Rorug06G0182500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010526
Physical Location & Seq
Forward (+)
28853530 .. 28854513
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0182500.1

Sequence Viewer

Length: 360 bp
ATGACGGTAAAGCTGACGCGCAGCGCGGTAGACACCATCATCGGCGGCAATGTCTACTTGAAGCCGGTGATGTTGGTCACCGATATCAGTCCCATCAGGCCCAACAACATGCCAGTGGGCTATCAGTTTATGGTCTCCGACGTCGTTTGTTCTCATCGGGCCATGGCCGCATTTCAGCTCAACGACCTATTTGGGACTGACCAGGTCAAGAAAGGCTCCGTCGTCCGGCTCACCGAGTACATTTGCTCCAAAGTCGAGGATCGCAAGCTTATTGTTGTGATGAATATGGAAACTATTGTACTAGACTCGGGGCTTATTGGGAATCCAAAAGAATATGTTCAGTCTGGTACAACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.08

Weight (kDa)

7.75

Isoelectric Point (pI)

19.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 100 6.3e-20 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
AccI GTMKAC 2 cut(s) 30, 54
AccII CGCG 2 cut(s) 19, 26
AciI CCGC 3 cut(s) 26, 45, 168
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 165
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 3 cut(s) 239, 300, 349
AfiI CCNNNNNNNGG 1 cut(s) 225
AgsI TTSAA 1 cut(s) 61
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 3 cut(s) 13, 178, 268
AluI AGCT 3 cut(s) 13, 178, 268
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 3 cut(s) 98, 159, 165
ApeKI GCWGC 1 cut(s) 21
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 2 cut(s) 21, 26
AspS9I GGNCC 2 cut(s) 99, 159
AsuHPI GGTGA 3 cut(s) 70, 79, 223
AvaI CYCGRG 1 cut(s) 307
BbvI GCAGC 1 cut(s) 33
BccI CCATC 2 cut(s) 44, 101
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 302
BisI GCNGC 3 cut(s) 22, 46, 168
BlsI GCNGC 3 cut(s) 23, 47, 169
Bme1390I CCNGG 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 99, 159
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 55
BseNI ACTGG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 2 cut(s) 19, 26
BshFI GGCC 3 cut(s) 100, 161, 167
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 2 cut(s) 65, 226
BslFI GGGAC 2 cut(s) 75, 208
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 75, 208
BsnI GGCC 3 cut(s) 100, 161, 167
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 307
Bsp143I GATC 1 cut(s) 259
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 3 cut(s) 26, 45, 168
BspANI GGCC 3 cut(s) 100, 161, 167
BspFNI CGCG 2 cut(s) 19, 26
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 259
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 7
BstACI GRCGYC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 162
BstEII GGTNACC 1 cut(s) 76
BstFNI CGCG 2 cut(s) 19, 26
BstHHI GCGC 2 cut(s) 21, 26
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 112
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 201
BstUI CGCG 2 cut(s) 19, 26
BstV1I GCAGC 1 cut(s) 33
BsuRI GGCC 3 cut(s) 100, 161, 167
BtgI CCRYGG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 2 cut(s) 21, 26
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 99, 159
CseI GACGC 1 cut(s) 25
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 3 cut(s) 238, 299, 348
CviAII CATG 2 cut(s) 109, 163
CviQI GTAC 3 cut(s) 238, 299, 348
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 307
Eco91I GGTNACC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 112, 166
FaiI YATR 5 cut(s) 110, 131, 164, 287, 336
FaqI GGGAC 2 cut(s) 75, 208
FatI CATG 2 cut(s) 108, 162
FblI GTMKAC 2 cut(s) 30, 54
Fnu4HI GCNGC 3 cut(s) 22, 46, 168
Fsp4HI GCNGC 3 cut(s) 22, 46, 168
FspBI CTAG 1 cut(s) 302
GlaI GCGC 2 cut(s) 20, 25
GluI GCNGC 3 cut(s) 22, 46, 168
HaeIII GGCC 3 cut(s) 100, 161, 167
HapII CCGG 2 cut(s) 65, 226
HgaI GACGC 1 cut(s) 25
HhaI GCGC 2 cut(s) 21, 26
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 2 cut(s) 112, 166
Hin6I GCGC 2 cut(s) 19, 24
HinP1I GCGC 2 cut(s) 19, 24
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 2 cut(s) 305, 322
HpaII CCGG 2 cut(s) 65, 226
HphI GGTGA 3 cut(s) 70, 79, 223
Hpy166II GTNNAC 2 cut(s) 31, 55
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 2 cut(s) 31, 55
Hpy99I CGWCG 3 cut(s) 143, 146, 224
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 2 cut(s) 112, 166
HspAI GCGC 2 cut(s) 19, 24
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 2 cut(s) 221, 251
LpnPI CCDG 7 cut(s) 78, 82, 126, 188, 215, 239, 330
Lsp1109I GCAGC 1 cut(s) 33
MabI ACCWGGT 1 cut(s) 201
MaeI CTAG 1 cut(s) 302
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 1 cut(s) 76
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 1 cut(s) 358
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 2 cut(s) 65, 226
MspR9I CCNGG 1 cut(s) 203
MteI GCGCNGCGC 1 cut(s) 22
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 2 cut(s) 19, 26
MwoI GCNNNNNNNGC 1 cut(s) 167
NcoI CCATGG 1 cut(s) 162
NdeII GATC 1 cut(s) 259
NlaIII CATG 2 cut(s) 112, 166
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 322
PflFI GACNNNGTC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 23, 47, 169
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 2 cut(s) 99, 159
PsyI GACNNNGTC 1 cut(s) 203
RsaI GTAC 3 cut(s) 239, 300, 349
RsaNI GTAC 3 cut(s) 238, 299, 348
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 3 cut(s) 22, 46, 168
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 2 cut(s) 99, 159
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 203
SetI ASST 6 cut(s) 15, 144, 180, 189, 207, 270
SexAI ACCWGGT 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 113
SsiI CCGC 3 cut(s) 26, 45, 168
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 255
TatI WGTACW 2 cut(s) 237, 298
TauI GCSGC 2 cut(s) 48, 170
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 1 cut(s) 120
Tth111I GACNNNGTC 1 cut(s) 203
XceI RCATGY 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 30, 54
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 1 cut(s) 302
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.