Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005261

Physical Location & Seq

Genomic Coordinates Reverse (-)

16228664 .. 16232575

Gene Structure 3912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1081400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGAAATTCGGGAAAGATTTCAAGAAACAAATGGTGCCTGAGTGGGCAGAAGCCTACATGGATTACAATGGTCTCAAGAGGATACTAAGGGAGCTCAGAGAATACAAGCGAAACAAGCACTCCGTAACACCCTCCAAAGACTTTGAGCGGAGACGTTCTCCCCCTGACAGAACCATCAGTGGGATTGAGCTACAATCTGGCAGTCTCCGGAGCAACGGAGATATCGAGGACCAAGTAATCGATGTCAACACATTGCACGGAGACGGTTGCAGAGAGGTTTACAAGACCAAGTTTCTGAGGCAATCCGAAGAAGGAGGAGATATTGAGGCGACATTTTTCCGAAAACTCGATCAAGAGCTCAACAAAGTCAATACCTTTTATAATGATAAAGTGGAGGAGGTGAAGAAAGAAGCAAATAATCTGGATAAGCAAATGGAGGCTTTGGTTGCACTGAGATTAAAAGTCAAGAAGCTTGATCAATACGGCTCTCGTTCAAAGAGACATAACAATTCGGCAGTACACCTGACAAGTAATAAGTCAAGTGCAGATGACAACTTAGGAAATGAGCCTACTGGTTTGACATCTGGATTGCAAGTAAATGAGGAGCATCGACGAGAACCAAGCACTGGTGGTTCAGAAGTCAATCCGAACCATGTCAAGAATTCTAGTAGCGATGGAAGAGAGTTATTGAAAACCTATACCCAAAAAGAAGATCCTATGGAGGTTCTTGAGCATGTGAGGATTACCAATACGCTTGAATCTCCAATATCCACCATAAAAGGTGTTTTCAAGGACTCTAGGGAAGAAGAGTTGAGCTTTGATAACGAGGAGCTGAAGAGAGTGGAAGAACGTTTAAGAGCTGCGTTCATTCAATTTTACCACCAGCTTCAACTGCTAAAGCACTACAGTTTTATGAACCTCTCAGCATTTTCGAAGATTATGAAAAAATATGAGAAGATTGCATCAAGGAGAGCGGCCAGATCATACATGCTAATAGTAGACAACTCTAATGTCGGCAGTTCTGATGAGGTCACTAACCTGTTGGAGAGAGTGGAGACTACCTTCATACAAAAATTTTCAAACTCAAATCGCAGGGAAGGTATGAAGTCACTGAGGCCAAAAGCTAAAAGAGAAGGGCAGTGTTTCTTTTCTGGCTGCTCAATTGCACTACTGGTAGCTATTGTTTTAAGCATTGAAGCTCGAAAACTACTGGATAAGGAGGAGGGTGCAGAGTACGTGGAAAACATTTTCCCACTTTACAGTTTATTTGGATACATCACTCTGCATATGCTCATGTATGCTGCAGATATATATTTCTGGAGGTGTTATCGGGTCAACTATGCCTTTATATTTGGTTTCAAGAAAGGAACGGAGCTGGGCTACCGTGAAGTTTTTCTCCTTGCCACTGGTCTTGCAGTACTTTCATTGGGTGGTTTCCTGGCAAACTTACACTTGGACTTGAGTGCTGAAAAATACAAGACAGTGACAGAATTGGTTCCCTTGGGCTTACTTGCTTTGGTCCTTGCCATCACCTTCTGCCCTTTCAAAATTATATACCATTCAAGTCGTTTCTTCTTCATTCGATCCTTATTCCACTGCACATGCGCCCCTCTTTACCCGGAGACATTTCCAGACTTTTTCTTGGCAGATCAGCTTACAAGCCAGGTGCAAGCCCTGAGGAGTTTTGTGTTATACGTTTGCTACTACGGTTTGGGGGAATCTTCAAGGAGGCAAAGCAAGTGCCGTAGTCATGGTCTCTACAATACGTTATATTTCATTATTGCTGTCGTACCATTTTGGATGCGCTTCCTACAGTGCATTCGTCGATTCTGTGATGAGAAGGATGTGAAGTACATTTTCAATGGTTTGAAGTACTTATCAACAATCGTTGCAGTTATTATACGAACTGTTTATGAAATGAAAAAGGGAAAGACATCCTGGATGGTGTTAGCTTTGATTAGCTCAGCTGTCGCAACGACAATGAATACGTATTGGGACATTGTAATGGACTGGGGCCTTCTGCGAAAGACATCAAAGAACAAGTATTTGAGAGATAGACTTGTAGTTCCACACAAGATCGTATACTTTGCAGCCATGGTCTTGAATGTAGTATTGAGACTAGCTTGGATGCAGTTGGTGTTGGAATTTAAATTGCGTACCGTTAATAAAGTGACAATAACAACTGTCATCGCCTTCCTAGAGGTCATTCGTCGTGGCATTTGGAGCTTCTTCAGGTTAGAGAACGAGCACTTGAACAATGTAGGCCAGTACCGCGCTTTCAAGTCAGTCCCTCTTCCTTTCAGTTACTACGATGAGGACGCTGACAAGGATGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0012505 GO:0016020 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

779

Amino Acids

90.17

Weight (kDa)

9.15

Isoelectric Point (pI)

44.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 330	2.3e-62	SPX domain
EXS	PF03124	416 - 751	2.1e-99	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69480 AT1G69480
fragaria_vesca	FvH4_4g30940 FvH4_4g30940 FvH4_4g30940
malus_domestica	MD00G1081200.v1.1 MD00G1081400.v1.1
prunus_persica	Prupe.1G278600_v2.0.a1 Prupe.1G278600_v2.0.a1 Prupe.1G278600_v2.0.a1 Prupe.1G278600_v2.0.a1 Prupe.1G278600_v2.0.a1
pyrus_communis	pycom13g06130
rosa_chinensis	RchiOBHm_Chr4g0439241 RchiOBHm_Chr4g0439281
rosa_laevigata	RLG00000006285
rosa_multiflora	Rmu_sc0006598.1_g000011 Rmu_ssc0000171.1_g000023 Rmu_ssc0000171.1_g000024
rosa_roxburghii	Rroxscaffold_5G00380240
rosa_rugosa	Rorug04G0317200
rosa_samantha	Rh4AG367800 Rh4AG367900 Rh4BG379700 Rh4CG394000 Rh4DG374200
rosa_wichuraiana	Rw0G005610 Rw0G011620 Rw5G028560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	381
AccB1I	GGYRCC	1 cut(s)	34
AccB7I	CCANNNNNTGG	1 cut(s)	626
AccBSI	CCGCTC	2 cut(s)	148, 974
AccI	GTMKAC	2 cut(s)	999, 2082
AccII	CGCG	1 cut(s)	2274
AccIII	TCCGGA	1 cut(s)	207
AciI	CCGC	3 cut(s)	148, 974, 2272
AclI	AACGTT	1 cut(s)	850
AclWI	GGATC	2 cut(s)	707, 1578
AcoI	YGGCCR	1 cut(s)	975
AcsI	RAATTY	4 cut(s)	5, 661, 1073, 2144
AcuI	CTGAAG	2 cut(s)	854, 2215
AfaI	GTAC	8 cut(s)	519, 1235, 1419, 1791, 1853, 1874, 2158, 2270
AfiI	CCNNNNNNNGG	2 cut(s)	180, 626
AjnI	CCWGG	3 cut(s)	1437, 1662, 1937
Alw21I	GWGCWC	3 cut(s)	96, 360, 2250
Alw26I	GTCTC	9 cut(s)	77, 145, 209, 255, 493, 1049, 1616, 1760, 2110
AlwI	GGATC	2 cut(s)	707, 1578
Aor13HI	TCCGGA	1 cut(s)	207
AoxI	GGCC	4 cut(s)	975, 1115, 2014, 2263
ApeKI	GCWGC	4 cut(s)	860, 1155, 1301, 2090
ApoI	RAATTY	4 cut(s)	5, 661, 1073, 2144
Asp700I	GAANNNNTTC	3 cut(s)	17, 1392, 1494
AspLEI	GCGC	3 cut(s)	1607, 1806, 2276
AspS9I	GGNCC	3 cut(s)	229, 1519, 2014
AsuC2I	CCSGG	1 cut(s)	1619
AsuHPI	GGTGA	2 cut(s)	412, 1522
AsuII	TTCGAA	1 cut(s)	932
AvaII	GGWCC	2 cut(s)	229, 1519
AxyI	CCTNAGG	1 cut(s)	1676
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	96, 360
Bbv12I	GWGCWC	3 cut(s)	96, 360, 2250
BbvI	GCAGC	4 cut(s)	847, 1142, 1288, 2102
BccI	CCATC	4 cut(s)	182, 668, 1535, 1936
BceAI	ACGGC	2 cut(s)	499, 1728
BciT130I	CCWGG	3 cut(s)	1439, 1664, 1939
BciVI	GTATCC	2 cut(s)	75, 1265
BclI	TGATCA	1 cut(s)	475
BcnI	CCSGG	1 cut(s)	1619
BcoDI	GTCTC	9 cut(s)	77, 145, 209, 255, 493, 1049, 1616, 1760, 2110
BfaI	CTAG	4 cut(s)	666, 798, 2120, 2198
BfmI	CTRYAG	3 cut(s)	904, 1302, 1811
BfuI	GTATCC	2 cut(s)	75, 1265
BisI	GCNGC	5 cut(s)	861, 975, 1156, 1302, 2091
BlpI	GCTNAGC	1 cut(s)	1963
BlsI	GCNGC	5 cut(s)	862, 976, 1157, 1303, 2092
BmcAI	AGTACT	2 cut(s)	1419, 1874
Bme1390I	CCNGG	4 cut(s)	1439, 1619, 1664, 1939
Bme18I	GGWCC	2 cut(s)	229, 1519
BmgT120I	GGNCC	3 cut(s)	229, 1519, 2014
BmiI	GGNNCC	3 cut(s)	36, 1497, 2015
BmrFI	CCNGG	4 cut(s)	1439, 1619, 1664, 1939
BmrI	ACTGGG	1 cut(s)	2020
BmsI	GCATC	4 cut(s)	616, 971, 1791, 2118
BmuI	ACTGGG	1 cut(s)	2020
BplI	GAGNNNNNCTC	2 cut(s)	142, 174
BpmI	CTGGAG	1 cut(s)	1339
Bpu1102I	GCTNAGC	1 cut(s)	1963
Bpu14I	TTCGAA	1 cut(s)	932
BpuEI	CTTGAG	3 cut(s)	59, 749, 1480
BpuMI	CCSGG	1 cut(s)	1619
Bsa29I	ATCGAT	1 cut(s)	240
BsaAI	YACGTR	2 cut(s)	1237, 1989
BsaI	GGTCTC	2 cut(s)	77, 1760
BsaJI	CCNNGG	2 cut(s)	1500, 2094
BsaWI	WCCGGW	1 cut(s)	207
BsaXI	ACNNNNNCTCC	2 cut(s)	104, 134
Bsc4I	CCNNNNNNNGG	2 cut(s)	180, 626
Bse1I	ACTGG	7 cut(s)	577, 631, 1176, 1215, 1411, 2015, 2266
Bse21I	CCTNAGG	1 cut(s)	1676
Bse3DI	GCAATG	1 cut(s)	251
BseAI	TCCGGA	1 cut(s)	207
BseBI	CCWGG	3 cut(s)	1439, 1664, 1939
BseCI	ATCGAT	1 cut(s)	240
BseDI	CCNNGG	2 cut(s)	1500, 2094
BseGI	GGATG	6 cut(s)	1806, 1849, 1934, 1947, 2133, 2335
BseLI	CCNNNNNNNGG	2 cut(s)	180, 626
BseMI	GCAATG	1 cut(s)	251
BseMII	CTCAG	8 cut(s)	30, 109, 287, 443, 936, 1103, 1667, 1977
BseNI	ACTGG	7 cut(s)	577, 631, 1176, 1215, 1411, 2015, 2266
BseRI	GAGGAG	6 cut(s)	330, 410, 617, 842, 1235, 1693
BseXI	GCAGC	4 cut(s)	847, 1142, 1288, 2102
BseYI	CCCAGC	1 cut(s)	1375
BsgI	GTGCAG	3 cut(s)	564, 1248, 1582
Bsh1236I	CGCG	1 cut(s)	2274
BshFI	GGCC	4 cut(s)	977, 1117, 2016, 2265
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	240
BsiHKAI	GWGCWC	3 cut(s)	96, 360, 2250
BsiSI	CCGG	2 cut(s)	208, 1619
BslFI	GGGAC	2 cut(s)	2009, 2273
BslI	CCNNNNNNNGG	2 cut(s)	180, 626
BsmAI	GTCTC	9 cut(s)	77, 145, 209, 255, 493, 1049, 1616, 1760, 2110
BsmBI	CGTCTC	2 cut(s)	145, 255
BsmFI	GGGAC	2 cut(s)	2009, 2273
BsmI	GAATGC	1 cut(s)	1818
BsnI	GGCC	4 cut(s)	977, 1117, 2016, 2265
Bso31I	GGTCTC	2 cut(s)	77, 1760
Bsp119I	TTCGAA	1 cut(s)	932
Bsp1286I	GDGCHC	3 cut(s)	96, 360, 2250
Bsp13I	TCCGGA	1 cut(s)	207
Bsp143I	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
Bsp1720I	GCTNAGC	1 cut(s)	1963
Bsp19I	CCATGG	1 cut(s)	2094
BspACI	CCGC	3 cut(s)	148, 974, 2272
BspANI	GGCC	4 cut(s)	977, 1117, 2016, 2265
BspCNI	CTCAG	8 cut(s)	31, 108, 288, 444, 935, 1104, 1668, 1976
BspDI	ATCGAT	1 cut(s)	240
BspEI	TCCGGA	1 cut(s)	207
BspFNI	CGCG	1 cut(s)	2274
BspLI	GGNNCC	3 cut(s)	36, 1497, 2015
BspMAI	CTGCAG	1 cut(s)	1306
BspPI	GGATC	2 cut(s)	707, 1578
BspT104I	TTCGAA	1 cut(s)	932
BspT107I	GGYRCC	1 cut(s)	34
BspTNI	GGTCTC	2 cut(s)	77, 1760
BsrBI	CCGCTC	2 cut(s)	148, 974
BsrDI	GCAATG	1 cut(s)	251
BsrI	ACTGG	7 cut(s)	577, 631, 1176, 1215, 1411, 2015, 2266
BssECI	CCNNGG	2 cut(s)	1500, 2094
BssMI	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
BssNAI	GTATAC	1 cut(s)	2083
BssT1I	CCWWGG	2 cut(s)	1500, 2094
Bst1107I	GTATAC	1 cut(s)	2083
Bst2UI	CCWGG	3 cut(s)	1439, 1664, 1939
Bst6I	CTCTTC	4 cut(s)	673, 801, 830, 2298
BstBAI	YACGTR	2 cut(s)	1237, 1989
BstBI	TTCGAA	1 cut(s)	932
BstC8I	GCNNGC	1 cut(s)	1671
BstDSI	CCRYGG	1 cut(s)	2094
BstF5I	GGATG	6 cut(s)	1806, 1849, 1934, 1947, 2133, 2335
BstFNI	CGCG	1 cut(s)	2274
BstHHI	GCGC	3 cut(s)	1607, 1806, 2276
BstKTI	GATC	7 cut(s)	352, 478, 715, 983, 1586, 1651, 2079
BstMAI	GTCTC	9 cut(s)	77, 145, 209, 255, 493, 1049, 1616, 1760, 2110
BstMBI	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
BstMWI	GCNNNNNNNGC	5 cut(s)	115, 446, 892, 2223, 2271
BstNI	CCWGG	3 cut(s)	1439, 1664, 1939
BstNSI	RCATGY	3 cut(s)	737, 991, 1605
BstSCI	CCNGG	4 cut(s)	1437, 1617, 1662, 1937
BstSFI	CTRYAG	3 cut(s)	904, 1302, 1811
BstSNI	TACGTA	1 cut(s)	1989
BstUI	CGCG	1 cut(s)	2274
BstV1I	GCAGC	4 cut(s)	847, 1142, 1288, 2102
BstX2I	RGATCY	1 cut(s)	712
BstYI	RGATCY	1 cut(s)	712
BstZ17I	GTATAC	1 cut(s)	2083
Bsu15I	ATCGAT	1 cut(s)	240
Bsu36I	CCTNAGG	1 cut(s)	1676
BsuI	GTATCC	2 cut(s)	75, 1265
BsuRI	GGCC	4 cut(s)	977, 1117, 2016, 2265
BsuTUI	ATCGAT	1 cut(s)	240
BtgI	CCRYGG	1 cut(s)	2094
BtgZI	GCGATG	2 cut(s)	687, 2173
BtsCI	GGATG	6 cut(s)	1806, 1849, 1934, 1947, 2133, 2335
BtsI	GCAGTG	2 cut(s)	1145, 1594
BtsIMutI	CAGTG	9 cut(s)	184, 449, 624, 1109, 1145, 1404, 1488, 1594, 1820
Cac8I	GCNNGC	1 cut(s)	1671
CfoI	GCGC	3 cut(s)	1607, 1806, 2276
Cfr13I	GGNCC	3 cut(s)	229, 1519, 2014
ClaI	ATCGAT	1 cut(s)	240
CseI	GACGC	1 cut(s)	2327
Csp6I	GTAC	8 cut(s)	518, 1234, 1418, 1790, 1852, 1873, 2157, 2269
CspCI	CAANNNNNGTGG	2 cut(s)	1393, 1428
CviAII	CATG	8 cut(s)	58, 653, 734, 988, 1294, 1602, 1751, 2095
CviQI	GTAC	8 cut(s)	518, 1234, 1418, 1790, 1852, 1873, 2157, 2269
DpnI	GATC	7 cut(s)	351, 477, 714, 982, 1585, 1650, 2078
DpnII	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
DraI	TTTAAA	1 cut(s)	2149
EaeI	YGGCCR	1 cut(s)	975
Eam1104I	CTCTTC	4 cut(s)	673, 801, 830, 2298
EarI	CTCTTC	4 cut(s)	673, 801, 830, 2298
Ecl136II	GAGCTC	2 cut(s)	94, 358
Eco105I	TACGTA	1 cut(s)	1989
Eco130I	CCWWGG	2 cut(s)	1500, 2094
Eco24I	GRGCYC	2 cut(s)	96, 360
Eco31I	GGTCTC	2 cut(s)	77, 1760
Eco32I	GATATC	1 cut(s)	223
Eco47I	GGWCC	2 cut(s)	229, 1519
Eco53kI	GAGCTC	2 cut(s)	94, 358
Eco57I	CTGAAG	2 cut(s)	854, 2215
Eco81I	CCTNAGG	1 cut(s)	1676
EcoICRI	GAGCTC	2 cut(s)	94, 358
EcoO109I	RGGNCCY	1 cut(s)	2014
EcoRI	GAATTC	1 cut(s)	661
EcoRII	CCWGG	3 cut(s)	1437, 1662, 1937
EcoRV	GATATC	1 cut(s)	223
EcoT14I	CCWWGG	2 cut(s)	1500, 2094
EcoT38I	GRGCYC	2 cut(s)	96, 360
ErhI	CCWWGG	2 cut(s)	1500, 2094
Esp3I	CGTCTC	2 cut(s)	145, 255
FaeI	CATG	8 cut(s)	61, 656, 737, 991, 1297, 1605, 1754, 2098
FaqI	GGGAC	2 cut(s)	2009, 2273
FatI	CATG	8 cut(s)	57, 652, 733, 987, 1293, 1601, 1750, 2094
FauNDI	CATATG	1 cut(s)	1287
FbaI	TGATCA	1 cut(s)	475
FblI	GTMKAC	2 cut(s)	999, 2082
Fnu4HI	GCNGC	5 cut(s)	861, 975, 1156, 1302, 2091
FokI	GGATG	5 cut(s)	1813, 1856, 1921, 1954, 2140
FriOI	GRGCYC	2 cut(s)	96, 360
Fsp4HI	GCNGC	5 cut(s)	861, 975, 1156, 1302, 2091
FspBI	CTAG	4 cut(s)	666, 798, 2120, 2198
GlaI	GCGC	3 cut(s)	1606, 1805, 2275
GluI	GCNGC	5 cut(s)	861, 975, 1156, 1302, 2091
GsaI	CCCAGC	1 cut(s)	1379
GsuI	CTGGAG	1 cut(s)	1339
HaeIII	GGCC	4 cut(s)	977, 1117, 2016, 2265
HapII	CCGG	2 cut(s)	208, 1619
HgaI	GACGC	1 cut(s)	2327
HhaI	GCGC	3 cut(s)	1607, 1806, 2276
Hin1II	CATG	8 cut(s)	61, 656, 737, 991, 1297, 1605, 1754, 2098
Hin6I	GCGC	3 cut(s)	1605, 1804, 2274
HinP1I	GCGC	3 cut(s)	1605, 1804, 2274
HincII	GTYRAC	2 cut(s)	247, 1336
HindII	GTYRAC	2 cut(s)	247, 1336
HindIII	AAGCTT	1 cut(s)	470
HinfI	GANTC	4 cut(s)	758, 794, 1718, 1827
HpaII	CCGG	2 cut(s)	208, 1619
HphI	GGTGA	2 cut(s)	412, 1522
Hpy166II	GTNNAC	6 cut(s)	247, 280, 520, 1000, 1336, 2083
Hpy188I	TCNGA	7 cut(s)	98, 297, 307, 341, 637, 648, 1024
Hpy8I	GTNNAC	6 cut(s)	247, 280, 520, 1000, 1336, 2083
Hpy99I	CGWCG	3 cut(s)	615, 1827, 2214
HpyAV	CCTTC	8 cut(s)	305, 1072, 1091, 1127, 1543, 1834, 2027, 2203
HpyCH4IV	ACGT	6 cut(s)	154, 850, 1236, 1695, 1766, 1988
HpyF10VI	GCNNNNNNNGC	5 cut(s)	115, 446, 892, 2223, 2271
HpySE526I	ACGT	6 cut(s)	154, 850, 1236, 1695, 1766, 1988
Hsp92II	CATG	8 cut(s)	61, 656, 737, 991, 1297, 1605, 1754, 2098
HspAI	GCGC	3 cut(s)	1605, 1804, 2274
Kpn2I	TCCGGA	1 cut(s)	207
Ksp22I	TGATCA	1 cut(s)	475
Kzo9I	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
LmnI	GCTCC	6 cut(s)	91, 210, 604, 829, 1372, 2223
Lsp1109I	GCAGC	4 cut(s)	847, 1142, 1288, 2102
LweI	GCATC	4 cut(s)	616, 971, 1791, 2118
MaeI	CTAG	4 cut(s)	666, 798, 2120, 2198
MaeII	ACGT	6 cut(s)	154, 850, 1236, 1695, 1766, 1988
MaeIII	GTNAC	6 cut(s)	124, 1030, 1107, 1483, 2170, 2303
MalI	GATC	7 cut(s)	351, 477, 714, 982, 1585, 1650, 2078
MbiI	CCGCTC	2 cut(s)	148, 974
MboI	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
MfeI	CAATTG	1 cut(s)	1161
MflI	RGATCY	1 cut(s)	712
MhlI	GDGCHC	3 cut(s)	96, 360, 2250
MlyI	GAGTC	1 cut(s)	788
MmeI	TCCRAC	2 cut(s)	1023, 2121
MroI	TCCGGA	1 cut(s)	207
MroXI	GAANNNNTTC	3 cut(s)	17, 1392, 1494
MseI	TTAA	5 cut(s)	458, 854, 1187, 2148, 2163
MslI	CAYNNNNRTG	1 cut(s)	2003
MspA1I	CMGCKG	1 cut(s)	1967
MspI	CCGG	2 cut(s)	208, 1619
MspR9I	CCNGG	4 cut(s)	1439, 1619, 1664, 1939
MunI	CAATTG	1 cut(s)	1161
Mva1269I	GAATGC	1 cut(s)	1818
MvaI	CCWGG	3 cut(s)	1439, 1664, 1939
MvnI	CGCG	1 cut(s)	2274
MwoI	GCNNNNNNNGC	5 cut(s)	115, 446, 892, 2223, 2271
NciI	CCSGG	1 cut(s)	1619
NcoI	CCATGG	1 cut(s)	2094
NdeI	CATATG	1 cut(s)	1287
NdeII	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
NlaIII	CATG	8 cut(s)	61, 656, 737, 991, 1297, 1605, 1754, 2098
NlaIV	GGNNCC	3 cut(s)	36, 1497, 2015
NmuCI	GTSAC	4 cut(s)	1030, 1107, 1483, 2170
NspI	RCATGY	3 cut(s)	737, 991, 1605
NspV	TTCGAA	1 cut(s)	932
PcsI	WCGNNNNNNNCGW	1 cut(s)	222
PctI	GAATGC	1 cut(s)	1818
PdmI	GAANNNNTTC	3 cut(s)	17, 1392, 1494
PfeI	GAWTC	3 cut(s)	758, 1718, 1827
PflMI	CCANNNNNTGG	1 cut(s)	626
PfoI	TCCNGGA	1 cut(s)	1937
PkrI	GCNGC	5 cut(s)	862, 976, 1157, 1303, 2092
PleI	GAGTC	1 cut(s)	788
PpsI	GAGTC	1 cut(s)	788
Ppu21I	YACGTR	2 cut(s)	1237, 1989
PsiI	TTATAA	1 cut(s)	381
Psp124BI	GAGCTC	2 cut(s)	96, 360
Psp1406I	AACGTT	1 cut(s)	850
Psp6I	CCWGG	3 cut(s)	1437, 1662, 1937
PspFI	CCCAGC	1 cut(s)	1375
PspGI	CCWGG	3 cut(s)	1437, 1662, 1937
PspN4I	GGNNCC	3 cut(s)	36, 1497, 2015
PspPI	GGNCC	3 cut(s)	229, 1519, 2014
PstI	CTGCAG	1 cut(s)	1306
PsuI	RGATCY	1 cut(s)	712
PvuII	CAGCTG	1 cut(s)	1967
RsaI	GTAC	8 cut(s)	519, 1235, 1419, 1791, 1853, 1874, 2158, 2270
RsaNI	GTAC	8 cut(s)	518, 1234, 1418, 1790, 1852, 1873, 2157, 2269
RseI	CAYNNNNRTG	1 cut(s)	2003
SacI	GAGCTC	2 cut(s)	96, 360
SaqAI	TTAA	5 cut(s)	458, 854, 1187, 2148, 2163
SatI	GCNGC	5 cut(s)	861, 975, 1156, 1302, 2091
Sau3AI	GATC	7 cut(s)	349, 475, 712, 980, 1583, 1648, 2076
Sau96I	GGNCC	3 cut(s)	229, 1519, 2014
ScaI	AGTACT	2 cut(s)	1419, 1874
SchI	GAGTC	1 cut(s)	788
ScrFI	CCNGG	4 cut(s)	1439, 1619, 1664, 1939
SduI	GDGCHC	3 cut(s)	96, 360, 2250
SfaNI	GCATC	4 cut(s)	616, 971, 1791, 2118
SfcI	CTRYAG	3 cut(s)	904, 1302, 1811
SfuI	TTCGAA	1 cut(s)	932
SinI	GGWCC	2 cut(s)	229, 1519
SmiI	ATTTAAAT	1 cut(s)	2149
SmiMI	CAYNNNNRTG	1 cut(s)	2003
SmlI	CTYRAG	3 cut(s)	74, 728, 1459
SmoI	CTYRAG	3 cut(s)	74, 728, 1459
SnaBI	TACGTA	1 cut(s)	1989
SsiI	CCGC	3 cut(s)	148, 974, 2272
SspMI	CTAG	4 cut(s)	666, 798, 2120, 2198
SstI	GAGCTC	2 cut(s)	96, 360
StyD4I	CCNGG	4 cut(s)	1437, 1617, 1662, 1937
StyI	CCWWGG	2 cut(s)	1500, 2094
SwaI	ATTTAAAT	1 cut(s)	2149
TaiI	ACGT	6 cut(s)	157, 853, 1239, 1698, 1769, 1991
TaqI	TCGA	8 cut(s)	225, 240, 348, 610, 932, 1201, 1582, 1825
TatI	WGTACW	4 cut(s)	517, 1417, 1851, 1872
TauI	GCSGC	1 cut(s)	977
TfiI	GAWTC	3 cut(s)	758, 1718, 1827
Tru1I	TTAA	5 cut(s)	458, 854, 1187, 2148, 2163
Tru9I	TTAA	5 cut(s)	458, 854, 1187, 2148, 2163
TscAI	CASTG	9 cut(s)	184, 456, 631, 1116, 1145, 1411, 1488, 1601, 1820
TseFI	GTSAC	4 cut(s)	1030, 1107, 1483, 2170
TseI	GCWGC	4 cut(s)	860, 1155, 1301, 2090
Tsp45I	GTSAC	4 cut(s)	1030, 1107, 1483, 2170
TspGWI	ACGGA	4 cut(s)	112, 231, 273, 1385
TspRI	CASTG	9 cut(s)	184, 456, 631, 1116, 1145, 1411, 1488, 1601, 1820
Van91I	CCANNNNNTGG	1 cut(s)	626
VpaK11BI	GGWCC	2 cut(s)	229, 1519
XapI	RAATTY	4 cut(s)	5, 661, 1073, 2144
XceI	RCATGY	3 cut(s)	737, 991, 1605
XmiI	GTMKAC	2 cut(s)	999, 2082
XmnI	GAANNNNTTC	3 cut(s)	17, 1392, 1494
XspI	CTAG	4 cut(s)	666, 798, 2120, 2198
ZrmI	AGTACT	2 cut(s)	1419, 1874

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.