MD00G1060200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010526
Physical Location & Seq
Reverse (-)
11186851 .. 11188170
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1060200.v1.1.491

Sequence Viewer

Length: 444 bp
ATGCACCTAAGTTACCCAGACAATCAATTTCTTCATCCAAAGAACAGCAAAAATCTTGGCAAGGCTACAACACCAGATGCTAAAGCTTGCTTAGTCCTCCGTCCTCGCGCCGTGTTATCGAGTCCTGAAAATGATGGGATGATTGGAAGCATAAACAAGATAGTCGATCGTAAGAACTCACCGGCTATCAAAGTGCAAAACCCTAATACTGCTACCAGTTCTGCACAAAGGCCCCATCAAGAAGCCAAGAAAGCTCCAAGTCAGGCGAAGAAGAAGGCAACTGCAACTGCAAGGCCTATGGCTATGAACACGAACAAAGGCTCCAATATTAAAACATTATTTAACAGTAATAATGGCGATGTGAAAAGTAAGTTTCAGAAAGCCTTGGTGGGAAAACAGCATGCATGTCTTGGAACGTGGAAACCAAGTTTCACTTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

15.88

Weight (kDa)

10.36

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 108
AgsI TTSAA 1 cut(s) 438
AjuI GAANNNNNNNTTGG 1 cut(s) 418
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 2 cut(s) 86, 254
AluI AGCT 2 cut(s) 86, 254
AoxI GGCC 2 cut(s) 230, 293
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 171
BccI CCATC 2 cut(s) 128, 243
BceAI ACGGC 1 cut(s) 95
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 233, 322
BmsI GCATC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 384
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 34, 144
BseNI ACTGG 1 cut(s) 216
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 232, 295
BsiEI CGRYCG 1 cut(s) 169
BsiSI CCGG 1 cut(s) 182
BsnI GGCC 2 cut(s) 232, 295
Bsp143I GATC 1 cut(s) 166
BspANI GGCC 2 cut(s) 232, 295
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 233, 322
BsrFI RCCGGY 1 cut(s) 181
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 88, 402
BstDEI CTNAG 2 cut(s) 8, 91
BstF5I GGATG 2 cut(s) 34, 144
BstFNI CGCG 1 cut(s) 108
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNSI RCATGY 2 cut(s) 404, 408
BstUI CGCG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 232, 295
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 34, 144
Cac8I GCNNGC 2 cut(s) 88, 402
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 231
CviAII CATG 2 cut(s) 401, 405
DdeI CTNAG 2 cut(s) 8, 91
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 384
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 404, 408
FaiI YATR 5 cut(s) 152, 299, 305, 402, 406
FalI AAGNNNNNCTT 1 cut(s) 418
FatI CATG 2 cut(s) 400, 404
FokI GGATG 2 cut(s) 21, 151
GlaI GCGC 1 cut(s) 109
HaeIII GGCC 2 cut(s) 232, 295
HapII CCGG 1 cut(s) 182
HhaI GCGC 1 cut(s) 110
Hin1II CATG 2 cut(s) 404, 408
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 1 cut(s) 121
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 2 cut(s) 125, 239
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 6 cut(s) 4, 196, 224, 284, 290, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 8, 91
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 2 cut(s) 404, 408
HspAI GCGC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 2 cut(s) 259, 326
LpnPI CCDG 6 cut(s) 30, 87, 138, 195, 229, 248
LweI GCATC 1 cut(s) 67
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 11
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 3 cut(s) 23, 280, 283
MluCI AATT 1 cut(s) 26
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 2 cut(s) 107, 114
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 330, 342, 442
MspI CCGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 251
NdeII GATC 1 cut(s) 166
NlaIII CATG 2 cut(s) 404, 408
NlaIV GGNNCC 2 cut(s) 233, 322
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 2 cut(s) 404, 408
PaeI GCATGC 1 cut(s) 404
PceI AGGCCT 1 cut(s) 295
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 233, 322
PspPI GGNCC 1 cut(s) 231
PvuI CGATCG 1 cut(s) 169
SaqAI TTAA 3 cut(s) 330, 342, 442
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 130
SetI ASST 4 cut(s) 9, 88, 256, 419
SfaNI GCATC 1 cut(s) 67
SphI GCATGC 1 cut(s) 404
Sse9I AATT 1 cut(s) 26
SseBI AGGCCT 1 cut(s) 295
SspI AATATT 1 cut(s) 328
StuI AGGCCT 1 cut(s) 295
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 1 cut(s) 419
TaqI TCGA 2 cut(s) 119, 165
TasI AATT 1 cut(s) 26
Tru1I TTAA 3 cut(s) 330, 342, 442
Tru9I TTAA 3 cut(s) 330, 342, 442
TspDTI ATGAA 2 cut(s) 23, 320
TspGWI ACGGA 1 cut(s) 89
XceI RCATGY 2 cut(s) 404, 408
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.