Rorug06G0182700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010526
Physical Location & Seq
Forward (+)
28858841 .. 28859774
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0182700.1

Sequence Viewer

Length: 606 bp
ATGGGAAATCGTTTGCTTTCTAACGTTTCACATGGGTTAAATTGCGCGTTTGCTTTTAGCGCGTCATTTCATGATCTGGGCAATGAATTCGAGATAACTCTGGATGAAAGTTCAAAAGTGCAACTGTGCGCTCATGATGACTCAATACCAGACCAGCTATATGACTTCAAGCACATTCGTGCAGTTGAGGATGTTGACGCTAATTCTGTAGTTAATGTTATTGGTATTCTGATATATGTCGGCCCTTCTGTTCCTGGAAAGGCAAACAATGATGTGAGAGCTCAGAGAAGAAATCTGCGTATAAAGGATTTCACTGATAAAAGCGTTGTGATACTGATAACCATATGGGGGGAGGACTATGACAGGCAAGGTCAAAGGCTGAGTGACATGCTCGATTCTAATCATTTTCCAGTTTTAGCTGTCATACGCGGGAGGGTTAATGACTTCAACCAGACCAGATCCATAACAATAAATCGTTCTAGCCGGCTACTCATTGACCCAGATTTTCGCAAGGCTCATCAGTTGAGGGACTGGTTTGTTCGTGAGGGAAAGGCTGCCCGTCATGATGATATTAAAGCCCCAGCTGACACCAACTACCATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.77

Weight (kDa)

6.22

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REPA_OB_2 PF16900 59 - 165 9.1e-14 Replication protein A OB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 47, 62, 429
AciI CCGC 1 cut(s) 429
AclI AACGTT 1 cut(s) 24
AclWI GGATC 1 cut(s) 453
AcsI RAATTY 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 3 cut(s) 114, 169, 448
AjnI CCWGG 1 cut(s) 253
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 4 cut(s) 157, 281, 419, 584
AluI AGCT 4 cut(s) 157, 281, 419, 584
Alw21I GWGCWC 1 cut(s) 283
AlwI GGATC 1 cut(s) 453
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 3 cut(s) 47, 62, 131
AspS9I GGNCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 541
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 255
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
Bme1390I CCNGG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 598
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse118I RCCGGY 1 cut(s) 483
Bse1I ACTGG 2 cut(s) 410, 536
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 2 cut(s) 109, 196
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 296, 371
BseNI ACTGG 2 cut(s) 410, 536
BseXI GCAGC 1 cut(s) 541
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 3 cut(s) 47, 62, 429
BshFI GGCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 484
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 2 cut(s) 73, 458
Bsp19I CCATGG 1 cut(s) 598
BspACI CCGC 1 cut(s) 429
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 295, 372
BspFNI CGCG 3 cut(s) 47, 62, 429
BspHI TCATGA 3 cut(s) 70, 133, 562
BspPI GGATC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 483
BsrI ACTGG 2 cut(s) 410, 536
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 2 cut(s) 73, 458
BssT1I CCWWGG 1 cut(s) 598
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 2 cut(s) 282, 380
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 2 cut(s) 109, 196
BstFNI CGCG 3 cut(s) 47, 62, 429
BstHHI GCGC 3 cut(s) 47, 62, 131
BstKTI GATC 2 cut(s) 76, 461
BstMBI GATC 2 cut(s) 73, 458
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 3 cut(s) 47, 62, 429
BstV1I GCAGC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
BsuRI GGCC 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 598
BtsCI GGATG 2 cut(s) 109, 196
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 485
CciI TCATGA 3 cut(s) 70, 133, 562
CfoI GCGC 3 cut(s) 47, 62, 131
Cfr10I RCCGGY 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 2 cut(s) 51, 206
CviAII CATG 6 cut(s) 32, 71, 134, 388, 563, 599
DdeI CTNAG 2 cut(s) 282, 380
DpnI GATC 2 cut(s) 75, 460
DpnII GATC 2 cut(s) 73, 458
Ecl136II GAGCTC 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 598
FaeI CATG 6 cut(s) 35, 74, 137, 391, 566, 602
FaqI GGGAC 1 cut(s) 542
FatI CATG 6 cut(s) 31, 70, 133, 387, 562, 598
FauI CCCGC 1 cut(s) 422
FauNDI CATATG 1 cut(s) 344
Fnu4HI GCNGC 1 cut(s) 555
FokI GGATG 2 cut(s) 116, 203
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 1 cut(s) 555
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 46, 61, 130
GluI GCNGC 1 cut(s) 555
GsaI CCCAGC 1 cut(s) 584
HaeIII GGCC 1 cut(s) 243
HapII CCGG 1 cut(s) 484
HgaI GACGC 2 cut(s) 51, 206
HhaI GCGC 3 cut(s) 47, 62, 131
Hin1II CATG 6 cut(s) 35, 74, 137, 391, 566, 602
Hin6I GCGC 3 cut(s) 45, 60, 129
HinP1I GCGC 3 cut(s) 45, 60, 129
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 2 cut(s) 140, 395
HpaII CCGG 1 cut(s) 484
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 231, 285
Hpy188III TCNNGA 6 cut(s) 71, 91, 101, 134, 542, 563
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 1 cut(s) 126
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 2 cut(s) 121, 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 282, 380
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 6 cut(s) 35, 74, 137, 391, 566, 602
HspAI GCGC 3 cut(s) 45, 60, 129
KroI GCCGGC 1 cut(s) 483
KroNI GCCGGC 1 cut(s) 485
Kzo9I GATC 2 cut(s) 73, 458
Lsp1109I GCAGC 1 cut(s) 541
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 383
MalI GATC 2 cut(s) 75, 460
MboI GATC 2 cut(s) 73, 458
MboII GAAGA 1 cut(s) 300
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 3 cut(s) 40, 86, 202
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 5 cut(s) 181, 346, 426, 519, 538
MroNI GCCGGC 1 cut(s) 483
MseI TTAA 4 cut(s) 38, 213, 438, 573
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 1 cut(s) 484
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 3 cut(s) 47, 62, 429
MwoI GCNNNNNNNGC 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 485
NcoI CCATGG 1 cut(s) 598
NdeI CATATG 1 cut(s) 344
NdeII GATC 2 cut(s) 73, 458
NgoMIV GCCGGC 1 cut(s) 483
NlaIII CATG 6 cut(s) 35, 74, 137, 391, 566, 602
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 3 cut(s) 70, 133, 562
PdiI GCCGGC 1 cut(s) 485
PfeI GAWTC 1 cut(s) 395
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 1 cut(s) 556
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 1 cut(s) 253
PspPI GGNCC 1 cut(s) 242
PsuI RGATCY 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 584
RseI CAYNNNNRTG 1 cut(s) 177
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 4 cut(s) 38, 213, 438, 573
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 2 cut(s) 73, 458
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 283
SetI ASST 6 cut(s) 27, 159, 283, 373, 421, 586
SfcI CTRYAG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 3 cut(s) 40, 86, 202
SsiI CCGC 1 cut(s) 429
SspMI CTAG 1 cut(s) 480
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 598
TaaI ACNGT 1 cut(s) 126
TaiI ACGT 1 cut(s) 27
TaqI TCGA 2 cut(s) 90, 393
TasI AATT 3 cut(s) 40, 86, 202
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 4 cut(s) 38, 213, 438, 573
Tru9I TTAA 4 cut(s) 38, 213, 438, 573
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 3 cut(s) 59, 99, 120
TspRI CASTG 1 cut(s) 319
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 391
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.