGNNCC	3 cut(s)	120, 536, 736
BmrFI	CCNGG	4 cut(s)	205, 300, 301, 532
BmsI	GCATC	5 cut(s)	939, 992, 1104, 1126, 1156
BoxI	GACNNNNGTC	1 cut(s)	942
BpmI	CTGGAG	1 cut(s)	738
Bpu10I	CCTNAGC	3 cut(s)	14, 83, 1025
Bpu14I	TTCGAA	1 cut(s)	647
BpuMI	CCSGG	2 cut(s)	300, 301
BsaBI	GATNNNNATC	1 cut(s)	1006
BsaHI	GRCGYC	1 cut(s)	626
BsaI	GGTCTC	1 cut(s)	109
BsaJI	CCNNGG	5 cut(s)	131, 299, 556, 684, 1211
BsaWI	WCCGGW	3 cut(s)	540, 866, 1085
Bsc4I	CCNNNNNNNGG	7 cut(s)	196, 305, 454, 547, 690, 1026, 1212
Bse1I	ACTGG	1 cut(s)	955
Bse3DI	GCAATG	1 cut(s)	76
Bse8I	GATNNNNATC	1 cut(s)	1006
BseAI	TCCGGA	2 cut(s)	540, 866
BseBI	CCWGG	2 cut(s)	205, 532
BseDI	CCNNGG	5 cut(s)	131, 299, 556, 684, 1211
BseGI	GGATG	6 cut(s)	400, 445, 549, 633, 863, 1119
BseJI	GATNNNNATC	1 cut(s)	1006
BseLI	CCNNNNNNNGG	7 cut(s)	196, 305, 454, 547, 690, 1026, 1212
BseMI	GCAATG	1 cut(s)	76
BseMII	CTCAG	5 cut(s)	5, 24, 97, 1039, 1109
BseNI	ACTGG	1 cut(s)	955
BsePI	GCGCGC	1 cut(s)	1153
BseRI	GAGGAG	1 cut(s)	120
BseXI	GCAGC	8 cut(s)	99, 277, 492, 550, 761, 1007, 1169, 1190
BsgI	GTGCAG	1 cut(s)	1149
Bsh1236I	CGCG	1 cut(s)	1155
BshFI	GGCC	8 cut(s)	200, 683, 694, 837, 862, 954, 1030, 1216
BshNI	GGYRCC	2 cut(s)	118, 734
BsiHKAI	GWGCWC	2 cut(s)	41, 976
BsiHKCI	CYCGRG	2 cut(s)	299, 474
BsiSI	CCGG	5 cut(s)	300, 541, 838, 867, 1086
BslI	CCNNNNNNNGG	7 cut(s)	196, 305, 454, 547, 690, 1026, 1212
BsmAI	GTCTC	3 cut(s)	109, 162, 721
BsmBI	CGTCTC	1 cut(s)	721
BsnI	GGCC	8 cut(s)	200, 683, 694, 837, 862, 954, 1030, 1216
Bso31I	GGTCTC	1 cut(s)	109
BsoBI	CYCGRG	2 cut(s)	299, 474
Bsp119I	TTCGAA	1 cut(s)	647
Bsp1286I	GDGCHC	2 cut(s)	41, 976
Bsp13I	TCCGGA	2 cut(s)	540, 866
Bsp143I	GATC	2 cut(s)	147, 921
BspACI	CCGC	2 cut(s)	983, 1011
BspANI	GGCC	8 cut(s)	200, 683, 694, 837, 862, 954, 1030, 1216
BspCNI	CTCAG	5 cut(s)	6, 25, 96, 1038, 1108
BspEI	TCCGGA	2 cut(s)	540, 866
BspFNI	CGCG	1 cut(s)	1155
BspLI	GGNNCC	3 cut(s)	120, 536, 736
BspQI	GCTCTTC	1 cut(s)	164
BspT104I	TTCGAA	1 cut(s)	647
BspT107I	GGYRCC	2 cut(s)	118, 734
BspTI	CTTAAG	1 cut(s)	1171
BspTNI	GGTCTC	1 cut(s)	109
BsrDI	GCAATG	1 cut(s)	76
BsrI	ACTGG	1 cut(s)	955
BssECI	CCNNGG	5 cut(s)	131, 299, 556, 684, 1211
BssHII	GCGCGC	1 cut(s)	1153
BssMI	GATC	2 cut(s)	147, 921
BssNI	GRCGYC	1 cut(s)	626
BssSI	CACGAG	2 cut(s)	410, 721
BssT1I	CCWWGG	2 cut(s)	556, 684
Bst2BI	CACGAG	2 cut(s)	410, 721
Bst2UI	CCWGG	2 cut(s)	205, 532
Bst4CI	ACNGT	3 cut(s)	590, 944, 1201
Bst6I	CTCTTC	1 cut(s)	164
BstACI	GRCGYC	1 cut(s)	626
BstAFI	CTTAAG	1 cut(s)	1171
BstAPI	GCANNNNNTGC	2 cut(s)	1136, 1166
BstBI	TTCGAA	1 cut(s)	647
BstC8I	GCNNGC	2 cut(s)	576, 1155
BstDEI	CTNAG	5 cut(s)	14, 33, 83, 1025, 1095
BstEII	GGTNACC	1 cut(s)	353
BstF5I	GGATG	6 cut(s)	400, 445, 549, 633, 863, 1119
BstFNI	CGCG	1 cut(s)	1155
BstH2I	RGCGCY	1 cut(s)	21
BstHHI	GCGC	4 cut(s)	20, 1119, 1155, 1157
BstKTI	GATC	2 cut(s)	150, 924
BstMAI	GTCTC	3 cut(s)	109, 162, 721
BstMBI	GATC	2 cut(s)	147, 921
BstNI	CCWGG	2 cut(s)	205, 532
BstPAI	GACNNNNGTC	1 cut(s)	942
BstPI	GGTNACC	1 cut(s)	353
BstSCI	CCNGG	4 cut(s)	203, 298, 299, 530
BstUI	CGCG	1 cut(s)	1155
BstV1I	GCAGC	8 cut(s)	99, 277, 492, 550, 761, 1007, 1169, 1190
BstX2I	RGATCY	2 cut(s)	147, 921
BstYI	RGATCY	2 cut(s)	147, 921
BsuI	GTATCC	1 cut(s)	737
BsuRI	GGCC	8 cut(s)	200, 683, 694, 837, 862, 954, 1030, 1216
BtrI	CACGTC	1 cut(s)	715
BtsCI	GGATG	6 cut(s)	400, 445, 549, 633, 863, 1119
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	2 cut(s)	285, 595
Cac8I	GCNNGC	2 cut(s)	576, 1155
CfoI	GCGC	4 cut(s)	20, 1119, 1155, 1157
Cfr13I	GGNCC	1 cut(s)	692
Cfr9I	CCCGGG	1 cut(s)	299
Csp6I	GTAC	2 cut(s)	460, 735
CviAII	CATG	3 cut(s)	469, 730, 1036
CviQI	GTAC	2 cut(s)	460, 735
DdeI	CTNAG	5 cut(s)	14, 33, 83, 1025, 1095
DpnI	GATC	2 cut(s)	149, 923
DpnII	GATC	2 cut(s)	147, 921
EaeI	YGGCCR	5 cut(s)	198, 681, 835, 860, 1214
Eam1104I	CTCTTC	1 cut(s)	164
EarI	CTCTTC	1 cut(s)	164
Ecl136II	GAGCTC	2 cut(s)	39, 974
Eco130I	CCWWGG	2 cut(s)	556, 684
Eco24I	GRGCYC	2 cut(s)	41, 976
Eco31I	GGTCTC	1 cut(s)	109
Eco53kI	GAGCTC	2 cut(s)	39, 974
Eco57I	CTGAAG	1 cut(s)	188
Eco88I	CYCGRG	2 cut(s)	299, 474
Eco91I	GGTNACC	1 cut(s)	353
EcoICRI	GAGCTC	2 cut(s)	39, 974
EcoO65I	GGTNACC	1 cut(s)	353
EcoRI	GAATTC	1 cut(s)	464
EcoRII	CCWGG	2 cut(s)	203, 530
EcoT14I	CCWWGG	2 cut(s)	556, 684
EcoT22I	ATGCAT	1 cut(s)	1007
EcoT38I	GRGCYC	2 cut(s)	41, 976
ErhI	CCWWGG	2 cut(s)	556, 684
Esp3I	CGTCTC	1 cut(s)	721
FaeI	CATG	3 cut(s)	472, 733, 1039
FatI	CATG	3 cut(s)	468, 729, 1035
Fnu4HI	GCNGC	9 cut(s)	88, 291, 481, 564, 750, 984, 996, 1158, 1179
FokI	GGATG	6 cut(s)	387, 452, 556, 620, 850, 1126
FriOI	GRGCYC	2 cut(s)	41, 976
Fsp4HI	GCNGC	9 cut(s)	88, 291, 481, 564, 750, 984, 996, 1158, 1179
FspBI	CTAG	2 cut(s)	386, 764
FspI	TGCGCA	1 cut(s)	1118
GlaI	GCGC	4 cut(s)	19, 1118, 1154, 1156
GluI	GCNGC	9 cut(s)	88, 291, 481, 564, 750, 984, 996, 1158, 1179
GsuI	CTGGAG	1 cut(s)	738
HaeII	RGCGCY	1 cut(s)	21
HaeIII	GGCC	8 cut(s)	200, 683, 694, 837, 862, 954, 1030, 1216
HapII	CCGG	5 cut(s)	300, 541, 838, 867, 1086
HhaI	GCGC	4 cut(s)	20, 1119, 1155, 1157
Hin1I	GRCGYC	1 cut(s)	626
Hin1II	CATG	3 cut(s)	472, 733, 1039
Hin6I	GCGC	4 cut(s)	18, 1117, 1153, 1155
HinP1I	GCGC	4 cut(s)	18, 1117, 1153, 1155
HincII	GTYRAC	1 cut(s)	850
HindII	GTYRAC	1 cut(s)	850
HinfI	GANTC	7 cut(s)	24, 406, 644, 810, 856, 1016, 1073
HpaII	CCGG	5 cut(s)	300, 541, 838, 867, 1086
HphI	GGTGA	2 cut(s)	347, 713
Hpy166II	GTNNAC	3 cut(s)	285, 460, 850
Hpy188I	TCNGA	7 cut(s)	135, 168, 405, 670, 780, 809, 1098
Hpy8I	GTNNAC	3 cut(s)	285, 460, 850
Hpy99I	CGWCG	1 cut(s)	1205
HpyAV	CCTTC	4 cut(s)	230, 541, 910, 944
HpyCH4III	ACNGT	3 cut(s)	590, 944, 1201
HpyCH4IV	ACGT	2 cut(s)	626, 714
HpyCH4V	TGCA	7 cut(s)	293, 566, 659, 995, 1005, 1069, 1130
HpyF3I	CTNAG	5 cut(s)	14, 33, 83, 1025, 1095
HpySE526I	ACGT	2 cut(s)	626, 714
Hsp92I	GRCGYC	1 cut(s)	626
Hsp92II	CATG	3 cut(s)	472, 733, 1039
HspAI	GCGC	4 cut(s)	18, 1117, 1153, 1155
Kpn2I	TCCGGA	2 cut(s)	540, 866
KpnI	GGTACC	1 cut(s)	738
Kzo9I	GATC	2 cut(s)	147, 921
LguI	GCTCTTC	1 cut(s)	164
LmnI	GCTCC	3 cut(s)	135, 757, 971
Lsp1109I	GCAGC	8 cut(s)	99, 277, 492, 550, 761, 1007, 1169, 1190
LweI	GCATC	5 cut(s)	939, 992, 1104, 1126, 1156
MaeI	CTAG	2 cut(s)	386, 764
MaeII	ACGT	2 cut(s)	626, 714
MaeIII	GTNAC	2 cut(s)	353, 1048
MalI	GATC	2 cut(s)	149, 923
MboI	GATC	2 cut(s)	147, 921
MboII	GAAGA	2 cut(s)	181, 575
MflI	RGATCY	2 cut(s)	147, 921
MhlI	GDGCHC	2 cut(s)	41, 976
MlsI	TGGCCA	2 cut(s)	200, 683
MluCI	AATT	6 cut(s)	59, 194, 208, 277, 379, 464
MluNI	TGGCCA	2 cut(s)	200, 683
MlyI	GAGTC	1 cut(s)	819
MmeI	TCCRAC	4 cut(s)	149, 544, 693, 801
Mox20I	TGGCCA	2 cut(s)	200, 683
Mph1103I	ATGCAT	1 cut(s)	1007
MroI	TCCGGA	2 cut(s)	540, 866
MroXI	GAANNNNTTC	1 cut(s)	345
MscI	TGGCCA	2 cut(s)	200, 683
MseI	TTAA	3 cut(s)	45, 333, 1172
Msp20I	TGGCCA	2 cut(s)	200, 683
MspCI	CTTAAG	1 cut(s)	1171
MspI	CCGG	5 cut(s)	300, 541, 838, 867, 1086
MspR9I	CCNGG	4 cut(s)	205, 300, 301, 532
MvaI	CCWGG	2 cut(s)	205, 532
MvnI	CGCG	1 cut(s)	1155
NciI	CCSGG	2 cut(s)	300, 301
NdeII	GATC	2 cut(s)	147, 921
NlaIII	CATG	3 cut(s)	472, 733, 1039
NlaIV	GGNNCC	3 cut(s)	120, 536, 736
NmeAIII	GCCGAG	1 cut(s)	1192
NmuCI	GTSAC	1 cut(s)	353
NsbI	TGCGCA	1 cut(s)	1118
NsiI	ATGCAT	1 cut(s)	1007
NspV	TTCGAA	1 cut(s)	647
PaeR7I	CTCGAG	1 cut(s)	474
PauI	GCGCGC	1 cut(s)	1153
PciSI	GCTCTTC	1 cut(s)	164
PdmI	GAANNNNTTC	1 cut(s)	345
PfeI	GAWTC	6 cut(s)	24, 406, 644, 856, 1016, 1073
PflFI	GACNNNGTC	1 cut(s)	1043
PfoI	TCCNGGA	1 cut(s)	530
PkrI	GCNGC	9 cut(s)	89, 292, 482, 565, 751, 985, 997, 1159, 1180
PleI	GAGTC	1 cut(s)	818
PpsI	GAGTC	1 cut(s)	818
PshAI	GACNNNNGTC	1 cut(s)	942
PsiI	TTATAA	1 cut(s)	183
Psp124BI	GAGCTC	2 cut(s)	41, 976
Psp6I	CCWGG	2 cut(s)	203, 530
PspEI	GGTNACC	1 cut(s)	353
PspGI	CCWGG	2 cut(s)	203, 530
PspN4I	GGNNCC	3 cut(s)	120, 536, 736
PspPI	GGNCC	1 cut(s)	692
PspXI	VCTCGAGB	1 cut(s)	474
PsuI	RGATCY	2 cut(s)	147, 921
PsyI	GACNNNGTC	1 cut(s)	1043
PteI	GCGCGC	1 cut(s)	1153
RsaI	GTAC	2 cut(s)	461, 736
RsaNI	GTAC	2 cut(s)	460, 735
SacI	GAGCTC	2 cut(s)	41, 976
SapI	GCTCTTC	1 cut(s)	164
SaqAI	TTAA	3 cut(s)	45, 333, 1172
SatI	GCNGC	9 cut(s)	88, 291, 481, 564, 750, 984, 996, 1158, 1179
Sau3AI	GATC	2 cut(s)	147, 921
Sau96I	GGNCC	1 cut(s)	692
SchI	GAGTC	1 cut(s)	819
ScrFI	CCNGG	4 cut(s)	205, 300, 301, 532
SduI	GDGCHC	2 cut(s)	41, 976
SfaNI	GCATC	5 cut(s)	939, 992, 1104, 1126, 1156
Sfr274I	CTCGAG	1 cut(s)	474
SfuI	TTCGAA	1 cut(s)	647
SlaI	CTCGAG	1 cut(s)	474
SmaI	CCCGGG	1 cut(s)	301
SmlI	CTYRAG	2 cut(s)	474, 1171
SmoI	CTYRAG	2 cut(s)	474, 1171
Sse9I	AATT	6 cut(s)	59, 194, 208, 277, 379, 464
SsiI	CCGC	2 cut(s)	983, 1011
SspMI	CTAG	2 cut(s)	386, 764
SstI	GAGCTC	2 cut(s)	41, 976
StyD4I	CCNGG	4 cut(s)	203, 298, 299, 530
StyI	CCWWGG	2 cut(s)	556, 684
TaaI	ACNGT	3 cut(s)	590, 944, 1201
TaiI	ACGT	2 cut(s)	629, 717
TaqI	TCGA	2 cut(s)	475, 647
TasI	AATT	6 cut(s)	59, 194, 208, 277, 379, 464
TauI	GCSGC	1 cut(s)	986
TfiI	GAWTC	6 cut(s)	24, 406, 644, 856, 1016, 1073
Tru1I	TTAA	3 cut(s)	45, 333, 1172
Tru9I	TTAA	3 cut(s)	45, 333, 1172
TscAI	CASTG	2 cut(s)	292, 595
TseFI	GTSAC	1 cut(s)	353
TseI	GCWGC	8 cut(s)	87, 290, 480, 563, 749, 995, 1157, 1178
Tsp45I	GTSAC	1 cut(s)	353
TspDTI	ATGAA	4 cut(s)	288, 456, 457, 560
TspGWI	ACGGA	3 cut(s)	203, 756, 984
TspMI	CCCGGG	1 cut(s)	299
TspRI	CASTG	2 cut(s)	292, 595
Tth111I	GACNNNGTC	1 cut(s)	1043
Vha464I	CTTAAG	1 cut(s)	1171
XapI	RAATTY	4 cut(s)	59, 208, 277, 464
XcmI	CCANNNNNNNNNTGG	1 cut(s)	727
XhoI	CTCGAG	1 cut(s)	474
XmaI	CCCGGG	1 cut(s)	299
XmnI	GAANNNNTTC	1 cut(s)	345
XspI	CTAG	2 cut(s)	386, 764
ZraI	GACGTC	1 cut(s)	627
Zsp2I	ATGCAT	1 cut(s)	1007

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.