Rorug06G0182600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010526
Physical Location & Seq
Forward (+)
28854526 .. 28855240
715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0182600.1

Sequence Viewer

Length: 534 bp
ATGAACAATTTCCAGCAGTCTGCCTATGGCAACTTTCATAATATTGGCCAAGGACCTATTTCCAAATGGCAGAATCCCAGGCCCGACTCTGTTTCCAGTATGCCAAATGTCAGACGTACATTTGAATCTCAAAGCTCTTCTTATAATGTTCTGAATCCTGGAAGCTCAGCTCCCAGTGTGCCAAATTTCCGAGCTACTATTGGGCAACCTTCCTACCAGCCACCTCCACAGTCTAAAAATAGTGGCCCTGTGTCCGATAATGAGGCGCTACCATATATTACTCCTATTGATGATCTAAAGTGTGGACAGACAGGATGGGTTATCAAAGCAAGAGTCACTGCAAAAGGGGATCAGCGTACATTTAACGATGCTGGACATGCTGGTAAGGTTTTCTCTTTCGACCTCTGTGACCAGGGTGGGCAAATACTAGTGGTCTGCTTTGATGTTGTTGTTGACCAATTTTATGGAGTGATTGAGGTTGGCAAAGTTTATTGGATATCAAAGGGTACTTTGGAGCAAATGGAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.44

Weight (kDa)

7.68

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 106 - 172 1.1e-06 OB-fold nucleic acid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 144
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 184
AfaI GTAC 3 cut(s) 118, 358, 508
AgsI TTSAA 1 cut(s) 125
AhlI ACTAGT 1 cut(s) 427
AjnI CCWGG 3 cut(s) 77, 157, 411
AluBI AGCT 4 cut(s) 135, 165, 170, 194
AluI AGCT 4 cut(s) 135, 165, 170, 194
AlwI GGATC 1 cut(s) 357
AoxI GGCC 3 cut(s) 46, 80, 244
ApoI RAATTY 1 cut(s) 184
ArsI GACNNNNNNTTYG 2 cut(s) 318, 350
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 3 cut(s) 53, 81, 245
AvaII GGWCC 1 cut(s) 53
BalI TGGCCA 1 cut(s) 48
BccI CCATC 1 cut(s) 309
BciT130I CCWGG 3 cut(s) 79, 159, 413
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 269
BlpI GCTNAGC 1 cut(s) 166
Bme1390I CCNGG 3 cut(s) 79, 159, 413
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 3 cut(s) 53, 81, 245
BmrFI CCNGG 3 cut(s) 79, 159, 413
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 49, 77, 412
Bse1I ACTGG 2 cut(s) 96, 174
BseBI CCWGG 3 cut(s) 79, 159, 413
BseDI CCNNGG 3 cut(s) 49, 77, 412
BseGI GGATG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 96, 174
BshFI GGCC 3 cut(s) 48, 82, 246
BsnI GGCC 3 cut(s) 48, 82, 246
Bsp143I GATC 2 cut(s) 292, 349
Bsp1720I GCTNAGC 1 cut(s) 166
BspANI GGCC 3 cut(s) 48, 82, 246
BspCNI CTCAG 1 cut(s) 179
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 142
BsrI ACTGG 2 cut(s) 96, 174
BssECI CCNNGG 3 cut(s) 49, 77, 412
BssMI GATC 2 cut(s) 292, 349
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 3 cut(s) 79, 159, 413
Bst4CI ACNGT 1 cut(s) 231
Bst6I CTCTTC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 2 cut(s) 295, 352
BstMBI GATC 2 cut(s) 292, 349
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstNI CCWGG 3 cut(s) 79, 159, 413
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 3 cut(s) 77, 157, 411
BstXI CCANNNNNNTGG 1 cut(s) 464
BsuRI GGCC 3 cut(s) 48, 82, 246
BtsCI GGATG 1 cut(s) 320
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 181, 336
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 3 cut(s) 53, 81, 245
Csp6I GTAC 3 cut(s) 117, 357, 507
CviAII CATG 1 cut(s) 377
CviJI RGCY 8 cut(s) 48, 82, 135, 165, 170, 194, 220, 246
CviKI_1 RGCY 8 cut(s) 48, 82, 135, 165, 170, 194, 220, 246
CviQI GTAC 3 cut(s) 117, 357, 507
DdeI CTNAG 1 cut(s) 166
DpnI GATC 2 cut(s) 294, 351
DpnII GATC 2 cut(s) 292, 349
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 49
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 3 cut(s) 77, 157, 411
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 1 cut(s) 380
FaiI YATR 8 cut(s) 27, 39, 101, 144, 274, 276, 378, 465
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 1 cut(s) 376
FokI GGATG 1 cut(s) 327
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 3 cut(s) 48, 82, 246
HhaI GCGC 1 cut(s) 268
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 5 cut(s) 73, 86, 125, 154, 333
Hpy166II GTNNAC 2 cut(s) 305, 454
Hpy188I TCNGA 4 cut(s) 113, 153, 191, 256
Hpy8I GTNNAC 2 cut(s) 305, 454
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 1 cut(s) 231
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 1 cut(s) 166
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 2 cut(s) 292, 349
LguI GCTCTTC 1 cut(s) 142
LmnI GCTCC 2 cut(s) 175, 514
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 2 cut(s) 334, 407
MalI GATC 2 cut(s) 294, 351
MboI GATC 2 cut(s) 292, 349
MboII GAAGA 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 3 cut(s) 7, 184, 458
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 80, 342
MnlI CCTC 4 cut(s) 234, 256, 413, 469
Mox20I TGGCCA 1 cut(s) 48
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 48
MseI TTAA 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 48
MspR9I CCNGG 3 cut(s) 79, 159, 413
MvaI CCWGG 3 cut(s) 79, 159, 413
MwoI GCNNNNNNNGC 1 cut(s) 377
NdeII GATC 2 cut(s) 292, 349
NlaIII CATG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 334, 407
NspI RCATGY 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 73, 125, 154
PfoI TCCNGGA 1 cut(s) 157
PleI GAGTC 2 cut(s) 80, 341
PpsI GAGTC 2 cut(s) 80, 341
PpuMI RGGWCCY 1 cut(s) 53
PsiI TTATAA 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 53
Psp6I CCWGG 3 cut(s) 77, 157, 411
PspGI CCWGG 3 cut(s) 77, 157, 411
PspPI GGNCC 3 cut(s) 53, 81, 245
PspPPI RGGWCCY 1 cut(s) 53
RsaI GTAC 3 cut(s) 118, 358, 508
RsaNI GTAC 3 cut(s) 117, 357, 507
SapI GCTCTTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 363
Sau3AI GATC 2 cut(s) 292, 349
Sau96I GGNCC 3 cut(s) 53, 81, 245
SchI GAGTC 2 cut(s) 80, 342
ScrFI CCNGG 3 cut(s) 79, 159, 413
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 1 cut(s) 53
SpeI ACTAGT 1 cut(s) 427
Sse9I AATT 3 cut(s) 7, 184, 458
SspI AATATT 1 cut(s) 43
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 77, 157, 411
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 231
TaiI ACGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 399
TasI AATT 3 cut(s) 7, 184, 458
TfiI GAWTC 3 cut(s) 73, 125, 154
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 181, 343
TseFI GTSAC 2 cut(s) 334, 407
Tsp45I GTSAC 2 cut(s) 334, 407
TspDTI ATGAA 2 cut(s) 17, 26
TspRI CASTG 2 cut(s) 181, 343
VpaK11BI GGWCC 1 cut(s) 53
XapI RAATTY 1 cut(s) 184
XceI RCATGY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.